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Signal Transduction
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ALX-850-066 Revised 23-Apr-08
CD30L, Soluble (human) (recombinant) Set
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SYNONYMS CD153, Soluble (human) (recombinant) Set
TNFSF 8, Soluble (human) (recombinant) Set
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD30 & CD30L / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-066-KI01   1 Set 460.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human CD30L (CD153) (aa 63-234) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG®-tag.
CONCENTRATION: CD30L: 0.1mg/ml after reconstitution.
Enhancer: 1mg/ml after reconstitution.
KIT/SET CONTAINS: 10µg of CD30L, Soluble (human) (recombinant) (Prod. No. ALX-522-028) and 2x50µg Enhancer for Ligands (Prod. No. ALX-804-034).
PURITY: ≥95% (SDS-PAGE).
FORMULATION: Lyophilized. Contains PBS.
RECONSTITUTION: Reconstitute CD30L with 100µl and each vial of enhancer with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
SPECIFICITY: CD30L binds to human CD30 (Ki-1).
APPLICATION: Can be used together with anti-FLAG® MAb to detect membrane-bound human CD30. Co-stimulates T cells in the presence of cross-linking enhancer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
Selective enrichment of CD30-expressing cells within the blast region of lymphocytes from patients with primary HIV infection (PHI): P. Biswas, et al.; J. Biol. Regul. Homeost. Agents 16, 33 (2002) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Receptor / Ligand + Enhancer Set • CD Kits & Sets
 
 
ALX-522-094 Revised 27-Mar-08
CD30L, Soluble (mouse) (recombinant)
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SYNONYMS CD153, Soluble (mouse) (recombinant)
TNFSF8, Soluble (mouse) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD30 & CD30L / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-094-C010   10 µg 365.00 USD Add To Cart
Product Specification
MW: ~42kDa (SDS-PAGE)
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse CD30L (CD153) (aa 71-239) is fused at the N-terminus to a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile distilled water. The resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.
SPECIFICITY: Binds to human and mouse CD30.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
General Information
The CD30 ligand is a member of the tumor necrosis factor (TNF) family. CD30L is a type II, transmembrane protein expressed on T and B cells, monocytes/macrophages, neutrophils, megakaryocytes, erythroid precursors and eosinophils. After interaction with its receptor CD30, CD30L may induce TNF receptor-associated factor 1 (TRAF1), TRAF2, 3 and 5 recruitment. CD30-CD30L have been reported to be involved in costimulation of T cell proliferation, induction of HIV expression, negative selection and apoptosis as well as in diabetes and several allergic, viral and autoimmune diseases.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P32972: CD30L (mouse)
FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
OX40 ligand and CD30 ligand are expressed on adult but not neonatal CD4+CD3- inducer cells: evidence that IL-7 signals regulate CD30 ligand but not OX40 ligand expression: M.Y. Kim, et al.; J. Immunol. 174, 6686 (2005) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • CD Proteins
 
 
ALX-522-064 Revised 05-Apr-06
CD40 (human):COMP (human) (recombinant)
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SYNONYMS TNFRSF 5 (human):COMP (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
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ALX-522-064-C010   10 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~36kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human CD40 (aa 1-193) is fused to a FLAG®-tag at the N-terminus and to human cartilage oligomeric matrix protein (COMP, aa 32-80) at the C-terminus.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds human CD40L (CD154).
BIOLOGICAL ACTIVITY: Potent blocker of mouse CD40L-dependent B cell proliferation without the requirement of any crosslinking reagent (enhancer).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
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Product Description
First non-antibody blocker of CD40L signalling
Product Specific Literature References
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000) Abstract
General Information
COMP (Cartilage Oligomeric Matrix Protein) also designated thrombospondin 5 (TSP 5) is an oligomeric protein synthesized by chondrocytes in all type of cartilage. It belongs to the thrombospondin family of extracellular proteins. It is a pentameric molecule forming a structure similar to a bouquet of flowers. It may have storage and delivery function for hydrophobic cell-signalling molecules such as vitamin D.
COMP is a calcium-binding protein composed of 5 identical disulfide-linked subunits of 755 amino acids each with a high percentage of acidic residues (30%). The molecular weight of the homopentamer is 524 kDa, as determined by sedimentation equilibrium ultracentrifugation. Each subunit is composed of an N-terminal coiled-coil domain (unique to COMP), four epidermal growth factor (EGF)-type repeats, followed by eight calmodulin-like repeats and a globular C-terminal domain. The C-terminal domain binds to collagens I, II and IX.
Chimeras composed of proteins fused to the pentamerization domain of COMP (aa 32-75) show an increased avidity compared to the protein alone.
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-522-016 Revised 28-Apr-06
CD40 (human):Fc (human) (recombinant)
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SYNONYMS TNFRSF 5 (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
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ALX-522-016-C050   50 µg 355.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~54kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The cysteine-rich region (aa 21-193) of human CD40 is fused to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds human and mouse CD40L (CD154).
APPLICATION: Can be used together with anti-human IgG1 PAb to detect membrane-bound human or mouse CD40L. CD40:Fc should be used at concentrations between 1 and 100µg/ml depending on the level of CD40L surface expression.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P25942: CD40 (human) (precursor)
AfCS Signalling Gateway link A000031: CD40 (mouse)
MANUFACTURER

Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-850-262 Revised 11-Apr-08
CD40, Soluble (human) ELISA Kit
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SYNONYMS TNFSF 5, Soluble (human) ELISA Kit
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
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ALX-850-262-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 6.92pg/ml (range 7.8 to 500 pg/ml)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human soluble CD40 in cell culture supernatants, serum and other body fluids.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
CD Kits & Sets • ELISA & EIA Kits
 
 
ALX-522-076 Revised 19-Apr-06
Fc (human):CD40L, Soluble (human) (recombinant)
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SYNONYMS Fc (human):CD154, Soluble (human) (recombinant)
Fc (human):TNFSF 5, Soluble (human) (recombinant)
Fc (human):gp39, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
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ALX-522-076-C010   10 µg 400.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~58kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa).
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; BioWhittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human CD40.
BIOLOGICAL ACTIVITY: Stimulates the proliferation of human dendritic cells. Note: Does not require an enhancer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • CD Proteins
 
 
ALX-522-072 Revised 19-Apr-06
Fc (human):CD40L, Soluble (mouse) (recombinant)
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SYNONYMS Fc (human):CD154, Soluble (mouse) (recombinant)
Fc (human):TNFSF 5, Soluble (mouse) (recombinant)
Fc (human):gp39, Soluble (mouse) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
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ALX-522-072-C010   10 µg 400.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~58kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa).
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human and mouse CD40.
BIOLOGICAL ACTIVITY: Stimulates the proliferation of mouse B cells and dendritic cells. Note: Does not require an enhancer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-522-015 Revised 28-Apr-06
CD40L, Soluble (human) (recombinant)
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SYNONYMS CD154, Soluble (human) (recombinant)
TNFSF 5, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-015-2010   2x10 µg 200.00 USD Add To Cart
ALX-522-015-6010 SuperPack 6x10 µg 410.00 USD Add To Cart
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Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~18kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute each tube with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human CD40.
BIOLOGICAL ACTIVITY: Stimulates growth of B cells. The activity of rhsCD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of cross-linking enhancer (see Set Prod. No. ALX-850-064). For stimulation of mouse cells via CD40 use CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070 or ALX-850-075).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
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Product Specific Literature References
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 12051 (1998) Abstract; Full Text
CD45 inhibits CD40L-induced microglial activation via negative regulation of the Src/p44/42 MAPK pathway: J. Tan, et al.; J. Biol. Chem. 275, 37224 (2000) Abstract; Full Text
Histamine polarizes human dendritic cells into Th2 cell-promoting effector dendritic cells: G. Caron, et al.; J. Immunol. 167, 3682 (2001) Abstract
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001) Abstract; Full Text
Synergy between CD40 ligation and IL-4 on fibroblast proliferation involves IL-4 receptor signaling: S.P. Atamas, et al.; J. Immunol. 168, 1139 (2002) Abstract
Pro-inflammatory effect of TWEAK/Fn14 interaction on human umbilical vein endothelial cells: N. Harada, et al.; BBRC 299, 488 (2002) Abstract
Suppression of IL-12 Production by Soluble CD40 Ligand: Evidence for Involvement of the p44/42 Mitogen-Activated Protein Kinase Pathway: M. Wittmann, et al.; J. Immunol. 168, 3793 (2002) Abstract
HIV-1 Nef intersects the macrophage CD40L signalling pathway to promote resting-cell infection: S. Swingler, et al.; Nature 424, 213 (2003) Full Text
Platelet-Activating Factor Mediates CD40-Dependent Angiogenesis and Endothelial-Smooth Muscle Cell Interaction: S. Russo, et al.; J. Immunol. 171, 5489 (2003) Abstract
Induction of Cell Cycle Arrest and Apoptosis by the Proteasome Inhibitor PS-341 in Hodgkin Disease Cell Lines Is Independent of Inhibitor of Nuclear Factor-kappaB Mutations or Activation of the CD30, CD40, and RANK Receptors: B. Zheng, et al.; Clin. Cancer Res. 10, 3207 (2004) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P29965: CD40L (human)
AfCS Signalling Gateway link A000536: CD40L (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-850-064 Revised 23-Apr-08
CD40L, Soluble (human) (recombinant) Set
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SYNONYMS TNFSF 5, Soluble (human) (recombinant) Set
CD154, Soluble (human) (recombinant) Set
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-064-KI01   1 Set 440.00 USD