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Oxidative Stress
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ALX-210-524 Revised 29-Sep-05
Polyclonal Antibody to Peroxiredoxin I
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SYNONYMS anti-PrxI PAb
anti-Thioredoxin Peroxidase II PAb
anti-PAG PAb
anti-NEKF-A PAb
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Peroxiredoxins / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-524-R100   100 µl 355.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
PURITY DETAIL: Crude antiserum.
FORMULATION: Liquid. Contains 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 104-115 (L104VSDPKRTIAQD115) of human PrxI (peroxiredoxin I).
SPECIFICITY: Recognizes human, mouse and rat PrxI. Detects a band of ~26kDa by Western blot.   Does not cross-react with PrxII, PrxIII or PrxIV.
APPLICATION: Immunocytochemistry (1:500)
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Images
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General Literature References
Nonredundant antioxidant defense by multiple two-cysteine peroxiredoxins in human prostate cancer cells: C. Shen and C. Nathan; Mol. Med. 8, 95 (2002) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-525 Revised 23-Feb-05
Polyclonal Antibody to Peroxiredoxin II (human)
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SYNONYMS anti-PrxII (human) PAb
anti-Thioredoxin Peroxidase I (human) PAb
anti-PRP (human) PAb
anti-TSA (human) PAb
anti-NEKF-B (human) PAb
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Peroxiredoxins / Related Products
Ordering Information
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ALX-210-525-R100   100 µl 355.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Crude antiserum. Contains no preservatives.
IMMUNOGEN: Synthetic peptide corresponding to aa 103-114 (L103LADVTRRLSED114) of human PrxII (peroxiredoxin II).
SPECIFICITY: Recognizes human PrxII. Does not cross-react with PrxI, PrxIII or PrxIV.
APPLICATION: Western Blot (10'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
General Literature References
Nonredundant antioxidant defense by multiple two-cysteine peroxiredoxins in human prostate cancer cells: C. Shen and C. Nathan; Mol. Med. 8, 95 (2002) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-526 Revised 23-Feb-05
Polyclonal Antibody to Peroxiredoxin III (human)
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SYNONYMS anti-PrxIII (human) PAb
anti-NEKF-C (human) PAb
anti-Thioredoxin Peroxidase III (human) PAb
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Peroxiredoxins / Related Products
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ALX-210-526-R100   100 µl 355.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Crude antiserum. Contains no preservatives.
IMMUNOGEN: Recombinant human PrxIII (peroxiredoxin III).
SPECIFICITY: Recognizes human PrxIII. Does not cross-react with PrxI, PrxII or PrxIV.
APPLICATION: Western Blot (1:10'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
General Literature References
Nonredundant antioxidant defense by multiple two-cysteine peroxiredoxins in human prostate cancer cells: C. Shen and C. Nathan; Mol. Med. 8, 95 (2002) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-527 Revised 23-Feb-05
Polyclonal Antibody to Peroxiredoxin IV (human)
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SYNONYMS anti-PrxIV (human) PAb
anti-NEKF-D (human) PAb
anti-Thioredoxin Peroxidase IV (human) PAb
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Peroxiredoxins / Related Products
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ALX-210-527-R100   100 µl 355.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Crude antiserum. Contains no preservatives.
IMMUNOGEN: Recombinant human PrxIV (peroxiredoxin IV).
SPECIFICITY: Recognizes human PrxIV. Does not cross-react with PrxI, PrxII or PrxIII.
APPLICATION: Western Blot (1:10'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
General Literature References
Nonredundant antioxidant defense by multiple two-cysteine peroxiredoxins in human prostate cancer cells: C. Shen and C. Nathan; Mol. Med. 8, 95 (2002) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-530 Revised 13-Oct-08
Polyclonal Antibody to DMPO
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Spin Traps, Spin Probes & Spin Labels
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ALX-210-530-R100   100 µl 290.00 USD Add To Cart
Product Specification
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Contains no stabilizers or preservatives.
IMMUNOGEN: DMPO-octanoic acid conjugated to ovalbumin.
SPECIFICITY: Recognizes DMPO (Prod. No. ALX-430-090), DMPO-octanoic acid and DMPO-protein adducts. Does not cross react with non-adducted proteins.
APPLICATION: ELISA: 1:5’000. Reactive against DMPO-protein adducts in dilutions of 1:100-10’000 followed by reaction with alkaline phosphatase conjugated, affinity purified anti-rabbit IgG (goat).
Immunocytochemistry
Western Blot:
 1:5’000.
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. Dilute only prior to immediate use.
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Product Specific Literature References
Immunological identification of the heart myoglobin radical formed by hydrogen peroxide: C.D. Detweiler, et al.; Free Radic. Biol. Med. 33, 364 (2002) Abstract
Immunochemical detection of hemoglobin-derived radicals formed by reaction with hydrogen peroxide: involvement of a protein-tyrosyl radical: D.C. Ramirez, et al.; Free Radic. Biol. Med. 34, 830 (2003) Abstract
UVA-ketoprofen-induced hemoglobin radicals detected by immuno-spin trapping: Y.Y. He, et al.; Photochem. Photobiol. 77, 585 (2003) Abstract
Reaction of human hemoglobin with peroxynitrite. Isomerization to nitrate and secondary formation of protein radicals: N. Romero, et al.; J. Biol. Chem. 278, 44049 (2003) Abstract
Identification of free radicals on hemoglobin from its self-peroxidation using mass spectrometry and immuno-spin trapping: observation of a histidinyl radical: L. Deterding; J. Biol. Chem. 279, 11600 (2004) Abstract
Using anti-5,5-dimethyl-1-pyrroline N-oxide (anti-DMPO) to detect protein radicals in time and space with immuno-spin trapping: R.P. Mason; Free Radic. Biol. Med. 36, 1214 (2004) Abstract
Protein radical formation during lactoperoxidase-mediated oxidation of the suicide substrate glutathione: immunochemical detection of a lactoperoxidase radical-derived 5,5-dimethyl-1-pyrroline N-oxide nitrone adduct: Q. Guo, et al.; J. Biol. Chem. 279, 13272 (2004) Abstract
Involvement of protein radical, protein aggregation, and effects on NO metabolism in the hypochlorite-mediated oxidation of mitochondrial cytochrome c: Y.R. Chen, et al.; Free Radic. Biol. Med. 37, 1591 (2004) Abstract
Novel identification of a sulfur-centered, radical-derived 5,5-dimethyl-1-pyrroline N-oxide nitrone adduct formed from the oxidation of DTT by LC/ELISA, LC/electrospray ionization-MS, and LC/tandem MS: Q. Guo, et al.; Chem. Res. Toxicol. 17, 1481 (2004) Abstract
Mechanism of hydrogen peroxide-induced Cu,Zn-superoxide dismutase-centered radical formation as explored by immuno-spin trapping: the role of copper- and carbonate radical anion-mediated oxidations: D.C. Ramirez, et al.; Free Radic. Biol. Med. 38, 201 (2005) Abstract
Copper-catalyzed protein oxidation and its modulation by carbon dioxide: enhancement of protein radicals in cells: D.C. Ramirez, et al.; J. Biol. Chem. 280, 27402 (2005) Abstract
Identification of the myoglobin tyrosyl radical by immuno-spin trapping and its dimerization: C.D. Detweiler, et al.; Free Radic. Biol. Med. 38, 969 (2005) Abstract
Immuno-spin trapping: detection of protein-centered radicals: D.C. Ramirez, et al.; Curr. Prot. in Toxicology 2, 17.7.1 (2005)
Cytochrome c acts as a cardiolipin oxygenase required for release of proapoptotic factors: V.E. Kagan, et al.; Nat. Chem. Biol. 1, 223 (2005) Abstract
Superoxide generation from mitochondrial NADH dehydrogenase induces self-inactivation with specific protein radical formation: Y.R. Chen, et al.; J. Biol. Chem. 280, 37339 (2005) Abstract; Full Text
Immunochemical detection of nitric oxide and nitrogen dioxide trapping of the tyrosyl radical and the resulting nitrotyrosine in sperm whale myoglobin: K. Nakai and R.P. Mason; Free Radic. Biol. Med. 39, 1050 (2005) Abstract
Immuno-spin trapping of DNA radicals: D.C. Ramirez, et al.; Nat. Methods 3, 123 (2006) Abstract
Immuno-spin trapping of hemoglobin and myoglobin radicals derived from nitrite-mediated oxidation: A. Keszler, et al.; Free Radic. Biol. Med. 40, 507 (2006) Abstract
Immuno-spin trapping: A breakthrough for sensitive detection of protein-derived radicals: R. Radi, et al.; Free Radic. Biol. Med. 41, 416 (2006)
Protein radical formation on thyroid peroxidase during turnover: M. Ehrenshaft, et al.; Free Radic. Biol. Med. 41, 422 (2006)
Immunolocalization of hypochlorite-induced, catalase-bound free radical formation in mouse hepatocytes: M.G. Bonini et al.; Free Rad. Biol. Med. 42, 530 (2007)
Mitochondrial Complex II in the Post-ischemic Heart: Oxidative injury and the role or protein S-Glutathionylation: Y.R. Chen, et al.; J. Biol. Chem. 282, 32640 (2007) Abstract; Full Text
Further Categories Containing This Product:
DNA Damage & Repair Other ProductsPolyclonal Antibodies
 
 
ALX-210-205 Revised 15-May-07
Polyclonal Antibody to Peroxisomal Membrane Protein 70 (mouse)
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SYNONYMS anti-PMP70 (mouse) PAb
anti-ABCD3 (mouse) PAb
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Cell Stress, Oxidative Stress & Redox Signalling Other Products
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ALX-210-205-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 644-659 (CN644YEFKKITED TVEFGS659) of C-terminal rat PMP70 (peroxisomal membrane protein 70). This sequence is completely conserved in mouse PMP70 and differs from human PMP70 by a single aa substitution.
SPECIFICITY: Recognizes mouse PMP70. Detects a band of ~70kDa by Western blot.
APPLICATION: Flow Cytometry
Western Blot (1:500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-165-036.
General Information
Peroxisomes are single membrane organelles which are involved in many important biochemical pathways and are found in almost all eukaryotic cells. Various toxins including phenols, alcohols, and aldehydes are targeted for modification by peroxisomes. Long chain fatty acid metabolism via β-oxidation is also mediated by this organelle. Loss of peroxisomal functions may result in diseases such as Zellweger syndrome, hyperpipecolic acidemia, neonatal adrenoleukodystrophy, and infantile Refsum’s disease. Peroxisomal membrane protein 70 (PMP70) is an abundant, integral membrane protein of the peroxisome. This protein is induced by treatment with hypolipidemic agents in parallel with peroxisome proliferation and stimulation of the peroxisomal β-oxidation enzymes.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-872 Revised 11-Nov-05
Polyclonal Antibody to Bradykinin B2 Receptor
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SYNONYMS anti-B2R PAb
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Bradykinin Receptor / Related Products
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ALX-210-872-R200   200 µl 195.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Undiluted serum containing 0.01% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 356-391 of human B2R (bradykinin B2 receptor).
SPECIFICITY: Recognizes human, mouse and rat B2R.
APPLICATION: Immunoprecipitation (use 2-5µl of undiluted antiserum per precipitate)
Western Blot (1:100-1:500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Ligand-induced phosphorylation/dephosphorylation of the endogenous bradykinin B2 receptor from human fibroblasts: A. Blaukat, et al.; J. Biol. Chem. 271, 32366 (1996) Abstract; Full Text
Overexpression and functional characterization of kinin receptors reveal subtype-specific phosphorylation: A. Blaukat, et al.; Biochemistry 38, 1300 (1999) Abstract
Bradykinin-induced internalization of the human B2 receptor requires phosphorylation of three serine and two threonine residues at its carboxyl tail: A. Pizard, et al.; J. Biol. Chem. 274, 13738 (1999) Abstract; Full Text
Determination of bradykinin B2 receptor in vivo phosphorylation sites and their role in receptor function: A. Blaukat, et al.; J. Biol. Chem. 276, 40431 (2001) Abstract; Full Text
Functional specialization of calreticulin domains: K. Nakamura, et al.; J. Cell Biol. 154, 961 (2001) Abstract
 
 
ALX-210-947 Revised 13-Oct-08
Polyclonal Antibody to TIGAR (AT122)
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SYNONYMS anti-Tp53-induced Glycolysis and Apoptosis Regulator PAb (AT122)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Glycobiology Other Products
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ALX-210-947-C100   100 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human TIGAR (aa 1-270) fused at the N-terminus to a tag.
SPECIFICITY: Recognizes human and mouse TIGAR.
APPLICATION: Western Blot (1:1’000)
Note: Does not work for immunoprecipitation.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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General Information

The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. TIGAR (TP53-induced glycolysis and apoptosis regulator) has been identified as a novel p53-inducible gene. TIGAR expression decreased fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis. In addition, TIGAR expression lowered intracellular reactive oxygen species (ROS) levels and protected cells from p53-induced apoptosis. Bensaad et al., proposed that TIGAR modulates the apoptotic response to p53, thereby allowing cells to survive mild or transient stress.

MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
TIGAR, a p53-inducible regulator of glycolysis and apoptosis: K. Bensaad, et al.; Cell 126, 107 (2006) Abstract; Full Text
 
 
ALX-210-425 Revised 23-Oct-08
Polyclonal Antibody to Nampt/Visfatin (human)
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SYNONYMS anti-Nicotinamide Phosphoribosyltransferase (human) PAb
anti-PBEF1 (human) PAb
anti-Pre-B-Cell Colony Enhancing Factor 1 (human) PAb
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin / Nampt / PBEF / Related Products
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ALX-210-425-C100   100 µg 310.00 USD Add To Cart
ALX-210-425B-C050 Biotin 50 µg 310.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Purified IgG.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human Nampt/visfatin.
SPECIFICITY: Recognizes human Nampt/visfatin. Weakly cross-reacts with mouse Nampt/visfatin.
APPLICATION: ELISA (direct and indirect: 1:2'000-1:5'000).
Western Blot (1:2'000-1:5'000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1: S.K. Bae, et al.; FEBS Lett. 580, 4105 (2006) Abstract
Visfatin, an adipocytokine with proinflammatory and immunomodulating properties: A.R. Moschen, et al.; J. Immunol. 178, 1748 (2007) Abstract
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
NAD+ Metabolism / Related ProductsPolyclonal Antibodies
 
 
ALX-210-426 Revised 23-Oct-08
Polyclonal Antibody to Nampt/Visfatin (human) (NT)
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