ALEXIS Biochemicals: Polyclonal Antibody to DPR1

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ALX-350-371

Revised 10-Mar-08

Harmine
SYNONYMS	Leucoharmine Banisterine Telepathine Yageine 7-Methoxy-1-methyl-9H-pyrido[3,4-b]indole
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Natural Products - DNA Regulation / Transcription

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-371-M250		250 mg	22.00 USD

Product Specification

FORMULA:	C₁₃H₁₂N₂O
MW:	212.3
CAS NUMBER:	442-51-3
MERCK INDEX:	14: 4616
RTECS:	UV0175000
SOURCE/HOST:	Originally isolated from Peganum harmala.
PURITY:	≥98%
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide or 100% ethanol; slightly soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HAZARD:	HARMFUL.

Product Description

Alkaloid of the β-carboline family. Regulates PPARγ expression. Inhibits the Wnt signalling pathway in a cell-specific manner. Down-regulates inflammatory gene expression (TNF-α, IL-1β, iNOS). Reversibly inhibits monoamine oxidase inhibitor A (MAO-A), but has no effect on MAO-B.

Product Specific Literature References

The chemotherapy of derivatives of harmine and harmaline. I: C.E. Coulthard, et al.; Biochee. J. 27, 727 (1933) Abstract; Full Text
The chemotherapy of derivatives of harmine and harmaline. II: C.E. Coulthard; Biochem. J. 28, 264 (1934) Abstract; Full Text
Interrelationships between amphetamine and harmala alkaloids: H. Schmitt; Nature 203, 877 (1964) Abstract
Effect of moclobemide on rat brain monoamine oxidase A and B: comparison with harmaline and clorgyline: J. Gerardy; Prog. Neuropsychopharmacol. Biol. Psychiatry 18, 793 (1994) Abstract
Hypothermic effect of harmala alkaloid in rats: involvement of serotonergic mechanism: A.F. Abdel-Fattah, et al.; Pharmacol. Biochem. Behav. 52, 421 (1995) Abstract
The mode of action of thiazolidinediones: H. Hauner; Diabet. Metab. Res. Rev. 18, S10 (2002) Abstract
Antitumor and neurotoxic effects of novel harmine derivatives and structure-activity relationship analysis: Q. Chen, et al.; Int. J. Cancer 114, 675 (2005) Abstract
The small molecule harmine is an antidiabetic cell-type-specific regulator of PPARgamma expression: H. Waki, et al.; Cell Metab. 5, 357 (2007) Abstract

Further Categories Containing This Product:

Natural Products - Other Signal Transduction Pathway Modulators • Interleukin-1 [IL-1] & Receptors / Related Products • TNF-alpha & TNF Receptors Other Products • Wnt Signalling Pathway Other Products • NOS Regulation • Antidiabetic Agents / Related Products • Alkaloids

ALX-804-262

Revised 13-Jun-08

Monoclonal Antibody to LEF (2D12)
SYNONYMS	anti-Lymphoid Enhancer Factor MAb (2D12)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Transcription Factors Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-262-C100		100 µg	453.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	2D12
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:	Recombinant LEF (lymphoid enhancer factor) (aa 1-85).
SPECIFICITY:	Recognizes human and mouse (weak) LEF. Detects a band of ~68kDa by Western blot. Does not cross-react with TCF3 (T cell factor 3) or TCF4 (T cell factor 4).
APPLICATION:	Immunocytochemistry (use at 1µg/ml in PBS) Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.1µg/ml for HRPO/ECL detection).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.

General Information

LEF is a transcription factor of the LEF/TCF protein family. LEF interacts with cytoplasmic β-catenin and regulates the expression of genes containing a LEF/TCF binding site. The formation of LEF-β-catenin heterodimers is an important step in the wnt/wg signal transduction pathway.

Further Categories Containing This Product:

Wnt Signalling Pathway Other Products • Monoclonal Antibodies

ALX-215-059

Revised 25-Jun-08

Polyclonal Antibody to DPR1
SYNONYMS	anti-Dapper Homolog 1 PAb anti-HNG3 PAb anti-Hepatocellular Carcinoma Novel Gene 3 Protein PAb anti-Thymus-expressed Novel Gene 3 Protein PAb
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Wnt Signalling Pathway Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-215-059-C100		100 µg	290.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	From rabbit.
CONCENTRATION:	0.75mg/ml
PURITY DETAIL:	Protein A-affinity purified.
FORMULATION:	Liquid. In PBS
IMMUNOGEN:	Recombinant human DPR1 (Dapper homolog 1) (aa 282-516).
SPECIFICITY:	Recognizes human and mouse DPR1. Detects a band of ~90kDa.
APPLICATION:	Immunocytochemistry (1:100-1:200) Immunoprecipitation (1:100-1:200) Western Blot (1:1’000-1:5’000) Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least one year after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Specific Literature References

Dapper 1 Antagonizes Wnt Signaling by Promoting Dishevelled Degradation: L. Zhang, et al.; J. Biol. Chem. 281, 8607 (2006) Abstract; Full Text

General Information

Members of the Dact protein family initially were identified through binding to Dishevelled (Dvl), a cytoplasmic protein central to Wnt signalling. DPR1 inhibits Wnt signalling. During mouse development, DPR1 is detected in the presomitic mesoderm and somites during segmentation, in the limb bud mesenchyme and other mesoderm-derived tissues, and in the central nervous system (CNS). Dact paralogs have distinct signalling and developmental roles and suggest they may differentially contribute to postnatal brain physiology.

General Literature References

HDPR1, a novel inhibitor of the WNT/β-catenin signaling, is frequently
downregulated in hepatocellular carcinoma: involvement of methylationmediated
gene silencing: TO. Yau, et al.; Oncogene 24, 1607 (2005) Abstract
Dapper, a Dishevelled-Associated Antagonist of β-Catenin and JNK Signaling, Is Required for Notochord Formation: B. Cheyette, et al.; Developmental Cell 2, 449 (2002) Abstract

Further Categories Containing This Product:

Polyclonal Antibodies

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