ALEXIS Biochemicals: Phorbol 12-myristate 13-acetate

ALX-445-004

Revised 08-Oct-08

Phorbol 12-myristate 13-acetate
SYNONYMS	PMA 12-O-Tetradecanoylphorbol 13-acetate TPA
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Phorbols / Phorbol Esters

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-445-004-M001		1 mg	18.00 USD
ALX-445-004-M005		5 mg	60.00 USD
ALX-445-004-M010		10 mg	110.00 USD
ALX-445-004-M025		25 mg	210.00 USD

Product Specification

FORMULA:	C₃₆H₅₆O₈
MW:	616.8
CAS NUMBER:	16561-29-8
MERCK INDEX:	14: 7332
RTECS:	QH4377000
PURITY:	≥98% (HPLC)
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in DMSO, methanol, 100% ethanol, acetone, ether or dimethylformamide; almost insoluble in aqueous buffers.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Dissolve in an organic solvent prior to dilution into aqueous buffers. Add calculated volume of organic solvent to vial to prepare concentrated stock solution. Intermediate dilutions into the same organic solvent are recommended prior to final dilution into aqueous buffer.
HANDLING:	Protect from light.
HAZARD:	MAY BE CARCINOGENIC. HARMFUL. MAY BE MUTAGENIC.

Product Description

Most commonly used phorbol ester. Binds to and activates protein kinase C (PKC). Causes an extremely wide range of effects in cells and tissues. Very potent mouse skin tumor promoter. Inhibits apoptosis induced by Fas, but induces apoptosis in HL-60 promyelocytic leukemia cells.

Product Specific Literature References

Autoxidation of phorbol esters under normal storage conditions: R. Schmidt & E. Hecker; Cancer Res. 35, 1375 (1975) Abstract
Phorbol esters as signal transducers and tumor promoters: M. Castagna; Biol. Cell 59, 3 (1987), Review Abstract
P.M. Blumberg; CRC Crit. Rev. Toxicol 8, 153 (1988)
Differential irreversible insertion of protein kinase C into phospholipid vesicles by phorbol esters and related activators: M.G. Kazanietz, et al.; J. Biol. Chem. 267, 20878 (1992) Abstract
Phorbol esters induce nitric oxide synthase activity in rat hepatocytes. Antagonism with the induction elicited by lipopolysaccharide: S. Hortelano, et al.; J. Biol. Chem. 267, 24937 (1992) Abstract; Full Text
Phorbol ester induces apoptosis in HL-60 promyelocytic leukemia cells but not in HL-60 PET mutant: D.E. Macfarlane & P.S. O'Donnel; Leukemia 7, 1846 (1993) Abstract
Effect of phorbol 12-myristate 13-acetate on Ca2+-ATPase activity in rat liver nuclei: K. Oishi & M. Yamaguchi; J. Cell. Biochem. 55, 168 (1994) Abstract
Role for ceramide as an endogenous mediator of Fas-induced cytotoxicity: C.G. Tepper, et al.; PNAS 92, 8443 (1995) Abstract
Evidence for different mechanisms of growth inhibition of T-cell lymphoma by phorbol esters and concanavalin A: S. Desrivieres, et al.; J. Biol. Chem. 272, 2470 (1997) Abstract
Phorbol esters and synthetic diacylglycerols inhibit gamma-aminobutyric acid uptake in human spermatozoa: an effect mediated by protein kinase C?: A. Aanesen, et al.; Mol. Hum. Reprod. 4, 339 (1998) Abstract
Gene expression of TPA induced differentiation in HL-60 cells by DNA microarray analysis: X. Zheng, et al.; Nucleic Acids Res. 30, 4489 (2002) Abstract; Full Text
Phorbol esters and neurotransmitter release: more than just protein kinase C?: E.M. Silinsky and T.J. Searl; Br. J. Pharmacol. 138, 1191 (2003), Review Abstract
Characterization of phorbol esters activity on individual mammalian protein kinase C isoforms, using the yeast phenotypic assay: L. Saraiva, et al.; Eur. J. Pharmacol. 491, 101 (2004) Abstract

Further Categories Containing This Product:

PKC Activators • Natural Products - Apoptosis Inducers & Inhibitors

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