ALEXIS Biochemicals: Polyclonal Antibody to p51A

ALX-210-768

Revised 11-Apr-06

Polyclonal Antibody to p51A
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	p53 Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-768-C200		200 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	2mg/ml
PURITY DETAIL:	Epitope-affinity purified IgG.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 424-446 (E⁴²⁴PRRETPKQSDVFFRHSKPPNRS⁴⁴⁶) of C-terminal human p51A.
SPECIFICITY:	Recognizes human and mouse p51A. Detects a band of ~54kDa by Western blot. Does not cross-react with p51B.
APPLICATION:	Immunocytochemistry (1:300) Immunohistochemistry (paraffin sections) Western Blot (1:1'000)
QUALITY CONTROL:	Western blot on mouse thymus, mouse heart and human T cell line Jurkat.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-165-040.

General Information

p51 is a member of the p53 tumor suppressor gene family, which is induced by DNA damage and/or stress stimuli, causing cells to undergo G1-arrest or apoptotic death. Unlike ubiquitous expression of p53, expression of p51 is restricted to a limited number of tissues. The p51 gene encodes two major splicing variants, p51A (448 aa protein with a molecular weight of 50.9kDa) and p51B (641 aa protein with a molecular weight of 71.9kDa), a human homolog of rat Ket. p51A and p51B differ in their carboxyl-terminal structure.

BACKGROUND/TECHNICAL INFORMATION

Most of the p51A protein in Jurkat and K562 tumor cell lines is degraded.

General Literature References

Cloning and functional analysis of human p51, which structurally and functionally resembles p53: M. Osada, et al.; Nature Med. 4, 839 (1998) Abstract
Human p53-p51 (p53-related) fusion protein: a potent BAX transactivator: S. Ikawa, et al.; Jpn. J. Cancer Res. 90, 596 (1999) Abstract
p53 family genes: structural comparison, expression and mutation: S. Ikawa, et al.; Cell Death Differ. 6, 1154 (1999) Abstract
p53 is a transcriptional activator of the muscle-specific phosphoglycerate mutase gene and contributes in vivo to the control of its cardiac expression: P. Ruiz-Lozano, et al.; Cell Growth Differ. 10, 295 (1999) Abstract; Full Text
Failure of viral oncoproteins to target the p53-homologue p51A: J. Roth & M. Dobbelstein; J. Gen. Virol. 80, 3251 (1999) Abstract; Full Text
Mutational analysis of p51A/TAp63gamma, a p53 homolog, in non-small cell lung cancer and breast cancer: M. Sunahara, et al.; Oncogene 18, 3761 (1999) Abstract

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Polyclonal Antibodies

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