ALEXIS Biochemicals: Polyclonal Antibody to Poly(ADP-ribose) (96-10-04)

ALX-210-890

Revised 12-Mar-08

Polyclonal Antibody to Poly(ADP-ribose) (96-10-04)
SYNONYMS	anti-PAR PAb (96-10-04)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Poly(ADP-ribose) [PAR]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-890-R100		100 µl	245.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Others
SOURCE/HOST:	From rabbit.
FORMULATION:	Liquid. Contains 0.02% sodium azide.
IMMUNOGEN:	Poly(ADP-ribose) with methylated BSA.
SPECIFICITY:	Recognizes poly(ADP-ribose) of 6-100 bases in size, synthesized in vitro or in vivo. Shows some cross-reactivity with BSA.
CROSSREACTIVITY PROTEINS:	BSA
APPLICATION:	ELISA (1:10’000) Buffer: PBS, pH 7.4, 1% non fat dried milk and 0.05% Tween 20. Detectable Antigen Range: Titer: 1:125’000 (OD ~0.2), Antigen: 50ng of poly(ADP-ribose). Immunocytochemistry (1:200) Immunoprecipitation 1µl antibody/5µl magnetic beads in a lysis buffer containing 150mM KPO4, pH 8.0, containing 1% NP-40 and 150mM sodium chloride. Western Blot (1:5’000) Buffer: PBS, pH 7.4, 5% non fat dried milk and 0.1% Tween 20. Antigen Amount: 1-10ng of poly(ADP-ribose). Detection Method: Chemiluminescence ("Renaissance" from Dupont) with a secondary antibody (anti-rabbit IgG) conjugated to peroxidase.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for 6 months when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

Product Specific Literature References

Failure to degrade poly(ADP-ribose) causes increased sensitivity to cytotoxicity and early embryonic lethality: D.W. Koh, et al.; PNAS 101, 17699 (2004) Abstract; Full Text
Apoptosis-inducing factor substitutes for caspase executioners in NMDA-triggered excitotoxic neuronal death: H. Wang, et al.; J. Neurosci. 24, 10963 (2004) Abstract; Full Text
MNNG-induced cell death is controlled by interactions between PARP-1, poly(ADP-ribose) glycohydrolase, and XRCC1: C. Keil, et al.; J. Biol. Chem. 281, 34394 (2006) Abstract; Full Text
Ataxia telangiectasia mutated (ATM) signaling network is modulated by a novel poly(ADP-ribose)-dependent pathway in the early response to DNA-damaging agents: J.F. Haince, et al.; J. Biol. Chem. 282, 16441 (2007) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Replaces PAb to Poly(ADP-ribose) (LP98-10) (Prod. No. ALX-210-363) which is no longer available.