ALEXIS Biochemicals: RANKL, Soluble (human) (recombinant)

ALX-522-012

Revised 28-Apr-06

RANKL, Soluble (human) (recombinant)
SYNONYMS	ODF, Soluble (human) (recombinant) OPGL, Soluble (human) (recombinant) TRANCE, Soluble (human) (recombinant) TNFSF 11, Soluble (human) (recombinant) CD254, Soluble (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	RANK & RANKL

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-012-C010		10 µg	365.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	~18kDa (non-glycosylated), ~28kDa (glycosylated) (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human RANKL (aa 151-316) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
SOLUBILITY:	Soluble in water.
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds to human and mouse RANK.
BIOLOGICAL ACTIVITY:	Supports the survival of dendritic cells and osteoclasts.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

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Product Specific Literature References

Receptor activator of NF-κB and osteoprotegerin expression by human microvascular endothelial cells, regulation by inflammatory cytokines, and role in human osteoclastogenesis: P. Collin-Osdoby, et al.; J. Biol. Chem. 276, 20659 (2001) Abstract; Full Text
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001) Abstract; Full Text
Suppression of IL-12 Production by Soluble CD40 Ligand: Evidence for Involvement of the p44/42 Mitogen-Activated Protein Kinase Pathway: M. Wittmann, et al.; J. Immunol. 168, 3793 (2002) Abstract
Induction of Cell Cycle Arrest and Apoptosis by the Proteasome Inhibitor PS-341 in Hodgkin Disease Cell Lines Is Independent of Inhibitor of Nuclear Factor-kappaB Mutations or Activation of the CD30, CD40, and RANK Receptors: B. Zheng, et al.; Clin. Cancer Res. 10, 3207 (2004) Abstract
A novel in vivo role for osteoprotegerin ligand in activation of monocyte effector function and inflammatory response: D. Seshasayee, et al.; J. Biol. Chem. 279, 30202 (2004) Abstract

General Information

RANK ligand (RANKL), also called TRANCE, OPGL and ODF, is a member of the TNF family that is expressed in activated T cells, lymph nodes and in stromal cell lines. RANKL (TRANCE) interacts with its receptor RANK expressed on mature dendritic cells (DC) and mature osteoclasts, leading to the inhibition of apoptosis, probably through the upregulation of bcl-x. RANKL is, therefore, a useful tool for enhancing DC and osteoclast survival and activity. Osteoprotegerin (OPG, see Prod. No. ALX-522-007) is a potent inhibitor of RANKL.

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link O014788: RANKL (human)
AfCS Signalling Gateway link A002020: RANKL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function: D.M. Anderson, et al.; Nature 390, 175 (1997) Abstract
TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor: B.R. Wong, et al.; J. Exp. Med. 186, 2075 (1997) Abstract
Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation: D.L. Lacey, et al.; Cell 93, 165 (1998) Abstract
Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL: H. Yasuda, et al.; PNAS 95, 3597 (1998) Abstract

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Recombinant Proteins/Fusion Proteins

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