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NF-kB Pathway
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ALX-210-929 Revised 16-Apr-08
Polyclonal Antibody to Cardif (human) (AT107)
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SYNONYMS anti-CARD Adapter Inducing Interferon-β (human) PAb (AT107)
anti-IPS-1 (human) PAb (AT107)
anti-Interferon-β Promoter Stimulator Protein 1 (human) PAb (AT107)
anti-MAVS (human) PAb (AT107)
anti-Mitochondrial Antiviral Signalling Protein (human) PAb (AT107)
anti-VISA (human) PAb (AT107)
anti-Virus-induced Signalling Adapter (human) PAb (AT107)
PRODUCT LINE Immunology
PRODUCT CATEGORY Antiviral Signalling/Related Products
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ALX-210-929-C100   100 µg 370.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
SPECIFICITY: Recognizes human cardif.
APPLICATION: Immunocytochemistry (1:500)
Immunoprecipitation (1:100)
Western Blot (1:2’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
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Product Specific Literature References
Regulation of antiviral responses by a direct and specific interaction between TRAF3 and Cardif : S. K. Saha; EMBO J. 25, 3257 (2006) Abstract
Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008) Abstract
General Information
RIG-I (retinoic acid-inducible gene I; Ddx58) and Mda5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons.
Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and Mda5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005) Abstract
CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006) Abstract
Further Categories Containing This Product:
NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins/Related Products • Polyclonal Antibodies
 
 
ALX-210-903 Revised 25-Jan-08
Polyclonal Antibody to CARMA1 (AL220)
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SYNONYMS anti-CARD-containing MAGUK Protein 1 PAb (AL220)
anti-CARD11 PAb (AL220)
anti-Caspase Recruitment Domain-containnig Protein 11 PAb (AL220)
anti-Bimp3 PAb (AL220)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Pathway Other Products
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ALX-210-903-R100   100 µl 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum.
IMMUNOGEN: N-terminal human CARMA1 (CARD-containing MAGUK protein 1) (aa 1-221 containing the CARD domain).
SPECIFICITY: Recognizes human and mouse CARMA1. Detects a band of ~130Dka by Western blot.
APPLICATION: Immunocytochemistry
Immunohistochemistry
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-606.
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Product Specific Literature References
CARMA1 is a critical lipid raft-associated regulator of TCR-induced NF-kappaB activation: O. Gaide, et al.; Nat. Immunol. 3, 836 (2002) Abstract
Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1: R.B. Medeiros, et al.; Science 316, 754 (2007) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Carma1, a CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappaB activation: O. Gaide, et al.; FEBS Lett. 496, 121 (2001) Abstract
A requirement for CARMA1 in TCR-induced NF-kappa B activation: D. Wang, et al.; Nat. Immunol. 3, 830 (2002) Abstract
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins/Related Products • Polyclonal Antibodies
 
 
ALX-210-804 Revised 14-Jul-08
Polyclonal Antibody to Caspase-1
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SYNONYMS anti-Interleukin-1β Converting Enzyme PAb
anti-IL-1β Converting Enzyme PAb
anti-ICE PAb
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Antibodies
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ALX-210-804-C100   100 µg 315.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.2mg/ml.
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS, pH 7.2, containing 50% glycerol, 1% BSA and 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 129-152 of human caspase-1.
SPECIFICITY: Recognizes human, mouse and rat caspase-1. Detects bands of ~45kDa (procaspase-1) and ~20kDa (cleavage product) by Western blot.
APPLICATION: Immunocytochemistry (10-20µg/ml)
Immunoprecipitation (4-8µg/ml)
Western Blot (1-4µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
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Further Categories Containing This Product:
Inflammasome • Polyclonal Antibodies
 
 
ALX-210-809 Revised 19-May-08
Polyclonal Antibody to Caspase-5
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Antibodies
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ALX-210-809-C100   100 µg 273.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Monkey
Others
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.2mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS, pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
IMMUNOGEN: Synthetic peptide surrounding aa 162 of human caspase-5.
SPECIFICITY: Recognizes human, mouse, rat, hamster, monkey, rabbit and bovine caspase-5. Preferentially detects the intermediate (32kDa) and the fully cleaved (20kDa) caspase-5 by Western blot.
APPLICATION: Western Blot (0.5-4µg/ml).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
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Further Categories Containing This Product:
Inflammasome • Polyclonal Antibodies
 
 
ALX-210-910 Revised 29-Feb-08
Polyclonal Antibody to CYLD (human) (AF888)
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SYNONYMS anti-Cylindromatosis Tumor Suppressor Protein (human) PAb (AF888)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Pathway Other Products
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ALX-210-910-C050   50 µg 195.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.45mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 1% BSA and 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 56-70 (G56HSRIPSAKGKKNQI70) of human CYLD (cylindromatosis tumor suppressor protein).
SPECIFICITY: Recognizes human CYLD. Detects a band of ~120kDa by Western blot.
APPLICATION: Immunocytochemistry (on transfected cells; 1:200)
Western Blot (on transfected cells; 1:3'000).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
The tumor suppressor CYLD Acts as a Negative Regulator for Toll-like Receptor 2 Signaling via Negative Cross-talk with TRAF6 and TRAF7: H. Yoshida, et al.; J. Biol. Chem. 280, 41111 (2005) Abstract; Full Text
General Information
Cylindromas are benign adnexal skin tumors caused by germ line mutations in the CYLD gene. In most cases the second wild type allele is lost in tumor tissue suggesting that CYLD functions as tumor-suppressor. CYLD is a protein of 956 amino acids harboring a functional deubiquitinating domain at the C-terminal end. CYLD was shown to interact with TRAF2, NEMO and TRIP (Trompouki et al.; Brummelkamp et al.; Kovalenko et al.; Regamey et al.). Furthermore, CYLD is a negative regulator of NF-κB activation mediated by TNF family members and IL-1β. Downregulation of NF-κB activity depends on the deubiquitinating activity of CYLD which removes lysine-63 linked ubiquitin chains from target proteins. These findings indicate that cylindromas arise through constitutive NF-κB activation leading to hyperproliferation and tumor growth.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
CYLD is a deubiquitinating enzyme that negatively regulates NF-kappaB activation by TNFR family members: E. Trompouki, et al.; Nature 424, 793 (2003) Abstract
Loss of the cylindromatosis tumour suppressor inhibits apoptosis by activating NF-kappaB: T.R. Brummelkamp, et al.; Nature 424, 797 (2003) Abstract
The tumour suppressor CYLD negatively regulates NF-kappaB signalling by deubiquitination: A. Kovalenko, et al.; Nature 424, 801 (2003) Abstract
The tumor suppressor CYLD interacts with TRIP and regulates negatively nuclear factor kappaB activation by tumor necrosis factor: A. Regamey, et al.; J. Exp. Med. 198, 1959 (2003) Abstract
Further Categories Containing This Product:
Deubiquitination Other Products • Polyclonal Antibodies • Tumor Suppressors/Related Products
 
 
ALX-210-928 Revised 08-Nov-07
Polyclonal Antibody to LRRK2 (human) (AT106)
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SYNONYMS anti-Leucine-rich Repeat Kinase 2 (human) PAb (AT106)
anti-RIP7 (human) PAb (AT106)
anti-PARK8 (human) PAb (AT106)
anti-Dardarin (human) PAb (AT106)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY RIP Kinases
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ALX-210-928-C100   100 µg 350.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human LRRK2 (leucine-rich repeat kinase 2) (aa 1838-2133).
SPECIFICITY: Recognizes human LRRK2. The strongest recognized epitope is PDQPRLTIPISQ (aa 1843-1854) as defined by epitope mapping.
APPLICATION: Immunohistochemistry (paraffin sections (1:500))
Immunoprecipitation (1:100)
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
LRRK2 in Parkinson’s disease and dementia with Lewy bodies: X. Zhu, et al.; Mol. Neurodegener. 1, 17 (2006) Abstract; Full Text
General Information
LRRK2 is a large protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named Roc, and a kinase domain that is closely related to the RIP kinase domain. LRRK2 gene is expressed in brain as well as in other tissues such as lung, liver and heart. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with parkinsonism. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Cloning of the gene containing mutations that cause PARK8-linked Parkinson’s disease: C. Paisan-Ruiz, et al.; Neuron 44, 595 (2004) Abstract
From The Cover: Parkinson’s disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity: A.B. West, et al.; PNAS 102, 16842 (2005) Abstract
The RIP kinases: crucial integrators of cellular stress: E. Meylan and J. Tschopp; TIBS 30, 151 (2005) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies • Parkinson's Disease Other Products
 
 
ALX-210-935 Revised 20-Feb-08
Polyclonal Antibody to MDA5 (human) (AT113)
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SYNONYMS anti-Melanoma Differentiation-associated Gene 5 (human) PAb (AT113)
anti-Helicard (human) PAb (AT113)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases/Related Products
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ALX-210-935-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).
SPECIFICITY: Recognizes human MDA5.
APPLICATION: Immunoprecipitation (1:100)
Western Blot (1:1‘000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008) Abstract
General Information
MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract
Further Categories Containing This Product:
NF-kB Pathway Other Products • Interferons & Interferon Receptors Other Products • Caspase Recruitment Domain [CARD] Proteins/Related Products • Antiviral Signalling/Related Products • Polyclonal Antibodies
 
 
ALX-210-352 Revised 30-Oct-06
Polyclonal Antibody to MDA5 (mouse) (AL180)
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SYNONYMS anti-Melanoma Differentiation-associated Gene 5 (mouse) PAb (AL180)
anti-Helicard (mouse) PAb (AL180)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-352-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Contains 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse MDA5 (melanoma differentiation-associated gene 5) (aa 2-208).
SPECIFICITY: Recognizes mouse MDA5.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Overexpression of Helicard, a CARD-Containing Helicase Cleaved during Apoptosis, Accelerates DNA Degradation: M. Kovacsovics, et al.; Curr. Biol. 12, 838 (2002) Abstract
General Information
MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002) Abstract
Further Categories Containing This Product:
NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins/Related Products • Antiviral Signalling/Related Products • Interferons & Interferon Receptors Other Products • Polyclonal Antibodies
 
 
ALX-210-958 Revised 07-May-08 New product
Polyclonal Antibody to NALP12 (human) (AL236)
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SYNONYMS anti-NLRP12 PAb (human) (AL236)
anti-NACHT-, LRR- and PYD-containing Protein 12 PAb (human) (AL236)
anti-PYRIN-containing APAF1-like Protein 7 PAb (human) (AL236)
anti-Monarch-1 PAb (human) (AL236)
anti-Regulated by Nitric Oxide PAb (human) (AL236)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Inflammasome
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-958-C100   100 µg 350.00 USD Add To Cart
Product Specification
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.5mg/ml
PURITY: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa near the N-terminus of human NALP12.
SPECIFICITY: Recognizes human NALP12.
APPLICATION: Immunoprecipitation (1:200)
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
MANUFACTURER Manufactured by Apotech Corpora