ALEXIS Biochemicals: PARP-1 (human) (recombinant) (high purity)

ALX-201-063

Revised 25-Oct-06

PARP-1 (human) (recombinant) (high purity)
SYNONYMS	Poly(ADP-ribose) Polymerase-1 (human) (recombinant) (high purity)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	PARP-1

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-063-C020		20 µg	348.00 USD

Product Specification

EC:	2.4.2.30
SOURCE/HOST:	Produced in Sf9 cells.
CONCENTRATION:	1mg/ml.
PURITY:	≥99% (SDS-PAGE
PURITY DETAIL:	Affinity purified.
FORMULATION:	Liquid. In 100mM TRIS-HCl, pH 7.5,containing 14mM β-mercaptoethanol, 0.5mM EDTA, 0.5mM PMSF and 10% glycerol.
SPECIFIC ACTIVITY:	≥600U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol of poly(ADP-ribose) per min. at 25°C, pH 7.5.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

Product Images

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Product Specific Literature References

Overproduction and large-scale purification of the human poly(ADP- ribose) polymerase using a baculovirus expression system: H. Giner, et al.; Gene 114, 279 (1992) Abstract
Poly(ADP-ribose) reactivates stalled DNA topoisomerase I and Induces DNA strand break resealing: M. Malanga & F.R. Althaus; J. Biol. Chem. 279, 5244 (2004) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P09874: PARP-1 (human)