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NF-kB Pathway
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APO-54N-039 Revised 10-Oct-08 New product
MDA5 (human) (IntraCellular) ELISA Kit
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SYNONYMS Melanoma Differentiation-associated Gene 5 (human) (IntraCellular) ELISA Kit
Helicard (human) (IntraCellular) ELISA Kit
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
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APO-54N-039-KI01   1 Kit 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 0.4ng/ml (range 0.5 to 32ng/ml).
QUANTITY: For 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human MDA5 in cell extracts.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • ELISA & EIA Kits
 
 
ALX-260-092 Revised 05-Jun-08
MG-132
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SYNONYMS Z-LLL-CHO
Z-Leu-Leu-Leu-CHO
PRODUCT LINE Ubiquitin & Proteasome
PRODUCT CATEGORY Proteasome / Related Products
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ALX-260-092-M001   1 mg 25.00 USD Add To Cart
ALX-260-092-M005   5 mg 60.00 USD Add To Cart
Product Specification
SEQUENCE: Z-Leu-Leu-Leu-CHO
FORMULA: C26H41N3O5
MW: 475.6
CAS NUMBER: 133407-82-6
PURITY: ≥95% (HPLC)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
IDENTITY: Identity determined by MS.
Product Description
Potent, reversible and cell permeable proteasome inhibitor. Inhibits NF-κB activation.
Product Specific Literature References
Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules: K.L. Rock, et al.; Cell 78, 761 (1994) Abstract
Multiple proteolytic systems, including the proteasome, contribute to CFTR processing: T.J. Jensen, et al.; Cell 83, 129 (1995) Abstract
The proteasome pathway is required for cytokine-induced endothelial-leukocyte adhesion molecule expression: M.A. Read, et al.; Immunity 2, 493 (1995) Abstract
Selective inhibitors of the proteasome-dependent and vacuolar pathways of protein degradation in Saccharomyces cerevisiae: D.H. Lee and A.L. Goldberg; J. Biol. Chem. 271, 27280 (1996) Abstract; Full Text
The human cytomegalovirus US11 gene product dislocates MHC class I heavy chains from the endoplasmic reticulum to the cytosol: E.J. Wiertz, et al.; Cell 84, 769 (1996) Abstract
Proteasome inhibitors activate stress kinases and induce Hsp72. Diverse effects on apoptosis: A.B. Meriin, et al.; J. Biol. Chem. 273, 6373 (1998) Abstract; Full Text
Role of proteasomal degradation in the cell cycle-dependent regulation of DNA topoisomerase IIalpha expression: L. Salmena, et al.; Biochem. Pharmacol. 61, 795 (2001) Abstract
Further Categories Containing This Product:
NF-kB Pathway Inhibitors
 
 
ALX-804-137 Revised 07-Dec-05
Monoclonal Antibody to A20 (human) (8E8.38)
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PRODUCT LINE Inflammation
PRODUCT CATEGORY TLRs Other Products
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ALX-804-137-C050   50 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 8E8.38
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Zinc finger domain corresponding to aa 375-537 of human A20.
SPECIFICITY: Recognizes human A20.
APPLICATION: Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
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Product Description
A20 is a zinc finger protein that renders cells resistant to apoptosis. A20-deficient mice develop severe inflammation and are hyper-responsive to LPS. S.M. O'Reilly & P.N. Moynagh have shown that full length A20 inhibits activation of NF-kB and AP-1 by TLR4 [1].  A20 associates with A20-binding inhibitor of NF-kB (ABIN)-1 and -2 [2-4], which interfere with NF-kB activation upstream of the IKK complex.
Product Specific Literature References
A20 inhibits NF-kappa B activation downstream of multiple Map3 kinases and interacts with the I kappa B signalosome: F.S. Zetoune, et al.; Cytokine 15, 282 (2001) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Regulation of Toll-like receptor 4 signalling by A20 zinc finger protein: S.M. O'Reilly and P.N. Moynagh; Biochem. Biophys. Res. Commun. 3303, 586 (2003) Abstract
[2] The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN: K. Heyninck, et al.; J. Cell Biol. 145, 1471 (1999) Abstract; Full Text
[3] Identification of a novel A20-binding inhibitor of nuclear factor-kappa B activation termed ABIN-2: S. Van Huffel, et al.; J Biol Chem 276, 30216 (2001) Abstract; Full Text
[4] Structure-function analysis of the A20-binding inhibitor of NF-kappa B activation, ABIN-1: K. Heyninck, et al.; FEBS Lett. 536, 135 (2003) Abstract
Further Categories Containing This Product:
NF-kB Pathway Other Products • Monoclonal Antibodies • Cell Death / Apoptosis / Autophagy Other Products
 
 
ALX-804-134 Revised 01-Mar-07
Monoclonal Antibody to Bcl-10 (Carmen-1)
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SYNONYMS anti-CARMEN MAb (Carmen-1)
anti-CIPER MAb (Carmen-1)
anti-CLAP MAb (Carmen-1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Caspase Recruitment Domain [CARD] Proteins / Related Products
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ALX-804-134-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Carmen-1
ISOTYPE: Rat IgM
SOURCE/HOST: Purified from concentrated hybridoma cell culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE)
PURITY DETAIL: Purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Bcl-10.
SPECIFICITY: Recognizes human and mouse Bcl-10.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY:

Stable for at least 6 months after receipt when stored undiluted at +4°C.
HANDLING: Do not freeze/thaw.
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
NF-kB Pathway Other Products • Innate Immunity Other Products • Monoclonal Antibodies
 
 
ALX-804-847 Revised 19-Jun-08
Monoclonal Antibody to Cardif (human) (Adri-1)
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SYNONYMS anti-CARD Adapter Inducing Interferon-β (human) MAb (Adri-1)
anti-IPS-1 Cardif (human) MAb (Adri-1)
anti-Interferon-β Promoter Stimulator Protein 1 (human) MAb (Adri-1)
anti-MAVS (human) MAb (Adri-1)
anti-Mitochondrial Antiviral Signalling Protein (human) MAb (Adri-1)
anti-VISA (human) MAb (Adri-1)
anti-Virus-induced Signalling Adapter (human) MAb (Adri-1)
PRODUCT LINE Immunology
PRODUCT CATEGORY Antiviral Signalling / Related Products
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ALX-804-847-C100   100 µg 370.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Adri-1
ISOTYPE: Mouse IgG2b
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
SPECIFICITY: Recognizes human Cardif.
APPLICATION: Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections)
Immunoprecipitation (1:200)
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
TRADD Protein Is an Essential Component of the RIG-like Helicase Antiviral Pathway: M.C. Michallet, et al.; Immunity 28, 651 (2008) Abstract
General Information
RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons.
Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005) Abstract
CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006) Abstract
Further Categories Containing This Product:
NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Polyclonal Antibodies
 
 
ALX-804-530 Revised 14-Aug-07
Monoclonal Antibody to Caspase-1 (mouse) (1H11)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Antibodies
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ALX-804-530-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: 1H11
ISOTYPE: Rat IgG1
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T206ALEMVKEVKEFAAC220) of mouse caspase-1.
SPECIFICITY: Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION: ELISA
Flow Cytometry
Western Blot
Other applications not tested.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death: V.S. Marsden, et al.; Blood 107, 1872 (2006) Abstract; Full Text
General Information
Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release.
General Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998), Methodology Abstract
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9: L.A. O'Reilly, et al.; Cell Death Differ. 9, 832 (2002), Methodology Abstract
Further Categories Containing This Product:
Inflammasome • Monoclonal Antibodies
 
 
ALX-804-531 Revised 10-Mar-06
Monoclonal Antibody to Caspase-1 (mouse) (4G8)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Antibodies
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ALX-804-531-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: 4G8
ISOTYPE: Rat IgG1
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T206ALEMVKEVKEFAAC220) of mouse caspase-1.
SPECIFICITY: Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION: ELISA
Flow Cytometry
Western Blot
Other applications not tested.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
Product Images
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General Information
Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release.
General Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998), Methodology Abstract
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9: L.A. O'Reilly, et al.; Cell Death Differ. 9, 832 (2002), Methodology Abstract
Further Categories Containing This Product:
Inflammasome • Monoclonal Antibodies
 
 
ALX-804-209 Revised 27-May-08
Monoclonal Antibody to IκBα (6A920)
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SYNONYMS anti-NF-κB Inhibitor α MAb (6A920)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Inhibitors of kappa B [IkB] / Related Products
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ALX-804-209-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 6A920
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G affinity-purified.
FORMULATION: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN: Recombinant human IκBα (aa 32-391).
SPECIFICITY: Recognizes both phosphorylated and non-phosphorylated forms of human and mouse IκBα. Detects a band of ~40kDa by Western blot.
APPLICATION: Immunoprecipitation (1µg/ml)
Western Blot (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
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General Literature References
Two waves of nuclear factor kappaB recruitment to target promoters: S. Saccani, et al.; J. Exp. Med. 193, 1351 (2001) Abstract; Full Text
p38-Dependent marking of inflammatory genes for increased NF-kappa B recruitment: S. Saccani, et al.; Nat. Immunol. 3, 69 (2002) Abstract
Modulation of NF-kappaB activity by exchange of dimers: S. Saccani, et al.; Mol. Cell 11, 1563 (2003) Abstract
PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock: T. Ikezoe, et al.; Mol. Pharmacol. 64, 1521 (2003) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-440 Revised 19-Sep-05
Monoclonal Antibody to IκBα (cleaved) (human) (5D1623)
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SYNONYMS anti-NF-κB Inhibitor α (cleaved) (human) MAb (5D1623)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Inhibitors of kappa B [IkB] / Related Products
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ALX-804-440-C100   100 µg 319.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 5D1623
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to the caspase-3 mediated cleavage site of IκBα (NF-κB Inhibitor α).
SPECIFICITY: Recognizes caspase-3 cleaved human IκBα.
APPLICATION: Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-235