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Oxidative Stress
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ALX-804-012 Revised 09-Jun-08
Monoclonal Antibody to PDI (RL90)
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SYNONYMS anti-Protein Disulfide-isomerase MAb (RL90)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Synthesis, Modification & Degradation Other Products
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ALX-804-012-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE: RL90
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Purified rat liver PDI (protein disulfide-isomerase).
SPECIFICITY: Recognizes human, mouse, rat, hamster and pig PDI. Detects a band of ~59kDa (rat liver extract) or ~61kDa (human liver extract) by Western blot.
APPLICATION: Flow Cytometry
Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections, frozen sections (1:100))
Immunoprecipitation
Western Blot (1:1’000)
Functional Application (see [4])
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
[1] Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies: C.S. Kaetzel, et al.; Biochem. J. 241, 39 (1987) Abstract
[2] Inhibition of a reductive function of the plasma membrane by bacitracin and antibodies against protein disulfide-isomerase: R. Mandel, et al.; PNAS 90, 4112 (1993) Abstract; Full Text
[3] Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interaction: H.J. Ryser, et al.; PNAS 91, 4559 (1994) Abstract; Full Text
[4] Sustained integrin ligation involves extracellular free sulfhydryls and enzymatically catalyzed disulfide exchange: J. Lahav, et al.; Blood 100, 2472 (2002) Abstract; Full Text
General Information

The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide-Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
BACKGROUND/TECHNICAL INFORMATION Immunohistochemical staining of PDI in rat intestine with Prod. No. ALX-804-012 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures, Prod. No. ALX-804-012 has been shown to inhibit the activity of PDI in vitro. Prod. No. ALX-804-012 has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.
Further Categories Containing This Product:
Monoclonal Antibodies • Endoplasmatic Reticulum Stress
 
 
ALX-804-579 Revised 18-Jun-08
Monoclonal Antibody to SEL1L (MSel1)
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SYNONYMS anti-Suppressor of lin-12-like Protein MAb (MSel1)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Endoplasmatic Reticulum Stress
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-579-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: MSel1
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In 150mM sodium chloride, pH 7.4, containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human SEL1L (suppressor of lin-12-like protein).
SPECIFICITY: Recognizes human and mouse SEL1L.
APPLICATION: Immunohistochemistry (frozen sections; paraffin sections)
Immunoprecipitation
Western Blot (10µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Do not freeze.
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Product Specific Literature References
Production of a monoclonal antibody directed against the recombinant SEL1L protein: R. Orlandi, et al.; J. Biol. Markers 17, 104 (2002) Abstract
SEL1L expression decreases breast tumor cell aggressiveness in vivo and in vitro: R. Orlandi, et al.; Cancer Res. 62, 567 (2002) Abstract; Full Text
General Information
SEL1L (suppressor of lin-12-like protein) is the human ortholog of the C. elegans sel-1 gene, a negative regulator of the lin-12/Notch family of proteins. Human SEL1L is a component of endoplasmic reticulum-associated degradation (ERAD), involved in ubiquitin-dependent degradation of misfolded ER proteins. In addition, SEL1L plays a role in cancer progression. Several studies provided significant evidence that increased SEL1L expression is linked to a decrease in tumor aggressiveness.
General Literature References
Complete cDNA sequence and genomic organization of a human pancreas-specific gene homologous to Caenorhabditis elegans sel-1: Y. Harada, et al.; J. Hum. Genet. 44, 330 (1999) Abstract
SEL1L a multifaceted protein playing a role in tumor progression: I. Biunno, et al.; J. Cell Physiol. 208, 23 (2006) Abstract
A different pathway in the endoplasmic reticulum stress-induced expression of human HRD1 and SEL1 genes: M. Kaneko, et al.; FEBS Lett. 581, 5355 (2007) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-248 Revised 01-Oct-07
Monoclonal Antibody to TRAF2 (human) (33A1293)
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SYNONYMS anti-Tumor Necrosis Factor (TNF) Receptor-associated Factor 2 (human) (33A1293)
PRODUCT LINE Inflammation
PRODUCT CATEGORY TRAFs/Related Products
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ALX-804-248-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 33A1293
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Recombinant human TRAF2 (tumor necrosis factor (TNF) receptor-associated factor 2) (aa 205-222).
SPECIFICITY: Recognizes human TRAF2. Detects a band of ~57kDa by Western blot.
APPLICATION: Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
Product Images
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Product Specific Literature References
Release of full-length 55-kDa TNF receptor 1 in exosome-like vesicles: a mechanism for generation of soluble cytokine receptors: F.I. Hawari, et al.; PNAS 101, 1297 (2004) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies • Endoplasmatic Reticulum Stress • Autophagy Other Products
 
 
ALX-385-016 Revised 02-Jun-08
Morin
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SYNONYMS 2’,3,4’,5,7-Pentahydroxyflavone
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Flavonoids/Related Products
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ALX-385-016-G001   1 g 16.00 USD Add To Cart
Product Specification
FORMULA: C15H10O7
MW: 302.2
CAS NUMBER: 480-16-0
MERCK INDEX: 14: 6269
RTECS: LK8749000
PURITY: ≥85%
APPEARANCE: White to brown powder.
SOLUBILITY: Soluble in methanol or 100% ethanol; slightly soluble in ether or acetic acid.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HAZARD: IRRITANT.

Product Description
Antioxidant flavonoid. Shows anti-proliferative and antitumor properties. Induces apoptosis. Induces cell cycle arrest at the G2/M phase. Anti-inflammatory compound. Suppresses NF-κB activation. Induces lipid peroxidation and DNA strand breaks.
Product Specific Literature References
Structure-antioxidant activity relationships of flavonoids and phenolic acids: C.A. Rice-Evans, et al.; Free Radical Biol. & Med. 20, 933 (1996), (Review) Abstract
Lipid peroxidation and DNA damage induced by morin and naringenin in isolated rat liver nuclei: S. C. Sahu & G. C. Gray; Food Chem. Toxicol. 35, 443 (1997) Abstract
Morin inhibits the growth of human leukemia HL-60 cells via cell cycle arrest and induction of apoptosis through mitochondria dependent pathway: H.M. Kuo, et al.; Anticancer Res. 27, 395 (2007) Abstract
Morin (3,5,7,2’,4’-Pentahydroxyflavone) abolishes nuclear factor-kappaB activation induced by various carcinogens and inflammatory stimuli, leading to suppression of nuclear factor-kappaB-regulated gene expression and up-regulation of apoptosi: S.K. Manna, et al.; Clin. Cancer Res. 13, 2290 (2007) Abstract
Further Categories Containing This Product:
Natural Products - NF-kB Pathway Inhibitors • NF-kB Pathway Inhibitors • Natural Products - Apoptosis Inducers & Inhibitors • Natural Products - Antitumor Reagents • Natural Products - Antioxidants • Natural Products - Anti-inflammatory Agents • Natural Products for Cell Cycle Research
 
 
ALX-430-134 Revised 16-Apr-08
MTSSL
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SYNONYMS (1-Oxyl-2,2,5,5-tetramethylpyrroline-3-methyl) methanethiosulfonate
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Spin Traps, Spin Probes & Spin Labels
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ALX-430-134-M010   10 mg 60.00 USD Add To Cart
ALX-430-134-M050   50 mg 240.00 USD Add To Cart
Product Specification
FORMULA: C10H18NO3S2
MW: 264.3
PURITY: ≥98% (HPLC)
APPEARANCE: Yellow crystalline solid.
SOLUBILITY: Soluble in water, methanol, 100% ethanol, DMSO, acetonitrile or acetone.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
Highly reactive thiol-specific spin label. Has been used to label cysteine residues in proteins (site-directed labelling, SDS-labelling). Allows protein structure and protein dynamics determination as well as the study of protein-protein and protein-oligonucleotide interactions.
Product Specific Literature References
A novel reversible thiol-specific spin label: papain active site labeling and inhibition: L.J. Berliner, et al.; Anal. Biochem. 119, 450 (1982) Abstract
Pressure-induced thermostabilization of glutamate dehydrogenase from the hyperthermophile Pyrococcus furiosus: M.M. Sun, et al.; Protein Sci. 8, 1056 (1999) Abstract
Protein structure determination using long-distance constraints from double-quantum coherence ESR: study of T4 lysozyme: P.P. Borbat, et al.; JACS 124, 5304 (2002) Abstract
Methods for study of protein dynamics and protein-protein interaction in protein-ubiquitination by electron paramagnetic resonance spectroscopy: H.J. Steinhoff; Front. Biosci. 7, c97 (2002) Abstract
Inter- and intra-molecular distances determined by EPR spectroscopy and site-directed spin labeling reveal protein-protein and protein-oligonucleotide interaction: H.J. Steinhoff; Biol. Chem. 385, 913 (2004) Abstract
Spontaneous refolding of the pore-forming Colicin A toxin upon membrane association as studied by X-band and W-band high-field electron paramagnetic resonance spectroscopy: A. Savitski, et al.; J. Phys. Chem. B 108, 9541 (2004)
Calcium structural transition of human cardiac troponin C in reconstituted muscle fibres as studied by site-directed spin labelling: M. Nakamura, et al.; J. Mol. Biol. 348, 127 (2005) Abstract
 
 
ALX-200-600 Revised 12-Nov-07
Myeloperoxidase (human leukocytes)
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents/Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-200-600-U010   10 U 90.00 USD Add To Cart
ALX-200-600-U025   25 U 165.00 USD Add To Cart
ALX-200-600-5010   5x10 U 350.00 USD Add To Cart
ALX-200-600-5025   5x25 U 650.00 USD Add To Cart
Product Specification
MW: ~118kDa.
CAS NUMBER: 9003-99-0
EC: 1.11.1.7
SOURCE/HOST: Isolated from human leukocytes. Negative for HBsAG-, HCV- and HIV-antibodies.
PURITY: ≥95% (SDS-PAGE). 
FORMULATION: Lyophilized from 0.02M sodium acetate buffer, pH 6.0.
RECONSTITUTION: Reconstitute with 1ml distilled water.
SPECIFIC ACTIVITY: ~250-300U/mg protein. One unit is defined as the amount of enzyme that produces an increase in absorbance at 470nm of 1.0 per min. at 25°C, pH 7.0 (calculated from the initial rate of reaction using 1% guaiacol as substrate; initial H2O2 concentration 6mmol/l).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Specific Literature References
Guinea pig heterophil and eosinophil peroxidase: R.K. Desser, et al.; Arch. Biochem. Biophys. 148, 452 (1972) Abstract
Oxidation of amino acids and peptides in reaction with myeloperoxidase, chloride and hydrogen peroxide: R. Drozdz, et al.; Biochim. Biophys. Acta 957, 47 (1988) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P05164: Myeloperoxidase (human) (precursor)
Further Categories Containing This Product:
Natural Proteins • Enzymes
 
 
ALX-385-012 Revised 19-Nov-07
Myricetin
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SYNONYMS 3,3',4',5,5',7-Hexahydroxyflavone
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Flavonoids/Related Products
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ALX-385-012-M010   10 mg 35.00 USD Add To Cart
ALX-385-012-M050   50 mg 140.00 USD Add To Cart
Product Specification
FORMULA: C15H10O8
MW: 318.2
CAS NUMBER: 529-44-2
MERCK INDEX: 14: 6332
RTECS: LK8646000
PURITY: ≥97%
APPEARANCE: Light yellow-green crystals.
SOLUBILITY: Soluble in alcohol; slightly soluble in water (warm); insoluble in acetic acid.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HANDLING: Protect from light.

Product Description
Antioxidant flavonoid. Has antitumor and chemopreventive properties. Anti-inflammatory. Inhibits NF-κB activation. 
Product Specific Literature References
Structure-antioxidant activity relationships of flavonoids and phenolic acids: C.A. Rice-Evans, et al.; Free Radical Biol. & Med. 20, 933 (1996), (Review) Abstract
Biological effects of myricetin: K. C. Ong & H. E. Khoo; Gen. Pharmacol. 29, 121 (1997), (Review) Abstract
In vitro cytotoxicity of glyco-S-nitrosothiols. a novel class of nitric oxide donors: H. Babich & H.L. Zuckerbraun; Toxicol. In Vitro 15, 181 (2001) Abstract
Suppression of TNFalpha-mediated NFkappaB activity by myricetin and other flavonoids through downregulating the activity of IKK in ECV304 cells: S. H. Tsai, et al.; J. Cell Biochem. 74, 606 (1999) Abstract
Myricetin inhibits matrix metalloproteinase 2 protein expression and enzyme activity in colorectal carcinoma cells: C.H. Ko, et al.; Mol. Cancer Ther. 4, 281 (2005) Abstract
Mitochondrial-dependent, reactive oxygen species-independent apoptosis by myricetin: roles of protein kinase C, cytochrome c, and caspase cascade: C.H. Ko, et al.; Biochem. Pharmacol. 69, 913 (2005) Abstract
Inhibition of Mammalian thioredoxin reductase by some flavonoids: implications for myricetin and quercetin anticancer activity: J. Lu, et al.; Cancer Res. 66, 4410 (2006) Abstract
Myricetin is a novel natural inhibitor of neoplastic cell transformation and MEK1: K.W. Lee, et al.; Carcinogenesis 28, 1918 (2007) Abstract
Further Categories Containing This Product:
Natural Products - NF-kB Pathway Inhibitors • NF-kB Pathway Inhibitors • Natural Products - Antioxidants • Natural Products - Antitumor Reagents • Natural Products - Anti-inflammatory Agents • Active Substances from Fruit and Vegetables
 
 
ALX-201-235 Revised 17-Aug-06
NAD Kinase (full length) (active) (human) (recombinant) (His)
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SYNONYMS NADK (full length) (active) (human) (recombinant) (His)
Poly(P)/ATP NAD Kinase (full length) (active) (human) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism/Related Products
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ALX-201-235-C050   50 µg 320.00 USD Add To Cart
Product Specification
EC: 2.7.1.23
SOURCE/HOST: Produced in E. coli. Human NADK is fused at the N-terminus to a His-tag.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Free of any nucleotide converting side-activities.
FORMULATION: Ammonium sulphate suspension.
RECONSTITUTION: Prepare working aliquots by centrifugation and dissolve the pellet in 50mM TRIS/HCl pH 7.5, 150mM NaCl, 1mM DTT.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 weeks when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Structural and functional characterization of human NAD kinase: F. Lerner, et al.; BBRC 288, 69 (2001) Abstract
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins • Enzymes • Kinases Other Products
 
 
ALX-201-236 Revised 17-Aug-06
NAD Kinase (NT truncated) (active) (human) (recombinant) (His)
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SYNONYMS NADK (NT truncated) (active) (human) (recombinant) (His)
Poly(P)/ATP NAD Kinase (NT truncated) (active) (human) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism/Related Products
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ALX-201-236-C050   50 µg 260.00 USD Add To Cart
Product Specification
EC: 2.7.1.23
SOURCE/HOST: Produced in E. coli. Human NADK (aa 64-446) is fused at the N-terminus to a His-tag.
QUANTITY: Sufficient for the preparation of >10µmol of NADP from NAD and ATP in 1ml of a 10mM solution.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Free of any nucleotide converting side-activities.
FORMULATION: Liquid. Ammonium sulphate suspension.
RECONSTITUTION: Prepare working aliquots by centrifugation and dissolve the pellet in 50mM TRIS/HCl pH 7.5, 150mM NaCl, 1mM DTT.
SPECIFIC ACTIVITY: ≥3U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol of NADP per min.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 3 months when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Structural and functional characterization of human NAD kinase: F. Lerner, et al.; BBRC 288, 69 (2001) Abstract
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins • Enzymes • Kinases Other Products
 
 
ALX-480-003 Revised 10-Dec-04