ALEXIS Biochemicals: Rhodamine 123 . hydrochloride

ALX-610-018

Revised 01-Apr-08

Rhodamine 123 . hydrochloride
SYNONYMS	2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester . chloride
PRODUCT LINE	Other Products
PRODUCT CATEGORY	Dyes / Stains / Fluorescent Probes / Fluorescent Labels

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-610-018-M005		5 mg	20.00 USD

Product Specification

FORMULA:	C₂₁H₁₆N₂O₃ . HCl
MW:	344.4 . 36.5
CAS NUMBER:	62669-70-9
PURITY:	≥96% (HPLC)
APPEARANCE:	Red-brown powder.
SOLUBILITY:	Soluble in 100% ethanol (1mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

A lipophilic cationic fluorescent dye (Ex(max): 507nm; Em(max): 529nm), which selectively locates mitochondria in living cells [1]. Relatively non-toxic [1,2]. Effluxed efficiently by MDR cells [3,4] which is inhibited by established modulators of MDR [4,5]. By following cellular rhodamine 123 accumulation or retention in the presence or absence of P-glycoprotein (Pgp) modulators, gives valuable information on Pgp activity and efficacy of these modulators in reversing Pgp activity can be obtained [6-9]. Since rhodamine 123 is considered a permeant cationic fluorescent probe [2], its uptake into cells has generally been assumed to occur via a passive diffusion process [10]. Recently it was suggested that performing rhodamine 123 accumulation studies without first ascertaining a possible role for a carrier system for cellular uptake of rhodamine 123 and putative Pgp modulators might inadvertently lead to draw improper conclusions on Pgp activity [11].

Product Specific Literature References

[1] Localization of mitochondria in living cells with rhodamine 123: L.V. Johnson, et al.; PNAS 77, 990 (1980) Abstract
[2] Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy: L.V. Johnson, et al.; J. Cell. Biol. 88, 526 (1981) Abstract
[3] Cross-resistance to rhodamine 123 in Adriamycin- and daunorubicin- resistant Friend leukemia cell variants: H. Tapiero, et al.; Cancer. Res. 44, 5544 (1984) Abstract
[4] Use of fluorescent dyes as molecular probes for the study of multidrug resistance: A.A. Neyfakh; Exp. Cell. Res. 174, 168 (1988) Abstract
[5] Reversal of resistance to rhodamine 123 in adriamycin-resistant Friend leukemia cells: T.J. Lampidis, et al.; Cancer. Res. 45, 2626 (1985) Abstract
[6] Detection of activity of P-glycoprotein in human tumour samples using rhodamine 123: C. Ludescher, et al.; Br. J. Haematol. 82, 161 (1992) Abstract
[7] Characterization of rhodamine 123 binding to P-glycoprotein in human multidrug-resistant cells: B. Nare, et al.; Mol. Pharmacol. 45, 1145 (1994) Abstract
[8] A comparison of rhodamine 123 accumulation and efflux in cells with P- glycoprotein-mediated and MRP-associated multidrug resistance phenotypes: P.R. Twentyman, et al.; Eur. J. Cancer. 30A, 1360 (1994) Abstract
[9] Stoichiometry of coupling of rhodamine 123 transport to ATP hydrolysis by P-glycoprotein: A.B. Shapiro & V. Ling; Eur. J. Biochem. 254, 189 (1998) Abstract
[10] Unidirectional fluxes of rhodamine 123 in multidrug-resistant cells: evidence against direct drug extrusion from the plasma membrane: G.A. Altenberg, et al.; PNAS 91, 4654 (1994) Abstract
[11] Carrier-mediated uptake of rhodamine 123: implications on its use for MDR research: C.W. Cho, et al.; BBRC 279, 124 (2000) Abstract
[12] Altered multidrug resistance phenotype caused by anthracycline analogues and cytosine arabinoside in myeloid leukemia: X.F. Hu, et al.; Blood 93, 4086 (1999) Abstract

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