ALEXIS Biochemicals: Polyclonal Antibody to p56dok-2 (human)

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ALX-380-116

Revised 07-Apr-08

Hypothemycin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antibiotics - Antifungal

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-116-C250		250 µg	140.00 USD
ALX-380-116-M001		1 mg	420.00 USD

Product Specification

FORMULA:	C₁₉H₂₂O₈
MW:	378.4
CAS NUMBER:	76958-67-3
SOURCE/HOST:	Isolated from Phoma sp.
PURITY:	≥98% (HPLC)
APPEARANCE:	Off-white solid.
SOLUBILITY:	Soluble in DMSO or acetone; insoluble in methanol or water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Exhibits antifungal and cytotoxic activity against some tumor cell lines partly attributed to inhibition of Ras-inducible genes. Inhibits proliferation of mouse and human T cells and modulates production of cytokines during T cell activation. Facilitates the ubiquitinylation process of cyclin D1. Has been identified as a potent and selective inhibitor of threonine/tyrosine-specific kinase, MEK, and other protein kinases that contain a conserved cysteine residue in the ATP-binding site in both in vitro and in vivo studies.

Product Specific Literature References

Metabolites of pyrenomycetes XIII: Structure of (+) hypothemycin, an antibiotic macrolide from hypomyces trichothecoides: M.S.R. Nair & S.T. Carey; Tetrahedron Lett. 21, 2011 (1980)
Metabolites of pyrenomycetes. XIV: Structure and partial stereochemistry of the antibiotic macrolides hypothemycin and dihydrohypothemycin: M.S.R. Nair, et al.; Tetrahedron 37, 2445 (1981)
Revised structure and stereochemistry of hypothemycin: T. Agatsuma et al.; Chem. Pharm. Bull. 41, 373 (1993)
Antitumor efficacy of hypothemycin, a new Ras-signaling inhibitor: H. Tanaka, et al.; Jpn. J. Cancer Res. 90, 1139 (1999) Abstract
Hypothemycin inhibits the proliferative response and modulates the production of cytokines during T cell activation: R. Camacho, et al.; Immunopharmacology 44, 255 (1999) Abstract
Suppression of oncogenic transformation by hypothemycin associated with accelerated cyclin D1 degradation through ubiquitin-proteasome pathway: H. Sonoda, et al.; Life Sci. 65, 381 (1999) Abstract
Resorcylic acid lactones: naturally occurring potent and selective inhibitors of MEK: A. Zhao, et al.; J. Antibiot. 52, 1086 (1999) Abstract
Targeted covalent inactivation of protein kinases by resorcylic acid lactone polyketides: A. Schirmer, et al.; PNAS 103, 4234 (2006) Abstract; Full Text
Chemistry and biology of resorcylic acid lactones: N. Winssinger, et al.; Chem. Commun. (Camb). 1, 22 (2007), (Review) Abstract

Further Categories Containing This Product:

MAPK Pathway Inhibitors • Antitumor Antibiotics • Antibiotics - Protein Kinase Inhibitors • Tyrosine Kinase Inhibitors • Antitumor Agents (DNA Interaction & Gene Regulation) • Antibiotics - Proteasome / Ubiquitin Modulators • Ubiquitination Other Products • CDKs & Cyclins Other Products • Ras Signalling Pathway Other Products

ALX-210-746

Revised 11-Apr-06

Polyclonal Antibody to p56dok-2 (human)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Ras Signalling Pathway Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-746-C200		200 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From rabbit.
CONCENTRATION:	2mg/ml.
PURITY DETAIL:	Epitope-affinity purified IgG.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to C-terminal aa 393-412(A³⁹³SGWQPGTEYDNVVLKKGPK⁴¹²) of human p56^dok-2.
SPECIFICITY:	Recognizes human p56^dok-2. Detects a band of ~60kDa by Western blot.
APPLICATION:	Immunocytochemistry (1:300) Western Blot (1:1'000)
QUALITY CONTROL:	Western blot on human U937 cells or human T cell line Jurkat.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	After opening, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-165-033.

General Information

p56^dok-2, a 56kDa tyrosine phosphorylated protein, purified and cloned from p210^bcr-abl expressing cells, is able to bind to p120^RasGAP. It was shown to be 35% identical to p62^dok-1.

General Literature References

Molecular cloning and characterization of p56dok-2 defines a new family of RasGAP-binding proteins: A. Di Cristofano, et al.; J. Biol. Chem. 273, 4827 (1998) Abstract