ALEXIS Biochemicals: Polyclonal Antibody to PIDD (AL233)

ALX-210-920

Revised 06-Nov-07

Polyclonal Antibody to PIDD (AL233)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Adaptor Proteins for Death Receptors Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-920-C050		50 µg	194.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml
PURITY DETAIL:	Epitope-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human PIDD (Death Domain: aa 776-910).
SPECIFICITY:	Recognizes human and the native form of mouse PIDD. Detects bands of ~90kDa (full length protein) and ~50kDa (cleaved protein) by Western blot.
APPLICATION:	Immunohistochemistry (paraffin sections) Immunoprecipitation (1:100) Western Blot (1:500)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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Product Specific Literature References

The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress: A. Tinel and J. Tschopp; Science 304, 843 (2004) Abstract
PIDD mediates NF-kB activation in Response to DNA damage: Janssens et al.; Cell 123, 1079 (2005) Abstract
Autoproteolysis of PIDD marks the bifurcation between pro-death caspase-2 and pro-survival NF-kappaB pathway: A. Tinel, et al.; EMBO J. 26, 197 (2007) Abstract
Heat shock induces apoptosis independently of any known initiator caspase-activating complex: R.S. Milleron and S.B. Bratton; J. Biol. Chem. 281, 16991 (2006) Abstract; Full Text

General Information

The death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD. PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Pidd, a new death-domain-containing protein, is induced by p53 and promotes apoptosis: Y. Lin, et al.; Nat. Genet. 26, 122 (2000) Abstract

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