ALEXIS Biochemicals: PARP-1 (bovine) (~90%)

ALX-202-042

Revised 16-Apr-08

PARP-1 (bovine) (~90%)
SYNONYMS	Poly(ADP-ribose) Polymerase-1 (bovine) (~90%)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	PARP-1

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-202-042-C010		10 µg	180.00 USD

Product Specification

EC:	2.4.2.30
SOURCE/HOST:	Isolated from calf thymus.
CONCENTRATION:	335μg/ml
PURITY:	~90% (SDS-PAGE, Western blot)
FORMULATION:	Liquid. In 60mM kalium phosphate, pH 7.2, containing 0.5 - 2M kalium chloride, 10% glycerol, 1mM DTT, 10mM β−mercaptoethanol, 0.1mM 0.1 PMSF.
SPECIFIC ACTIVITY:	500-700U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol poly(ADP-ribose) per min. at 25°C.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Do not freeze/thaw.

Product Specific Literature References

Erasable blot of poly(ADP-ribose) polymerase: S. Desnoyers, et al.; Anal. Biochem. 218, 470 (1994) Abstract
Autoantibodies reacting with poly(ADP-ribose) and with a zinc-finger functional domain of poly(ADP-ribose) polymerase involved in the recognition of damaged DNA: S. Muller, et al.; Clin. Immunol. Immunopathol. 73, 187 (1994) Abstract
Characterization of the execution phase of apoptosis in vitro using extracts from condemned-phase cells: Y.A. Lazebnik, et al.; J. Cell Sci. Suppl. 19, 41 (1995) Abstract
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik, et al.; PNAS 92, 9042 (1995) Abstract; Full Text
Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo: G.M. Shah, et al.; Anal. Biochem. 227, 1 (1995), (Review) Abstract
Detection of poly(ADP-ribose) polymerase and its apoptosis-specific fragment by a nonisotopic activity-western blot technique: G.M. Shah, et al.; Anal. Biochem. 232, 251 (1995) Abstract
C.J. Froelich, et al.; BBRC 227, 658 (1996) Abstract
Proteolysis of poly(ADP-ribose) polymerase by caspase 3: kinetics of cleavage of mono(ADP-ribosyl)ated and DNA-bound substrates: D. D'Amours, et al.; Radiat. Res. 150, 3 (1998) Abstract
Towards new neuroprotective agents: design and synthesis of 4H-thieno[2,3-c] isoquinolin-5-one derivatives as potent PARP-1 inhibitors: R. Pellicciari, et al.; Farmaco 58, 851 (2003) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P18493: PARP-1 (bovine)

Further Categories Containing This Product:

Enzymes • Natural Proteins

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