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ALX-804-018
Revised 11-Jan-05
Monoclonal Antibody to Calmodulin (2D1)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calmodulin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-018-R200
200 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Rat
Others
CLONE:
2D1
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified calmodulin from
Dictyostelium discoideum
strain AX3.
SPECIFICITY:
Recognizes rat, bovine, chicken brain, chlamydomonas and dictyostelium calmodulin. In competition assays reacts with the SDS denatured, but not native form of bovine brain calmodulin. Detects a band of ~17kDa by Western blot. Does not cross-react with parvalbumin, troponin, S-100 and myosin light chain kinase.
APPLICATION:
ELISA
Immunocytochemistry (1:100)
Western Blot (1:500)
Note:
Addition of EGTA to buffers completely inhibits antibody staining.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Production and specificity of monoclonal antibodies against calmodulin from Dictyostelium discoideum:
D. Hulen, et al.; Cell Motility & Cytoskeleton
18
, 113 (1991)
Abstract
Calmodulin and the contractile vacuole complex in mitotic cells of Dictyostelium discoideum
:
Q. Zhu, et al.; J. Cell Sci.
104
, 1119 (1993)
Abstract
;
Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-017
Revised 03-Mar-05
Monoclonal Antibody to Calmodulin (6D4)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calmodulin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-017-R200
200 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Rat
Others
CLONE:
6D4
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified calmodulin from
Dictyostelium discoideum
strain.
SPECIFICITY:
Recognizes rat, bovine, chicken, chlamydomonas and dictyostelium calmodulin. Detects a band of ~17kDa by Western blot. Does not cross-react with parvalbumin, troponin, S-100 and myosin light chain kinase (MLCK).
APPLICATION:
ELISA
Immunocytochemistry (1:100)
Western Blot (1:2'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Production and specificity of monoclonal antibodies against calmodulin from Dictyostelium discoideum:
D. Hulen, et al.; Cell Motility & Cytoskeleton
18
, 113 (1991)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Addition of EGTA to buffers completely inhibits antibody staining. Immunohistochemical staining of calmodulin in dictyostelium cells with Prod. No.
ALX-804-017
results in staining of the contractile vacuoles.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-053
Revised 11-Jan-05
Monoclonal Antibody to Calpain (156)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calpains / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-053-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE:
156
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified 28kDa calpain subunit from bovine skeletal muscle.
SPECIFICITY:
Recognizes human and bovine 28kDa calpain subunit. Detects a band of ~28kDa by Western blot. Does not cross-react with the human 80kDa m-calpain subunit.
APPLICATION:
Western Blot (1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Description
The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (µ-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.
µ- and m-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.
Product Specific Literature References
Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains:
J. Cong, et al.; J. Biol. Chem.
268
, 25740 (1993)
Abstract
;
Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-051
Revised 09-Oct-08
Monoclonal Antibody to µ-Calpain (Domain II) (2H2A7C2)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calpains / Related Products
Ordering Information
Product Numbers:
Format:
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Quantity:
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ALX-804-051-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
Pig
Others
CLONE:
2H2A7C2
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites containing 0.05% sodium azide.
IMMUNOGEN:
Purified bovine µ-calpain.
SPECIFICITY:
Recognizes an epitope in domain II (aa 245-265) of µ-calpain from several mammalian sources (e.g. human, rat, pig, bovine). Detects aband of ~80kDa by Western blot. Does not cross-react withm-calpain, n-calpain, calmodulin or calpastatin.
APPLICATION:
Immunocytochemistry (1:25)
Western Blot (1:1'000)
Note:
Not recommended for immunoprecipitation.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
General Information
The calpain (Ca
2+
-dependent proteinase or Ca
2+
-activated neutral protease) system consists of two ubiquitous forms of calpain (m-calpain and µ-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered Ca
2+
homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.
m- and µ-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of Ca
2+
, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.
BACKGROUND/TECHNICAL INFORMATION
Immunocytochemical staining of µ-calpain in pig LLC-PK1 cells with Prod. No. ALX-804-051 results in diffuse cytoplasmic staining.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-050
Revised 10-Nov-08
Monoclonal Antibody to µ-Calpain (Domain III) (9A4H8D3)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calpains / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-050-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Pig
Others
CLONE:
9A4H8D3
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites containing 0.05% sodium azide.
IMMUNOGEN:
Purified bovine µ-calpain.
SPECIFICITY:
Recognizes an epitope in domain III (aa 465-520) of µ-calpain from several mammalian sources (e.g. human, mouse, rat, pig, bovine, hamster, rabbit). Detects a band of ~80kDa by Western blot. Does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
APPLICATION:
Immunocytochemistry (1:100)
Immunohistochemistry (frozen sections)
Western Blot (1:2’000)
Note:
Not recommended for immunoprecipitation.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Description
This antibody has been successfully used in Western blot procedures to detect µ-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, and rat myoblasts, kidney, liver and spleen. Immunofluorescence staining of µ-calpain in porcine LLC-PK1 cells with 804-050 results in diffuse cytoplasmic staining.
Product Specific Literature References
A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells:
R.D. Lane, et al.; Exp. Cell Res.
203
, 5 (1992)
Abstract
Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains:
J. Cong, et al.; J. Biol. Chem.
268
, 25740 (1993)
Abstract
;
Full Text
Calpain-dependent proteolytic cleavage of the p35 cyclin-dependent kinase 5 activator to p25:
G. Kusakawa, et al.; J. Biol. Chem.
275
, 17166 (2000)
Abstract
;
Full Text
Calpain is a major cell death effector in selective striatal degeneration induced in vivo by 3-nitropropionate: implications for Huntington’s disease:
N. Bizat, et al.; J. Neurosci.
23
, 5020 (2003)
Abstract
;
Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-055
Revised 11-Aug-08
Monoclonal Antibody to Calpastatin (Domain II) (2G11D6)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calpains / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-055-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
Dog
Pig
Others
CLONE:
2G11D6
ISOTYPE:
Mouse IgG2a
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified bovine skeletal muscle calpastatin.
SPECIFICITY:
Recognizes an epitope in domain II (aa 295-501) of human, rat, dog, pig, bovine and rabbit calpastatin. Detects bands of ~150, ~125, ~90 and ~70kDa by Western blot, which have been suggested to correspond to protein isoforms translated from different start sites. Does not cross-react with calpains or calmodulin.
APPLICATION:
Immunocytochemistry (1:1’000)
Immunohistochemistry (frozen sections)
Western Blot (1:5’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-054
Revised 04-Mar-08
Monoclonal Antibody to Calpastatin (Domain IV) (1F7E3D10)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calpains / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-054-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
Pig
Others
CLONE:
1F7E3D10
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites containing 0.05% sodium azide.
IMMUNOGEN:
Purified bovine skeletal muscle 80kDa subunit of m-calpastatin.
SPECIFICITY:
Recognizes an epitope in domain IV (aa 543-673) of human, rat, pig and bovine calpastatin. Detects bands of ~150, ~125, ~90 and ~70kDa by Western blot, which have been suggested to correspond to protein isoforms translated from different start sites. Does not cross-react with calpains or calmodulin.
APPLICATION:
Immunocytochemistry (1:200)
Western Blot (1:5'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-089
Revised 27-Mar-08
Monoclonal Antibody to Calsequestrin (VIIID12)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calcium Signalling (Intracellular) Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-089-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Dog
Others
CLONE:
VIIID12
ISOTYPE:
Mouse IgG2b
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified rabbit skeletal muscle sarcoplasmic reticulum.
SPECIFICITY:
Recognizes mouse, rat, rabbit and dog type I (slow) and type II (fast) calsequestrin. Detects a band of ~63kDa by Western blot.
APPLICATION:
Immunocytochemistry
Western Blot (1:1’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-090
Revised 30-Jan-06
Monoclonal Antibody to Dihydropyrine Receptor α1 (1A)
SYNONYMS
anti-DHP Receptor α1 MAb (1A)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Ca2+ Channels (L-type)
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-090-R400
400 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE:
1A
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Contains 0.05% sodium azide.
IMMUNOGEN:
Purified rabbit muscle T-tubule DHP receptor (dihydropyridine receptor).
SPECIFICITY:
Recognizes the α1 subunit of human, mouse, rat, guinea pig and rabbit DHP receptor. Detects a band of ~200kDa by Western blot.
APPLICATION:
Immunohistochemistry (frozen sections (1:200))
Immunoprecipitation
Western Blot (1:500)
Functional Application (inhibits DHP-sensitive calcium current in certain cell lines)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-091
Revised 28-Feb-08
Monoclonal Antibody to Dihydropyrine Receptor α2 (20A)
SYNONYMS
anti-DHP Receptor α2 MAb (20A)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Ca2+ Channels (L-type)
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-091-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE:
20A
ISOTYPE:
Mouse IgG2a
FORMULATION:
Liquid. Diluted ascites containing 0.05% sodium azide.
IMMUNOGEN:
Purified rabbit DHP receptor (dihydropyridine receptor).
SPECIFICITY:
Recognizes the α2 subunit of human, mouse, rat, guinea pig and rabbit DHP receptor. Detects a band of ~220kDa (non-reducing conditions) or ~143kDa (reducing conditions) by Western blot.
APPLICATION:
Immunocytochemistry
: 1:250
Immunohistochemistry
(paraffin or frozen): 1:500. Staining of DHP in rabbit skeletal muscle results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines
Western Blot
: 1:500
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-092
Revised 22-May-08
Monoclonal Antibody to Na+/Ca2+ Exchanger (C2C12)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calcium Signalling (Intracellular) Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-092-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
Dog
Others
CLONE:
C2C12
ISOTYPE:
Mouse IgM
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified dog cardiac Na
+
/ Ca
2+
exchanger.
SPECIFICITY:
Recognizes human, rat, dog, rabbit and guinea pig Na
+
/ Ca
2+
exchanger. The epitope for this antibody is on the intracellular side of the plasma membrane (aa 371-525). Detects a band of ~120kDa (mature exchanger), as well as bands of ~70kDa (proteolytic fragment) and ~160kDa (non-reduced exchanger) by Western blot.
APPLICATION:
Immunocytochemistry (1:200)
Immunohistochemistry
Immunoprecipitation
Western Blot (1:1’000; not recommended for rat tissue)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Description
The Na
+
/Ca
2+
exchanger of cardiac sarcolemma rapidly transports Ca
2+
during excitation-concentration coupling and is the dominant myocardial Ca
2+
efflux mechanism.
Product Specific Literature References
Distribution of the Na+/Ca2+ exchange protein in mammalian cardiac myocytes: An immunofluorescence and immunocolloidal gold-labelling study:
J.S. Frank, et al.; J. Cell. Biol.
117
, 337 (1992)
Abstract
Mapping of the cardiac sodium-calcium exchanger with monoclonal antibodies:
H. Porzig, et al.; Am. J. Physiol.
265
, C748 (1993)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-093
Revised 21-May-08
Monoclonal Antibody to Phospholamban (2D12)
SYNONYMS
anti-PLB (2D12) MAb
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calcium Signalling (Intracellular) Other Products
