ALEXIS Biochemicals: Monoclonal Antibody to α-Gal Epitope (Galα1-3Galβ1-4GlcNAc-R) (M86)

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ALX-801-090

Revised 28-Oct-08

Monoclonal Antibody to α-Gal Epitope (Galα1-3Galβ1-4GlcNAc-R) (M86)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Xenotransplantation

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-801-090-L002		3 ml	320.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse Rat Pig Others
CLONE:	M86
ISOTYPE:	Mouse IgM
FORMULATION:	Liquid. Supernatant antibody.
IMMUNOGEN:	Rabbit red blood cell membrane.
SPECIFICITY:	Recognizes synthetic and naturally produced mouse, rat and pig Galα1-3Gal epitopes on glycoproteins and glycolipids. Does not cross-react with β-Gal glycoproteins or BSA.
APPLICATION:	ELISA Flow Cytometry Immunohistochemistry (paraffin sections) Western Blot Functional Application (can be used for cytotoxicity assays for α-Gal specific pathways) Note: Do not use non-fat milk for blocking! Many blocking agents from non-human mammal sources will cross-react with the antibody. An antibody dilution of 1:5 is recommended. Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 3 years when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Description

Widely described antibody against α-galactose residues with broad cross-reactivity among different species. Useful for measuring the α-Gal epitope expression on cells, glycolipids and glycoproteins, characterization of hyperacute rejection (HAR) in organ and tissue transplantations and monitoring xenotransplantation experiments. The IgM isotype mimicks in vivo reactions and can be used for cytotoxicity assays for α-Gal specific pathways (addition or presence of complement necessary). Ideal as a high-throughput screening tool for inhibitors of antibody induced cytotoxicity.
Organ transplantation from pig to human result in HAR. Humans naturally produce large quantities of anti-α-Gal antibodies, which represent 1-3% of all circulating immunoglobulins and are produced by about 1% of all B cells. When pig organs or tissues are transplanted into the human body, the IgM isotype of anti-Gal binds to α-Gal epitopes, which causes activation of the complement cascade, resulting in cell lysis. This rapid activation of complement by anti-Gal IgM is an immunological barrier that poses the greatest risk of initiating HAR. The monoclonal antibody M86 to Galα1-3Gal epitopes developed by U. Galili, et al. is an essential tool in the study of human xenotransplantation and related research.

Product Specific Literature References

A sensitive assay for measuring alpha-Gal epitope expression on cells by a monoclonal anti-Gal antibody: U. Galili, et al.; Transplantation 65, 1129 (1998) Abstract
Regulation of natural killer cell-mediated swine endothelial cell lysis through genetic remodeling of a glycoantigen: S. Miyagawa, et al.; J. Biochem. 126, 1067 (1999) Abstract
alpha-Gal Oligosaccharides: Chemistry and Potential Biomedical Application: A. Janczuk, et al.; Current Med. Chem. 6, 155 (1999) Abstract
Mechanism of delayed rejection in transgenic pig-to-primate cardiac xenotransplantation: R.H. Chen, et al.; J. Surg. Res. 90, 119 (2000) Abstract
Xenotransplantation: in vitro analysis of synthetic alpha-galactosyl inhibitors of human anti-Galalpha1-->3Gal IgM and IgG antibodies: R. Rieben, et al.; Glycobiology 10, 141 (2000) Abstract
Differential immune responses to alpha-gal epitopes on xenografts and allografts: implications for accommodation in xenotransplantation: M. Tanemura, et al.; J. Clin. Invest. 105, 301 (2000) Abstract
Differential expression of alpha-Gal epitopes on pig and mouse organs: M. Tanemura & U. Galili; Transplant. Proc. 32, 843 (2000) Abstract
The alpha-gal epitope (Gal alpha 1-3Gal beta 1-4GlcNAc-R) in xenotransplantation: U. Galili; Biochemie 83, 557 (2001) Abstract
Remodeling of the Major Pig Xenoantigen by N-Actetylglucosaminyltransferase III in Transgenic Pig: S. Miyagawa, et al.; J. Biol. Chem. 276, 39310 (2001) Abstract; Full Text
Synthesis of α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R) on human tumor cells by recombinant α1,3galactosyltransferase produced in Pichia pastoris: Z.C. Chen, et al.; Glycobiology 11, 577 (2001) Abstract; Full Text
Differential expression of Galalpha1,3Gal epitopes on fetal and adult porcine hematopoietic cells: S. Gojo, et al.; Xenotransplantation 9, 297 (2002) Abstract; Full Text
Expression of alpha-gal epitopes on HeLa cells transduced wtih adenovirus containing alpha-1,3galactosyltransferase cDNA: L. Deriy, et al.; Glycobiology 12, 135 (2002) Abstract
Lack of Galactose-alpha-1,3-Galactose Expression on Porcine Endothelial Cells Prevents Complement-Induced Lysis but Not Direct Xenogeneic NK Cytotoxicity: B.C. Baumann, et al.; J. Immunol. 172, 6460 (2004) Abstract

Further Categories Containing This Product:

CD40 & CD40L [CD154] / Related Products • Monoclonal Antibodies

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