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ALX-804-388
Revised 20-Sep-07
Monoclonal Antibody to Casein Kinase II Substrate (phosphorylated) (9F4)
SYNONYMS
anti-CKII Substrate (phosphorylated) MAb (9F4)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Casein Kinase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-388-C100
100 µg
Product Specification
SPECIES CROSSREACTIVITY:
Others
CLONE:
9F4
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
Synthetic phosphopeptide corresponding to CKII (casein kinase II) substrate consensus phosphorylation site of activated CKII substrate.
SPECIFICITY:
Recognizes DpSD motif of activated CKII substrate in a broad range of species.
APPLICATION:
ELISA (non-radioactive quantification of phospho-peptides in kinase/phosphatase assays; use at 0.1-1µg/ml).
Note:
Not suitable for Western blot!
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Phospho-specific Antibodies
•
Monoclonal Antibodies
ALX-804-260/1
Revised 09-Oct-08
Monoclonal Antibody to β-Catenin (8E4)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Catenin / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-260/1-C100
100 µg
380.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE:
8E4
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute in 1ml distilled water (15 min., RT).
IMMUNOGEN:
Synthetic peptide corresponding to human β-catenin aa 27-37.
SPECIFICITY:
Recognizes human, mouse, rat (weak) and dog β-catenin. Recognizes an epitope between aa 27-37 and an additional epitope in the C-Terminal region (see [5]).
Note:
For specific detection of dephospho-β-catenin use Prod. No.
ALX-804-259
.
APPLICATION:
Flow Cytometry
(see Lit. Ref. [3])
Immunocytochemistry
(see Lit. Ref. [4])
Immunohistochemistry
(paraffin sections) (see Lit. Ref. [3])
Western Blot
: 0.5µg/ml for HRPO/ECL detection. Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
BSA may be used alternatively but milk is not recommended for blocking and dilution!
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL:
Included (Prod. No. ALX-840-022).
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[2]
Sulindac targets nuclear beta-catenin accumulation and Wnt signalling in adenomas of patients with familial adenomatous polyposis and in human colorectal cancer cell lines
:
E.M. Boon, et al.; Br. J. Cancer
90
, 224 (2004)
Abstract
[1]
Activation of the canonical beta-catenin pathway by histamine
:
S.H. Diks, et al.; J. Biol. Chem.
278
, 52491 (2003)
Abstract
;
Full Text
[3]
Illegitimate WNT signaling promotes proliferation of multiple myeloma cells
:
P.W. Derksen, et al.; PNAS
101
, 6122 (2004)
Abstract
;
Full Text
[4]
Modulation of intracellular beta-catenin localization and intestinal tumorigenesis in vivo and in vitro by sphingolipids
:
E.M. Schmelz, et al.; Cancer Res.
61
, 6723 (2001)
Abstract
[5]
Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools:
M. van Noort, et al.; Blood
110
, 2778 (2007)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-101
Revised 04-Jun-08
Monoclonal Antibody to α-Catenin (15D9)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Catenin / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-101-C100
100 µg
380.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE:
15D9
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
Recombinant human α-catenin.
SPECIFICITY:
Recognizes human, mouse, rat and dog α-catenin. Does not cross-react with β-catenins.
APPLICATION:
Immunocytochemistry
(0.1-1µg/ml; formaldehyde fixation is tolerated)
Immunoprecipitation
Western Blot
(0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:
Yes (Prod. No. ALX-840-026).
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-106
Revised 13-Jun-08
Monoclonal Antibody to β-Catenin (CT) (10H8)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Catenin / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-106-C100
100 µg
380.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE:
10H8
ISOTYPE:
Mouse IgG2a
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and Sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
Recombinant human β-catenin.
SPECIFICITY:
Recognizes the C-terminus of human, mouse, rat (weak) and dog β-catenin (aa 769-781).
APPLICATION:
Immunocytochemistry
(0.1-1µg/ml)
Immunoprecipitation
Western blot
(0.1µg/ml for HRPO/ECL detection).
Recommended blocking buffer milk: 2x PBS, 5-8% (w/v) dry milk powder and 0.1% (v/v) Tween 20.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:
Included (Prod. No. ALX-840-027).
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-059
Revised 13-Jun-08
Monoclonal Antibody to β-Catenin (CT/Exon 14) (7D8)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Catenin / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-059-C100
100 µg
380.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE:
7D8
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
Recombinant human β-catenin.
SPECIFICITY:
Recognizes the C-terminus (exon 14) of human, mouse, rat (weak) and a dog β-catenin.
APPLICATION:
Immunocytochemistry
(0.1-1µg/ml)
Immunohistochemistry
(paraffin sections)
I
mmunoprecipitation
Western blot
(0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:
Included (Prod. No. ALX-840-027).
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-259
Revised 09-Oct-08
Monoclonal Antibody to β-Catenin (dephospho, aa 35-50) (7A7)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Catenin / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-259-C100
100 µg
380.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE:
7A7
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
Synthetic peptide corresponding to aa 35-50 of human β-catenin.
SPECIFICITY:
Recognizes human, mouse, rat (weak) and dog cytoplasmic dephospho β-catenin.
APPLICATION:
Immunocytochemistry
(1-10µg/ml)
Immunohistochemistry
(paraffin sections)
Immunoprecipitation
Western Blot
(1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
BSA may be used alternatively but milk is not recommended for blocking and dilution!
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL:
Included (Prod. No. ALX-840-022).
Product Images
Please click on thumbnails to enlarge.
Related Products
ALX-804-060
Monoclonal Antibody to β-Catenin (NT/Exon 2) (7D11)
ALX-804-058
Monoclonal Antibody to β-Catenin (Exon 3) (9G2)
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-058
Revised 13-Jun-08
Monoclonal Antibody to β-Catenin (Exon 3) (9G2)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Catenin / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-058-C100
100 µg
380.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE:
9G2
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN:
Recombinant human β-catenin.
SPECIFICITY:
Recognizes exon 3 (α-catenin-binding site) of human, mouse, rat (weak) and dog β-catenin.
APPLICATION:
Immunocytochemistry
(0.1-1µg/ml)
Immunoprecipitation
Western Blot
(0.1µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED:
Yes (Prod. No. ALX-840-027)
Product Images
Please click on thumbnails to enlarge.
Related Products
ALX-804-060
Monoclonal Antibody to β-Catenin (NT/Exon 2) (7D11)
ALX-804-259
Monoclonal Antibody to β-Catenin (dephospho, aa 35-50) (7A7)
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-060
Revised 13-Jun-08
Monoclonal Antibody to β-Catenin (NT/Exon 2) (7D11)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Catenin / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-060-C100
100 µg
380.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE:
7D11
ISOTYPE:
Mouse IgG2a
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
Recombinant human β-catenin.
SPECIFICITY:
Recognizes the N-terminus (exon 2) of human, mouse, rat (weak) and dog β-catenin.
APPLICATION:
Immunoprecipitation
Western Blot
(0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:
Included (Prod. No. ALX-840-027).
Product Images
Please click on thumbnails to enlarge.
Related Products
ALX-804-259
Monoclonal Antibody to β-Catenin (dephospho, aa 35-50) (7A7)
ALX-804-058
Monoclonal Antibody to β-Catenin (Exon 3) (9G2)
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-548
Revised 14-Jul-07
Monoclonal Antibody to GSK-3β (11B9)
SYNONYMS
anti-Glycogen Synthase Kinase-3β MAb (11B9)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
GSK-3 Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-548-C100
100 µg
453.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE:
11B9
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN:
Synthetic peptide corresponding to the activation loop of GSK-3β
(
glycogen synthase kinase-3β
).
SPECIFICITY:
Recognizes the activation loop of human, mouse, rat and dog GSK-3β.
APPLICATION:
Western Blot
(0.1µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED:
Yes (Prod. No. ALX-840-030)
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-374
Revised 14-Feb-06
Monoclonal Antibody to GSK-3β (phosphorylated) (pSer9) (2D3)
SYNONYMS
anti-Glycogen Synthase Kinase-3β (phosphorylated) (pSer
9
) MAb (2D3)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
GSK-3 Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-374-C100
100 µg
453.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE:
2D3
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2xPBS, containing 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN:
Synthetic peptide corresponding to a portion of phosphorylated GSK-3β (glycogen synthase kinase-3β).
SPECIFICITY:
Recognizes human, mouse and dog GSK-3β phosphorylated at Ser
9
. Does not cross-react with non-phosphorylated GSK-3β, GSK-3α or phosphorylated GSK-3α.
APPLICATION:
Western Blot
(1µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED:
yes
Further Categories Containing This Product:
Phospho-specific Antibodies
•
Monoclonal Antibodies
ALX-804-178
Revised 11-Apr-07
Monoclonal Antibody to GSK-3β (1H8)
SYNONYMS
anti-Glycogen Synthase Kinase-3β MAb
(1H8)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
GSK-3 Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-178-C100
100 µg
