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Signal Transduction
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ALX-340-029 Revised 08-Jul-08
Anandamide
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SYNONYMS AEA
N-Arachidonoylethanolamine
(all-Z)-N-(2-Hydroxyethyl)-5,8,11,14-eicosatetraenamide
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Anandamide & Anandamide Analogs
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-340-029-M005   5 mg 51.00 USD Add To Cart
Product Specification
FORMULA: C22H37NO2
MW: 347.5
CAS NUMBER: 94421-68-8
MERCK INDEX: 14: 624
RTECS: JX3842500
CONCENTRATION: 50mg/ml 
PURITY: ≥98%
FORMULATION: Liquid. Solution in ethanol.
SOLUBILITY: 10mg/ml soluble in DMSO or dimethyl formamide.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 6 months in other organic solvents when stored at -20°C.
HANDLING: Protect from light and oxygen.

Product Description
Endogenous [1] ligand for the CB1 receptor (CB1: Ki=52nm; CB2: Ki=1930nm [2]) and TRPV1 (Ki=5.78µM [3,4]). Inhibits NF-κB activation through direct binding to IKKβ [5] and induces apoptosis independently of cannabinoid or vanilloid receptors [6]. Activates the MAP kinase (MAPK/ERK) signalling pathway [7].
Product Specific Literature References
[1] Isolation and structure of a brain constituent that binds to the cannabinoid receptor: W.A. Devane, et al.; Science 258, 1946 (1992) Abstract
[2] Novel analogues of arachidonylethanolamide (anandamide): affinities for the CB1 and CB2 cannabinoid receptors and metabolic stability: S. Lin, et al.; J. Med. Chem. 41, 5353 (1998) Abstract
[3] Vanilloid receptors on sensory nerves mediate the vasodilator action of anandamide: P.M. Zygmunt, et al.; Nature 400, 452 (1999) Abstract
[4] Structure-activity relationship for the endogenous cannabinoid, anandamide, and certain of its analogues at vanilloid receptors in transfected cells and vas deferens: R.A. Ross, et al.; Br. J. Pharmacol. 132, 631 (2001) Abstract
[5] Anandamide inhibits nuclear factor-kappaB activation through a cannabinoid receptor-independent pathway: R. Sancho, et al.; Mol. Pharmacol. 63, 429 (2003) Abstract
[6] Anandamide induces cell death independently of cannabinoid receptors or vanilloid receptor 1: possible involvement of lipid rafts: K.P. Sarker, et al.; Cell Mol. Life Sci. 60, 1200 (2003) Abstract
[7] The MAP kinase signal transduction pathway is activated by the endogenous cannabinoid anandamide: M. Wartmann, et al.; FEBS Lett. 359, 133 (1995) Abstract
Biochemistry and pharmacology of arachidonylethanolamide, a putative endogenous cannabinoid: C.J. Hillard & W.B. Campbell; J. Lipid Res. 38, 2383 (1997), (Review) Abstract
The endogenous cannabinoid anandamide inhibits human breast cancer cell proliferation: L. De Petrocellis, et al.; PNAS 95, 8375 (1998) Abstract
Cannabinoid receptors and their endogenous agonist, anandamide: J. Axelrod & C.C. Felder; Neurochem. Res. 23, 575 (1998), (Review) Abstract
Endocannabinoids: endogenous cannabinoid receptor ligands with neuromodulatory action: V. Di Marzo, et al.; TINS 21, 521 (1998), (Review) Abstract
Endocannabinoids: a new class of vasoactive substances: M.D. Randall & D.A. Kendall; TIPS 19, 55 (1998), (Review) Abstract
Metabolism of anandamide and 2-arachidonoylglycerol: an historical overview and some recent developments: V. Di Marzo, et al.; Lipids 34, S319 (1999), (Review) Abstract
Anandamide: a candidate neurotransmitter heads for the big leagues: D.W. Self; Nat. Neurosci. 2, 303 (1999), (Review) Abstract
New perspectives on enigmatic vanilloid receptors: A. Szallasi & V. Di Marzo; TINS 23, 491 (2000), (Review) Abstract
Anandamide: some like it hot: V. Di Marzo, et al.; TIPS 22, 346 (2001), (Review) Abstract
Anandamide is an endogenous inhibitor for the migration of tumor cells and T lymphocytes: J. Joseph, et al.; Cancer Immunol. Immunother. 53, 723 (2004) Abstract
Anandamide-induced cell death in primary neuronal cultures: role of calpain and caspase pathways: V.A. Movsesyan, et al.; Cell Death Differ. 11, 1121 (2004) Abstract
Arachidonylethanolamide induces apoptosis of human glioma cells through vanilloid receptor-1: E. Contassot, et al.; J. Neuropathol. Exp. Neurol. 63, 956 (2004) Abstract
Preferred conformations of endogenous cannabinoid ligand anandamide: J.Z. Chen, et al.; Life Sci. 76, 2053 (2005) Abstract
The cardiovascular actions of anandamide: more targets?: M.D. Randall; Br. J. Pharmacol. 145, 565 (2005) Abstract; Full Text
Accumulation of anandamide: Evidence for cellular diversity: C.J. Hillard and A. Jarrahian; Neuropharmacology 48, 1072 (2005) Abstract
Anandamide as an intracellular messenger regulating ion channel activity: M. van der Stelt and V. Di Marzo; Prostaglandins Other Lipid Mediat. 77, 111 (2005) Abstract
Anandamide reduces infarct size in rat isolated hearts subjected to ischaemia-reperfusion by a novel cannabinoid mechanism: N.J. Underdown, et al.; Br. J. Pharmacol. 146, 809 (2005) Abstract
Anandamide inhibits adhesion and migration of breast cancer cells: C. Grimaldi, et al.; Exp. Cell Res. 312, 363 (2006) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION To change the solvent, evaporate the ethanol under a gentle stream of nitrogen and immediately add the solvent of choice.
 
 
ALX-151-037 Revised 23-Nov-06
Angiotensin I
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin & Angiotensin Receptors/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-151-037-M005   5 mg 30.00 USD Add To Cart
ALX-151-037-M025   25 mg 125.00 USD Add To Cart
Product Specification
SEQUENCE: H-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-OH
FORMULA: C62H89N17O14
MW: 1296.5
APPEARANCE: Lyophilized.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Peptides
 
 
ALX-151-038 Revised 23-Nov-06
Angiotensin I (1-9)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin & Angiotensin Receptors/Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-151-038-M005   5 mg 75.00 USD Add To Cart
Product Specification
SEQUENCE: H-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-OH
FORMULA: C56H78N16O13
MW: 1183.3
APPEARANCE: Lyophilized.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Peptides
 
 
ALX-151-039 Revised 22-Jan-07
Angiotensin II
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin & Angiotensin Receptors/Related Products
Ordering Information
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ALX-151-039-M005   5 mg 30.00 USD Add To Cart
ALX-151-039-M025   25 mg 125.00 USD Add To Cart
Product Specification
SEQUENCE: H-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-OH
FORMULA: C50H71N13O12
MW: 1046.2
PURITY: ≥98% (HPLC)
APPEARANCE: Lyophilized.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Peptides
 
 
ALX-151-040 Revised 23-Nov-06
Angiotensin I/II (1-5)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin & Angiotensin Receptors/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-151-040-M005   5 mg 95.00 USD Add To Cart
Product Specification
SEQUENCE: H-Asp-Arg-Val-Tyr-Ile-OH
FORMULA: C30H48N8O9
MW: 664.7
APPEARANCE: Lyophilized.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Peptides
 
 
ALX-151-041 Revised 19-Jan-07
Angiotensin I/II (1-7)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin & Angiotensin Receptors/Related Products
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ALX-151-041-M005   5 mg 90.00 USD Add To Cart
Product Specification
SEQUENCE: H-Asp-Arg-Val-Tyr-Ile-His-Pro-OH
FORMULA: C41H62N12O11
MW: 899.0
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white powder.
FORMULATION: TFA salt.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Peptides
 
 
ALX-151-043 Revised 07-Feb-08
Angiotensin III
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin & Angiotensin Receptors/Related Products
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ALX-151-043-M005   5 mg 35.00 USD Add To Cart
ALX-151-043-M025   25 mg 140.00 USD Add To Cart
Product Specification
SEQUENCE: H-Arg-Val-Tyr-Ile-His-Pro-Phe-OH
FORMULA: C46H66N12O9
MW: 931.1
APPEARANCE: Lyophilized.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Peptides
 
 
ALX-151-042 Revised 11-Dec-07
Angiotensin IV
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SYNONYMS Angiotensin I/II (3-8)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin & Angiotensin Receptors/Related Products
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ALX-151-042-M005   5 mg 45.00 USD Add To Cart
Product Specification
SEQUENCE: H-Val-Tyr-Ile-His-Pro-Phe-OH
FORMULA: C40H54N8O8
MW: 774.9
PURITY: ≥99%
APPEARANCE: Lyophilized.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Peptides
 
 
ALX-380-051 Revised 20-Jun-08
Anisomycin
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SYNONYMS 2-(p-Methoxybenzyl)-3,4-pyrrolidinediol-3-acetate
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics - Other Signal Transduction Pathway Modulators
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-380-051-M010   10 mg 35.00 USD Add To Cart
ALX-380-051-M050   50 mg 140.00 USD Add To Cart
ALX-380-051-M100   100 mg 250.00 USD Add To Cart
Product Specification
FORMULA: C14H19NO4
MW: 265.3
CAS NUMBER: 22862-76-6
MERCK INDEX: 14: 670
RTECS: BZ9800000
SOURCE/HOST: Isolated from Streptomyces griseolus.
PURITY: ≥98% (HPLC)
APPEARANCE: White crystalline solid.
SOLUBILITY: Soluble in DMSO (25mg/ml), 100% ethanol, methanol or ethyl acetate (10mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: TOXIC.

Product Description
Antibiotic. Activator of p38 and MAP kinases. Synergistic with growth factors and phorbol esters to superinduce cFos and cJun, by acting as a potent signalling agonist. Induces apoptosis in the human monoblastoid cell line. Used in the eradication of bean mildew. Inhibits other pathogenic fungi in plants.
Product Specific Literature References
Anisomycin, a new anti-protozoan antibiotic: B. A. Sobin & F. W. Tanner; J. Am. Chem. Soc. 76, 4053 (1954)
DNA fragmentation and cytolysis in U937 cells treated with diphtheria toxin or other inhibitors of protein synthesis: S.K. Kochi & R.J. Collier; Exp. Cell. Res. 208, 296 (1993) Abstract
The stress-activated protein kinase subfamily of c-Jun kinases: J.M. Kyriakis, et al.; Nature 369, 156 (1994) Abstract
Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun: I. Sanchez, et al.; Nature 372, 794 (1994) Abstract
Anisomycin and rapamycin define an area upstream of p70/85S6k containing a bifurcation to histone H3-HMG-like protein phosphorylation and c-fos-c-jun induction: E. Kardalinou, et al.; Mol. Cell. Biol. 14, 1066 (1994) Abstract
Protein synthesis inhibitor phase shifts vasopressin rhythms in long- term suprachiasmatic cultures: K. Shinohara & T. Oka; NeuroReport 5, 2201 (1994) Abstract
Anisomycin selectively desensitizes signalling components involved in stress kinase activation and fos and jun induction: C.A. Hazzalin, et al.; Mol Cell Biol 18, 1844 (1998) Abstract
Anisomycin treatment paradigm affects duration of long-term potentiation in slices of the amygdala: P. Okulski, et al.; Neuroscience 114, 1 (2002) Abstract
Downregulation of Ski and SnoN co-repressors by anisomycin: A. Vazquez-Macias, et al.; FEBS Lett. 579, 3701 (2005) Abstract
Anisomycin and the reconsolidation hypothesis: J.W. Rudy, et al.; Learn. Mem. 13, 1 (2006), Review Abstract
A chemical screen identifies anisomycin as an anoikis sensitizer that functions by decreasing FLIP protein synthesis: I.A. Mawji, et al.; Cancer Res. 67, 8307 (2007) Abstract
Anisomycin abrogates repression of protooncogene c-fos transcription in E1A + cHa-ras-transformed cells through activation of MEK/ERK kinase cascade: A. N. Kukushkin, et al.; J. Cell. Biochem. 103, 1005 (2008) Abstract
 
 
ALX-201-116 Revised 21-Jan-08
Annexin V (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V/Related Products
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ALX-201-116-C100   100 µg 194.00 USD Add To Cart
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Product Specification
MW: ~52kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. Recombinant human annexin V fused to GST.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Affinity purified.
FORMULATION: Lyophilized. Contains PBS, pH 7.4, and 0.02% sodium azide.
RECONSTITUTION: Reconstitute with 100µl sterile water.
SPECIFICITY: Binds to phosphatidylserine (PS).
BIOLOGICAL ACTIVITY: Exhibits anti-phospholipase activity.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Reconstituted solutions can be kept at 4°C under sterile conditions for one week or should be frozen in aliquots at -20°C. Repeated freeze/thaw cycles (>3) may result in an adverse effect on the activity of annexin V.
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-209-257 Revised 18-May-06
Annexin V (mouse) (recombinant) (Alexa Fluor® 488)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V/Related Products
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ALX-209-257-T100   100 tests 245.00 USD Add To Cart
Product Specification
QUANTITY: 100 tests/500µl.
FORMULATION: Liquid. Contains 0.02% sodium azide.
SPECIFICITY: Binds phosphatidylserine (PS) residues.
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light. Do not freeze.
Product Images
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Product Description
Alexa Fluor® 488-labelled recombinant mouse annexin shows bright green fluorescence (Ex(max): 495nm; Em(max): 519nm).
Product Specific Literature References