ALEXIS Biochemicals: Polyclonal Antibody to PTX3

ALX-210-365

Revised 06-Nov-07

Polyclonal Antibody to PTX3
SYNONYMS	anti-Pentraxin 3 PAb anti-TSG14 PAb anti-TNF Stimulated Gene-14 PAb
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	Pentraxin3 [PTX3]/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-365-C050		50 µg	370.00 USD
ALX-210-365B-C050	Biotin	50 µg	470.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml
PURITY DETAIL:	Affinity purified.
FORMULATION:	Liquid. In PBS containing 0.1% sodium azide.
IMMUNOGEN:	Recombinant human PTX3.
SPECIFICITY:	Recognizes human, mouse, rat and bovine (weak) PTX3 and chicken homolog PPTX.
APPLICATION:	ELISA (detection - excellent): 1:20’000. Use as detection antibody in an ELISA format together with MAb to PTX3 (Prod. No. ALX-804-464 (capture)) and PTX3 standard (Prod. No. ALX-201-124). Immunocytochemistry Immunohistochemistry Immunoprecipitation
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Once thawed store biotinylated antibody at 4°C and do not re-freeze.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Inducible expression of PTX3, a new member of the pentraxin family, in human mononuclear phagocytes: V.V. Alles, et al.; Blood 84, 3483 (1994) Abstract; Full Text
Expression of a long pentraxin, PTX3, by monocytes exposed to the mycobacterial cell wall component lipoarabinomannan: V. Vouret-Craviari; Infect. and Immunity 65, 1345 (1997) Abstract
PTX3, A prototypical long pentraxin, is an early indicator of acute myocardial infarction in humans: G. Peri, et al.; Circulation 102, 636 (2000) Abstract; Full Text
The long pentraxin Ptx3 is synthesized in IgA glomerulonephritis and activates mesangial cells: B. Bussolati, et al.; J. Immunol. 170, 1466 (2003) Abstract
A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel: D.E. Duzhyy, et al.; J. Biol. Chem. 280, 15165 (2005) Abstract
Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease: M. Boehme, et al.; Nephrol. Dial. Transplant. 22, 2224 (2007) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Sandwich ELISA for PTX3

- 96 well-ELISA plates (Nunc MaxiSorp 446612) were coated with 100µl of MAb to PTX3 (human) (MNB4) (Prod. No. ALX-804-464), 700ng/ml in 100µl coating buffer (15mM carbonate buffer pH 9.6) and incubated overnight at 4°C.
- After incubation, the plates were washed three times with 300µl/well of washing buffer (PBS + 0.05% Tween 20), and then 300µl of 5% dry milk in washing buffer were added to block non-specific binding.
- The plates were incubated for 2 hours at room temperature and then washed three times with washing buffer.
- 50µl (in duplicate) recombinant human PTX3 standard (Prod. No. ALX-201-124) (75pg/ml to 2.4ng/ml ), cell supernatants or biological fluids diluted in RPMI 1640 + 2% BSA were added and then incubated for 2 hours at 37°C.
- The plates were washed five times with washing buffer, and then 100µl of PAb to PTX3 (human) (Biotin) (Prod. No. ALX-210-365B) 25ng/ml in washing buffer were added in each well.
- The plates were incubated for 1 hour at 37°C and then washed five times with 300µl of washing buffer.
- 100µl/well streptavidin-horseradish peroxidase diluted 1:4'000 were added and the plates incubated for 1 hour at room temperature.
- After incubation the plates were washed 5 times and 100µl of chromogen substrate ABTS were added. Plates were read after 15 minutes at 405nm in an automatic ELISA reader.