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Adaptor Proteins for Death Receptors / Related Products
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ALX-748-017 Revised 24-Mar-05
FLIP (human) cDNA Probe
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FLIP
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ALX-748-017-C001   1 µg 140.00 USD Add To Cart
Product Specification
SOURCE/HOST: Derived from the coding region of the human FLIPL gene. Has a length of ~700 bp located within the region between nucleotides 1-700.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (agarose gel electrophoresis)
FORMULATION: Lyophilized powder.
RECONSTITUTION: Reconstitute with 10µl sterile water or 10mM Tris.HCl containing 0.1mM Na2EDTA.
APPLICATION: Northern Blot. Human FLIP mRNA migrates as a 4.7kb, 3.9kb and 2.2kb species.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
DNA & RNA Probes / Related Products
 
 
ALX-840-601 Revised 06-Dec-07
Positive Control (Cell Lysate) for Antibodies to FLIP (human)
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SYNONYMS Positive Control (Cell Lysate) for Antibodies to CASPER (human)
Positive Control (Cell Lysate) for Antibodies to CLARP (human)
Positive Control (Cell Lysate) for Antibodies to I-FLICE (human)
Positive Control (Cell Lysate) for Antibodies to Usurpin (human)
PRODUCT LINE Immunology
PRODUCT CATEGORY Positive Controls (Cell Lysates & Extracts)
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ALX-840-601-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human FLIP (aa 1-480) fused at the C-terminus to a HA-tag.
QUANTITY: 100µl corresponding to 2x106 cells.
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for MAb to FLIP (Dave-2) (Prod. No. ALX-804-127) and MAb to FLIP (human) (NF6) (Prod. No. ALX-804-428).
Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
FLIP
 
 
ALX-804-127 Revised 21-Oct-08
Monoclonal Antibody to FLIP (Dave-2)
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SYNONYMS anti-CASPER MAb (Dave-2)
anti-CLARP MAb (Dave-2)
anti-I-FLICE MAb (Dave-2)
anti-Usurpin MAb (Dave-2)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FLIP
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ALX-804-127-C100   100 µg 370.00 USD Add To Cart
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Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Dave-2
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN: Recombinant human FLIP (aa 1-480).
SPECIFICITY: Recognizes an epitope (aa 1-200) present in both short (FLIPs) and long (FLIPL) splice variants of human and mouse FLIP.
APPLICATION: Immunoprecipitation
Western Blot
Note: Not recommended for immunohistochemistry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-601.
Product Specific Literature References
Fas engagement induces the maturation of dendritic cells (DCs), the release of interleukin (IL)-1beta, and the production of interferon gamma in the absence of IL-12 during DC-T cell cognate interaction: a new role for Fas ligand in inf: M. Rescigno, et al.; J. Exp. Med. 192, 1661 (2000) Abstract; Full Text
Protein kinase C regulates FADD recruitment and death-inducing signaling complex formation in Fas/CD95-induced apoptosis: M. Gomez-Angelats & J.A. Cidlowski; J. Biol. Chem. 276, 44944 (2001) Abstract; Full Text
Fas aggregation does not correlate with Fas-mediated apoptosis: Y. Lee & E. Shacter; J. Immunol. 167, 82 (2001) Abstract
NF-kB signals induce the expression of c-FLIP: O. Micheau, et al.; Mol. Cell. Biol. 21, 5299 (2001) Abstract; Full Text
Fas-associated death domain protein (FADD) and caspase-8 mediate up-regulation of c-Fos by Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via a FLICE inhibitory protein (FLIP)-regulated pathway: D. Siegmund, et al.; J. Biol. Chem. 276, 32585 (2001) Abstract; Full Text
Characterization of the human FLICE-inhibitory protein locus and comparison of the anti-apoptotic activity of four different flip isoforms: M. Djerbi, et al.; Scand. J. Immunol. 54, 180 (2002) Abstract
c-FLIP efficiently rescues TRAF-2-/- cells from TNF-induced apoptosis: C. Guiet, et al.; Cell Death Differ. 9, 138 (2002) Abstract
The caspase 8 inhibitor c-FLIP(L) modulates T-cell receptor-induced proliferation but not activation-induced cell death of lymphocytes: S.M. Lens, et al.; Mol. Cell. Biol. 22, 5419 (2002) Abstract
Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Signaling in Activated T Cells Abrogates TRAIL-Induced Apoptosis Upstream of the Mitochondrial Amplification Loop and Caspase-8: T.S. Soderstrom, et al.; J. Immunol. 169, 2851 (2002) Abstract
Expression of the long form of human FLIP by retroviral gene transfer of hemopoietic stem cells exacerbates experimental autoimmune encephalomyelitis: M. Djerbi, et al.; J. Immunol. 170, 2064 (2003) Abstract; Full Text
Bile acids stimulate cFLIP phosphorylation enhancing TRAIL-mediated apoptosis: H. Higuchi, et al.; J. Biol. Chem. 278, 454 (2003) Abstract; Full Text
Chemotherapy enhances TNF-related apoptosis-inducing ligand DISC assembly in HT29 human colon cancer cells: S. Lacour, et al.; Oncogene 22, 1807 (2003) Abstract
Fas-associated protein with death domain (FADD)-independent recruitment of c-FLIPL to death receptor 5: T.G. Jin, et al.; J. Biol. Chem. 279, 55594 (2004) Abstract; Full Text
Targeting Bcl-x(L) in esophageal squamous cancer to sensitize to chemotherapy plus TRAIL-induced apoptosis while normal epithelial cells are protected by blockade of caspase 9: K. Kim, et al.; Cell Death Differ. 11, 583 (2004) Abstract
The anti-apoptotic factor Bcl-2 can functionally substitute for the B cell survival but not for the marginal zone B cell differentiation activity of BAFF: A. Tardivel, et al.; Eur. J. Immunol. 34, 509 (2004) Abstract
The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover: L. Chang, et al.; Cell 124, 601 (2006) Abstract
A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-α-induced Apoptosis: V. Oliveira, et al.; J. Biol. Chem. 283, 13174 (2008) Abstract; Full Text
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MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Inhibition of death receptor signals by cellular FLIP: M. Irmler, et al.; Nature 388, 190 (1997) Abstract
Apoptosis. Placing death under control: D. Wallach; Nature 388, 123 (1997) Abstract
Inhibition of fas death signals by FLIPs: J. Tschopp, et al.; Curr. Opin. Immunol. 10, 552 (1998) Abstract
Inhibition of death receptor signaling by FLICE-inhibitory protein as a mechanism for immune escape of tumors: L.E. French & J. Tschopp; J. Exp. Med. 190, 891 (1999) Abstract; Full Text
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Monoclonal Antibodies
 
 
ALX-804-428 Revised 21-Oct-08
Monoclonal Antibody to FLIP (human) (NF6)
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SYNONYMS anti-CASPER (human) MAb (NF6)
anti-CLARP (human) MAb (NF6)
anti-I-FLICE (human) MAb (NF6)
anti-Usurpin (human) MAb (NF6)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FLIP
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ALX-804-428-C050   50 µg 255.00 USD Add To Cart
ALX-804-428-C100   100 µg 367.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: NF6
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human FLIP (aa 1-194).
SPECIFICITY: Recognizes short (FLIPS) and long (FLIPL) splice variants of human FLIP.
APPLICATION: Western Blot: For superior sensitivity and minimal background we recommend using the secondary PAb to Mouse IgG1 (HRP) (Prod. No. ALX-211-203).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control in Western blot see Prod. No. ALX-840-601.
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Product Specific Literature References
The role of c-FLIP in modulation of CD95-induced apoptosis: S. Scaffidi, et al.; J. Biol. Chem. 274, 1541 (1999) Abstract; Full Text
Enhancement of Apo2L/TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)-induced apoptosis in non-small cell lung cancer cell lines by chemotherapeutic agents without correlation to the expression level of cellular protease : S. Frese, et al.; J. Thorac. Cardiovasc. Surg. 123, 168 (2002) Abstract
An inducible pathway for degradation of FLIP protein sensitizes tumor cells to TRAIL-induced apoptosis: Y. Kim, et al.; J. Biol. Chem. 277, 22320 (2002) Abstract; Full Text
Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation: M. Leverkus, et al.; Mol. Cell. Biol. 23, 777 (2003) Abstract; Full Text
Suramin inhibits death receptor-induced apoptosis in vitro and fulminant apoptotic liver damage in mice: S.T. Eichhorst, et al.; Nature Med. 10, 602 (2004) Abstract
T.M. Ganten, et al.; Cell Death Differ. 11 Suppl 1, S86 (2004) Abstract
Cellular FLIP (long isoform) overexpression in T cells drives Th2 effector responses and promotes immunoregulation in experimental autoimmune encephalomyelitis: V. Tseveleki, et al.; J. Immunol. 173, 6619 (2004) Abstract; Full Text
cFLIPL inhibits tumor necrosis factor-related apoptosis-inducing ligand-mediated NF-kappaB activation at the death-inducing signaling complex in human keratinocytes: T. Wachter, et al.; J. Biol. Chem. 279, 52824 (2004) Abstract; Full Text
Elevated NF-kappaB responses and FLIP levels in leukemic but not normal lymphocytes: reduction by salicylate allows TNF-induced apoptosis: C. Rae, et al.; PNAS 104, 12790 (2007) Abstract
A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-α-induced Apoptosis: V. Oliveira, et al.; J. Biol. Chem. 283, 13174 (2008) Abstract
Stx2 Causes Apoptosis In Human Brain Microvascular Endothelial Cells Via CHOP: J. Fujii, et al.; Infect. Immun. 76, 3679 (2008) Abstract
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Monoclonal Antibodies
 
 
ALX-804-837 Revised 06-Nov-07
Monoclonal Antibody to PIDD (Anto-1)
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SYNONYMS anti-PIDD MAb (Anto-1)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Adaptor Proteins for Death Receptors Other Products
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ALX-804-837-C100   100 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Anto-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human PIDD death domain (aa 776-910).
SPECIFICITY: Recognizes human and mouse PIDD. Detects bands of ~90kDa (full length protein) and ~50kDa (cleaved protein) by Western blot.
APPLICATION: Immunohistochemistry (paraffin sections)
Western Blot (1:500)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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Product Specific Literature References
Autoproteolysis of PIDD marks the bifurcation between pro-death caspase-2 and pro-survival NF-kappaB pathway: A. Tinel, et al.; EMBO J. 26, 197 (2007) Abstract
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MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
PIDD mediates NF-kappaB activation in response to DNA damage: S. Janssens, et al.; Cell 123, 1079 (2005) Abstract
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-827 Revised 31-Oct-06
Monoclonal Antibody to RAIDD (human) (Raiddy-1)
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SYNONYMS anti-CRADD (human) MAb (Raiddy-1)
anti-Caspase and RIP Adaptor with Death Domain (human) MAb (Raiddy-1)
anti-RIP-associated Protein with a Death Domain (human) MAb (Raiddy-1)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY RAIDD / Related Products
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ALX-804-827-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Raiddy-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
QUANTITY: ≥95% (SDS-PAGE)
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human full length soluble RAIDD (aa 1-199) (Prod. No. ALX-522-085).
SPECIFICITY: Recognizes human RAIDD
APPLICATION: Immunoprecipitation (1:100)
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
General Information
RAIDD (RIP-associated ICH-1 homologous protein with a death domain) is an adaptor molecule that mediates the action of cysteine proteases involved in apoptosis. In human cell lines, the caspase-2 adaptor RAIDD interacts selectively with caspase-2 through its caspase recruitment domain (CARD) and leads to caspase-2-dependent death. RAIDD overexpression induced caspase-2, CARD- and caspase-9-dependent apoptosis of PC12 cells and sympathetic neurons. Apoptosis correlated with the formation of discrete perinuclear aggregates. Both death and aggregates required the expression of full-length RAIDD. Recently, it has been reported that activation of caspase-2 occurs in a complex that contains the death domain–containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
RAIDD aggregation facilitates apoptotic death of PC12 cells and sympathetic neurons: O. Jabado, et al.; Cell Death Differ. 11, 618 (2004) Abstract
The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress: A. Tinel and J. Tschopp; Science 304, 843 (2004) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-210-918 Revised 24-Sep-08
Polyclonal Antibody to Nod1 (human) (AL184)
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SYNONYMS anti-CARD4 (human) PAb (AL184)
anti-Caspase Recruitment Domain-containing Protein 4 (human) PAb (AL184)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Nod-complex / Related Products
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ALX-210-918-C050   50 µg 194.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Affinity-purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 2-31 (E2EQGHSEMEIIP
SESHPHIQLLKSNRELLV31) of human Nod1 [CARD4].
SPECIFICITY: Recognizes human Nod1.
APPLICATION: Western blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
NOD protein expression and function in first trimester trophoblast cells: M.J. Costello, et al.; Am. J. Reprod. Immunol. 57, 67 (2007) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Regulatory regions and critical residues of NOD2 involved in muramyl dipeptide recognition: T. Tanabe, et al.; EMBO J. 23, 1587 (2004) Abstract
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Adaptor Proteins for Death Receptors Other Products • Polyclonal Antibodies
 
 
ALX-210-920 Revised 06-Nov-07
Polyclonal Antibody to PIDD (AL233)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Adaptor Proteins for Death Receptors Other Products
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ALX-210-920-C050   50 µg 194.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human PIDD (Death Domain: aa 776-910).
SPECIFICITY: Recognizes human and the native form of mouse PIDD. Detects bands of ~90kDa (full length protein) and ~50kDa (cleaved protein) by Western blot.
APPLICATION: Immunohistochemistry (paraffin sections)
Immunoprecipitation (1:100)
Western Blot (1:500)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress: A. Tinel and J. Tschopp; Science 304, 843 (2004) Abstract
PIDD mediates NF-kB activation in Response to DNA damage: Janssens et al.; Cell 123, 1079 (2005) Abstract
Autoproteolysis of PIDD marks the bifurcation between pro-death caspase-2 and pro-survival NF-kappaB pathway: A. Tinel, et al.; EMBO J. 26, 197 (2007) Abstract
Heat shock induces apoptosis independently of any known initiator caspase-activating complex: R.S. Milleron and S.B. Bratton; J. Biol. Chem. 281, 16991 (2006) Abstract; Full Text
General Information
The death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD.  PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Pidd, a new death-domain-containing protein, is induced by p53 and promotes apoptosis: Y. Lin, et al.; Nat. Genet. 26, 122 (2000) Abstract
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Polyclonal Antibodies
 
 
ALX-210-915 Revised 25-Jan-08
Polyclonal Antibody to RAIDD (human) (AT100)
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SYNONYMS anti-CRADD (human) PAb (AT100)
anti-Caspase and RIP Adaptor with Death Domain (human) PAb (AT100)