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Protein Synthesis, Modification & Degradation
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ALX-804-215 Revised 24-Feb-06
Monoclonal Antibody to NF-ATc2 (25A10.D6.D2)
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SYNONYMS anti-Nuclear Factor of Activated T Cells, Cytoplasmic 2 MAb (25A10.D6.D2)
anti-NFAT1 MAb (25A10.D6.D2)
anti-NFATP MAb (25A10.D6.D2)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Nuclear Factor of Activated T Cells [NF-AT]
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-215-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 25A10.D6.D2
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Purified antibody in PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 51-69 (A51ISSPSGLAYPDD
VLDYGL69) of mouse nuclear factor of activated T cells, cytoplasmic 2 (NF-ATc2) conserved in all three isoforms A, B and C.
SPECIFICITY: Recognizes human, mouse and rat NF-ATc2. Detects bands of ~140kDa (phosphorylated NF-ATc2 in resting immune cells) and ~120kDa (fully-dephosphorylated NF-ATc2 in stimulated cells) by Western blot. Does not cross-react with NF-ATc1.
APPLICATION: Gel Supershift Assays
Immunocytochemistry (1µg/ml)
Immunoprecipitation
Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-163-009.
Product Specific Literature References
The calcineurin-NFAT pathway and muscle fiber-type gene expression: S.J. Swoap, et al.; Am. J. Physiol. Cell Physiol. 279, C915 (2000) Abstract; Full Text
Identification and characterization of a novel nuclear factor of activated T-cells-1 isoform expressed in mouse brain: S. Plyte, et al.; J. Biol. Chem. 276, 14350 (2001) Abstract; Full Text
The basis for TCR-mediated regulation of the IL-2 receptor alpha chain gene: role of widely separated regulatory elements: H.P. Kim & W. Leonard; EMBO J. 21, 3051 (2002) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Note: Confusion may arise regarding NFAT protein versus gene names.
According to Swiss-Prot, the nomenclature is as follows:
Nuclear Factor of Activated T Cells, Cytoplasmic 1 (NF-ATc1)
Gene Names: NFATC1 or NFATC or NFAT2
Nuclear Factor of Activated T Cells, Cytoplasmic 2 (NF-ATc2)
Gene Names: NFATC2 or NFATP or NFAT1
Nuclear Factor of Activated T Cells, Cytoplasmic 3 (NF-ATc3)
Gene Names: NFATC3 or NFATX or NFAT4
Nuclear Factor of Activated T Cells, Cytoplasmic 4 (NF-ATc4)
Gene Names: NFATC4 or NFAT3
Nuclear Factor of Activated T Cells (NF-AT5)
Gene Names: NFAT5
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-043 Revised 04-Dec-04
Monoclonal Antibody to NF-κB (human) (NF3A11)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-043-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: NF3A11
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: 100µg of protein G purified antibody in 100µl Tris/Glycine buffer containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human NF-κB (p50 subunit).
SPECIFICITY: Recognizes p50 subunit of human NF-κB (epitope in the region aa 191-197).
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-044 Revised 04-Dec-04
Monoclonal Antibody to NF-κB (human) (NF5E6)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-044-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: NF5E6
ISOTYPE: Rat IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Protein G purified antibody in Tris/Glycine buffer containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human NF-κB (p50 subunit).
SPECIFICITY: Recognizes p50 subunit of human NF-κB (epitope in the region aa 1-15).
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-361 Revised 09-May-07
Monoclonal Antibody to NF-κB (p65) (112A1021)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
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ALX-804-361-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 112A1021
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 526-539 of human NF-κB (p65 subunit) .
SPECIFICITY: Recognizes human and mouse NF-κB (p65 subunit ). Detects a band of ~65kDa by Western blot.
APPLICATION: Flow Cyctometry (2.5µg/ml) (see [1])
Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
Product Specific Literature References
[1] Human keratinocytes respond to interleukin-18: implication for the course of chronic inflammatory skin diseases: M. Wittmann, et al.; J. Invest. Dermatol. 124, 1225 (2005) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-852 Revised 28-Apr-08
Monoclonal Antibody to Omentin 1 (Saly-2)
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SYNONYMS anti-Intelectin-1 MAb (Saly-2)
anti-Intestinal Lactoferrin Receptor MAb (Saly-2)
anti-Galactofuranose-binding Lectin MAb (Saly-2)
anti-Endothelial Lectin HL-1 MAb (Saly-2)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Omentin / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-852-C100   100 µg 360.00 USD Add To Cart
ALX-804-852B-C100 Biotin 100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
CLONE: Saly-2
ISOTYPE: Mouse IgG2a
SOURCE/HOST: Purified from concentrated hybridoma tissue cell culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human omentin 1 (intelectin-1) (aa 17-231).
SPECIFICITY: Recognizes human omentin 1 and rat omentin. Cross-reacts with human omentin 2.
APPLICATION: ELISA (detection)
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
General Information
Omentin 1 (also named intelectin-1, endothelial lectin HL-1 and intestinal lactoferrin receptor) has been recently identified as a novel visceral fat (or omental fat) depot-specific adipokine. Omentin 1 is a secreted protein of ~38 kDa containing a fibrinogen-related domain. Omentin 1 is highly expressed in the omental fat, and much less in intestine, lung and heart. Addition of recombinant omentin in vitro did not affect basal, but enhanced insulin-stimulated glucose uptake in subcutaneous as well as in omental human adipocytes. Omentin 1 triggers AKT signalling in the absence of insulin.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action: R.Z. Yang, et al.; Am. J. Physiol. Endocrinol. Metab. 290, E1253 (2006) Abstract
Related Products
Further Categories Containing This Product:
PI(3)K-Akt-mTOR Pathway Other Products • Monoclonal Antibodies
 
 
ALX-804-850 Revised 16-Aug-07
Monoclonal Antibody to Omentin 1 (human) (Saly-1)
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SYNONYMS anti-Intelectin-1 (human) MAb (Saly-1)
anti-Intestinal Lactoferrin Receptor (human) MAb (Saly-1)
anti-Galactofuranose-binding Lectin (human) MAb (Saly-1)
anti-Endothelial Lectin HL-1 (human) MAb (Saly-1)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Omentin / Related Products
Ordering Information
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ALX-804-850-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Saly-1
ISOTYPE: Mouse IgG2b
SOURCE/HOST: Purified from concentrated hybridoma tissue cell culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human omentin 1 (intelectin-1) (aa 17-231).
SPECIFICITY: Recognizes human omentin 1.
APPLICATION: ELISA (capture)
Immunohistochemistry (paraffin sections)
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
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General Information
Omentin 1 (also named intelectin-1, endothelial lectin HL-1 and intestinal lactoferrin receptor) has been recently identified as a novel visceral fat (or omental fat) depot-specific adipokine. Omentin 1 is a secreted protein of ~38 kDa containing a fibrinogen-related domain. Omentin 1 is highly expressed in the omental fat, and much less in intestine, lung and heart. Addition of recombinant omentin in vitro did not affect basal, but enhanced insulin-stimulated glucose uptake in subcutaneous as well as in omental human adipocytes. Omentin 1 triggers AKT signalling in the absence of insulin.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action: R.Z. Yang, et al.; Am. J. Physiol. Endocrinol. Metab. 290, E1253 (2006) Abstract
Related Products
Further Categories Containing This Product:
PI(3)K-Akt-mTOR Pathway Other Products • Monoclonal Antibodies
 
 
ALX-804-186 Revised 04-Sep-07
Monoclonal Antibody to p53 (phosphorylated) (pSer392) (9F4)
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Antibodies
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-186-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 9F4
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1 ml distilled water (15 min, RT).
IMMUNOGEN: Synthetic peptide corresponding to a portion of p53 phosphorylated at Ser392.
SPECIFICITY: Recognizes activated human p53 phosphorylated at Ser392. Does not cross-react with the non-phosphorylated form of p53 or with unrelated serine-phosphorylated proteins.
APPLICATION: Western Blot: 1µg/ml for AP/BCIP/NBT (MTT) detection; 0.1µg/ml for HRPO/ECL detection. Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies • Tumor Markers
 
 
ALX-801-060 Revised 20-May-08
Monoclonal Antibody to p53 (human) (BP53-12)
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SYNONYMS anti-p53 (human) MAb (BP53-12)
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Antibodies
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ALX-801-060-C100   100 µg 210.00 USD Add To Cart
ALX-801-060F-C100 FITC 100 µg 315.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Monkey
CLONE: BP53-12
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Recombinant human wild-type p53 protein.
SPECIFICITY: Antibody BP53-12 detects a denaturation resistant epitope (aa 16-25) in the human p53 protein.
APPLICATION: BP53-12:
ELISA
Immunocytochemistry
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot (1-2µg/ml)
BP53-12-FITC:
Flow Cytometry
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
Product Specific Literature References
Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies: J. Bartek, et al.; J. Pathol. 169, 27 (1993) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-013 Revised 29-Sep-05
Monoclonal Antibody to PDI (RL77)
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SYNONYMS anti-Protein Disulfide-isomerase MAb (RL77)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Synthesis, Modification & Degradation Other Products
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ALX-804-013-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE: RL77
ISOTYPE: Mouse IgG2b
FORMULATION: Liquid. Ascites fluid containing 0.05% sodium azide.
IMMUNOGEN: Purified rat liver PDI (protein disulphide-isomerase).
SPECIFICITY: Recognizes human, mouse, rat and hamster and Xenopus laevis PDI. Detects a band of ~59kDa (rat liver PDI) or ~61KDa (human liver PDI).
APPLICATION: Immunohistochemistry (paraffin sections (1:100))
Immunoprecipitation (inhibits activity of PDI in vitro)
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies: C.S. Kaetzel, et al.; Biochem. J. 241, 39 (1987) Abstract
General Information
The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide-Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
Further Categories Containing This Product:
Endoplasmatic Reticulum Stress • Monoclonal Antibodies
 
 
ALX-804-012 Revised 09-Jun-08
Monoclonal Antibody to PDI (RL90)
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SYNONYMS anti-Protein Disulfide-isomerase MAb (RL90)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Synthesis, Modification & Degradation Other Products
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ALX-804-012-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE: RL90
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.