ALEXIS Biochemicals: Propidium Iodide

ALX-400-043

Revised 09-May-07

Propidium Iodide
PRODUCT LINE	Other Products
PRODUCT CATEGORY	Dyes / Stains / Fluorescent Probes / Fluorescent Labels

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-400-043-M001		1 mg	77.00 USD

Product Specification

FORMULA:	C₂₇H₃₄N₄I₂
MW:	668.4
CAS NUMBER:	25535-16-4
RTECS:	SF7949600
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. Solution in PBS.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Protect from light.

Product Description

DNA intercalator. Useful for differentiation between apoptotic cell death if used in combination with annexin V conjugates by flow cytometry. Ex(max): 536nm; Em(max): 617nm.

Product Specific Literature References

Propidium iodide staining correlates with the extent of DNA degradation in isolated nuclei: T. Crompton, et al.; BBRC 183, 532 (1992) Abstract
A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells: F. Belloc, et al.; Cytometry 17, 59 (1994) Abstract
Assessment of secondary necrosis of Jurkat cells using a new microscopic system and double staining method with annexin V and propidium iodide: O. Honda, et al.; Int. J. Oncol. 16, 283 (2000) Abstract
Flow cytometric analysis of apoptotic subpopulations with a combination of annexin V-FITC, propidium iodide, and SYTO 17: M. Eray, et al.; Cytometry 43, 134 (2001) Abstract
A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth. 184, 39 (1995) Abstract
Distribution of the human intracellular serpin protease inhibitor 8 in human tissues: M.C. Strik, et al.; J. Histochem. Cytochem. 50, 1443 (2002) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Dilute to 250µg/ml in PBS before use. Add 1µl per annexin V assay.

ALX-620-050	Bisbenzimide H33342 . trihydrochloride
ALX-209-256	Annexin V (human) (recombinant) (FITC)
ALX-209-251	Annexin V (human) (recombinant) (R-PE)
ALX-209-250	Annexin V (human) (recombinant) (FITC)