ALEXIS Biochemicals: Polyclonal Antibody to CCR8 (human) (NT)

ALX-210-762

Revised 25-Jan-07

Polyclonal Antibody to CCR8 (human) (NT)
SYNONYMS	anti-C-C Chemokine Receptor 8 (human) (NT) PAb anti-CD198 (human) (NT) PAb
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	CC Chemokines & Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-762-C200		200 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From goat.
CONCENTRATION:	2mg/ml
PURITY DETAIL:	Epitope-affinity purified IgG.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 12-36 (V¹²TDYYYPDIFSSPCDAELIQTNGKL³⁶) of the N-terminal extracellular domain of human CCR8 (C-C chemokine receptor 8).
SPECIFICITY:	Recognizes human CCR8.
APPLICATION:	Flow Cytometry (2-5µl per 10⁶cells/50-100µl) Immunocytochemistry (acetone fixed cells (1:800)) Immunohistochemistry (paraffin sections (1:250)) Western Blot (1:2’000) Functional Application (neutralizing; inhibits Lp(a) induced MCA in HUVECS)
QUALITY CONTROL:	Immunocytochemistry on human K562 cells and immunohistochemistry on human spleen tissue slices.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -20°C.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-153-024.

Product Specific Literature References

The CC chemokine I-309 inhibits CCR8-dependent infection by diverse HIV-1 strains: R. Horuk, et al.; J. Biol. Chem. 273, 386 (1998) Abstract; Full Text
Molecular cloning and chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and polymorphonuclear cells and encoding a putative chemokine receptor: M. Samson, et al.; Eur. J. Immunol. 26, 3021 (1996) Abstract
CC Chemokine I-309 is the Principal Monocyte Chemottractant Induced by Apolipoprotein(a) in Human Vascular Endothelial Cells: N.S. Haque; Circulation 102, 786 (2000) Abstract
The chemokine receptor CCR8 mediates human endothelial cell chemotaxis induced by I-309 and Kaposi sarcoma herpesvirus-encoded vMIP-I and by lipoprotein(a)-stimulated endothelial cell conditioned medium: N.S. Haque; Blood 97, 39 (2001) Abstract
CCL16 activates an angiogenic program in vascular endothelial cells: M. Strasly, et al.; Blood 103, 40 (2004) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Immunohistochemistry on paraffin sections:
Fix paraffin sections in 10% formalin. De-wax sections and block endogenous peroxide by incubating sections with 3% H2O2 for 15 min. at room temperature (RT). Wash sections with PBS and incubate with 2% normal rabbit serum at room temperature to block non-specific binding sites. Wash sections with PBS and incubate with primary antibody (anti-chemokine receptor) at recommended dilution for 60 min. at RT. Wash slides and incubate at RT with biotinylated rabbit anti-goat IgG H+L (Vector Labs) at 1:200 dilution for 30 min. Wash slides and incubate with HRP-Streptavidin (Vector Labs) 1:400 dilution for 30 min. Wash slides and incubate with DAB for 5-7 min. at RT. Wash slides with PBS and counter stain with hematoxylin.

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Polyclonal Antibodies

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