ALEXIS Biochemicals: Polyclonal Antibody to PARP-1

ALX-210-221

Revised 07-Dec-06

Polyclonal Antibody to PARP-1
SYNONYMS	anti-Poly(ADP-ribose) Polymerase-1 PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	PARP-1

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-221-R100		100 µl	240.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Monkey Others
SOURCE/HOST:	From rabbit.
FORMULATION:	Liquid. Contains 0.02% sodium azide.
IMMUNOGEN:	Purified bovine PARP-1 (poly(ADP-ribose) polymerase-1).
SPECIFICITY:	Recognizes human, mouse, rat, monkey and bovine PARP-1.
APPLICATION:	Immunocytochemistry: 1:500. Dilution buffer: 50mM TRIS, pH 7.5, 150mM NaCl, 5% milk. Immunoprecipitation: 1:50. Dilution buffer: 175mM sodium phosphate, pH 7.5, 100mM NaCl, 1% Triton X100, 0.5% sodium deoxycholate, 0.1% SDS, 0.1mM EDTA and protease inhibitors (0.17µg/ml PMSF, 27µg/ml aprotinin, 0.5µg/ml leupeptin, 0.5µg/ml pepstatin, 0.5µg/ml chymostatin, 0.5µg/ml tosylphenylalanine) [2]. Western Blot: 1:5'000. See also separate Western blot protocol. Functional Application: Neutralizes the biological activity of PARP-1.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

Product Specific Literature References

[1] Poly(ADP-ribose) polymerase is a zinc metalloenzyme: P. Zahradka & P. Ebisuzaki; Eur. J. Biochem. 142, 503 (1984) Abstract
[2] Dual function for poly(ADP-ribose) synthesis in response to DNA strand breakage: M.S. Satoh, et al.; Biochemistry 33, 7099 (1994) Abstract
[3] Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo: G.M. Shah, et al.; Anal. Biochem. 227, 1 (1995), (Review) Abstract

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Polyclonal Antibodies

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