ALEXIS Biochemicals: Polyclonal Antibody to VASP (affinity purified)

ALX-210-880

Revised 20-May-08

Polyclonal Antibody to VASP (affinity purified)
SYNONYMS	anti-Vasodilator Stimulated Phosphoprotein (affinity purified)
PRODUCT LINE	Nitric Oxide Pathway
PRODUCT CATEGORY	VASP / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-880-C025		25 µg	390.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Pig
SOURCE/HOST:	From rabbit.
CONCENTRATION:	250µg/ml.
PURITY DETAIL:	Affinity purified.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide.
IMMUNOGEN:	Recombinant human VASP.
SPECIFICITY:	Recognizes human, mouse and pig VASP. Detects both the 46kDa (Ser¹⁵⁷ dephospho) and 50kDa (Ser¹⁵⁷ phospho) forms of VASP.
APPLICATION:	Immunocytochemistry (formaldehyde fixed cells; 1µg/ml) Immunoprecipitation (2µg/ml) Western Blot (0.1µg/ml) Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
POSITIVE CONTROL:	Human platelet protein (500µg) (Prod. No. ALX-840-007), supplied at 5mg/ml in SDS sample buffer (100mM NaCl, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10µg/ml Bromophenol Blue sodium salt). Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.

General Information

VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization:
a) the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a novel proline-rich motif
b) a more divergent proline-rich central domain (which is responsible for profilin binding)
c) a conserved C-terminal EVH2 domain.

VASP is expressed in a variety of mammalian cell types and tissues. In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions. From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp. surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens.

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Polyclonal Antibodies

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