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Phosphatases / Related Products
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ALX-804-636 Revised 31-Oct-06
Monoclonal Antibody to PP2A (Subunit A) (PR65) (α Isoform) (4C10)
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SYNONYMS anti-Protein Phosphatase 2A (Subunit A) (PR65) (α Isoform) MAb (4C10)
anti-PP2A A/PR65α MAb (4C10)
anti-Medium Tumor Antigen-associated 61kDa Protein MAb (4C10)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PP2A / Related Products
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ALX-804-636-C050   50 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 4C10
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human PP2A (subunit A) (PR65) (α isoform) (aa 1-168).
SPECIFICITY: Recognizes human and mouse PP2A (subunit A) (PR65) (α isoform). Recognizes mouse PP2A (subunit A) (PR65) (β isoform) (weak).
CROSSREACTIVITY PROTEINS: PP2A (subunit A) (PR65) (β isoform) (weak)
APPLICATION: Western Blot (1:5’000)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
General Information
The regulatory A subunit (PR65) is tightly associated with catalytic subunit (PP2Ac), forming a scaffold to which the appropriate B subunit can bind. Different B subunits interact via the same or overlapping sites within the A subunit of the core dimer, which explains why binding of the B subunits is mutually exclusive. Two distinct PR65 isoforms (α and β) which share 86% sequence identity are ubiquitiously expressed. Recent studies reported that the genes encoding both the α and β isoforms of PR65/A were altered at low frequency in a variety of primary human tumors, identifying these subunits as tumorsuppressors. Partial or total loss (by cleavage through caspase-3) of the PR65/A subunit resulted in an increase in PP2A activity towards specific pro-apoptotic substrates involved in the cellular apoptotic response.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-569 Revised 24-Oct-06
Monoclonal Antibody to PP2A (Subunit B) (PR55) (α Isoform) (2G9)
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SYNONYMS anti-Protein Phosphatase 2A (Subunit B) (PR55) (α Isoform) MAb (2G9)
anti-PP2A B/PR55α MAb (2G9)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PP2A / Related Products
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ALX-804-569-C100   100 µg 330.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 2G9
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 398-411 (C398ASGKRKKDEISVD411) of rat PP2A (subunit B) (PR55) (α isoform). This sequence is completely conserved in human, mouse, rat, pig, rabbit, chicken and xenopus.
SPECIFICITY: Recognizes human, mouse and rat PP2A (subunit B) (PR55) (α isoform). Cross-reactivity with other species has not been tested. Detects a band of ~50kDa by Western blot. Cross-reacts with mouse and rat PP2A (subunit B) (PR55) (δ isoform).
CROSSREACTIVITY PROTEINS: PP2A (subunit B) (PR55) (δ isoform)
APPLICATION: Western Blot (1:1'000-1:10'000)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Protein phosphatase 2A associates with and regulates atypical PKC and the epithelial tight junction complex: V. Nunbhakdi-Craig, et al.; J. Cell Biol. 158, 967 (2002) Abstract; Full Text
Identification of a subunit of a novel Kleisin-beta/SMC complex as a potential substrate of protein phosphatase 2A: F.M. Yeong, et al.; Curr. Biol. 13, 2058 (2003) Abstract
General Literature References
Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling: V. Janssens and J. Goris; Biochem J. 353, 417 (2001) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-570 Revised 29-Nov-04
Monoclonal Antibody to PPM1 (mouse) (4A4)
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SYNONYMS anti-Protein Phosphatase Methyltransferase 1 (mouse) MAb (4A4)
anti-LCMT1 (mouse) MAb (4A4)
anti-Leucine Carboxyl Methyltransferase 1 (mouse) MAb (4A4)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Phosphatases Other Products
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ALX-804-570-C100   100 µg 330.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: 4A4
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
FORMULATION: Liquid. Protein G purified antibody in PBS, containing 0.02% sodium azide, PEG and sucrose.
IMMUNOGEN: Recombinant mouse PPM1 (protein phosphatase methyltransferase 1).
SPECIFICITY: Recognizes mouse PPM1.
APPLICATION: Western Blot (1:1'000-1:10'000)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-254 Revised 14-Aug-07
Monoclonal Antibody to PTEN (6H2.1)
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SYNONYMS anti-Phosphatase and Tensin Homolog Deleted on Chromosome 10 MAb (6H2.1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PTEN / Related Products
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ALX-804-254-C100   100 µg 480.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 6H2.1
ISOTYPE: Mouse IgG
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Protein A-affinity purified. Sterile filtered.
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Recombinant human PTEN.
SPECIFICITY: Recognizes human, mouse and rat PTEN (epitope aa 304-403). Detects a band of ~60kDa by Western blot.
APPLICATION: ELISA
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation (5µg)
Western Blot (1-5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
POSITIVE CONTROL: For Positive Control in Western blot see Prod. No. ALX-201-079.
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Product Specific Literature References
Immunohistochemical evidence of loss of PTEN expression in primary ductal adenocarcinomas of the breast: A. Perren, et al.; Am. J. Pathol. 155, 1253 (1999) Abstract; Full Text
PTEN suppresses breast cancer cell growth by phosphatase activity- dependent G1 arrest followed by cell death: L.P. Weng, et al.; Cancer Res. 59, 5808 (1999) Abstract; Full Text
Altered PTEN expression as a diagnostic marker for the earliest endometrial precancers: G.L. Mutter, et al.; J. Natl. Cancer Inst. 92, 924 (2000) Abstract
Changes in endometrial PTEN expression throughout the human menstrual cycle: G.L. Mutter, et al.; J. Clin. Endocrinol. Metab. 85, 2334 (2000) Abstract; Full Text
Molecular identification of latent precancers in histologically normal endometrium: G.L. Mutter, et al.; Cancer Res. 61, 4311 (2001) Abstract; Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products • Monoclonal Antibodies
 
 
ALX-804-451 Revised 14-Aug-07
Monoclonal Antibody to PTEN (11G8.1)
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SYNONYMS anti-Phosphatase and Tensin Homolog Deleted on Chromosome 10 MAb (11G8.1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PTEN / Related Products
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ALX-804-451-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 11G8.1
ISOTYPE: Mouse IgG
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Protein A purified antibody, sterile filtered in PBS.
IMMUNOGEN: Recombinant human PTEN.
SPECIFICITY: Recognizes human and mouse PTEN (epitope aa 152-200). Detects a band of ~60 kDa by Western blot.
APPLICATION: Immunoprecipitation (5µg/sample)
Western Blot (1-5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
POSITIVE CONTROL: For Positive Control in Western blot see Prod. No. ALX-201-079.
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Further Categories Containing This Product:
Tumor Suppressors / Related Products • Monoclonal Antibodies
 
 
ALX-804-533 Revised 21-Oct-08
Monoclonal Antibody to TRAP5 (human) (TRAP-01)
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SYNONYMS anti-Tartrate-resistant Acid Phosphatase 5 (human) MAb (TRAP-01)
anti-TRACP (human) MAb (TRAP-01)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY TRAP5 [TRACP]
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ALX-804-533-C100   100 µg 508.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: TRAP-01
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: Protein G-affinity purified. Sterile filtered.
FORMULATION: Lyophilized from PBS, pH 7.4.
RECONSTITUTION: Reconstitute with 100µl of distilled water. Activity is not affected by slight turbidity. Clarify by centrifugation.
IMMUNOGEN: Recombinant human TRAP5 (tartrate-resistant acid phosphatase 5).
SPECIFICITY: Recognizes human TRAP5.
APPLICATION: ELISA
ImmunoCapture Enzyme-Activity Assay
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Specific Literature References
[1] Purification and N-terminal sequence of two tartrate-resistant acid phosphatases type-5 from the hairy cell leukemia spleen: J.J. Stepan, et al.; BBRC 165, 1027 (1989) Abstract
[2] Tartrate-resistant acid phosphatase 5b: a novel serum marker of bone resorption: J.M. Halleen, et al.; J. Bone Miner. Res 15, 1337 (2000) Abstract
[3] Tartrate-resistant acid phosphatase isoform 5b as serum marker for osteoclastic activity: A.J. Janckila, et al.; Clin. Chem. 47, 74 (2001) Abstract
[4] Clinical performance of immunoreactive tartrate-resistant acid phosphatase isoform 5b as a marker of bone resorption: R.A. Hannon, et al.; Bone 34, 187 (2004) Abstract
[5] TRACP as an osteopontin phosphatase: G. Andersson, et al.; J. Bone Miner. Res 18, 1912 (2003) Abstract
General Information
TRAP5b is claimed to be a specific marker of bone resorption [2-4]. Both osteopontin and bone sialoprotein are colocalized with TRAP5 in the resorption lacuna of osteoclasts and dephosphorylation of osteopontin impair its ability to promote adhesion as well as migration of osteoclasts in vitro [5].
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-803-057 Revised 31-Oct-06
Monoclonal Antibody to Tyrosine Phosphatase IA-2 (Intracellular Domain) (605-772) (97/4D9)
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SYNONYMS anti-PTP IA-2ic (605-772) MAb (97/4D9)
anti-Islet Cell Antigen 512 (Intracellular Domain) (605-772) MAb (97/4D9)
anti-Islet Cell Autoantigen 3 (Intracellular Domain) (605-772) MAb (97/4D9)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Protein Tyrosine Phosphatases Other Products
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ALX-803-057-C100   100 µg 300.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 97/4D9
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Ammonium sulphate precipitation.
FORMULATION: Liquid. In PBS containing 0.9mg/ml sodium azide.
IMMUNOGEN: Recombinant human IA-2ic (tyrosine phosphatase IA-2 (intracellular domain)).
SPECIFICITY: Recognizes an epitope (aa 605-772) of human, mouse and rat IA-2ic.
APPLICATION: Immunohistochemistry (frozen sections)
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-803-056 Revised 31-Oct-06
Monoclonal Antibody to Tyrosine Phosphatase IA-2 (Intracellular Domain) (618-628) (98/4H6)
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SYNONYMS anti-PTP IA-2ic (618-628) MAb (98/4H6)
anti-Islet Cell Antigen 512 (Intracellular Domain) (618-628) MAb (98/4H6)
anti-Islet Cell Autoantigen 3 (Intracellular Domain) (618-628) MAb (98/4H6)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Protein Tyrosine Phosphatases Other Products
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ALX-803-056-C100   100 µg 300.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 98/4H6
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Ammonium sulphate precipitation.
FORMULATION: Liquid. In PBS containing 0.9mg/ml sodium azide.
IMMUNOGEN: Recombinant human IA-2ic (tyrosine phosphatase IA-2 (intracellular domain)).
SPECIFICITY: Recognizes an epitope (aa 618-628) of human, mouse and rat IA-2ic.
APPLICATION: Immunohistochemistry (frozen sections)
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-803-058 Revised 31-Oct-06
Monoclonal Antibody to Tyrosine Phosphatase IA-2 (Intracellular Domain) (771-979) (103/2C4)
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SYNONYMS anti-PTP IA-2ic (771-979) MAb (103/2C4)
anti-Islet Cell Antigen 512 (Intracellular Domain) (771-979) MAb (103/2C4)
anti-Islet Cell Autoantigen 3 (Intracellular Domain) (771-979) MAb (103/2C4)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Protein Tyrosine Phosphatases Other Products
Ordering Information
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ALX-803-058-C100   100 µg 300.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 103/2C4
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Ammonium sulphate precipitation.
FORMULATION: Liquid. In PBS containing 0.9mg/ml sodium azide.
IMMUNOGEN: Recombinant human IA-2ic (tyrosine phosphatase IA-2 (intracellular domain)).
SPECIFICITY: Recognizes an epitope (aa 771-979) of human, mouse and rat IA-2ic.
APPLICATION: Immunohistochemistry (frozen sections)
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-350-061 Revised 08-Oct-08
Nodularin
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Microcystins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-061-C050   50 µg 37.00 USD Add To Cart
ALX-350-061-C100   100 µg 70.00 USD Add To Cart
ALX-350-061-C250   250 µg 155.00 USD Add To Cart
ALX-350-061-M001   1 mg 530.00 USD Add To Cart
Product Specification
FORMULA: C41H60N8O10
MW: 825.0
CAS NUMBER: 118399-22-7
RTECS: GU2294250
SOURCE/HOST: Isolated from Nodularia spumigena.
PURITY: ≥95% (HPLC)
APPEARANCE: Dry residue containing traces of monobasic potassium phosphate.
SOLUBILITY: Soluble in methanol:water (1:1).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: TOXIC.

Product Description
Inhibitor of protein phosphatase 1 (PP1) (IC50=1.8nM), protein phosphatase 2A (PP2A) (IC50=0.026nM) and to a lesser extent protein phosphatase 2B (PP2B) (IC50=8.7µm). Similar to microcystin-LR (Prod. No. ALX-350-012) but with increased water solubility. Genotoxic. Hepatoxic. Induces oxidative DNA damage by oxidation of purines. Induces apoptosis in HepG2 cells.
Product Specific Literature References
Nodularin, microcystin and the configuration of Adda: K.L. Rinehart, et al.; JACS 110, 8557 (1988)
Toxicity and partial structure of a hepatotoxic peptide produced by the cyanobacterium Nodularia spumigena Mertens emend. L575 from New Zealand: W.W. Carmichael, et al.; Appl. Environ. Microbiol. 54, 2257 (1988) Abstract
Inhibition of protein phosphatases by microcystins and nodularin associated with hepatotoxicity: S. Yoshizawa, et al.; J. Cancer Res. Clin. Oncol. 116, 609 (1990) Abstract
Rapid purification of the peptide toxins microcystin-LR and nodularin: C. Martin, et al.; FEMS Microbiol. Lett. 56, 1 (1990) Abstract
In vitro and in vivo effects of protein phosphatase inhibitors, microcystins and nodularin, on mouse skin and fibroblasts: R. Matsushima, et al.; BBRC 171, 867 (1990) Abstract
Internal surface reversed-phase high-performance liquid chromatographic separation of the cyanobacterial peptide toxins microcystin-LA, -LR, -YR, -RR and nodularin: J.A. Meriluoto, et al.; J. Chromatogr. 509, 390 (1990) Abstract
Cyanobacterial nodularin is a potent inhibitor of type 1 and type 2A protein phosphatases: R.E. Honkanen, et al.; Mol. Pharmacol. 40, 577 (1991) Abstract
Degradation of the cyanobacterial hepatotoxin, nodularin, under light and dark conditions: H. Twist & G.A. Codd; FEMS Microbiol. Lett. 151, 83 (1997) Abstract
Isolation and detection of microcystins and nodularins, cyanobacterial peptide hepatotoxins: J. Meriluoto, et al.; Methods Mol. Biol. 145, 65 (2000)