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ALX-804-497
Revised 08-Feb-06
Monoclonal Antibody to CD14 (biG 14)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
CD14 / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-497-C100
100 µg
295.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Dog
Pig
Others
CLONE:
biG 14
ISOTYPE:
Mouse IgG2a
CONCENTRATION:
1mg/ml after reconstitution.
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Lyophilized from PBS. Contains no preservatives.
RECONSTITUTION:
Reconstitute with 100µl distilled water.
IMMUNOGEN:
Monocytes of different species and immunoaffinity purified soluble human CD14.
SPECIFICITY:
Recognizes human (epitope aa 39-44), bovine, pig, dog, horse and guinea pig CD14.
APPLICATION:
ELISA
Flow Cytometry
Western Blot
Functional Application (Inhibits binding of LPS to CD14; 1µg/ml MAb inhibits binding of 0.5µg/ml LPS to CD14).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli
:
F. Stelter, et al.; Eur J Biochem
243
, 100 (1997)
Abstract
General Information
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
BACKGROUND/TECHNICAL INFORMATION
Binding titer at human CD14 transfected CHO-cells is more than 10'000.
MANUFACTURER
Manufactured by Biometec.
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-804-498
Revised 18-Jul-07
Monoclonal Antibody to CD14 (human) (biG 2/RoMo-1)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
CD14 / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-498-C100
100 µg
295.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
biG 2/RoMo-1
ISOTYPE:
Mouse IgG2a
CONCENTRATION:
1mg/ml after reconstitution.
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Lyophilized from PBS. Contains no preservatives.
RECONSTITUTION:
Reconstitute with 100µl distilled water.
IMMUNOGEN:
Human monocytes.
SPECIFICITY:
Recognizes human (epitope aa 147-152) CD14.
APPLICATION:
ELISA
Flow Cytometry
Immunoprecipitation
Note:
Does
not inhibit binding of LPS to CD14.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli:
F. Stelter, et al.; Eur J Biochem
243
, 100 (1997)
Abstract
General Information
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
BACKGROUND/TECHNICAL INFORMATION
Binding titer at human CD14 transfected CHO-cells (FACS) is 1:5000.
MANUFACTURER
Manufactured by Biometec.
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-804-499
Revised 08-Apr-08
Monoclonal Antibody to CD14 (mouse) (biG 53)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
CD14 / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-499-C100
100 µg
490.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
biG 53
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml after reconstitution.
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Lyophilized. Contains no preservatives.
RECONSTITUTION:
Reconstitute with 100µl distilled water.
IMMUNOGEN:
Recombinant mouse CD14.
SPECIFICITY:
Recognizes mouse CD14. Binds to LPS-activated mouse monocytes.
APPLICATION:
ELISA
Flow Cytometry
Functional Application (Inhibits binding of LPS to CD14; up to 1µg/ml CD14 inhibits binding of 0.5µg/ml FITC-LPS to 6x10
5
CD14
+
CHO transfectants (FACS)).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
General Information
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in mouse serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
BACKGROUND/TECHNICAL INFORMATION
Binding titer at mouse CD14 transfected CHO-cells is more than 20'000.
MANUFACTURER
Manufactured by Biometec.
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-805-062
Revised 15-Jan-08
Monoclonal Antibody to CD18 (human) (MEM-48)
SYNONYMS
anti-β2 Integrin (human) MAb (MEM-48)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD11a, CD18 [LFA-1] & CD54 [ICAM-1], CD102 [ICAM-2], CD50 [ICAM-3] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-062-C100
100 µg
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-48
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN:
Leukocytes from a patient suffering from a LGL-type leukemia.
SPECIFICITY:
Recognizes residues 534-546 in cysteine-rich repeat 3 of the human CD18 (β2 integrin). Cross-reacts with all three leukocyte integrins.
APPLICATION:
Flow Cytometry
Immunohistochemistry (frozen sections)
Immunoprecipitation
Western Blot (Recommended dilution: 2µg/ml in buffer containing laurylmaltoside, 2x non-reducing SDS; SDS-PAGE (6% separating gel, 4% stacking gel), positive control: Kg-1a human leukemia cell lysate)
Functional Application (induces high-affinity LFA-1 conformation in leukocytes)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein
:
V. Bazil, et al.; Folia Biol. (Praha)
36
, 41 (1990)
Abstract
The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases:
T. Cinek and V. Horejsi; J. Immunol.
149
, 2262 (1992)
Abstract
Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins:
L. Ottonello, et al.; Blood
93
, 3505 (1999)
Abstract
;
Full Text
Monoclonal Lym-1 antibody-targeted lysis of B lymphoma cells by neutrophils. Evidence for two mechanisms of FcgammaRII-dependent cytolysis:
L. Ottonello, et al.; J. Leukoc. Biol.
68
, 662 (2000)
Abstract
Human leukocytes contain a large pool of free forms of CD18
:
K. Drbal; BBRC
275
, 295 (2000)
Abstract
A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins:
K. Drbal, et al.; Immunobiology
203
, 687 (2001)
Abstract
A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells:
K. Drbal, et al.; Blood
98
, 1561 (2001)
Abstract
Regulation of CD18 expression on neutrophils in response to fluid shear stress:
S. Fukuda and G.W. Schmid-Schonbein; PNAS
100
, 13152 (2003)
Abstract
;
Full Text
Further Categories Containing This Product:
Complement Receptors / Related Products
•
Integrins / Related Products
•
Monoclonal Antibodies
•
CD Antibodies
ALX-805-043
Revised 18-Mar-08
Monoclonal Antibody to CD18 (human) (MEM-148)
SYNONYMS
anti-β2 Integrin (human) MAb (MEM-148)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD11a, CD18 [LFA-1] & CD54 [ICAM-1], CD102 [ICAM-2], CD50 [ICAM-3] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-043-C100
100 µg
225.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-148
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
Liquid. In PBS, pH 7.4, containing 15 mM sodium azide.
IMMUNOGEN:
Human peripheral blood mononuclear cells.
SPECIFICITY:
Recognizes free human CD18 (β2 integrin) not associated with CD11. Does not cross-react with pig CD18.
APPLICATION:
Flow Cytometry
Immunoprecipitation
Western Blot (non-reducing)
Functional Application (induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Human leukocytes contain a large pool of free forms of CD18:
K. Drbal, et al.; BBRC
275
, 295 (2000)
Abstract
A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins:
K. Drbal, et al.; Immunobiology
203
, 687 (2001)
Abstract
A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells:
K. Drbal, et al.; Blood
98
, 1561 (2001)
Abstract
;
Full Text
Further Categories Containing This Product:
Complement Receptors / Related Products
•
Monoclonal Antibodies
•
Integrins / Related Products
•
CD Antibodies
ALX-805-049
Revised 18-Aug-08
Monoclonal Antibody to CD19 (human) (LT19)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-049-C100
100 µg
195.00 USD
ALX-805-049F-T100
FITC
100 tests
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
LT19
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Purified by DEAE-chromatography and precipitation.
FORMULATION:
Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN:
Human B lymphoblast cell line Daudi.
SPECIFICITY:
Recognizes human CD19.
APPLICATION:
Flow Cytometry
Immunoprecipitation
SHIPPING:
SHIPPED ON BLUE ICE
HANDLING:
For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
Product Specific Literature References
Strong cytosine-guanosine-independent immunostimulation in humans and other primates by synthetic oligodeoxynucleotides with PyNTTTTGT motifs:
F. Ellias, et al.; J. Immunol.
171
, 3697 (2003)
Abstract
;
Full Text
CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features:
C.W. Lin, et al.; Blood
106
, 3567 (2005)
Abstract
;
Full Text
Further Categories Containing This Product:
B Cell Receptor [BCR] & Co-Receptors / Related Products
•
Monoclonal Antibodies
ALX-805-047
Revised 10-Nov-04
Monoclonal Antibody to CD20 (human) (MEM-97)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD20 & B Cell Markers / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-047-C100
100 µg
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-97
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. In PBS, pH 7.4, containing BSA and 15mM sodium azide.
IMMUNOGEN:
Burkitt's lymphoma cell line Raji.
SPECIFICITY:
Recognizes human CD20.
APPLICATION:
Flow Cytometry
: Use 20µl reagent for analysis of human blood cells and 100µl of whole blood;
Immunoprecipitation
.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
HANDLING:
MEM-97-FITC: Avoid prolonged exposure to light! DO NOT FREEZE!
Product Specific Literature References
Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY
:
J. Szollosi, et al.; J. Immunol.
157
, 2939 (1996)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-805-050
Revised 11-Feb-04
Monoclonal Antibody to CD21 (human) (LT21)
SYNONYMS
anti-CR2 (human) MAb (LT21)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-050-C100
100 µg
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
LT21
ISOTYPE:
Mouse IgG1
IMMUNOGEN:
Human B lymphoblast cell line IM9.
SPECIFICITY:
Recognizes human CD21 (CR2).
APPLICATION:
Flow Cytometry.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-805-051
Revised 15-Jul-05
Monoclonal Antibody to CD22 (human) (MEM-01)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Immunoglobulin Superfamily Cell Adhesion Molecules [IgSF CAMs] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-051-C100
100 µg
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Monkey
CLONE:
MEM-01
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN:
Burkitt's lymphoma cell line Raji.
SPECIFICITY:
Recognizes human and monkey CD22. Stains only the zone corresponding to 140kDa. Does not cross-block MAb to CD22 (human) (RFB4) (Prod. No.
ANC-171
).
APPLICATION:
Flow Cytometry
Immunoprecipitation
Western Blot (non-reducing)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 6 months when stored at +4°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
B Cell Receptor [BCR] & Co-Receptors / Related Products
•
Monoclonal Antibodies
•
CD Antibodies
ALX-805-060
Revised 22-Jun-05
Monoclonal Antibody to CD25 (human) (MEM-181)
SYNONYMS
anti-IL-2R
α
(human) MAb (MEM-181)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD25 [IL-2Ralpha] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-060-C100
100 µg
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-181
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN:
PHA-activated peripheral blood leukocytes.
SPECIFICITY:
Recognizes human CD25.
APPLICATION:
Flow Cytometry
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 6 months when stored at +4°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-805-003
Revised 23-Nov-05
Monoclonal Antibody to CD31 (human) (Gi18)
SYNONYMS
anti-PECAM-1 (human) MAb (Gi18)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-003A-C100
Purified
100 µg
250.00 USD
ALX-805-003B-T100
Biotin
100 tests
308.00 USD
ALX-805-003F-T100
FITC
100 tests
308.00 USD
