ALEXIS Biochemicals: Monoclonal Antibody to GSK-3β (11B9)

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ALX-350-289

Revised 18-Nov-08

10Z-Hymenialdisine
SYNONYMS	4-(2-Amino-4-oxo-2-imidazolin-5-ylidene)-2-bromo-4,5,6,7-tetrahydropyrrolo[2,3-c]azepin-8-one
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - Protein Kinase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-289-C500		500 µg	190.00 USD
ALX-350-289-M001		1 mg	340.00 USD

Product Specification

FORMULA:	C₁₁H₁₀BrN₅O₂
MW:	324.1
CAS NUMBER:	82005-12-7
SOURCE/HOST:	Isolated from sponge Axinella damicornis (Stylissa damicornis).
PURITY:	≥97%
APPEARANCE:	Yellow needles.
SOLUBILITY:	Soluble in DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.
HAZARD:	TOXIC.
IDENTITY:	Identity determined by ¹H- NMR, ¹³C-NMR and MS.

Product Description

Originally isolated from the sponges Axinella verrucosa and Acantella aurantiaca. Potent inhibitor of mitogen-activated protein kinase kinase-1 (MEK-1) (IC₅₀=6nM). Blocks the in vivo phosphorylation of the microtubule-binding protein tau at sites that are hyperphosphorylated by glycogen synthase kinase-β (GSK-3β) and CDK5/p35 in Alzheimer’s disease. Inhibitor of DNA damage checkpoint at G2 phase (IC₅₀=6µM), cyclin-dependent kinases CDK1/cyclin B (IC₅₀=22nM), CDK2/cyclin A (IC₅₀=70nM), CDK2/cyclin E (IC₅₀=40nM), CDK4/cyclin D1 (IC₅₀=600nM), CDK5/p25 (IC₅₀=28nM), GSK-3β (IC₅₀=10nM), and casein kinase 1 (CK1) (IC₅₀=35nM). Inhibitor of NF-κB activation and of various pro-inflammatory cytokines such as IL-1, IL-2 (IC₅₀=2.4µM), IL-6, IL-8, TNF-α (IC₅₀=1.4µM) and nitric oxide (NO) (IC₅₀=0.8µM) in a variety of cell lines.

Product Specific Literature References

Isolation and x-ray crystal structure of a novel bromo-compound from two marine sponges: G. Cimino, et al.; Tetrahedron Lett. 23, 767 (1982)
Bioactive alkaloids from the tropical marine sponge Axinella carteri: A. Supriyono, et al.; Z. Naturforsch. [C] 50, 669 (1995) Abstract
The natural product hymenialdisine inhibits interleukin-8 production in U937 cells by inhibition of nuclear factor-kappaB: J.J. Breton & M.C. Chabot-Fletcher; J. Pharmacol. Exp. Ther. 282, 459 (1997) Abstract; Full Text
Inhibition of NFkappaB-mediated interleukin-1beta-stimulated prostaglandin E2 formation by the marine natural product hymenialdisine: A. Roshak, et al.; J. Pharmacol. Exp. Ther. 283, 955 (1997) Abstract; Full Text
Inhibition of interleukin-1-induced proteoglycan degradation and nitric oxide production in bovine articular cartilage/chondrocyte cultures by the natural product, hymenialdisine: A.M. Badger, et al.; J. Pharmacol. Exp. Ther. 290, 587 (1999) Abstract; Full Text
Inhibition of cyclin-dependent kinases, GSK-3beta and CK1 by hymenialdisine, a marine sponge constituent: L. Meijer, et al.; Chem. Biol. 7, 51 (2000) Abstract
Inhibition of the G2 DNA damage checkpoint and of protein kinases Chk1 and Chk2 by the marine sponge alkaloid debromohymenialdisine: D. Curman, et al.; J. Biol. Chem. 276, 17914 (2001) Abstract; Full Text
An investigation of cell proliferation and soluble mediators induced by interleukin 1beta in human synovial fibroblasts: comparative response in osteoarthritis and rheumatoid arthritis: H. Inoue, et al.; Inflamm. Res. 50, 65 (2001) Abstract
Aldisine alkaloids from the Philippine sponge Stylissa massa are potent inhibitors of mitogen-activated protein kinase kinase-1 (MEK-1): D. Tasdemir, et al.; J. Med. Chem. 45, 529 (2002) Abstract
Inhibition of cytokine production by hymenialdisine derivatives: V. Sharma, et al.; J. Med. Chem. 47, 3700 (2004) Abstract

Further Categories Containing This Product:

CDK & Cyclin Inhibitors • MAPK Pathway Inhibitors • Natural Products - NF-kB Pathway Inhibitors • NF-kB Pathway Inhibitors • Natural Products for Cell Cycle Research • Natural Products - Nitric Oxide Pathway Modulators • DNA Damage Checkpoint Other Products • GSK-3 Inhibitors • Alkaloids • Marine Natural Products

ALX-350-294

Revised 16-Jun-08

Manzamine A
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - Anti-inflammatory Agents

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-294-M001		1 mg	195.00 USD

Product Specification

FORMULA:	C₃₆H₄₄N₄O
MW:	548.8
CAS NUMBER:	104196-68-1
SOURCE/HOST:	Isolated from Xestospongia sp.
PURITY:	>98% (HPLC)
FORMULATION:	Yellow oil
SOLUBILITY:	Soluble in DMSO, methanol or 100% ethanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

β-Carboline alkaloid with anti-inflammatory, antimalarial, antifungal, anti-HIV-1and insecticidal activity. Also active against the Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus. Inhibits GSK-3. Has native fluorescence, highest when solubilized in methanol (Ex: 340nm, Em: 387nm).

Product Specific Literature References

Four new bioactive manzamine-type alkaloids from the Philippine marine sponge Xestospongia ashmorica: R.A. Edrada, et al.; J. Nat. Prod. 59, 1056 (1996) Abstract
In vivo antimalarial activity of the beta-carboline alkaloid manzamine A: K.K. Ang, et al.; Antimicrob. Agents Chemother. 44, 1645 (2000) Abstract; Full Text
Immune-mediated parasite clearance in mice infected with Plasmodium berghei following treatment with manzamine A: K.K. Ang, et al.; Parasitol. Res. 87, 715 (2001) Abstract
New manzamine alkaloids with activity against infectious and tropical parasitic diseases from an Indonesian sponge: K.V. Rao, et al.; J. Nat. Prod. 66, 823 (2003) Abstract
Spectrofluorimetric determination of manzamine A in spiked human urine and plasma: A. Khalil, et al.; Pharmazie 58, 385 (2003) Abstract
New manzamine alkaloids from an Indo-Pacific sponge. Pharmacokinetics, oral availability, and the significant activity of several manzamines against HIV-I, AIDS opportunistic infections, and inflammatory diseases: M. Yousaf, et al.; J. Med. Chem. 47, 3512 (2004) Abstract
Antimalarial activity of a new family of analogues of manzamine A: J.D. Winkler, et al.; Org. Lett. 8, 2591 (2006) Abstract
Glycogen synthase kinase-3 (GSK-3) inhibitory activity and structure-activity relationship (SAR) studies of the manzamine alkaloids. Potential for Alzheimer’s disease: M. Hamann, et al.; J. Nat. Prod. 70, 1397 (2007) Abstract

Further Categories Containing This Product:

Natural Products - Antimalarial Agents • Malaria / Related Products • Natural Products with Antibiotic Activity • Natural Products - Antiviral / anti-HIV Agents • Natural Products - Antifungal Agents • GSK-3 Inhibitors • Pesticides / Related Products • Alkaloids

ALX-350-371

Revised 10-Mar-08

Harmine
SYNONYMS	Leucoharmine Banisterine Telepathine Yageine 7-Methoxy-1-methyl-9H-pyrido[3,4-b]indole
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Natural Products - DNA Regulation / Transcription

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-371-M250		250 mg	22.00 USD

Product Specification

FORMULA:	C₁₃H₁₂N₂O
MW:	212.3
CAS NUMBER:	442-51-3
MERCK INDEX:	14: 4616
RTECS:	UV0175000
SOURCE/HOST:	Originally isolated from Peganum harmala.
PURITY:	≥98%
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide or 100% ethanol; slightly soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HAZARD:	HARMFUL.

Product Description

Alkaloid of the β-carboline family. Regulates PPARγ expression. Inhibits the Wnt signalling pathway in a cell-specific manner. Down-regulates inflammatory gene expression (TNF-α, IL-1β, iNOS). Reversibly inhibits monoamine oxidase inhibitor A (MAO-A), but has no effect on MAO-B.

Product Specific Literature References

The chemotherapy of derivatives of harmine and harmaline. I: C.E. Coulthard, et al.; Biochee. J. 27, 727 (1933) Abstract; Full Text
The chemotherapy of derivatives of harmine and harmaline. II: C.E. Coulthard; Biochem. J. 28, 264 (1934) Abstract; Full Text
Interrelationships between amphetamine and harmala alkaloids: H. Schmitt; Nature 203, 877 (1964) Abstract
Effect of moclobemide on rat brain monoamine oxidase A and B: comparison with harmaline and clorgyline: J. Gerardy; Prog. Neuropsychopharmacol. Biol. Psychiatry 18, 793 (1994) Abstract
Hypothermic effect of harmala alkaloid in rats: involvement of serotonergic mechanism: A.F. Abdel-Fattah, et al.; Pharmacol. Biochem. Behav. 52, 421 (1995) Abstract
The mode of action of thiazolidinediones: H. Hauner; Diabet. Metab. Res. Rev. 18, S10 (2002) Abstract
Antitumor and neurotoxic effects of novel harmine derivatives and structure-activity relationship analysis: Q. Chen, et al.; Int. J. Cancer 114, 675 (2005) Abstract
The small molecule harmine is an antidiabetic cell-type-specific regulator of PPARgamma expression: H. Waki, et al.; Cell Metab. 5, 357 (2007) Abstract

Further Categories Containing This Product:

Natural Products - Other Signal Transduction Pathway Modulators • Interleukin-1 [IL-1] & Receptors / Related Products • TNF-alpha & TNF Receptors Other Products • Wnt Signalling Pathway Other Products • NOS Regulation • Antidiabetic Agents / Related Products • Alkaloids

ALX-430-156

Revised 21-Apr-08

6BIO
SYNONYMS	(2’Z,3’E)-6-Bromoindirubin-3’-oxime
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	GSK-3 Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-430-156-M001		1 mg	40.00 USD

Product Specification

FORMULA:	C₁₆H₁₀BrN₃O₂
MW:	356.2
CAS NUMBER:	667463-62-9
PURITY:	≥98%
APPEARANCE:	Dark red solid.
SOLUBILITY:	Soluble in DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.
HAZARD:	IRRITANT.

Product Description

Inhibitor of phosphoinositide-dependent kinase 1 (PDK1). Potent, reversible and ATP-competitive inhibitor of glycogen synthase kinase-3α/β (GSK-3α/β).

Product Specific Literature References

Structural basis for the synthesis of indirubins as potent and selective inhibitors of glycogen synthase kinase-3 and cyclin-dependent kinases: P. Polychronopoulos, et al.; J. Med. Chem. 47, 935 (2004) Abstract
7-Bromoindirubin-3’-oxime induces caspase-independent cell death: J. Ribas, et al.; Oncogene 25, 6304 (2006) Abstract
Inverse in silico screening for identification of kinase inhibitor targets: S. Zahler, et al.; Chem. Biol. 14, 1207 (2007) Abstract
An integrated computational approach to the phenomenon of potent and selective inhibition of aurora kinases B and C by a series of 7-substituted indirubins: V. Myrianthopoulos, et al.; J. Med. Chem. 50, 4027 (2007) Abstract

ALX-270-271	Indirubin-3'-monoxime
ALX-430-149	7BIO
ALX-430-157	Me-7BIO
ALX-270-296	Indirubin-5-sulfonic acid . sodium salt
ALX-270-361	Indirubin
ALX-270-424	5-Iodo-indirubin-3'-monoxime

SPECIES CROSSREACTIVITY:	Human
CLONE:	5E1
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.2mg/ml
PURITY:	Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
PURITY DETAIL:	Protein A-affinity purified.
IMMUNOGEN:	Recombinant human melusin (integrin β1-binding protein 2).
SPECIFICITY:	Recognizes human melusin. Cross-reacts weakly with mouse melusin.
APPLICATION:	ELISA Immunocytochemistry (5µg/ml) Immunohistochemistry (5µg/ml) Immunoprecipitation Western Blot (2µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

SPECIES CROSSREACTIVITY:	Human Mouse Dog
CLONE:	9G2
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN:	Recombinant human β-catenin.
SPECIFICITY:	Recognizes exon 3 (α-catenin-binding site) of human, mouse, rat (weak) and dog β-catenin.
APPLICATION:	Immunocytochemistry (0.1-1µg/ml) Immunoprecipitation Western Blot (0.1µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED:	Yes (Prod. No. ALX-840-027)

ALX-804-060	Monoclonal Antibody to β-Catenin (NT/Exon 2) (7D11)
ALX-804-259	Monoclonal Antibody to β-Catenin (dephospho, aa 35-50) (7A7)

SPECIES CROSSREACTIVITY:	Human Mouse Dog
CLONE:	7D8
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:	Recombinant human β-catenin.
SPECIFICITY:	Recognizes the C-terminus (exon 14) of human, mouse, rat (weak) and a dog β-catenin.
APPLICATION:	Immunocytochemistry (0.1-1µg/ml) Immunohistochemistry (paraffin sections) Immunoprecipitation Western blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride. Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:	Included (Prod. No. ALX-840-027).

ALX-804-259	Monoclonal Antibody to β-Catenin (dephospho, aa 35-50) (7A7)
ALX-804-058	Monoclonal Antibody to β-Catenin (Exon 3) (9G2)

SPECIES CROSSREACTIVITY:	Human Mouse Rat Dog
CLONE:	15D9
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:	Recombinant human α-catenin.
SPECIFICITY:	Recognizes human, mouse, rat and dog α-catenin. Does not cross-react with β-catenins.
APPLICATION:	Immunocytochemistry (0.1-1µg/ml; formaldehyde fixation is tolerated) Immunoprecipitation Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:	Yes (Prod. No. ALX-840-026).