ALEXIS Biochemicals: Ponasterone A

ALX-370-014

Revised 16-May-08

Ponasterone A
SYNONYMS	25-Deoxy-20-hydroxyecdysone 25-Deoxyecdysterone 2β,3β,14α,20R,22R-Pentahydroxy-5β-cholest-7-en-6-one
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Ecdysteroids

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-370-014-M001		1 mg	45.00 USD
ALX-370-014-M005		5 mg	180.00 USD
ALX-370-014-M100		100 mg	2'800.00 USD

Product Specification

FORMULA:	C₂₇H₄₄O₆
MW:	464.6
CAS NUMBER:	13408-56-5
MERCK INDEX:	14: 7588
SOURCE/HOST:	Synthetic.
PURITY:	≥95% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in acetic acid, 100% ethanol, methanol or DMSO; insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	To prepare a 1mM stock solution (1.1mM) in 100% ethanol: Add 2ml of 100% ethanol to 1mg (2µmol). Gently mix to dissolve the product (5 to 10 min.). Do not heat solution to dissolve the product. Store the 1mM stock solution at +4°C or at -20°C.

Product Description

A member of the ecdysteroid family. Analog of ecdysone (Prod. No. ALX-370-011) with similar properties to muristerone A (Prod. No. ALX-370-010). It is a functional, reliable and economical substitute for muristerone A as an inducer for the ecdysone-inducible mammalian expression system. Results show that ponasterone A was able to induce expression of β-galactosidase to levels similar to those obtained with muristerone A induction.

Product Specific Literature References

K. Nakanishi, et al.; Chem. Commun. 915 (1966)
H. Moriyama, et al.; THL 9, 1111 (1968)
K. Nakanishi, et al.; Bull. Soc. Chim. France 3475 (1969), (Review)
The uptake and release of ponasterone A by the Kc cell line of Drosophila melanogaster: C. Beckers, et al.; Mol. Cell. Endocrinol. 17, 51 (1980) Abstract
Past and present studies with ponasterones, the first insect molting hormones from plants: K. Nakanishi; Steroids 57, 649 (1992) Abstract
Identification of ligands and coligands for the ecdysone-regulated gene switch: E. Saez, et al.; PNAS 97, 14512 (2000) Abstract
Recent advances in inducible expression in transgenic mice: C. Albanese, et al.; Semin. Cell. Dev. Biol. 13, 129 (2002) Abstract
Development of a ponasterone A-inducible gene expression system for application in cultured skeletal muscle cells: Y.Y. Xiao, et al.; Int. J. Biochem. Cell. Biol. 35, 79 (2003) Abstract

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