ALEXIS Biochemicals: Polyclonal Antibody to Calsequestrin

ALX-210-290

Revised 11-May-07

Polyclonal Antibody to Calsequestrin
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calcium Signalling (Intracellular) Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-290-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Sheep Dog
SOURCE/HOST:	From rabbit.
FORMULATION:	Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:	Purified dog cardiac calsequestrin.
SPECIFICITY:	Recognizes human, mouse, rat, dog and sheep calsequestrin. Recognizes both cardiac and skeletal muscle calsequestrin. Detects a band of ~55kDa by Western blot.
CROSSREACTIVITY PROTEINS:	Additional bands at ~97kDa may be observed and have been reported to be calsequestrin-like proteins.
APPLICATION:	Immunoprecipitation Western Blot (1:2'500)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Developmental changes in cardiac sarcoplasmic reticulum in sheep: L. Mahony & L.R. Jones; J. Biol. Chem. 261, 15257 (1986) Abstract; Full Text
Complex formation between junctin, triadin, calsequestrin, and the ryanodine receptor. Proteins of the cardiac junctional sarcoplasmic reticulum membrane: L. Zhang, et al.; J. Biol. Chem. 272, 23389 (1997) Abstract; Full Text
Identification of triadin 1 as the predominant triadin isoform expressed in mammalian myocardium: Y.M. Kobayashi & L.R. Jones; J. Biol. Chem. 274, 28660 (1999) Abstract; Full Text

General Information

The sarcoplasmic reticulum (SR) is in part responsible for maintaining the level of intracellular Ca²⁺ in cardiac and skeletal muscle by storing and releasing Ca²⁺. Several intralumenal SR Ca²⁺ binding proteins have been identified, the most prominent of these is calsequestrin. Calsequestrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize Ca²⁺ near the junctional face of the terminal cisternae from which Ca²⁺ can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for Ca²⁺. Two mammalian isoforms of calequestrin exist, a cardiac and a skeletal muscle isoform, which are the products of different genes and are 63% identical. This antibody recognizes both cardiac and skeletal muscle calsequestrin from a variety of species.

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Polyclonal Antibodies

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