ALEXIS Biochemicals: Polyclonal Antibody to MCAD

ALX-210-261

Revised 25-Jun-08

Polyclonal Antibody to MCAD
SYNONYMS	anti-Medium-chain Fatty Acyl-CoA Dehydrogenase PAb
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	PPAR Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-261-1		1 Vial	313.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Sheep Pig
SOURCE/HOST:	From rabbit.
CONCENTRATION:	0.6mg/ml
PURITY DETAIL:	Affinity purified.
FORMULATION:	Liquid. In 500μl TBS, pH 7.4, containing 50% glycerol, 0.5mg/ml BSA and 0.02% sodium azide.
IMMUNOGEN:	Recombinant human MCAD (medium-chain fatty acyl-CoA dehydrogenase).
SPECIFICITY:	Recognizes human, mouse, pig and sheep MCAD. Detects a band of ~43kDa by Western blot.
APPLICATION:	Western Blot (1:200)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C

Product Specific Literature References

The tissue-specific expression and developmental regulation of two nuclear genes encoding rat mitochondrial proteins. Medium chain acyl-CoA dehydrogenase and mitochondrial malate dehydrogenase: D.P. Kelly, et al.; J. Biol. Chem. 264, 18921 (1989) Abstract; Full Text
Fatty acid oxidation enzyme gene expression is downregulated in the failing heart: M.N. Sack, et al.; Circulation 94, 2837 (1996) Abstract; Full Text
A role for estrogen-related receptor alpha in the control of mitochondrial fatty acid beta-oxidation during brown adipocyte differentiation: R.B. Vega & D.P. Kelly; J. Biol. Chem. 272, 31693 (1997) Abstract; Full Text
A role for Sp and nuclear receptor transcription factors in a cardiac hypertrophic growth program: M.N. Sack, et al.; PNAS 94, 6438 (1997) Abstract; Full Text

General Information

Medium-chain fatty acyl-CoA dehydrogenase (MCAD) is a mitochondrial enzyme that catalyzes the first step in the β-oxidation of fatty acids. MCAD expression is induced during periods of fasting, when reliance on fatty acids for energy is increased [1,2]. The promoter for MCAD contains a peroxisome proliferator response element (PPRE) and is regulated transcriptionally by peroxisome proliferator-activated receptor alpha (PPARα), a ligand-activated transcription factor involved in the regulation of lipid homeostasis [1,3,4]. Because of this, MCAD expression can be used as a marker to evaluate the in vivo activity of PPARα [1,2]. Human MCAD is approximately 87% homologous to pig and rat MCAD, respectively [5, 6]. MCAD is expressed in liver, heart, kidney, and skeletal muscle.

General Literature References

[1] The peroxisome proliferator-activated receptor regulates mitochondrial fatty acid oxidative enzyme gene expression: T. Gulick, et al.; PNAS 91, 11012 (1994) Abstract; Full Text
[2] A critical role for the peroxisome proliferator-activated receptor alpha (PPARalpha) in the cellular fasting response: the PPARalpha-null mouse as a model of fatty acid oxidation disorders: T.C. Leone, et al.; PNAS 96, 7473 (1999) Abstract; Full Text
[3] A pleiotropic element in the medium-chain acyl coenzyme A dehydrogenase gene promoter mediates transcriptional regulation by multiple nuclear receptor transcription factors and defines novel receptor-DNA binding motifs: M.E. Carter, et al.; Mol. Cell. Biol. 14, 4360 (1994) Abstract
[4] Peroxisome proliferator-activated receptors: a nuclear receptor signaling pathway in lipid physiology: T. Lemberger, et al.; Annu. Rev. Cell Dev. Biol. 12, 335 (1996), (Review) Abstract
[5] Nucleotide sequence of medium-chain acyl-CoA dehydrogenase mRNA and its expression in enzyme-deficient human tissue: D.P. Kelly, et al.; PNAS 84, 4068 (1987) Abstract
[6] Molecular cloning and nucleotide sequence of cDNA encoding the entire precursor of rat liver medium chain acyl coenzyme A dehydrogenase: Y. Matsubara, et al.; J. Biol. Chem. 262, 10104 (1987) Abstract

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