ALEXIS Biochemicals: Polyclonal Antibody to Profilin

ALX-210-740

Revised 29-Mar-07

Polyclonal Antibody to Profilin
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	Actin/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-740-R100		100 µl	295.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Pig Others
SOURCE/HOST:	From rabbit.
CONCENTRATION:	100µg/ml
PURITY DETAIL:	Epitope-affinity purified.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide.
IMMUNOGEN:	Human and pig platelet profilin.
SPECIFICITY:	Recognizes human, mouse, rat, pig and bovine profilin I and II.
APPLICATION:	Western Blot (0.1-0.2µg/ml) Given dilutions refer to the analysis of human cells and tissues with intermediate levels of profilin expression and must be viewed as approximate. Note: Not suitable for immunoprecipitation.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.
POSITIVE CONTROL:	Human platelet protein (500µg) (Prod. No. ALX-840-007), supplied at 5mg/ml in SDS sample buffer (100mM NaCl, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10µg/ml Bromophenol Blue sodium salt). Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.

Product Specific Literature References

A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems: T. Giesemann, et al.; J. Biol. Chem. 274, 37908 (1999) Abstract; Full Text
Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice: W. Hauser, et al.; PNAS 96, 8120 (1999) Abstract; Full Text
Coordinate induction of the actin cytoskeletal regulatory proteins gelsolin, vasodilator-stimulated phosphoprotein, and profilin during capillary morphogenesis in vitro: R. Salazar, et al.; Exp. Cell. Res. 249, 22 (1999) Abstract

General Information

Profilin is a ubiquitous small (12-15kDa) phosphoinositide and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.
Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion, and possibly also in linking the actin cytoskeleton and endocytosis.
Profilin has been found to associate with defined complexes, containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK2 and HEM2/NAP1. The profilin poly-L-proline binding site is primarily thought to anchor profilin at discrete subcellular sites. Ligands of this site include the focal adhesion proteins VASP and Mena, N-WASP, Spinal Muscular Atrophy Protein SMN41, and formin-related proteins like the mammalian Diaphanous homologue p140mDia, Drosophila Cappuchino, S. pombe Cdc12p, as well as S. cerevisiae Bni1p and Bnr1p. In addition, dynamin I and annexin I have also been characterized as profilin binding proteins.

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