ALEXIS Biochemicals: PARP-2 (mouse) (recombinant) (high purity)

ALX-201-064

Revised 25-Oct-06

PARP-2 (mouse) (recombinant) (high purity)
SYNONYMS	Poly(ADP-ribose) Polymerase-2 (mouse) (recombinant) (high purity)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	PARP-2

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-064-C020		20 µg	368.00 USD

Product Specification

EC:	2.4.2.30
SOURCE/HOST:	Produced in Sf9 cells.
CONCENTRATION:	~1mg/ml
PURITY:	≥98% (SDS-PAGE)
PURITY DETAIL:	Affinity purified.
FORMULATION:	Liquid. In 100mM TRIS-HCl, pH 7.5, containing 14mM β-mercaptoethanol, 0.5mM EDTA, 0.5mM PMSF and 10% glycerol.
SPECIFIC ACTIVITY:	≥380U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol of poly(ADP-ribose) per min at 25°C, pH 7.5. See also separate protocol (link below).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

Product Images

Please click on thumbnails to enlarge.

Product Specific Literature References

PARP-2, A novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase: J.C. Ame, et al.; J. Biol. Chem. 274, 17860 (1999) Abstract; Full Text
A bidirectional promoter connects the poly(ADP-ribose) polymerase 2 (PARP-2) gene to the gene for RNase P RNA. structure and expression of the mouse PARP-2 gene: J.C. Ame, et al.; J. Biol. Chem. 276, 11092 (2001) Abstract; Full Text
Poly(ADP-ribose) reactivates stalled DNA topoisomerase I and Induces DNA strand break resealing: M. Malanga & F.R. Althaus; J. Biol. Chem. 279, 5244 (2004) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link O88554: PARP-2 (mouse)