• Home
  • Sitemap
  • Help
  • Technical Support
  • Contact
Adaptor Proteins & Interaction Domains / Related Products
You are here: Product Lines > Signal Transduction > Adaptor Proteins & Interaction Domains / Related Products
 
Toolbar - View Selection
 
 Items 100-104 of 104 Page 6 of 6 Select Page: << 1 2 3 4 5 6   
ALX-522-085 Revised 21-Dec-06
RAIDD (human) (recombinant)
Add to Clipboard
SYNONYMS CRADD (human) (recombinant)
Caspase and RIP Adaptor with Death Domain (human) (recombinant)
RIP-associated Protein with a Death Domain (human) (recombinant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY RAIDD / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-085-C050   50 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~48kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. Full length human RAIDD (aa 1-199) is fused to a N-terminal tag.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
APPLICATION: Use as positive control for the PAb to RAIDD (human) (AT100) (Prod. No. ALX-210-915).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
RAIDD (RIP-associated ICH-1 homologous protein with a death domain) is an adaptor molecule that mediates the action of cysteine proteases involved in apoptosis. In human cell lines, the caspase-2 adaptor RAIDD interacts selectively with caspase-2 through its caspase recruitment domain (CARD) and leads to caspase-2-dependent death. RAIDD overexpression induced caspase-2, CARD- and caspase-9-dependent apoptosis of PC12 cells and sympathetic neurons. Apoptosis correlated with the formation of discrete perinuclear aggregates. Both death and aggregates required the expression of full-length RAIDD. Recently, it has been reported that activation of caspase-2 occurs in a complex that contains the death domain–containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress: A. Tinel and J. Tschopp; Science 304, 843 (2004) Abstract
RAIDD aggregation facilitates apoptotic death of PC12 cells and sympathetic neurons: O. Jabado, et al.; Cell Death Differ. 11, 618 (2004) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
APO-54N-038 Revised 10-Oct-08 New product
RIG-I (human) (IntraCellular) Detection Set [For ELISA Application]
Add to Clipboard
SYNONYMS Retinoic Acid-inducible Gene 1 (human) (IntraCellular) Detection Set [For ELISA Application]
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
APO-54N-038-KI01   1 Set 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 0.4ng/ml (range 0 to 32ng/ml).
QUANTITY: For 5x96 wells.
KIT/SET CONTAINS: 1 vial Standard (lyophilized) (STD).
1 vial Coating Antibody (COAT).
1 vial Detection Antibody (DET).
APPLICATION: For the quantitative determination of human RIG-I in cell extracts.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
The innate immune response to viruses is initiated upon detection of viral RNA generated during the life cycle of most viruses [1]. Recognition of the viral RNA is mediated by either the two cytoplasmic RNA helicases Retinoic acid Inducible Gene-1 (RIG-I ; Ddx58) and Melanoma Differentiation Associated gene 5 (MDA5 / Helicard) [2] or by Toll-like Receptor 3 (TLR3) located to an endosomal-like compartment [3]. RIG-I and MDA5 belong to the family of RIG-I like receptor (RLR) together with the protein LGP2 whose function is still elusive [4, 5]. Upon binding to RNA, RIG-I or MDA5 binds a new mediator named Cardif, MAVS, IPS-1 or VISA [6, 7] that signals through transcription factors, such as IRF-3, IRF-7, and NF-κB leading to the activation of interferons (IFNs) and other inflammatory cytokine genes. RIG-I is activated by dsRNA and 5′-ppp RNA. RIG-I has been shown to trigger innate immune signaling pathways during infection by orthomyxoviruses, rhabdovirus, vesicular stomatitis virus, and paramyxoviruses; and possibly by the filovirus Ebola virus and by the Epstein-Barr virus [8]. Virus infection induces a rapid production of IFN-α/β that leads to expression of hundreds of interferon-stimulated genes (ISGs) whose products direct antiviral actions. RIG-I belongs to the ISG gene family and monitoring its levels using the RIG-I (human) Detection Set (IC) can be used as indicator of IFN production and virus infection.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Recognition of viruses by innate immunity: O. Takeuchi & S. Akira; Immunol. Rev. 220, 214 (2007) Abstract
[2] MDA5/RIG-I and virus recognition: O. Takeuchi & S. Akira; Curr. Opin. Immunol. 20, 17 (2008) Abstract
[3] TLR3: Interferon induction by double-stranded RNA including poly(I:C): M. Matsumoto & T. Seya; Adv. Drug Deliv. Rev. 60, 805 (2008) Abstract
[4] Cytoplasmic recognition of RNA: M. Yoneyama, et al.; Adv. Drug Deliv. Rev. 60, 841 (2008) Abstract
[5] Loss of DExD/H box RNA helicase LGP2 manifests disparate antiviral responses: T. Venkataraman, et al.; J. Immunol. 178, 6444 (2007) Abstract; Full Text
[6] Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
[7] IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
[8] Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008) Abstract; Full Text
Related Products
Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling / Related Products • ELISA Sets • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products
 
 
ALX-840-604 Revised 01-Mar-07
Positive Control (Cell Lysate) for Antibodies to RIP2 (human)
Add to Clipboard
SYNONYMS Positive Control (Cell Lysate) for Antibodies to Receptor-interacting Protein 2 (human)
Positive Control (Cell Lysate) for Antibodies to CARDIAK (human)
Positive Control (Cell Lysate) for Antibodies to CARD-containing IL-1β Converting Enzyme Associated Kinase (human)
Positive Control (Cell Lysate) for Antibodies to RICK (human)
Positive Control (Cell Lysate) for Antibodies to RIPK2 (human)
Positive Control (Cell Lysate) for Antibodies to Receptor-interacting Serine-Threonine Kinase 2 (human)
PRODUCT LINE Immunology
PRODUCT CATEGORY Positive Controls (Cell Lysates & Extracts)
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-840-604-R100   100 µl 70.00 USD Add To Cart
Product Specification
SOURCE/HOST: Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human RIP2 (receptor-interacting protein 2) (aa 1-541) fused at the C-terminus to a FLAG®-tag.
QUANTITY: 100µl corresponding to 2x106 cells.
FORMULATION: Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION: Positive control in Western blot for MAb to RIP2 (human) (Nick-1) (Prod. No. ALX-804-139). Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Nod-complex / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products
 
 
ALX-168-003 Revised 02-Apr-03
Tyrosine Phosphorylation Site Inhibitor
Add to Clipboard
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Tyrosine Kinase Inhibitors
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-168-003-M001   1 mg 60.00 USD Add To Cart
ALX-168-003-M005   5 mg 240.00 USD Add To Cart
Product Specification
SEQUENCE: H-Gly-Ser-Phe-Leu-Val-Arg-Glu-Ser-OH
FORMULA: C39H63N11O13
MW: 894.0
PURITY: ≥97%
APPEARANCE: White lyophilized powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Inhibitor of EGF receptor binding to SH2. The peptide corresponds to a highly conserved amino acid sequence common in a variety of SH2-containing proteins. It is the minimum essential sequence for recognition of the phosphotyrosine site.
Product Specific Literature References
Highly conserved eight amino acid sequence in SH2 is important for recognition of phosphotyrosine site: M. Hidaka, et al.; BBRC 180, 1490 (1991) Abstract
Further Categories Containing This Product:
Peptides • SH2 & SH3 Domains
 
 
© 2008 ALEXIS Corporation, Privacy Policy, Disclaimer, Feedbackheader picture © micronaut.ch, another CS2 website

Catalogs / Brochures

E-Newsletter

 

Shopping Cart [0]

 

Distributors

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.