ALEXIS Biochemicals: SN50

ALX-167-024

Revised 17-May-06

SN50
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	NF-kB Pathway Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-167-024-C500		500 µg	125.00 USD

Product Specification

SEQUENCE:	H-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Val-Gln-Arg-Lys-Arg-Gln-Lys-Leu-Met-Pro-OH
FORMULA:	C₁₂₉H₂₃₀N₃₆O₂₉S
MW:	2781.5
PEPTIDE CONTENT:	50-75%
PURITY:	≥96% (HPLC)
APPEARANCE:	White to off-white solid.
FORMULATION:	Counter Ion: Trifluoroacetate salt.
SOLUBILITY:	Soluble in distilled water, 100% ethanol or ethanol:water 1:1.
RECONSTITUTION:	Reconstitute in sterile distilled water.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Peptides in solution have limited stability.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Description

Inhibits translocation of the NF-κB active complex into the nucleus. In mouse endothelial LE-II cells induced with LPS, inhibition of NF-κB nuclear translocation was 88% at 50µg/ml (18µM). In human monocytic THP-1 cells induced by LPS or TNF-α there was 85% inhibition at 100µg/ml. For control peptide, see SN50M (Prod. No. ALX-167-025).

Product Specific Literature References

Inhibition of nuclear translocation of transcription factor NF-kappa B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence: Y.Z. Lin, et al.; J. Biol. Chem. 270, 14255 (1995) Abstract; Full Text
p38 MAP kinase and MKK-1 co-operate in the generation of GM-CSF from LPS-stimulated human monocytes by an NF-kappa B-independent mechanism: K.K. Meja, et al.; Br. J. Pharmacol. 131, 1143 (2000) Abstract
Nuclear factor-kappaB activates dual inhibition sites in the regulation of tumor necrosis factor-alpha-induced neutrophil apoptosis: M. Niwa, et al.; Eur. J. Pharmacol 407, 211 (2000) Abstract
Activation of nuclear factor kappaB and induction of apoptosis by tumor necrosis factor-alpha in the mouse uterine epithelial WEG-1 cell line: S. Pampfer, et al.; Biol. Reprod. 63, 879 (2000) Abstract
Interruption of the NF-kappaB pathway by Bay 11-7082 promotes UCN-01-mediated mitochondrial dysfunction and apoptosis in human multiple myeloma cells: Y. Dai, et al.; Blood 103, 2761 (2004) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Nuclear localization sequence (NLS) aa 360-369 (AAVALLPAVLLALLAPVQRKRQKLMP) of the transcription factor NF-κB p50 linked to a peptide cell-permeablization sequence, the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF).

Further Categories Containing This Product:

Nuclear Envelope & Nuclear Transport • Peptides

another CS2 website

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.