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Peroxynitrite / Scavengers / Detection
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Items 12 of 12
ALX-270-097 Revised 07-Sep-06
Ebselen
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SYNONYMS 2-Phenyl-1,2-benzisoselenazol-3-(2H)-one
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Peroxynitrite / Scavengers / Detection
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ALX-270-097-M001   1 mg 12.00 USD Add To Cart
ALX-270-097-M005   5 mg 20.00 USD Add To Cart
ALX-270-097-M025   25 mg 60.00 USD Add To Cart
Product Specification
FORMULA: C13H9NOSe
MW: 274.2
CAS NUMBER: 60940-34-3
PURITY: ≥98% (NMR)
APPEARANCE: White to off-white crystalline solid.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
Selenium-based inhibitor of protein kinase C, NADPH, 5-lipoxygenase, cyclooxygenase (COX) and NADPH oxidase. Anti-inflammatory antioxidant. Mimics glutathione peroxidase. Inhibits oxidative modifications of low density lipoproteins (LDL).
Product Specific Literature References
A novel biologically active seleno-organic compound--III. Effects of PZ 51 (Ebselen) on glutathione peroxidase and secretory activities of mouse macrophages: M.J. Parnham & S. Kindt; Biochem. Pharmacol. 33, 3247 (1984) Abstract
Seleno-organic compounds and the therapy of hydroperoxide-linked pathological conditions: M.J. Parnham & E. Graf; Biochem. Pharmacol. 36, 3095 (1987) Abstract
Kinetic mechanism and substrate specificity of glutathione peroxidase activity of ebselen (PZ51): M. Maiorino, et al.; Biochem. Pharmacol. 37, 2267 (1988) Abstract
Studies on the anti-inflammatory activity of ebselen. Ebselen interferes with granulocyte oxidative burst by dual inhibition of NADPH oxidase and protein kinase C?: I.A. Cotgreave, et al.; Biochem. Pharmacol. 38, 649 (1989) Abstract
T. Nikawa, et al.; Biochem. Pharmacol. 47, 1007 (1994) Abstract
Effects of ebselen and probucol on oxidative modifications of lipid and protein of low density lipoprotein induced by free radicals: N. Noguchi, et al.; Biochim. Biophys. Acta 1213, 176 (1994) Abstract
Strong inhibition of mammalian lipoxygenases by the antiinflammatory seleno-organic compound ebselen in the absence of glutathione: C. Schewe, et al.; Biochem. Pharmacol. 48, 65 (1994) Abstract
Ebselen: a glutathione peroxidase mimic: H. Sies; Meth. Enzymol. 234, 476 (1994) Abstract
Molecular actions of ebselen-an antiinflammatory antioxidant: T. Schewe; Gen. Pharmacol. 26, 1153 (1995) Abstract
Ebselen: H. Sies; Meth. Enzymol. 252, 341 (1995) Abstract
Ebselen as a glutathione peroxidase mimic and as a scavenger of peroxynitrite: H. Sies & H. Masumoto; Adv. Pharmacol. 38, 229 (1997) Abstract
Free radical generation by selenium compounds and their prooxidant toxicity: J.E. Spallholz; Biomed. Environ. Sci. 10, 260 (1997) Abstract
Protection against peroxynitrite by selenoproteins: H. Sies, et al.; Z. Naturforsch. [C] 53, 228 (1998) Abstract
Ebselen protects mice against T cell-dependent, TNF-mediated apoptotic liver injury: G. Tiegs, et al.; J. Pharmacol. Exp. Ther. 287, 1098 (1998) Abstract; Full Text
Further Categories Containing This Product:
Antioxidants, Flavonoids & Free Radical Scavengers Other Products • PKC Inhibitors • Malaria / Related Products • Lipoxygenases / Related Products • Anti-inflammatory Agents Other Products • COX Inhibitors
 
 
ALX-270-252 Revised 03-Mar-05
MEG . sodium succinate
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SYNONYMS Mercaptoethylguanidine . sodium succinate
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY NOS Inhibitors (NOS Induction & Enzyme Activity)
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ALX-270-252-M010   10 mg 95.00 USD Add To Cart
Product Specification
FORMULA: C3H10N3S . C4H4O4Na
MW: 120.2 . 139.1
PURITY: ≥90%
APPEARANCE: White solid.
SOLUBILITY: Soluble in water.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Keep under inert gas. Keep cool and dry. Protect from moisture.

Product Description
Inhibitor of inducible nitric oxide synthase (iNOS; NOS II). Peroxynitrite scavenger.
Product Specific Literature References
Spontaneous rearrangement of aminoalkylisothioureas into mercaptoalkylguanidines, a novel class of nitric oxide synthase inhibitors with selectivity towards the inducible isoform: G.J. Southan, et al.; Br. J. Pharmacol. 117, 619 (1996) Abstract
The inhibitory effects of mercaptoalkylguanidines on cyclo-oxygenase activity: B. Zingarelli, et al.; Br. J. Pharmacol. 120, 357 (1997) Abstract
Mercaptoethylguanidine and guanidine inhibitors of nitric-oxide synthase react with peroxynitrite and protect against peroxynitrite-induced oxidative damage: C. Szabó, et al.; J. Biol. Chem. 272, 9030 (1997) Abstract; Full Text
Antiinflammatory effects of mercaptoethylguanidine, a combined inhibitor of nitric oxide synthase and peroxynitrite scavenger, in carrageenan-induced models of inflammation: S. Cuzzocrea, et al.; Free Radic. Biol. Med. 24, 450 (1998) Abstract
Protective effects of MEG, a selective inhibitor of inducible nitric oxide synthase, in ligature-induced periodontitis in the rat [published erratum appears in Br. J. Pharmacol. 123, 741 (1998)]: Z. Lohinai, et al.; Br. J. Pharmacol. 123, 353 (1998) Abstract
Mercaptoethylguanidine, a combined inhibitor of nitric oxide synthase and peroxynitrite scavenger, reduces trinitrobenzene sulfonic acid-induced colonic damage in rats: B. Zingarelli, et al.; J. Pharmacol. Exp. Ther. 287, 1048 (1998) Abstract
Modulation of peroxynitrite- and hypochlorous acid-induced inactivation of alpha1-antiproteinase by mercaptoethylguanidine: M. Whiteman, et al.; Br. J. Pharmacol. 126, 1646 (1999) Abstract
Further Categories Containing This Product:
Peroxynitrite / Scavengers / Detection
 
 
ALX-400-036 Revised 31-Oct-06
Peroxynitrite . tetramethylammonium
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SYNONYMS ONOO- . C4H12N
Oxoperoxonitrate(1-) . C4H12N
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Peroxynitrite / Scavengers / Detection
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ALX-400-036-L001   1 ml 152.00 USD Add To Cart
ALX-400-036-5001   5x1 ml 475.00 USD Add To Cart
Product Specification
FORMULA: NO3 . C4H12N
MW: 62.0 . 74.1
CAS NUMBER: 14042-01-4
CONCENTRATION: ~10mM
FORMULATION: Liquid. In 0.01M KOH.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 1 month after receipt when stored at -80°C.
HANDLING: Protect from light.
HAZARD: OXIDIZING. CORROSIVE.

Product Description
Produced from the reaction of nitrogen monoxide with tetramethylammonium superoxide according to the method of D.S. Bohle, et al. described in Meth. Enzymol. 269, 302 (1996), and dissolved in 0.01M potassium hydroxide (KOH). This formulation of peroxynitrite has a low nitrite content (~1%), no hydrogen peroxide.
Product Specific Literature References
DNA damage induced by peroxynitrite: subsequent biological effects: C. Szabo & H. Ohshima; Nitric Oxide 1, 373 (1997), (Review) Abstract
Peroxynitrite: a biologically significant oxidant: M.P. Murphy, et al.; Gen. Pharmacol. 31, 179 (1998), (Review) Abstract
Peroxynitrite: an endogenous oxidizing and nitrating agent: C. Ducrocq, et al.; Cell. Mol. Life Sci. 55, 1068 (1999), (Review) Abstract
The chemistry of DNA damage from nitric oxide and peroxynitrite: S. Burney, et al.; Mutat. Res. 424, 37 (1999), (Review) Abstract
Reactions of *NO, *NO2 and peroxynitrite in membranes: physiological implications: S.P. Goss, et al.; Free Radic. Res. 31, 597 (1999), (Review) Abstract
Peroxynitrite: reactive, invasive and enigmatic: J.T. Groves; Curr. Opin. Chem. Biol. 3, 226 (1999), (Review) Abstract
Nitric oxide and peroxynitrite. The ugly, the uglier and the not so good: a personal view of recent controversies: B. Halliwell, et al.; Free Radic. Res. 31, 651 (1999), (Review) Abstract
Assessment of peroxynitrite scavengers in vitro: B. Halliwell, et al.; Meth. Enzymol. 301, 333 (1999), (Review) Abstract
Chemistry of peroxynitrite and its relevance to biological systems: W.H. Koppenol; Met. Ions Biol. Syst. 36, 597 (1999), (Review) Abstract
Conformation of peroxynitrite: determination by crystallographic analysis: M. Worle, et al.; Chem. Res. Toxicol 12, 305 (1999) Abstract
Peroxynitrite scavenging by metalloporphyrins and thiolates: J.P. Crow; Free Radic. Biol. Med. 28, 1487 (2000), (Review) Abstract
Interaction of peroxynitrite with selenoproteins and glutathione peroxidase mimics: H. Sies & G.E. Arteel; Free Radic. Biol. Med. 28, 1451 (2000), (Review) Abstract
Reactions of Peroxynitrite with Phenolic and Carbonyl Compounds: Flavonoids are not Scavengers of Peroxynitrite: S. Tibi & W.H. Koppenol; Helv. Chim. Acta 83, 2412 (2000)
BCL-2 protects peroxynitrite-treated thymocytes from poly(ADP-ribose) synthase (PARS)-independent apoptotic but not from PARS-mediated necrotic cell death: L. Virag & C. Szabo; Free Radic. Biol. Med. 29, 704 (2000) Abstract
Peroxynitrite promotes mitochondrial permeability transition-dependent rapid u937 cell necrosis: survivors proliferate with kinetics superimposable on those of untreated cells: P. Sestili, et al.; Free Radic Res. 34, 513 (2001) Abstract
Peroxynitrite-induced cytotoxicity: mechanism and opportunities for intervention: L. Virag, et al.; Toxicol. Lett. 140-141, 113 (2003), (Review) Abstract
Preventing nitrite contamination in tetramethylammonium peroxynitrite solutions: P. Latal, et al.; Inorg. Chem. 43, 6519 (2004) Abstract
Further Categories Containing This Product:
PARP Other Products • DNA Repair Other Products
 
 
ALX-430-092 Revised 28-Jan-05
FeTMPyP . pentachloride
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SYNONYMS Iron (III) tetrakis(N-methyl-4'-pyridyl)porphyrin . 5Cl
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Porphyrins
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ALX-430-092-M010   10 mg 43.00 USD Add To Cart
Product Specification
FORMULA: C44H36FeN8 . 5Cl
MW: 732.7 . 177.3
CAS NUMBER: 133314-07-5
PURITY: ≥98%
APPEARANCE: Black solid.
SOLUBILITY: Soluble in aqueous buffers or isotonic saline.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 2 years after receipt when stored at –20°C.
HANDLING: Protect from light and moisture.

Product Description
Synthetic porphyrin complexed with iron which acts as a peroxynitrite decomposition complex. Catalyzes the isomerization of peroxynitrite to nitrate under physiological conditions. Effectively prevents the oxidation of lipid components by peroxynitrite. Also catalytically decomposes superoxide radicals. Irridation of the aqueous solution at 335nm releases hydroxyl radicals. It can also serve as a potential drug for reactive oxygen species (ROS) detoxification.
Product Specific Literature References
Peroxynitrite decomposition catalysts: M.K. Stern, et al.; JACS 118, 8735 (1996)
Amphiphilic peroxynitrite decomposition catalysts in liposomal assemblies: J.A. Hunt, et al.; Chem. Biol. 4, 845 (1997) Abstract
Peroxynitrite rapidly permeates phospholipid membranes: S.S. Marla, et al.; PNAS 94, 14243 (1997) Abstract
Characterization of the cytoprotective action of peroxynitrite decomposition catalysts: T.P. Misko, et al.; J. Biol. Chem. 273, 15646 (1998) Abstract; Full Text
Protective effects of a superoxide dismutase mimetic and peroxynitrite decomposition catalysts in endotoxin-induced intestinal damage: D. Salvemini, et al.; Br. J. Pharmacol. 127, 685 (1999) Abstract
Methamphetamine generates peroxynitrite and produces dopaminergic neurotoxicity in mice: protective effects of peroxynitrite decomposition catalyst: S.Z. Imam, et al.; Brain Res. 837, 15 (1999) Abstract
Further Categories Containing This Product:
Peroxynitrite / Scavengers / Detection
 
 
ALX-610-021 Revised 04-Nov-05
Dihydrorhodamine 123
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SYNONYMS DHR
PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-610-021-M005   5 mg 153.00 USD Add To Cart
ALX-610-021-M010   10 mg 272.00 USD Add To Cart
Product Specification
FORMULA: C21H18N2O3
MW: 346.4
CAS NUMBER: 109244-58-8
PURITY: ≥98%
APPEARANCE: Pink crystalline solid.
SOLUBILITY: Soluble in organic solvents such as ethanol, DMSO or dimethyl formamide.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Stock solutions are stable for at least 6 months when stored at -20°C.

Product Description
Sensitive indicator of peroxynitrite formation. DHR is oxidized by peroxynitrite to the highly fluorescent product rhodamine. Formation of rhodamine can be monitored by fluorescence spectroscopy (Ex(max): 500nm, Em(max): 536nm), or by absorbance spectroscopy at 500nm (ε=78'800M-1cm-1). Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR.
Product Specific Literature References
Peroxynitrite-mediated oxidation of dihydrorhodamine 123: N.W. Kooy, et al.; Free Radic. Biol. Med. 16, 149 (1994) Abstract
Attenuation of oxidation and nitration reactions of peroxynitrite by selenomethionine, selenocystine and ebselen: K. Briviba, et al.; Biochem. J. 319, 13 (1996) Abstract
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species: J.P. Crow; Nitric Oxide 1, 145 (1997) Abstract
Glutathione peroxidase protects against peroxynitrite-mediated oxidations. A new function for selenoproteins as peroxynitrite reductase: H. Sies, et al.; J. Biol. Chem. 272, 27812 (1997) Abstract; Full Text
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate: S.L. Hempel, et al.; Free Radic. Biol. Med. 27, 146 (1999) Abstract
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123: H. Ischiropoulos, et al.; Meth. Enzymol. 301, 367 (1999) Abstract
Further Categories Containing This Product:
Respiratory Chain Other Products • Peroxynitrite / Scavengers / Detection
 
 
ALX-610-022 Revised 25-Apr-08
2',7'-Dichlorodihydrofluorescein diacetate
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SYNONYMS DCDHF diacetate
2',7'-Dichlorofluorescein diacetate
PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-610-022-M050   50 mg 43.00 USD Add To Cart
Product Specification
FORMULA: C24H16O7Cl2
MW: 487.3
CAS NUMBER: 4091-99-0
PURITY: ≥95%
APPEARANCE: White to off-white solid.
SOLUBILITY: 25mg/ml soluble in DMSO, 100% ethanol or dimethyl formamide; sparingly soluble in aqueous buffers.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stock solution in organic solvents is stable for at least 6 months if stored at -20°C. For maximum solubility in aqueous buffers, dissolve directly in 0.1M disodium carbonate (7.7mg/ml) and then immediately diluted with PBS, pH 7.2, to the desired concentration or pH. Upon neutralization of the solution the concentration may not be greater than 1mg/ml. We do not recommend storing the aqueous solution for more than one day.

Product Description
Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalyzed cleavage of the diacetate groups, DCDHF is oxidized by peroxynitrite to the highly fluorescent product dichlorofluorescein (DCF). Formation of DCF can be monitored by fluorescence spectroscopy (Ex(max): 502nm, Em(max): 523nm), or by absorbance spectroscopy at 500nm (ε=79,500M-1cm-1). Neither nitric oxide, superoxide nor hydrogen peroxide alone appear to oxidize DCDHF.
Product Specific Literature References
Peroxynitrite-mediated oxidation of dihydrorhodamine 123: N.W. Kooy, et al.; Free Radic. Biol. Med. 16, 149 (1994) Abstract
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species: J.P. Crow; Nitric Oxide 1, 145 (1997) Abstract
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate: S.L. Hempel, et al.; Free Radic. Biol. Med. 27, 146 (1999) Abstract
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123: H. Ischiropoulos, et al.; Meth. Enzymol. 301, 367 (1999) Abstract
Further Categories Containing This Product:
Peroxynitrite / Scavengers / Detection
 
 
ALX-620-062 Revised 01-Jun-06
2-(4-Dehydroxy)coelenterazine
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SYNONYMS Coelenterazine h
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Peroxynitrite / Scavengers / Detection
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ALX-620-062-C050   50 µg 110.00 USD Add To Cart
Product Specification
FORMULA: C26H21N3O2
MW: 407.5
PURITY: ≥95%
APPEARANCE: Yellow to orange solid.
SOLUBILITY: Soluble in 100% ethanol or methanol; slightly soluble in water. Avoid DMSO as product is unstable in this solvent.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light. Keep under inert gas. Keep calcium free when stored in solution.
HAZARD: IRRITANT.

Product Description
Derivative of coelenterazine (Prod. No. ALX-620-063) with ~20 times higher luminescence intensity.
Product Specific Literature References
Semi-synthetic aequorins with improved sensitivity to Ca2+ ions: O. Shimomura, et al.; Biochem. J. 261, 913 (1989) Abstract
Preparation and handling of aequorin solutions for the measurement of cellular Ca2+: O. Shimomura; Cell Calcium 12, 635 (1991) Abstract
Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins: M.R. Knight, et al.; J. Cell. Biol. 121, 83 (1993) Abstract
Intracellular Ca2+ signals in Dictyostelium chemotaxis are mediated exclusively by Ca2+ influx: T. Nebl & P.R. Fisher; J. Cell Sci. 110, 2845 (1997) Abstract
Further Categories Containing This Product:
Calcium Indicators / Probes • Antioxidants, Flavonoids & Free Radical Scavengers Other Products • Dyes / Stains / Fluorescent Probes / Fluorescent Labels • Oxidative Stress Markers & Reagents / Related Products
 
 
ALX-620-063 Revised 03-Feb-05
Coelenterazine
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SYNONYMS 2-[(4-Hydroxyphenyl)methyl]-6-(4-hydroxyphenyl)-8-(phenylmethyl)-imidazo [1,2-a] pyrazin-3-(7H)-one
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Peroxynitrite / Scavengers / Detection
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ALX-620-063-C050   50 µg 110.00 USD Add To Cart
Product Specification
FORMULA: C26H21N3O3
MW: 423.5
CAS NUMBER: 55779-48-1
PURITY: ≥95%
APPEARANCE: Brown solid.
SOLUBILITY: Slightly soluble in methanol or ethanol. Avoid DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light. Keep under inert gas. Keep calcium free when stored in solution.

Product Description
Cell permeable, very sensitive and specific chemiluminescence probe of the superoxide anion and peroxynitrite. When oxidized by oxygen it emits blue light at 446nm when Ca2+ binds to the complex. Powerful antioxidant. See also 2-(4-Dehydroxy)coelenterazine (Prod. No. ALX-620-062).
Product Specific Literature References
Use of calcium-regulated photoproteins as intracellular Ca2+ indicators: J.R. Blinks; Meth. Enzymol. 172, 164 (1989) Abstract
Slow calcium waves accompany cytokinesis in medaka fish eggs: R.A. Fluck, et al.; J. Cell Biol. 115, 1259 (1991) Abstract
Coelenterazine is a superoxide anion-sensitive chemiluminescent probe: its usefulness in the assay of respiratory burst in neutrophils: M. Lucas & F. Solano; Anal. Biochem. 206, 273 (1992) Abstract
Aequorin-expressing mammalian cell lines used to report Ca2+ mobilization: D. Button & M. Brownstein; Cell Calcium 14, 663 (1993) Abstract
Imaging [Ca2+]i with aequorin using a photon imaging detector: A.L. Miller, et al.; Meth. Cell Biol. 40, 305 (1994) Abstract
Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA: S. Saran, et al.; FEBS Lett. 337, 43 (1994) Abstract
The origins of marine bioluminescence: turning oxygen defence mechanisms into deep-sea communication tools: J.F. Rees, et al.; J. Exp. Biol. 201, 1211 (1998) Abstract; Full Text
Chemiluminescent detection of oxidants in vascular tissue. Lucigenin but not coelenterazine enhances superoxide formation: M.M. Tarpey, et al.; Circ. Res. 84, 1203 (1999) Abstract
Further Categories Containing This Product:
Calcium Indicators / Probes • Antioxidants, Flavonoids & Free Radical Scavengers Other Products • Luciferin & Luciferase / Related Products • Dyes / Stains / Fluorescent Probes / Fluorescent Labels • Oxidative Stress Markers & Reagents / Related Products
 
 
ALX-620-072 Revised 03-Sep-08
ZnAF-2 . tetrahydrochloride
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SYNONYMS 6-[N-[N',N'-bis(2-Pyridinylmethyl)-2-aminoethyl]amino-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one . 4 HCl
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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ALX-620-072-M001   1 mg 380.00 USD Add To Cart
Product Specification
FORMULA: C34H28N4O5 . 4HCl
MW: 572.6 .145.8
CONCENTRATION: ~5mM
PURITY: ≥98% (HPLC)
FORM