• Home
  • Sitemap
  • Help
  • Technical Support
  • Contact
Signal Transduction
You are here: Product Lines > Signal Transduction
 
Toolbar - View Selection
 
 Items 220-240 of 2948 Page 12 of 148 Select Page: << 1 2 3 4 5 6 7 8 9 10  >>  
ALX-201-101 Revised 04-Mar-08
MMP-15 (Catalytic Domain) (human) (recombinant)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMPs
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-101-C010   10 µg 560.00 USD Add To Cart
Product Specification
MW: ~20kDa
EC: 3.4.24.-
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 0.2mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM NaCl, 5mM CaCl2, 50mM imidazol and 0.05% Brji-35.
SPECIFIC ACTIVITY: ≥30mU/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1µmol Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 per min. at 37°C, pH 7.5.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for several weeks when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

Measurement of catalytic activity

1 Preparation and stability of solutions:
· Peptide hydrolysis buffer: 50mM TRIS-HCl, pH 7.5, 150mM NaCl, 5mM CaCl2, 0.025% Brij 35. Solution is stable for several weeks at 4°C.
· Stock solution of peptide substrate: 100µM solution of Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg in 20% DMSO. Store at -20°C.
· Stock solution of unquenched peptide: 10µM solution of (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-NH2 (Mca-Pro-Leu) in 20% DMSO. Store at -20°C.

2 Assay protocol:
The activity of MMP-15 is measured fluorimetrically with a synthetic internally quenched fluorescent substrate according to Knight et al.
An excitation wavelength of 328nm and an emission wavelength of 393nm are set in an appropriate fluorimeter. The instrument is calibrated with the unquenched peptide Mca-Pro-Leu at a concentration corresponding to between 2 and 10% hydrolysis of the protease substrate. Kinetic reactions are conveniently carried out in a constant volume of 2.5ml. The substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg is diluted in peptide hydrolysis buffer to a concentration of 0.8µM and equilibrated at a temperature of 37°C. Aliquots of 2 to 4µl of the activation mixture are than added and the increase in fluorescence is recorded over a time interval between 2 and 12 minutes. Activity units per ml enzyme solution are calculated according to the following equation:

Activity (U/ml) = (CMca-Pro-Leu/FMca-Pro-Leu) x (ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg/Venzyme) x Vtotal

CMca-Pro-Leu: Concentration of Mca-Pro-Leu used for calibration of the fluorimeter (µmoles/ml).
FMca-Pro-Leu: Fluorescence of Mca-Pro-Leu at the concentration CMca-Pro-Leu used for fluorimeter calibration.
ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg: Change in fluorescence during peptide hydrolysis per min.
Vtotal: Volume of peptide hydrolysis reaction (2.5ml).
Venzyme: Volume of added enzyme (0.002 to 0.004ml).

3 Inhibitors:
The catalytic domain of MMP-15 is inhibited by tissue inhibitors of MMP-2 and -3 (TIMP-2 and TIMP-3) and by chelators of divalent cations like EDTA or o-phenanthroline.

Swiss-Prot link P51511: MMP-15 (human) (precursor)
AfCS Signalling Gateway link A001467: MMP-15 (mouse)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-103 Revised 04-Mar-08
MMP-16 (Catalytic Domain) (human) (recombinant)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMPs
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-103-C010   10 µg 560.00 USD Add To Cart
Product Specification
EC: 3.4.24.-
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 0.2mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5  containing 150mM NaCl, 5mM CaCl2, 1% glycerol and 0.1% Triton X-100.
SPECIFIC ACTIVITY: ≥75mU/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1µmol Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 per min. at 37°C, pH 7.5.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
Product Specific Literature References
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

Measurement of catalytic activity

1 Preparation and stability of solutions:
· Peptide hydrolysis buffer: 50mM TRIS-HCl, pH 7.5, 150mM NaCl, 5mM CaCl2, 0.025% Brij 35. Solution is stable for several weeks at 4°C.
· Stock solution of peptide substrate: 100µM solution of Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg in 20% DMSO. Store at -20°C.
· Stock solution of unquenched peptide: 10µM solution of (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-NH2 (Mca-Pro-Leu) in 20% DMSO. Store at -20°C.

2 Assay protocol:
The activity of MMP-16 is measured fluorimetrically with a synthetic internally quenched fluorescent substrate according to Knight et al.
An excitation wavelength of 328nm and an emission wavelength of 393nm are set in an appropriate fluorimeter. The instrument is calibrated with the unquenched peptide Mca-Pro-Leu at a concentration corresponding to between 2 and 10% hydrolysis of the protease substrate. Kinetic reactions are conveniently carried out in a constant volume of 2.5ml. The substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg is diluted in peptide hydrolysis buffer to a concentration of 0.8µM and equilibrated at a temperature of 37°C. Aliquots of 2 to 4µl of the activation mixture are than added and the increase in fluorescence is recorded over a time interval between 2 and 12 minutes. Activity units per ml enzyme solution are calculated according to the following equation:

Activity (U/ml) = (ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg x  CMca-Pro-Leu x Vtotal) / (1000 x FMca-Pro-Leu x Venzyme)
CMca-Pro-Leu: Concentration of Mca-Pro-Leu used for calibration of the fluorimeter (µmoles/ml).
FMca-Pro-Leu: Fluorescence of Mca-Pro-Leu at the concentration CMca-Pro-Leu used for fluorimeter calibration.
ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg: Change in fluorescence during peptide hydrolysis per min.
Vtotal: Volume of peptide hydrolysis reaction (2.5ml).
Venzyme: Volume of added enzyme (0.002 to 0.004ml).


3 Inhibitors:
The catalytic domain of MMP-16 is inhibited by tissue inhibitors of MMP-2 (TIMP-2) and by chelators of divalent cations like EDTA or o-phenanthroline.

Swiss-Prot link P51512: MMP-16 (human) (precursor)
AfCS Siganlling Gateway link A001468: MMP-16 (mouse)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-104 Revised 04-Mar-08
MMP-17 (Catalytic Domain) (human) (recombinant) (His)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMPs
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-104-C010   10 µg 560.00 USD Add To Cart
Product Specification
EC: 3.4.24.-
SOURCE/HOST: Produced in E. coli. Fused at the C-terminus to a His-tag.
CONCENTRATION: 0.2mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 50mM TRIS-HCl, pH 7.5 containing 150mM NaCl, 5mM CaCl2, 1% glycerol and 0.1% Triton X-100.
SPECIFIC ACTIVITY: ≥6mU/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1µmol Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 per min. at 37°C, pH 7.5.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
Product Description
The catalytic domain of human MMP-17 is produced by tryptic activation of a recombinant soluble proform.
Product Specific Literature References
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

 Measurement of catalytic activity

1 Preparation and stability of solutions:
· Peptide hydrolysis buffer: 50mM TRIS-HCl, pH 7.5, 150mM NaCl, 5mM CaCl2, 0.025% Brij 35. Solution is stable for several weeks at 4°C.
· Stock solution of peptide substrate: 100µM solution of Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg in 20% DMSO. Store at -20°C.
· Stock solution of unquenched peptide: 10µM solution of (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-NH2 (Mca-Pro-Leu) in 20% DMSO. Store at -20°C.

2 Assay protocol:
The activity of MMP-17 is measured fluorimetrically with a synthetic internally quenched fluorescent substrate according to Knight et al.
An excitation wavelength of 328nm and an emission wavelength of 393nm are set in an appropriate fluorimeter. The instrument is calibrated with the unquenched peptide Mca-Pro-Leu at a concentration corresponding to between 2 and 10% hydrolysis of the protease substrate (0.016-0.08μM). Kinetic reactions are conveniently carried out in a constant volume of 2.5ml. The substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg is diluted in peptide hydrolysis buffer to a concentration of 0.8µM and equilibrated at a temperature of 37°C. Aliquots of 8 to 10µl of the activation mixture are than added and the increase in fluorescence is recorded over a time interval between 2 and 15 minutes. Activity units per ml enzyme solution are calculated according to the following equation:

Activity (U/ml) = (CMca-Pro-Leu/FMca-Pro-Leu) x (ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg/Venzyme) x Vtotal

CMca-Pro-Leu: Concentration of Mca-Pro-Leu used for calibration of the fluorimeter (µmoles/ml).
FMca-Pro-Leu: Fluorescence of Mca-Pro-Leu at the concentration CMca-Pro-Leu used for fluorimeter calibration.
ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg: Change in fluorescence during peptide hydrolysis per min.
Vtotal: Volume of peptide hydrolysis reaction (2.5ml).
Venzyme: Volume of added enzyme (0.002 to 0.004ml).


3 Inhibitors:
The catalytic domain of MMP-17 is inhibited by tissue inhibitors of MMP-1 (TIMP-1) and by chelators of divalent cations like EDTA.


Swiss-Prot link Q9ULZ9: MMP-17 (human) (precursor)
AfCS Signalling Gateway link A001469: MMP-17 (mouse)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-105 Revised 21-Oct-08
MMP-24 (Catalytic Domain) (human) (recombinant) (His)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMPs
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-105-C010   10 µg 560.00 USD Add To Cart
Product Specification
MW: ~23kDa
EC: 3.4.24.-
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 0.2mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM NaCl and 5mM CaCl2.
SPECIFIC ACTIVITY: ≥120mU/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1µmol Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 per min. at 37°C, pH 7.5.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
Product Description
Activation generates two molecular species. One species begins at the N-terminus with Tyr156 of full-length MMP-24, while the other species is two aa residues shorter and starts with Leu158. Both species extend to Ser351 of MMP-24 and contain an additional His-tag sequence at the C-terminus.
Product Specific Literature References
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

Measurement of catalytic activity

1 Preparation and stability of solutions:
· Peptide hydrolysis buffer: 50mM TRIS-HCl, pH 7.5, 150mM NaCl, 5mM CaCl2, 0.025% Brij 35. Solution is stable for several weeks at 4°C.
· Stock solution of peptide substrate: 100µM solution of Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg in 20% DMSO. Store at -20°C.
· Stock solution of unquenched peptide: 10µM solution of (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-NH2 (Mca-Pro-Leu) in 20% DMSO. Store at -20°C.

2 Assay protocol:
The activity of MMP-24 is measured fluorimetrically with a synthetic internally quenched fluorescent substrate according to Knight et al.
An excitation wavelength of 328nm and an emission wavelength of 393nm are set in an appropriate fluorimeter. The instrument is calibrated with the unquenched peptide Mca-Pro-Leu at a concentration corresponding to between 2 and 10% hydrolysis of the protease substrate. Kinetic reactions are conveniently carried out in a constant volume of 2.5ml. The substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg is diluted in peptide hydrolysis buffer to a concentration of 0.8µM and equilibrated at a temperature of 37°C. Aliquots of 2 to 4µl of the activation mixture are than added and the increase in fluorescence is recorded over a time interval between 2 and 12 minutes. Activity units per ml enzyme solution are calculated according to the following equation:

 Activity (U/ml) = (CMca-Pro-Leu/FMca-Pro-Leu) x (ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg/Venzyme) x Vtotal

 CMca-Pro-Leu: Concentration of Mca-Pro-Leu used for calibration of the fluorimeter (µmoles/ml).
 FMca-Pro-Leu: Fluorescence of Mca-Pro-Leu at the concentration CMca-Pro-Leu used for fluorimeter calibration.
 ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg: Change in fluorescence during peptide hydrolysis per min.

 Vtotal: Volume of peptide hydrolysis reaction (2.5ml).
 Venzyme: Volume of added enzyme (0.002 to 0.004ml).

3 Inhibitors:
The catalytic domain of MMP-24 is inhibited by tissue inhibitors of MMP-2 (TIMP-2) and by chelators of divalent cations like EDTA or o-phenanthroline.

Swiss-Prot link Q9Y5R2: MMP-24 (human) (precursor)
AfCS Signalling Gateway link A001474: MMP-24 (mouse)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-112 Revised 18-Mar-08
Granzyme B (human) (recombinant)
Add to Clipboard
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Granzymes
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-112-C010   10 µg 310.00 USD Add To Cart
Product Specification
MW: ~25.5kDa.
EC: 3.4.21.79
SOURCE/HOST: Produced in E. coli. Active human granzyme B.
CONCENTRATION: 0.2mg/ml
FORMULATION: Liquid. In PBS containing 10% glycerol. Contains no preservatives.
SPECIFIC ACTIVITY: ≥7.0U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol Ac-IEPD-pNA (Prod. No. ALX-260-152) per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl and 0.01% Triton X-100.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Dilute solutions (e. g. 1-1000ng/ml) should be used within 24 hours.
HANDLING: For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Crystal structure of the caspase activator human granzyme B, a proteinase highly specific for an Asp-P1 residue: E. Estebanez-Perpina, et al.; Biol. Chem. 381, 1203 (2000) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P10144: Granzyme B (human) (precursor)
General Literature References
Definition and redesign of the extended substrate specificity of granzyme B  : J.L. Harris, et al.; J. Biol. Chem. 273, 27364 (1998) Abstract; Full Text
Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma  : E. Wilharm, et al.; J. Biol. Chem. 274, 27331 (1999) Abstract; Full Text
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-115 Revised 07-Nov-08
SuperKillerTRAIL™, Soluble (human) (recombinant)
Add to Clipboard
SYNONYMS Apo-2L, Soluble (human) (recombinant) (CC-mutant)
TNFSF 10, Soluble (human) (recombinant) (CC-mutant)
CD253, Soluble (human) (recombinant) (CC-mutant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY TRAIL [Apo-2L]
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-115-C010   10 µg 360.00 USD Add To Cart
ALX-201-115-3010 SuperPack 3x10 µg 720.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.
CONCENTRATION: 0.5mg/ml.
PURITY: ≥98% (SDS-PAGE)
PURITY DETAIL: Affinity purified. Sterile filtered.
FORMULATION: Liquid. In 20mM HEPES, pH 7.4, containing 300mM NaCl, 0.01% Tween 20, 1% sucrose, 1mM DTT.
ENDOTOXIN CONTENT: <0.02EU/µg purified protein (LAL test; Bio Whittaker).
SPECIFICITY: Binds to human TRAIL receptors.
BIOLOGICAL ACTIVITY: Induces apoptosis at concentrations of >1ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. Shows superior activity on human cell lines that require extensive cross-linking of TRAIL-Rs for killing (e.g. Jurkat).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C. In order to retain full activity don’t dilute higher than to a concentration of 0.1mg/ml, prepare 20µl aliquots and store at -80°C. For a special storage and dilution buffer see Prod. No. ALX-505-005. Working dilution: 2-100ng/ml.
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Synthetic lethal targeting of MYC by activation of the DR5 death receptor pathway: Y. Wang, et al.; Cancer Cell 5, 501 (2004) Abstract
Sulforaphane targets pancreatic tumor-initiating cells by NF-{kappa}B-induced anti-apoptotic signaling: G. Kallifatidis, et al.; Gut Epub ahead of print, (2008) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P50591: TRAIL (human)
AfCS Signalling Gateway link A002862: TRAIL (mouse)
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-116 Revised 21-Jan-08
Annexin V (human) (recombinant)
Add to Clipboard
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-116-C100   100 µg 194.00 USD Add To Cart
Inquire
Product Specification
MW: ~52kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. Recombinant human annexin V fused to GST.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Affinity purified.
FORMULATION: Lyophilized. Contains PBS, pH 7.4, and 0.02% sodium azide.
RECONSTITUTION: Reconstitute with 100µl sterile water.
SPECIFICITY: Binds to phosphatidylserine (PS).
BIOLOGICAL ACTIVITY: Exhibits anti-phospholipase activity.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Reconstituted solutions can be kept at 4°C under sterile conditions for one week or should be frozen in aliquots at -20°C. Repeated freeze/thaw cycles (>3) may result in an adverse effect on the activity of annexin V.
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-117 Revised 13-Dec-04
Granzyme K (human) (recombinant)
Add to Clipboard
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Granzymes
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-117-C010   10 µg 310.00 USD Add To Cart
Product Specification
MW: ~26kDa.
EC: 3.4.21.-
SOURCE/HOST: Produced in E. coli. Active human granzyme K.
CONCENTRATION: 0.2mg/ml
FORMULATION: Liquid. Recombinant protein in PBS containing glycerol. Does not contain any preservatives.
SPECIFIC ACTIVITY: ~29U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 6 months when stored at -80°C. Dilute solutions (e. g. 1-1000ng/ml) should be used within 24 hours.
HANDLING: For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma: E. Wilharm, et al.; J. Biol. Chem. 274, 27331 (1999) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P49863: Granzyme K (human) (precursor)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-118 Revised 23-May-07
Granzyme A (human) (recombinant)
Add to Clipboard
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Granzymes
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-118-C010   10 µg 310.00 USD Add To Cart
Product Specification
MW: ~52kDa (homodimer).
EC: 3.4.21.78
SOURCE/HOST: Produced in E. coli. Active human granzyme A.
CONCENTRATION: 0.2mg/ml.
FORMULATION: Liquid. In 0.05M MES, pH 6.0, containing 10% glycerol and ~0.52M sodium chloride. Contains no preservatives.
SPECIFIC ACTIVITY: ~110U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -80°C. Do not dilute to more than 0.2mg/ml. Do not store dilute solutions (1-1000ng/ml) for more than 24 hours.
HANDLING: Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P12544: Granzyme A (human) (precursor)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins