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Phospho-specific Antibodies
You are here: Product Lines > Signal Transduction > PI(3)K-Akt-mTOR Pathway > Phospho-specific Antibodies
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ALX-803-303 Revised 18-Sep-07
Monoclonal Antibody to Akt (19G7)
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SYNONYMS anti-PKB MAb (19G7)
anti-Protein Kinase B MAb (19G7)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Akt [PKB] / Related Products
Ordering Information
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ALX-803-303-C100   100 µg 240.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 19G7
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.2mg/ml
PURITY: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
PURITY DETAIL: Protein A-affinity purified.
IMMUNOGEN: Recombinant mouse Akt (protein kinase B; PKB).
SPECIFICITY: Recognizes human, mouse, rat, dog and hamster Akt. Detects Akt1 (PKB-α) and Akt2 (PKB-β) strong than Akt3 (PKB-γ). Detects also phosphorylated forms of Akt.
APPLICATION: ELISA
Immunoprecipitation (5µg per 700µl protein extract)
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
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Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-401 Revised 28-Mar-08
Monoclonal Antibody to Akt (phosphorylated) (pSer473) (11E6)
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SYNONYMS anti-PKB (phosphorylated) (pSer473) MAb (11E6)
anti-Protein Kinase B (phosphorylated) (pSer473) MAb (11E6)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Akt [PKB] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-401-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 11E6
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to C-terminal (PQFpSYSA) of human Akt (protein kinase B; PKB) phosphorylated at Ser473.
SPECIFICITY: Recognizes human, mouse, rat and dog Ser472/473/474-phosphorylated Akt (see technical note). Does not cross-react with dephosphorylated Akt or other unrelated serine-phosphorylated proteins.
APPLICATION: Flow Cytometry
Immunohistochemistry
Western Blot: (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL: Included (Prod. No. ALX-840-027).
General Information
BACKGROUND/TECHNICAL INFORMATION Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBα), Akt2 (PKBβ), and Akt3 (PKBγ). Akt1 shares 81% and 83% aa identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. The phosphorylation site slightly differs, thus the phosphorylation site Ser473 of Akt1 corresponds to Ser474 in Akt2 and Ser472 in Akt3.

Akt1:  V461DSERRPHFPQFS473YSASSTA480       Swiss-Prot-Link: P31749
Akt2:  L461LELDQRTHFPQFS474YSASIRE481       Swiss-Prot-Link: P31751
Akt3:  D461NERRPHFPQFS472YSASGRE479        Swiss-Prot-Link: Q9Y243
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-802-009 Revised 31-Oct-06
Monoclonal Antibody to Ca2+/Calmodulin-dependent Protein Kinase II (rat) (α Subunit) (phosphorylated) (pThr286) (22B1)
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SYNONYMS anti-CAM Kinase II (rat) (α Subunit) (phosphorylated) (pThr286) MAb (22B1)
anti-CAMK2 (rat) (α Subunit) (phosphorylated) (pThr286) MAb (22B1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY CAM Kinase Antibodies
Ordering Information
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ALX-802-009-R100   100 µl 385.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
CLONE: 22B1
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 281-294 (M281HRQET(PSO3)VDCLKKFN294) of the α-subunit of mouse and rat CaM kinase II (Ca2+/Calmodulin-dependent Protein Kinase II) thiophosphorylated at (Thr286).
SPECIFICITY: Recognizes recombinant and native mouse and rat phosphorylated α-subunit of rat CaM kinase II.
APPLICATION: ELISA
Immunocytochemistry (1:100)
Immunoprecipitation
Western Blot (1:2'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- and dephosphokinase with complementary phosphorylation site-specific antibodies: B.L. Patton, et al.; Mol. Biol. Cell 4, 159 (1993) Abstract
Targeted genomic disruption of H-ras and N-ras, individually or in combination, reveals the dispensability of both loci for mouse growth and development: L.M. Esteban, et al.; Mol. Cell. Biol. 21, 1444 (2001) Abstract; Full Text
Cytoplasmic polyadenylation element binding protein-dependent protein synthesis is regulated by calcium/calmodulin-dependent protein kinase II: C.M. Atkins, et al.; J. Neurosci. 24, 5193 (2004) Abstract; Full Text
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-399 Revised 01-Dec-05
Monoclonal Antibody to cAMP-dependent Protein Kinase Substrate (phosphorylated) (15B12)
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SYNONYMS anti-PKA Substrate (phosphorylated) MAb (15B12)
anti-Protein Kinase A Substrate (phosphorylated) MAb (15B12)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKA Antibodies
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ALX-804-399-C100   100 µg Inquire
Product Specification
SPECIES CROSSREACTIVITY:
Others
CLONE: 15B12
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN: Synthetic phosphopeptide corresponding to PKA (protein kinase A) substrate consensus phoshorylation site of activated PKA substrate.
SPECIFICITY: Recognizes RRXpS motif of activated PKA substrate in a broad range of species.
APPLICATION: ELISA (non-radioactive quantification of phospho-peptides in kinase/phosphatase assays; use at 0.1-1µg/ml).
Note: Not suitable for Western blot.
Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-388 Revised 20-Sep-07
Monoclonal Antibody to Casein Kinase II Substrate (phosphorylated) (9F4)
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SYNONYMS anti-CKII Substrate (phosphorylated) MAb (9F4)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Casein Kinase Substrates
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ALX-804-388-C100   100 µg Inquire
Product Specification
SPECIES CROSSREACTIVITY:
Others
CLONE: 9F4
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic phosphopeptide corresponding to CKII (casein kinase II) substrate consensus phosphorylation site of activated CKII substrate.
SPECIFICITY: Recognizes DpSD motif of activated CKII substrate in a broad range of species.
APPLICATION: ELISA (non-radioactive quantification of phospho-peptides in kinase/phosphatase assays; use at 0.1-1µg/ml).
Note: Not suitable for Western blot!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-402 Revised 07-Nov-06
Monoclonal Antibody to cGMP-activated Protein Kinase Substrate (phosphorylated) (7H1)
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SYNONYMS anti-Protein Kinase G Substrate (phosphorylated) MAb (7H1)
anti-PKG Substrate (phosphorylated) MAb (7H1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKG Substrates
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ALX-804-402-C100   100 µg Inquire
Product Specification
SPECIES CROSSREACTIVITY:
Others
CLONE: 7H1
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 2x PBS, containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min, RT).
IMMUNOGEN: Synthetic phosphopeptide corresponding to PKG (protein kinase G) substrate consensus phosphorylation site of activated PKG substrate.
SPECIFICITY: Recognizes VpSK motif of activated PKG substrate in a broad range of species.
APPLICATION: ELISA (non-radioactive quantification of phospho-peptides in kinase/phosphatase assays; use at 0.1-1µg/ml).
Note: Not suitable for Western blot!
Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Phospho-specific Antibodies • Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products • Monoclonal Antibodies
 
 
ALX-804-389 Revised 30-May-08
Monoclonal Antibody to CREB (pSer133) and ATF1 (pSer63) (phosphorylated) (10E9)
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PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY CREB & CREB Binding Protein / Related Products
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ALX-804-389-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 10E9
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to aa 125-135 of CREB phosphorylated at Ser133.
SPECIFICITY: Recognizes the RRPpSYRK motif of human, mouse or dog CREB phosphorylated at Ser133 and ATF1 phosphorylated at Ser63. Detects bands of ~43kDa (CREB) and ~38kDa (ATF1) by Western blot.
APPLICATION: Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED: yes
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-390 Revised 05-Jun-07
Monoclonal Antibody to EGF Receptor (phosphorylated) (pSer1047) (1H9)
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SYNONYMS anti-Epidermal Growth Factor Receptor (phosphorylated) (pSer1047) MAb (1H9)
anti-EGFR (phosphorylated) (pSer1047) MAb (1H9)
anti-HER-1 (phosphorylated) (pSer1047) MAb (1H9)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY EGFR Kinase Antibodies
Ordering Information
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ALX-804-390-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 1H9
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min, RT).
IMMUNOGEN: Synthetic peptide corresponding to a portion of human EGF receptor (epidermal growth factor receptor) phosphorylated at Ser1047.
SPECIFICITY: Recognizes human, mouse and dog Ser1047-phosphorylated EGF receptor. Does not cross-react with unphosphorylated EGF receptor or other unrelated serine-phosphorylated proteins.
APPLICATION: Immunocytochemistry (0.1-1µg/ml)
Luminex
Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL: Included (Prod. No. ALX-840-025).
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-160 Revised 20-Sep-07
Monoclonal Antibody to EGF Receptor (phosphorylated) (Tyr1173) (9H2)
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SYNONYMS anti-Epidermal Growth Factor Receptor (phosphorylated) (Tyr1173) MAb (9H2)
anti-EGFR (phosphorylated) (Tyr1173) MAb (9H2)
anti-HER-1 (phosphorylated) (Tyr1173) MAb (9H2)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY EGFR Kinase Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-160-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 9H2
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min, RT).
IMMUNOGEN: Synthetic peptide corresponding to a portion of human EGF receptor (epidermal growth factor receptor) phosphorylated at Tyr1173.
SPECIFICITY: Recognizes the major autophosphorylation site N1170AEpYLRV of human, mouse and dog EGF receptor. Does not cross-react with the non-phosphorylated human, mouse, rat and dog EGF receptor or with unrelated Tyr-phosphorylated proteins. Non-activated EGF receptor (dephosphorylated at Tyr1173) can be detected in parallel using Prod. No. ALX-804-161 that specifically recognizes the non-phosphorylated peptide motif.
APPLICATION: Immunocytochemistry (0.1-1µg/ml)
Immunohistochemistry (paraffin sections, frozen sections)
Immunoprecipitation
Luminex
Western Blot: (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED: yes
Product Images
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Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-061 Revised 24-Jun-08
Monoclonal Antibody to ERK1/2 (phosphorylated) (12D4)
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SYNONYMS anti-MAPK2/3 (phosphorylated) MAb (12D4)
anti-MAP Kinase 2/3 (phosphorylated) MAb (12D4)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY ERK1 & ERK2 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-061-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 12D4
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Peptide comprising the pThr-Glu-pTyr motif in activated ERK1 and ERK2.
SPECIFICITY: Recognizes the pThr-Glu-pTyr motif of activated human, mouse, rat and dog ERK1 and ERK2. Requires phosphorylation both at the threonine and the tyrosine site and does not cross-react with the non-phosphorylated form of the protein. Does not cross-react with activated JNK or p38.
APPLICATION: Immunocytochemistry (1-10µg/ml)
Immunohistochemistry
Immunoprecipitation (1-10µg per 106 pervanadate-treated A431 or HepG2 cells)
Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL: Included (Prod. No. ALX-840-025).
Product Specific Literature References
IL-4-induced priming of human intestinal mast cells for enhanced survival and Th2 cytokine generation is reversible and associated with increased activity of ERK1/2 and c-Fos: A. Lorentz, et al.; J. Immunol. 174, 6751 (2005) Abstract; Full Text
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-374 Revised 14-Feb-06
Monoclonal Antibody to GSK-3β (phosphorylated) (pSer9) (2D3)
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SYNONYMS anti-Glycogen Synthase Kinase-3β (phosphorylated) (pSer9) MAb (2D3)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY GSK-3 Antibodies