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MAP Kinase Pathways
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ALX-155-024 Revised 04-Feb-05
ERT Protein Kinase Substrate
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY MAPK Pathway Substrates
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-155-024-M001   1 mg 90.00 USD Add To Cart
ALX-155-024-M005   5 mg 360.00 USD Add To Cart
Product Specification
SEQUENCE: H-Lys-Arg-Glu-Leu-Val-Glu-Pro-Leu-Thr-Pro-Ser-Gly-Glu-Ala-Pro-Asn-Gln-Ala-Leu-Leu-Arg-OH
FORMULA: C101H172N30O32
MW: 2318.7
PURITY: ≥97%
APPEARANCE: White lyophilized powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Substrate for the growth factor-stimulated (MAP2-related) protein kinase (ERT protein kinase). The peptide covers the ERT protein kinase phosphorylation site aa 689-705 of human precursor epidermal growth factor (EGF) receptor at Thr669 (mouse Thr671) .
Product Specific Literature References
Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c- myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase: E. Alvarez, et al.; J. Biol. Chem. 266, 15277 (1991) Abstract; Full Text
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Peptides
 
 
ALX-155-026 Revised 04-Jul-03
ERT Protein Kinase Substrate Amide
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY MAPK Pathway Substrates
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ALX-155-026-M001   1 mg 80.00 USD Add To Cart
ALX-155-026-M005   5 mg 280.00 USD Add To Cart
Product Specification
SEQUENCE: H-Lys-Arg-Glu-Leu-Val-Glu-Pro-Leu-Thr-Pro-Ser-Gly-Glu-Ala-Pro-Asn-Gln-Ala-Leu-Leu-Arg-NH2
FORMULA: C101H173N31O31
MW: 2317.7
PURITY: ≥97%
APPEARANCE: White lyophilized powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Substrate for the growth factor-stimulated (MAP2-related) protein kinase (ERT protein kinase). The peptide covers the ERT protein kinase phosphorylation site aa 689-705 of human precursor epidermal growth factor receptor (EGFR) at Thr669 (mouse Thr671).
Product Specific Literature References
Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c- myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase: E. Alvarez, et al.; J. Biol. Chem. 266, 15277 (1991) Abstract; Full Text
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Peptides
 
 
ALX-159-600 Revised 14-Jul-08
JNK Inhibitor 1 (L-stereoisomer)
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SYNONYMS L-JNKI1
c-Jun N-terminal Kinase Peptide Inhibitor 1, L-stereoisomer
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY JNK [SAPK1]/Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-159-600-R100   100 µl 125.00 USD Add To Cart
ALX-159-600-R200   200 µl 225.00 USD Add To Cart
Product Specification
SEQUENCE: GRKKRRQRRR-PP-RPKRPTTLNLFPQVPRSQD-amide
FORMULA: C164H286N66O40
MW: 3822.5
CONCENTRATION: 1mM
PURITY: ≥97% (HPLC)
FORMULATION: Liquid. Sterile, ready-to-use solution in PBS.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Keep on ice at all times.
POSITIVE CONTROL: Prod. No. ALX-168-009/1
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Product Description

Cell permeable JNK (c-Jun N-terminal kinase) inhibitor. For increased cell permeability the peptide was covalently linked to the 10 aa recognized by the TAT transporter. Neuroprotective agent for stroke.
Product Specific Literature References
[1] Cell-permeable peptide inhibitors of JNK: novel blockers of beta-cell death: C. Bonny, et al.; Diabetes 50, 77 (2001) Abstract; Full Text
Hyperosmolarity and CD95L trigger CD95/EGF receptor association and tyrosine phosphorylation of CD95 as prerequisites for CD95 membrane trafficking and DISC formation: R. Reinehr, et al.; FASEB J. 17, 731 (2003) Abstract
Translation inhibitors sensitize prostate cancer cells to TRAIL-induced apoptosis by activating c-Jun N-terminal kinase: N.K. Sah, et al.; J. Biol. Chem. 278, 20593 (2003) Abstract; Full Text
A peptide inhibitor of c-Jun N-terminal kinase protects against excitotoxicity and cerebral ischemia: T. Borsello, et al.; Nat. Med. 9, 1180 (2003) Abstract
Activation of JNK in sensory neurons protects against sensory neuron cell death in diabetes and on exposure to glucose/oxidative stress in vitro: S.A. Price, et al.; Ann. NY Acad. Sci. 1010, 95 (2003)
Insulin-secreting beta-cell dysfunction induced by human lipoproteins: M.E. Roehrich, et al.; J. Biol. Chem. 278, 18368 (2003) Abstract; Full Text
Inhibition of c-Jun NH2-terminal kinase activity improves ischemia/reperfusion injury in rat lungs: M. Ishii, et al.; J. Immunol. 172, 2569 (2004) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION A protein inhibitor named IB1 has been described that competitively blocks the interaction between JNK and c-Jun, thereby inhibiting the signalling events downstream of JNK [2,3] like c-Jun, ATF2 and ELK1 phosphorylation. To convert IB1 into cell-permeable inhibitors of JNK (JNKI peptides) the minimal 20 aa inhibitory sequence of IB1 was covalently linked to the 10 amino acids recognized by TAT transporter [4].
The L-JNKI1 and the protease resistant D-JNKI1 peptides represent the only potent inhibitors that are specific for JNK (JNK1, JNK2 and JNK3). Different from chemical inhibitors that directly affect kinase activity e.g. by competing with the ATP-binding site of the protein kinase, JNKI1 rather inhibits the interaction between JNK and its substrate, resulting in a JNK K.O. phenotype. In contrast to pure diffusion the TAT-peptides are actively transported into cells, where they remain until their proteolytic degradation. They can be used for in vitro as well as for in vivo applications (IC50~1µM).
D-JNKI1 is the only form found to be active on neuronal cells, probably due to a high level of proteolytic degradation of the L-stereoisomer (T. Borsello and C. Bonny; unpublished data). As these peptides tend to be toxic in neurons above concentrations of 5µM, it is recommended to use concentrations of ~1µM or less. When interpreting results of c-Jun inhibition, one should also note that a number of other c-Jun kinases are thought to contribute to c-Jun phosphorylation (C. Bonny, personal communication).

*For in vivo applications in mice it is suggested to start with 30-50µl of a 1mM stock solution administered intraperitoneally. Cellular uptake of L-JNKI1 peptides can now be confirmed using the FITC-conjugated L-TAT control peptide (Prod. No. ALX-168-009F).
Note: L-TAT control peptides should only be used with L-JNKI1.
General Literature References
[2] IB1, a JIP-1-related nuclear protein present in insulin-secreting cells: C. Bonny, et al.; J. Biol. Chem. 273, 1843 (1998) Abstract; Full Text
[3] A cytoplasmic inhibitor of the JNK signal transduction pathway: M. Dickens, et al.; Science 277, 693 (1997) Abstract
[4] In vivo protein transduction: delivery of a biologically active protein into the mouse: S.R. Schwarze, et al.; Science 285, 1569 (1999) Abstract
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Further Categories Containing This Product:
MAPK Pathway Inhibitors • Peptides
 
 
ALX-168-005 Revised 04-Mar-05
Tyrosine Hydroxylase (24-33) (rat)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY MAPK Pathway Substrates
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ALX-168-005-M001   1 mg 85.00 USD Add To Cart
Product Specification
SEQUENCE: H-Lys-Gln-Ala-Glu-Ala-Val-Thr-Ser-Pro-Arg-OH
FORMULA: C45H79N15O16
MW: 1086.2
PURITY: ≥98%
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Represents consensus site for MAP kinase (MAPK/ERK) phosphorylation. Includes the Ser31 phosphorylation site.
Product Specific Literature References
ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ: J.W. Haycock, et al.; PNAS 89, 2365 (1992) Abstract
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Peptides
 
 
ALX-168-009/1 Revised 22-Jul-08 New product
L-TAT Control Peptide (for Prod. No. ALX-159-600)
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SYNONYMS JNK Inhibitor 1 (L-stereoisomer) Control Peptide (for Prod. No. ALX-159-600)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY JNK [SAPK1]/Related Products
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ALX-168-009/1-R050   50 µl 40.00 USD Add To Cart
Product Specification
SEQUENCE: GRKKRRQRRR-amide
FORMULA: C55H109N31O12
MW: 1396.6
CONCENTRATION: 10mM
PURITY: ≥85% (HPLC)
FORMULATION: Liquid. Sterile solution in water.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles. Keep on ice at all times.
Product Description
Control peptide for L-JNKI. For more details see L-JNKI1 (Prod.No. ALX-159-600).
Product Specific Literature References
Cell-permeable peptide inhibitors of JNK: novel blockers of beta-cell death: C. Bonny, et al.; Diabetes 50, 77 (2001) Abstract; Full Text
Hyperosmolarity and CD95L trigger CD95/EGF receptor association and tyrosine phosphorylation of CD95 as prerequisites for CD95 membrane trafficking and DISC formation: R. Reinehr, et al.; FASEB J. 17, 731 (2003) Abstract; Full Text
Related Products
Further Categories Containing This Product:
Peptides
 
 
ALX-168-009F Revised 28-Jul-08 New product
L-TAT Control Peptide (for Prod. No. ALX-159-600) (FITC)
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SYNONYMS JNK Inhibitor 1 (L-stereoisomer) Control Peptide (for Prod. No. ALX-159-600) (FITC)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY JNK [SAPK1]/Related Products
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ALX-168-009F-R050 FITC 50 µl 110.00 USD Add To Cart
Product Specification
SEQUENCE: FITC-β-A-RKKRRQRRRA-amide
FORMULA: C80H128N34O17S
MW: 1870.2
CONCENTRATION: 1mM
PURITY: ≥90% (HPLC)
FORMULATION: Liquid. In sterile PBS.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. Keep on ice at all times.
Product Description
Control peptide for L-JNKI. For more details see L-JNKI1 (Prod.No. ALX-159-600).
Product Specific Literature References
Cell-permeable peptide inhibitors of JNK: novel blockers of beta-cell death: C. Bonny, et al.; Diabetes 50, 77 (2001) Abstract; Full Text
Hyperosmolarity and CD95L trigger CD95/EGF receptor association and tyrosine phosphorylation of CD95 as prerequisites for CD95 membrane trafficking and DISC formation: R. Reinehr, et al.; FASEB J. 17, 731 (2003) Abstract; Full Text
Related Products
Further Categories Containing This Product:
Peptides
 
 
ALX-168-010 Revised 14-Aug-06
D-TAT Control Peptide (for Prod. No. ALX-159-601)
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SYNONYMS JNK Inhibitor 1 (D-stereoisomer) Control Peptide (for Prod. No. ALX-159-601)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY JNK [SAPK1]/Related Products
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ALX-168-010-R025   25 µl 95.00 USD Add To Cart
ALX-168-010F-R025 FITC 25 µl 145.00 USD Add To Cart
Product Specification
SEQUENCE: Unconjugated peptide: rrrqrrkkr-amide
FITC-conjugated peptide: FITC-β-a-rkkrrqrrra-amide
FORMULA: Unconjugated peptide: C53H107N31O10
FITC-conjugated peptide: C80H128N34O17S
MW: Unconjugated peptide: 1338.6
FITC-conjugated peptide: 1870.2
CONCENTRATION: 1mM
PURITY: ≥95% (HPLC)
FORMULATION: Liquid. In sterile PBS.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Protect from moisture. Avoid freeze/thaw cycles. Keep on ice at all times.
Product Images
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Product Description
Control peptide for D-JNKI. For more details see JNK Inhibitor 1 (D-stereoisomer) (Prod. No. ALX-159-601).
Product Specific Literature References
Cell-permeable peptide inhibitors of JNK: novel blockers of beta-cell death: C. Bonny, et al.; Diabetes 50, 77 (2001) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Note: D-TAT consists of D-amino acids. The sequence of unconjugated D-TAT is retro-inverse compared to L-TAT.
Further Categories Containing This Product:
Peptides
 
 
ALX-200-606 Revised 08-May-08
Myelin Basic Protein (human) (high purity)
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SYNONYMS MBP (human) (high purity)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Myelin Basic Protein/Related Products
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ALX-200-606-M001   1 mg 350.00 USD Add To Cart
Product Specification
SOURCE/HOST: Purified from human brain by method of Määttä et al. [1].
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE; Immunoblot)
APPEARANCE: White to off-white powder.
FORMULATION: Lyophilized. Essentially salt-free.
RECONSTITUTION: Reconstitute with distilled water.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year when stored at +4°C. Reconstituted protein is stable for at least six months at -20°C and for at least 24 hours at +37°C in aqueous solution.
Product Specific Literature References
[1] Detection of myelin basic protein isoforms by organic concentration: J.A. Määttä, et al.; BBRC 238, 498 (1997) Abstract
Interaction between the C-terminal region of human myelin basic protein and calmodulin: analysis of complex formation and solution structure: V. Majava, et al.; BMC Struct. Biol. 8, 10 (2008) Abstract; Full Text
General Information
MBP is a peripheral membrane protein, and its interaction with lipids is generally believed to be critical for the formation and stability of the multilamellar myelin sheath. It has been identified as a Ca2+-calmodulin regulated agent for actin polymerization, tropomyosin and actomyosin function, tubulin stabilization, and clathrin assembley. MBP is generally considered to be the antigen responsible for autoimmunity in multiple sclerosis (MS) and it has the capability to induce experimental allergic encephalomyelitis (EAE). MBP is also an endogenous inhibitor of the high-affinity cannabinoid binding site in the brain and a substrate for numerous protein kinases (MAPK, serine/threonine kinase, protein kinase C). MBP is also suggested to be involved in intracellular signalling and as cytoskeletal components.
Further Categories Containing This Product:
MAPK Pathway Substrates • CAM Kinase Substrates • Natural Proteins
 
 
ALX-201-408 Revised 27-Mar-08
Ribosomal Protein S3 (human) (recombinant) (His)
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SYNONYMS RPS3 (human) (recombinant) (His)
40S Ribosomal Protein S3
IMR-90 Ribosomal Protein S3
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Ribosomal Proteins/Related Products
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ALX-201-408-C010   10 µg 330.00 USD Add To Cart
ALX-201-408-C050   50 µg 740.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. Mature peptide of human ribosomal protein S3 (aa 1-243) is fused at the C-terminus to a His-tag.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in 25mM TRIS-HCl, pH 8.5, containing 1mM DTT.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Interaction of Hsp90 with ribosomal proteins protects from ubiquitination and proteasome-dependent degradation: T.S. Kim, et al.; Mol. Biol. Cell 17, 824 (2006) Abstract; Full Text
Reduction of invasion in human fibrosarcoma cells by ribosomal protein S3 in conjunction with Nm23-H1 and ERK: S.H. Kim & J. Kim; Biochim. Biophys. Acta 1763, 823 (2006) Abstract
Ribosomal protein S3: a KH domain subunit in NF-kappaB complexes that mediates selective gene regulation: F. Wan, et al.; Cell 131, 927 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P23396: Ribosomal Protein S3 (human)
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
Co-Chaperones & HSP-interacting Proteins/Related Products • Recombinant Proteins/Fusion Proteins • DNA Endonucleases/Related Products • NF-kB Pathway Other Products • MAPK Pathway Other Products
 
 
ALX-201-410 Revised 13-Feb-08
Ribosomal Protein S3 (human) (recombinant)