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Ceramides / Cerebrosides / Sphingolipids / Related Products
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ALX-303-001 Revised 03-Feb-05
Ceramide I (bovine brain)
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SYNONYMS N-Acylsphingosine (bovine brain)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Ceramides / Cerebrosides / Sphingolipids / Related Products
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ALX-303-001-M025   25 mg 65.00 USD Add To Cart
Product Specification
SOURCE/HOST: Semisynthetic (from bovine brain cerebrosides).
PURITY: ≥98%
APPEARANCE: White powder.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Contains saturated, unsaturated and α-hydroxy fatty acids. Modulator of cell growth and differentiation.
Product Specific Literature References
Role of ceramide as a lipid mediator of 1 alpha,25-dihydroxyvitamin D3- induced HL-60 cell differentiation: T. Okazaki, et al.; J. Biol. Chem. 265, 15823 (1990) Abstract; Full Text
 
 
ALX-303-002 Revised 24-Feb-05
Ceramide II (bovine brain)
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SYNONYMS Ceramide HFA (bovine brain)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Ceramides / Cerebrosides / Sphingolipids / Related Products
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ALX-303-002-M025   25 mg 65.00 USD Add To Cart
Product Specification
SOURCE/HOST: Semisynthetic (from bovine brain cerebrosides).
PURITY: ≥98%
APPEARANCE: White powder.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Contains α-hydroxy fatty acids. Prepared from cerebrosides by a modified protocol of H.E. Carter et al.; J. Lipid Res. 2, 228 (1961)
 
 
ALX-306-007 Revised 05-Feb-08
D-erythro-Sphingosine, N-Stearoyl-
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SYNONYMS N-Stearoyl ceramide
C18-Ceramide
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Ceramides / Cerebrosides / Sphingolipids / Related Products
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ALX-306-007-M001   1 mg 20.00 USD Add To Cart
ALX-306-007-M005   5 mg 60.00 USD Add To Cart
Product Specification
FORMULA: C36H71NO3
MW: 565.9
CAS NUMBER: 2304-81-6
SOURCE/HOST: Semisynthetic.
PURITY: ≥98%
APPEARANCE: White to off-white powder.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

 
 
ALX-270-341 Revised 28-Sep-07
Epoxyquinone G109 (racemic)
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PRODUCT LINE Neurobiology
PRODUCT CATEGORY Ceramides / Cerebrosides / Sphingolipids / Related Products
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ALX-270-341-M002   2 mg 190.00 USD Add To Cart
Product Specification
FORMULA: C18H27NO4
MW: 321.4
CAS NUMBER: 163972-12-1 ((+)-enantiomer)
PURITY: ≥98%
APPEARANCE: Pale yellow powder.
SOLUBILITY: Soluble in DMSO or methanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
HAZARD: TOXIC.

Product Description
Potent and selective irreversible inhibitor of neutral sphingomyelinase (N-SMase).
Product Specific Literature References
Manumycin A and its analogues are irreversible inhibitors of neutral sphingomyelinase: C. Arenz, et al.; ChemBioChem 2, 141 (2001) Abstract
Endothelial apoptosis induced by inhibition of integrins αvβ3 and αvβ5 involves ceramide metabolic pathways: A. Erdreich-Epstein, et al.; Blood 105, 4353 (2005) Abstract
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Other Toxins
 
 
ALX-306-030 Revised 06-Dec-06
Globotrihexosylceramide
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SYNONYMS Gb3
PRODUCT LINE Immunology
PRODUCT CATEGORY Natural Killer T [NKT] Cells / Related Products
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ALX-306-030-C100   100 µg 85.00 USD Add To Cart
ALX-306-030-M001   1 mg 455.00 USD Add To Cart
Product Specification
FORMULA: C62H117NO18
MW: 1164.6
PURITY: ≥95% (1H-NMR)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in pyridine or chloroform:methanol (2:1).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C

Product Description
Negative control for isoglobotrihexosylceramide (iGb3) (Prod. No. ALX-306-028). Does not stimulate natural killer T (NKT) cells.
Product Specific Literature References
Chemoenzymatic Syntheses of iGb3 and Gb3: Q. Yao et al.; Org. Lett. 8, 911 (2006) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Solubilization:
Our recommendation is to dissolve product in chloroform: abs. methanol 2:1. Aliquot into glass tubes, dry and store at 4°C. Resuspended in abs. methanol (at concentrations not higher than 1%) prior to cell assays.
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Ceramides / Cerebrosides / Sphingolipids / Related Products
 
 
ALX-306-028 Revised 05-May-08
Isoglobotrihexosylceramide
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SYNONYMS iGb3
PRODUCT LINE Immunology
PRODUCT CATEGORY Natural Killer T [NKT] Cells / Related Products
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ALX-306-028-C100   100 µg 85.00 USD Add To Cart
ALX-306-028-M001   1 mg 455.00 USD Add To Cart
Product Specification
FORMULA: C62H117NO18
MW: 1164.6
PURITY: ≥95% (1H-NMR)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in pyridine or chloroform:methanol (2:1).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C

Product Description
Lysosomal glycosphingolipid proposed to be the natural ligand of NKT cells. Stimulates natural killer T (NKT) cells similar to α-GalCer. For negative control compound see globotrihexosylceramide (Gb3) (Prod. No. ALX-306-030).
Product Specific Literature References
[1] Lysosomal glycosphingolipid recognition by NKT cells: D. Zhou, et al.; Science 306, 1786 (2004) Abstract
[2] Synthesis and biological evaluation of alpha-galactosylceramide (KRN7000) and isoglobotrihexosylceramide (iGb3): C. Xia, et al.; Bioorg. Med. Chem. Lett. 16, 2195 (2006) Abstract
[3] Chemoenzymatic Syntheses of iGb3 and Gb3: Q. Yao et al.; Org. Lett. 8, 911 (2006) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Solubilization:
Our recommendation is to dissolve product in chloroform: abs. methanol 2:1. Aliquot into glass tubes, dry and store at 4°C. Resuspended in abs. methanol (at concentrations not higher than 1%) prior to cell assays. Note: The use of DMSO may abolish the iGb3 activity and should be avoided [2].

For expanding human NKT cells from freshly isolated peripheral blood mononuclear cells (PBMC):
Mix iGb3 at 100ng/ml with PBMC and culture for 4-5 days.

For the stimulation of mouse NKT cells:
The bone marrow derived dendritic cells (BMDC) is the most efficient cell type that present iGb3. Use 50’000-250’000 of BMDCs pulsed overnight with 100ng/ml of iGb3 and 50’000 freshly purified NKT cells as a responder. Use tetramer sorting to purifiy the NKT cells from spleen and before mixing with BMDCs, rest them in culture medium for 12 hours and then wash with medium to avoid a high background. MACS sorting technique is not recommended. Alternatively, NKT cell hybridomas can be used to detect the ligand. (For reference see: http://home.uchicago.edu/~dzhou/Testing%20iGb3%20ligand.htm)
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Ceramides / Cerebrosides / Sphingolipids / Related Products
 
 
ALX-306-020 Revised 22-Sep-08
Lignoceric ceramide
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SYNONYMS Tetracosenamide
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Ceramides / Cerebrosides / Sphingolipids / Related Products
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ALX-306-020-M005   5 mg 85.00 USD Add To Cart
Product Specification
FORMULA: C42H83NO3
MW: 650.1
CAS NUMBER: 34435-05-7
SOURCE/HOST: Synthetic.
PURITY: ≥98%
APPEARANCE: White to off-white crystalline solid.
SOLUBILITY: 20mg/ml soluble in 100% ethanol, DMSO or dimethyl formamide; also soluble in aqueous buffers.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C. We do not recommend storing aqueous solutions for more than one day.

Product Description
Naturally occurring ceramide and an integral part of the sphingomyelin cycle [1]. C24-N-acyl sphingosines are the most abundant ceramide species in human plasma [2] and in rabbit leukocytes [3], where they make up to 50-60% of total ceramides. Lignoceric ceramide stimulates the proliferation of basophilic erythroblasts, suggesting a possible role in erythrocyte maturation [3].
Product Specific Literature References
[1] Ceramide signalling and the immune response: L.R. Ballou, et al.; Biochim. Biophys. Acta 1301, 273 (1996), (Review) Abstract
[2] Identification and quantitative determination of ceramides in human plasma: K. Samuelsson; Scand. J. Clin. Lab. Invest. 27, 371 (1971) Abstract
[3] Stimulation of erythroblast maturation in vitro by sphingolipids: R.B. Clayton, et al.; J. Lipid Res. 15, 557 (1974) Abstract
 
 
ALX-306-031 Revised 23-Sep-08 New product
α-Mannosyl Ceramide
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SYNONYMS α-ManCer
PRODUCT LINE Immunology
PRODUCT CATEGORY Natural Killer T [NKT] Cells / Related Products
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ALX-306-031-C250   250 µg 120.00 USD Add To Cart
ALX-306-031-M001   1 mg 250.00 USD Add To Cart
Product Specification
FORMULA: C50H99NO9
MW: 858.3
PURITY: ≥95%
APPEARANCE: White to brown powder.
SOLUBILITY: Soluble in pyridine or warm DMSO (4mg/ml).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After reconstitution, aliquot into glass vials.

Product Description
Potent stimulator of natural killer T (NKT) cells expressing the invariant Vα19-Jα26 T cell receptor α chain (Vα19 NKT cells).
Product Specific Literature References
Synthetic alpha-mannosyl ceramide as a potent stimulant for an NKT cell repertoire bearing the invariant Valpha19-Jalpha26 TCR alpha chain: N. Okamoto, et al.; Chem. Biol. 12, 677 (2005) Abstract
Induction of promotive rather than suppressive immune responses from a novel NKT cell repertoire Valpha19 NKT cell with alpha-mannosyl ceramide analogues consisting of the immunosuppressant ISP-I as the sphingosine unit: M. Shimamura, et al.; Eur. J. Med. Chem. 41, 569 (2006) Abstract
Modulation of Valpha19 NKT cell immune responses by alpha-mannosyl ceramide derivatives consisting of a series of modified sphingosines: M. Shimamura, et al.; Eur. J. Immunol. 37, 1836 (2007) Abstract
General Information
Natural killer T cells (NKT cells) are important regulators of the immune system. They share properties of both innate as well as adaptive immunity [1]. They express T cell receptors (TCR) on their surface consisting of an invariable α-chain and a somewhat variable β-chain [2]. The majority of NKT cells express the invariable α-chain Vα14-Jα18 (Vα14) in mouse or its homolog in humans Vα24-Jα18 (Vα24) [3]. Vα14 NKT cells recognize α-GalCer presented by CD1d [4, 5].
Recently, NKT cells expressing the invariant Vα19-Jα26 TCR α-chain (Vα19 NKT cells) have been found in peripheral blood lymphocytes and in the liver [6]. They recognize α-ManCer presented by MR1 (Major histocompatibility complex, class I-related) and respond with production of large amounts of immunoregulatory cytokines [7, 8].
General Literature References
[1] Mouse CD1-specific NK1 T cells: development, specificity, and function: A. Bendelac, et al.; Annu. Rev. Immunol. 15, 535 (1997) Abstract
[2] Germline-encoded recognition of diverse glycolipids by natural killer T cells: J.P. Scott-Browne, et al.; Nat. Immunol. 8, 1105 (2007) Abstract
[3] CD1d-restricted mouse V alpha 14 and human V alpha 24 T cells: lymphocytes of innate immunity: K. Benlagha & A. Bendelac; Semin. Immunol. 12, 537 (2000) Abstract
[4] CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides: T. Kawano, et al.; Science 278, 1626 (1997) Abstract
[5] CD1d-lipid-antigen recognition by the semi-invariant NKT T-cell receptor: N.A. Borg, et al.; Nature 448, 44 (2007) Abstract
[6] Presence of a novel subset of NKT cells bearing an invariant V(alpha)19.1-J(alpha)26 TCR alpha chain: M. Shimamura & Y.Y. Huang; FEBS Lett. 516, 97 (2002) Abstract
[7] Synthetic alpha-mannosyl ceramide as a potent stimulant for an NKT cell repertoire bearing the invariant Valpha19-Jalpha26 TCR alpha chain: N. Okamoto, et al.; Chem. Biol. 12, 677 (2005) Abstract
[8] Modulation of Valpha19 NKT cell immune responses by alpha-mannosyl ceramide derivatives consisting of a series of modified sphingosines: M. Shimamura, et al.; Eur. J. Immunol. 37, 1836 (2007) Abstract
Further Categories Containing This Product:
Ceramides / Cerebrosides / Sphingolipids / Related Products
 
 
ALX-350-241 Revised 07-Dec-07
Manumycin A
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics - Other Signal Transduction Pathway Modulators
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ALX-350-241-M001   1 mg 58.00 USD Add To Cart
ALX-350-241-M005   5 mg 170.00 USD Add To Cart
ALX-350-241-M010   10 mg 260.00 USD Add To Cart
Product Specification
FORMULA: C31H38N2O7
MW: 550.7
CAS NUMBER: 52665-74-4
SOURCE/HOST: Isolated from Streptomyces parvulus.
PURITY: ≥96% (HPLC)
APPEARANCE: Yellow to brown powder.
SOLUBILITY: Soluble in methanol or DMSO; insoluble in water.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
IDENTITY: Determined by 1H-NMR.

Product Description
Potent, selective and competitive inhibitor of Ras farnesyltransferase. Does not affect geranylgeranyltransferase. Inhibition is competitive with respect to farnesyl pyrophosphate and non-competitive with respect to Ras. Inhibits neutral sphingomyelinase.
Product Specific Literature References
Inhibitors of Ras farnesyltransferases: F. Tamanoi; TIBS 18, 349 (1993), (Review) Abstract
Farnesyltransferase inhibitors: Ras research yields a potential cancer therapeutic: J.B. Gibbs, et al.; Cell 77, 175 (1994) Abstract
Manumycin inhibits ras signal transduction pathway and induces apoptosis in COLO320-DM human colon tumour cells: A. Di Paolo, et al.; Br. J. Cancer 82, 905 (2000) Abstract
Manumycin A and its analogues are irreversible inhibitors of neutral sphingomyelinase: C. Arenz, et al.; ChemBioChem 2, 141 (2001) Abstract
Enhancement of manumycin A-induced apoptosis by methoxyamine in myeloid leukemia cells: M. She, et al.; Leukemia 19, 595 (2005) Abstract
Binding of manumycin A inhibits IkappaB kinase beta activity: M. Bernier, et al.; J. Biol. Chem. 281, 2551 (2006) Abstract
Further Categories Containing This Product:
Farnesylation / Isoprenylation Reagents • Ceramides / Cerebrosides / Sphingolipids / Related Products • Antibiotics - Apoptosis Inducers & Inhibitors
 
 
ALX-804-196 Revised 27-Aug-08
Monoclonal Antibody to Ceramide (MID 15B4)
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PRODUCT LINE Neurobiology
PRODUCT CATEGORY Ceramides / Cerebrosides / Sphingolipids / Related Products
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ALX-804-196-T050   50 tests 315.00 USD Add To Cart
Product Specification
CLONE: MID 15B4
ISOTYPE: Mouse IgM
SOURCE/HOST: Purified from ascites by gel filtration on sephacryl S-300.
QUANTITY: 1ml (50 tests). Suggested amount: 20µl/test.
CONCENTRATION: 200µg/ml
PURITY: ≥95%
FORMULATION: Liquid. In PBS, pH 7.2, containing 0.5M sodium chloride, 0.1% BSA and 0.09% sodium azide.
IMMUNOGEN: Ceramide (sphingosine-[trans-D-erythro-2-amino-4-octadecene-1,3-diol]) conjugated to BSA.
SPECIFICITY: Recognizes C16- and C24-ceramide, dihydroceramide, sphingomyelin and phosphatidylcholine in highly artificial lipid overlay test systems. Under more physiological in vitro and in vivo conditions highly specific for ceramide and does not cross-react with sphingomyelin, cholesterol or other phospholipids (see lit. ref. [1], [2], [3]).
APPLICATION: ELISA (1:10)
Flow Cytometry (see [2])
Immunocytochemistry (see [11,12])
Immunohistochemistry (1:10)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
[1] Structural determinants of sphingolipid recognition by commercially available anti-ceramide antibodies: L.A. Cowart, et al.; J. Lipid Res. 43, 2042 (2002) Abstract; Full Text
[2] CD95 Signaling Via Ceramide-rich Membrane Rafts: H. Grassme, et al.; J. Biol. Chem. 276, 20589 (2002) Abstract; Full Text
[3] Ceramide-Rich Membrane Rafts Mediate CD40 Clustering: H. Grassme, et al.; J. Immunol. 168, 298 (2002) Abstract; Full Text
[4] Ceramide inhibits the potassium channel Kv1.3 by the formation of membrane platforms: J. Bock, et al.; BBRC 305, 890 (2003) Abstract
[5] Host defense against Pseudomonas aeruginosa requires ceramide-rich membrane rafts: H. Grassme, et al.; Nat. Med. 9, 322 (2003) Abstract
[6] Regulation of cell death in mitotic neural proge