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Cell Death / Apoptosis / Autophagy
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ALX-151-031 Revised 23-Nov-06
Caspase-3 Control Peptide
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Inhibitors
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-151-031-M001   1 mg 35.00 USD Add To Cart
ALX-151-031-M005   5 mg 120.00 USD Add To Cart
Product Specification
SEQUENCE: Ac-Asp-Glu-Val-Asp-OH
FORMULA: C20H30N4O12
MW: 518.5
PURITY: ≥97%
APPEARANCE: Lyophilized.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Peptides
 
 
ALX-850-216 Revised 24-Jun-08
Caspase-3 Fluorometric Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-216-KI01   25 tests 175.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl DEVD-AFC (1mM), 100µl DTT (1M).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light. Store Lysis Buffer and 2X Reaction Buffer at 4°C after opening.
Product Description
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate DEVD-AFC.
Product Specific Literature References
Apoptosis and Id2 expression in diaphragm and soleus muscle from the emphysematous hamster: H. Degens, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R135 (2007) Abstract
General Information
DEVD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-3 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Activity Assays
 
 
ALX-201-093 Revised 15-Apr-08
Caspase-4 (active) (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-093-U025   25 U 203.00 USD Add To Cart
ALX-201-093-U100   100 U 371.00 USD Add To Cart
Product Specification
EC: 3.4.22.57
SOURCE/HOST: Produced in E. coli.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute with PBS containing 15% glycerol to 1U/µl.
SPECIFIC ACTIVITY: ~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION: Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
Caspase-4 is a member of the caspase family of cysteine proteases. It exists in cells as an inactive pro-enzyme. The pro-enzyme is matured by proteolysis to yield large and small subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. The expressed caspase-4 spontaneously undergoes auto-processing to yield subunits characteristic of the native enzyme. The active caspase-4 is routinely tested for its ability to enzymatically cleave the substrate WEHD-AFC or WEHD-pNA.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P49662: Caspase-4 (human) (precursor)
AfCS Signalling Gateway link A000499: Caspase-4 (mouse)
Further Categories Containing This Product:
Enzymes • Endoplasmatic Reticulum Stress • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-094 Revised 03-May-06
Caspase-5 (active) (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-094-U025   25 U 203.00 USD Add To Cart
ALX-201-094-U100   100 U 371.00 USD Add To Cart
Product Specification
EC: 3.4.22.-
SOURCE/HOST: Produced in E. coli.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY: ~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION: In combination with caspase-5 activity assays, the enzyme is useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P51878: Caspase-5 (human) (precursor)
AfCS Signalling Gateway link A000500: Caspase-5 (mouse)
Further Categories Containing This Product:
Inflammasome • Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-850-217 Revised 24-Jan-05
Caspase-5 Colorimetric Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-217-KI01   25 tests 203.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl WEHD-pNA Substrate (4mM), 100µl DTT (1M), 25ml Dilution Buffer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening.
Product Description
Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate WEHD-pNA.
General Information
The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Inflammasome • Activity Assays
 
 
ALX-850-218 Revised 24-Jan-05
Caspase-5 Fluorometric Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-218-KI01   25 tests 189.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl WEHD-AFC Substrate (1mM), 100µl DTT (1M).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening.
Product Description
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate WEHD-AFC.
General Information
WEHD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-5 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Inflammasome • Activity Assays
 
 
ALX-201-039 Revised 24-Jan-05
Caspase-6 (active) (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-039-C005   5 µg 255.00 USD Add To Cart
Product Specification
EC: 3.4.22.-
SOURCE/HOST: Produced in E. coli.
FORMULATION: Liquid. 5µg in 25µl of 50mM TRIS (pH 8.0) containing 100mM sodium chloride and 50mM imidazole.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least one week when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
[1] Biochemical characteristics of caspases-3, -6, -7 and -8: H.R. Stennicke & G.S. Salvesen; J. Biol. Chem. 272, 25719 (1997) Abstract; Full Text
General Information
When expressed in E. coli, caspase-6 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme.
The rate of caspase-6 enzymatic hydrolysis can be measured by the release of AMC from the caspase substrate Ac-DEVD-AMC (Prod. No. ALX-260-031) as emission at 440nm and excitation at 380nm using a spectrofluorometer [1].
BACKGROUND/TECHNICAL INFORMATION
Enzyme Evaluation Protocol
This protocol is used to measure caspase enzyme activity [1]. The synthetic fluorogenic peptide Ac-DEVD-AMC (Prod. No. ALX-260-031) is used as the caspase enzyme substrate. The enzyme cleaves the substrate releasing the fluorescent AMC (7-amino-4-methylcoumarin). AMC release can be measured by spectrofluorometry using UV.

1. Add 20mM of Ac-DEVD-AMC (Prod. No. ALX-260-031) to 1ml of assay buffer (20mM PIPES, 100mM sodium chloride, 10mM DTT, 1mM EDTA, 0.1% (w/v) CHAPS, 10% sucrose, pH 7.2). Add the appropriate amount of  active caspase to the mixture (50ng/ml).
2. Incubate for 1 hour at 37°C.
3. Measure the AMC liberated from the Ac-DEVD-AMC using a spectrofluorometer with an excitation wavelength of 380nm and an emission wavelength of 440nm.

Note: Enzyme activity can also be measured using the caspase enzyme substrate Ac-DEVD-pNA (Prod. No. ALX-260-033) at 37°C in a spectromax at 405nm wavelength.

Swiss-Prot link P55212: Caspase-6 (human) (precursor)
AfCS Signalling Gateway link A000501: Caspase-6 (mouse)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-060 Revised 02-Jun-08
Caspase-6 (active) (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-060-U025   25 U 203.00 USD Add To Cart
ALX-201-060-U100   100 U 371.00 USD Add To Cart
Product Specification
EC: 3.4.22.59
SOURCE/HOST: Produced in E. coli.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute to 1U/µl with PBS containing 15% glycerol.
BIOLOGICAL ACTIVITY: Cleaves substrates exhibiting consensus sequence VEID, e.g. Ac-VEID-AFC (Prod. No. ALX-260-111), Ac-VEID-AMC (Prod. No. ALX-260-064) and Ac-VEID-pNA (Prod. No. ALX-260-063).
SPECIFIC ACTIVITY: ~13'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate VEID-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION: Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1U per assay for analyzing caspase activity.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
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General Information
Caspase-6 (also known as Mch2) is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Same as other caspases, caspase-6 also exists in cells as an inactive proenzyme. During apoptosis procaspase-6 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed form of caspase-6 consists of large (18kDa) and small (11kDa) subunits which associate to form the active enzyme. The active caspase-6 has been shown involving in the proteolysis of poly(ADP-ribose) polymerase (PARP), an enzyme that is involved in DNA repair and genomic maintenance.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P55212: Caspase-6 (human) (precursor)
AfCS Signalling Gateway link A000501: Caspase-6 (mouse)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-850-219 Revised 22-Apr-08
Caspase-6 Colorimetric Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-219-KI01   25 tests 189.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS:

25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl VEID-pNA Substrate (4mM), 100µl DTT (1M), 25ml Dilution buffer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light. Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening.
Product Description
Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate VEID-pNA.
General Information
The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-6 activity.
MANUFACTURER

Manufactured by BioVision.
Further Categories Containing This Product:
Activity Assays
 
 
ALX-850-220 Revised 22-Apr-08
Caspase-6 Fluorometric Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-220-KI01   25 tests 175.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl VEID-AFC Substrate (1mM), 100µl DTT (1M).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light. Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening.
Product Description
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VEID-AFC.
General Information
VEID-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-6 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-6 activity.
MANUFACTURER

Manufactured by BioVision.
Further Categories Containing This Product:
Activity Assays
 
 
ALX-201-040 Revised 24-Jan-05
Caspase-7 (active) (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-040-C005   5 µg 255.00 USD Add To Cart
Product Specification
EC: 3.4.22.-
SOURCE/HOST: Produced in E. coli.
FORMULATION: Liquid. 5µg in 25µl of 50mM TRIS (pH 8.0) containing 100mM sodium chloride and 50mM imidazole.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable at least one week when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
[1] Biochemical characteristics of caspases-3, -6, -7, and -8: H.R. Stennicke & G.S. Salvesen; J. Biol. Chem. 272, 25719 (1997) Abstract; Full Text
General Information
When expressed in E. coli, caspase-7 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme.
The rate of caspase-7 enzymatic hydrolysis can be measured by the release of AMC from the caspase substrate Ac-DEVD-AMC (Prod. No. ALX-260-031) as emission at 440nm and excitation at 380nm using a spectrofluorometer [1].
BACKGROUND/TECHNICAL INFORMATION
Enzym