ALEXIS Biochemicals: PARP-1 (bovine) (~98%)

ALX-202-046

Revised 25-Oct-06

PARP-1 (bovine) (~98%)
SYNONYMS	Poly(ADP-ribose) Polymerase-1 (bovine) (~98%)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	PARP-1

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-202-046-C010		10 µg	250.00 USD

Product Specification

EC:	2.4.2.30
SOURCE/HOST:	Isolated from calf thymus.
CONCENTRATION:	65.2µg/ml
PURITY:	~98% (SDS-PAGE, Western blot)
FORMULATION:	Liquid. In 50mM TRIS-HCl, pH 7.4, containing 1M NaCl, 10mM β-mercaptoethanol and 10mM 3-methoxybenzamide.
SPECIFIC ACTIVITY:	300-400U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol of poly(ADP-ribose) per min. at 25°C.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

[1] Poly(ADP-ribose) polymerase is a zinc metalloenzyme: P. Zahradka & K. Ebisuzaki; Eur. J. Biochem. 142, 503 (1984) Abstract
[2] Erasable blot of poly(ADP-ribose) polymerase: S. Desnoyers, et al.; Anal. Biochem. 218, 470 (1994) Abstract
[3] Autoantibodies reacting with poly(ADP-ribose) and with a zinc-finger functional domain of poly(ADP-ribose) polymerase involved in the recognition of damaged DNA: S. Muller, et al.; Clin. Immunol. Immunopathol. 73, 187 (1994) Abstract
[4] Characterization of the execution phase of apoptosis in vitro using extracts from condemned-phase cells: Y.A. Lazebnik, et al.; J. Cell Sci. Suppl. 19, 41 (1995) Abstract
[5] Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik, et al.; PNAS 92, 9042 (1995) Abstract; Full Text
[6] Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo: G.M. Shah, et al.; Anal. Biochem. 227, 1 (1995), (Review) Abstract
[7] Detection of poly(ADP-ribose) polymerase and its apoptosis-specific fragment by a nonisotopic activity-western blot technique: G.M. Shah, et al.; Anal. Biochem. 232, 251 (1995) Abstract
[8] Granzyme B/perforin-mediated apoptosis of Jurkat cells results in cleavage of poly(ADP-ribose) polymerase to the 89-kDa apoptotic fragment and less abundant 64-kDa fragment: C.J. Froelich, et al.; BBRC 227, 658 (1996) Abstract
[9] Purification of the death substrate poly(ADP-ribose) polymerase: D. D'Amours, et al.; Anal. Biochem. 249, 106 (1997) Abstract
[10] Characterization of the necrotic cleavage of poly(ADP-ribose) polymerase (PARP-1): implication of lysosomal proteases: S. Gobeil, et al.; Cell Death Differ. 8, 588 (2001) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Purified by 3-aminobenzamide affinity chromatography (for method see [9]).

Swiss-Prot link P18493: PARP-1 (bovine)

Further Categories Containing This Product:

Enzymes • Natural Proteins

header picture © micronaut.ch, another CS2 website

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.