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Signal Transduction
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ALX-204-005 Revised 07-Aug-07
Calmodulin (wheat)
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SYNONYMS CaM (wheat)
Phosphodiesterase 3':5'-cyclic Nucleotide Activator (wheat)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calmodulins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-204-005-C250   250 µg 70.00 USD Add To Cart
ALX-204-005-M001   1 mg 190.00 USD Add To Cart
ALX-204-005B-C050 Biotin 50 µg 290.00 USD Add To Cart
ALX-204-005L-C050 Fluorescein 50 µg 290.00 USD Add To Cart
ALX-204-005RH-C050 Rhodamine 50 µg 290.00 USD Add To Cart
Product Specification
MW: ~17kDa (SDS-PAGE)
MERCK INDEX: 14: 1719
SOURCE/HOST: Isolated from wheat germ (Triticum aestivum).
PURITY: ≥98% (SDS-PAGE)
FORMULATION: Lyophilized from 5% sucrose, pH 7.0, containing 10mM sodium phosphate.
RECONSTITUTION: Reconstitute with 1ml distilled water.
SPECIFIC ACTIVITY: ≥35’000U/mg protein. One unit will stimulate 0.01 unit of 3’:5’-cyclic nucleotide phosphodiesterase in a 3ml reaction volume at pH 7.5 and 30°C, to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100µM Ca2+.
APPLICATION: Unconjugated CaM: Can be independently derivatized at both N- and C-terminal regions within the molecule for structural, proteomic biomarker discovery and protein-protein interaction studies.

CaM-Biotin, -Fluorescein & -Rhodamine: Protein array applications for the detection of calmodulin binding proteins (CBPs), Western blot and others.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light. Avoid freeze/thaw cycles.
Product Description

Wheat germ calmodulin sequence is unique in that it contains cysteine (Cys26) and only a single tyrosine (Tyr138), in contrast to mammalian CaMs, which have two tyrosine residues and lack cysteine. This allows wheat germ CaM to be independently derivatized at both N- and C-terminal regions. Therefore it is ideal for applications requiring both calmodulin activation and residue specific tagging. Wheat germ CaM also has no tryptophan resulting in a distinctive absorbance spectrum when compared to mammalian CaM sequences. The conjugated formats are labelled at Cys26.
Product Specific Literature References
Site-specific derivatives of wheat germ calmodulin. Interactions with troponin and sarcoplasmic reticulum: G.M. Strasburg, et al.; J. Biol. Chem. 263, 542 (1988) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P04464: Calmodulin (wheat)
General Literature References
A rapid and sensitive method for detection and quantification of calcineurin and calmodulin-binding proteins using biotinylated calmodulin: M.L. Billingsley, et al.; PNAS 82, 7585 (1985) Abstract; Full Text
Further Categories Containing This Product:
Natural Proteins
 
 
ALX-212-014 Revised 30-Jan-07
Calmodulin (wheat & pig) (Immobilized High Loading)
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SYNONYMS CaM (wheat & pig) (Immobilized High Loading)
Phosphodiesterase 3’:5’-cyclic Nucleotide Activator (wheat & pig) (Immobilized High Loading)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calmodulins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-212-014-R200   200 µl 290.00 USD Add To Cart
Product Specification
MERCK INDEX: 14: 1719
SOURCE/HOST: Isolated from wheat germ (Triticum aestivum) and pig brain. Immobilized via SH group (Cys26) (wheat) and amino group (pig) to epoxy activated resin (non-agarose).
CONCENTRATION: Calmodulin content: 1ml of resin bound with 5-5.5mg of wheat germ calmodulin.
FORMULATION: 50% slurry in 3M NaCl, 20mM sodium phosphate buffer, pH 7.4 and 1mM EGTA.
APPLICATION: Useful for pull down experiments and proteomic applications requiring a high capacity precipitation reagent. Dense resin can be easily separated from extracts by centrifugation using batch or spin column procedures. Resin can be reused but proteolysis from crude samples will reduce binding capacity on repeat cycles.

Loading conditions: 100µM Ca2+, with protease inhibitors and appropriate buffers.
Elution conditions: Buffers with 1mM EDTA or EGTA.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description

Wheat germ calmodulin sequence is unique in that it contains cysteine (Cys26) and only a single tyrosine (Tyr138), in contrast to mammalian CaMs, which have two tyrosine residues and lack cysteine. Immobilized via SH group (Cys26) (wheat) and amino group (pig) to epoxy activated resin (non-agarose). Thus allowing multiple orientations of bound calmodulin for efficient capture of calmodulin binding proteins.
Product Specific Literature References
Site-specific derivatives of wheat germ calmodulin. Interactions with troponin and sarcoplasmic reticulum: G.M. Strasburg, et al.; J. Biol. Chem. 263, 542 (1988) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P04464: Calmodulin (wheat)
Further Categories Containing This Product:
Natural Proteins
 
 
ALX-212-013 Revised 30-Jan-07
Calmodulin (wheat) (Immobilized High Loading)
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SYNONYMS CaM (wheat) (Immobilized High Loading)
Phosphodiesterase 3’:5’-cyclic Nucleotide Activator (wheat) (Immobilized High Loading)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calmodulins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-212-013-R200   200 µl 290.00 USD Add To Cart
Product Specification
MERCK INDEX: 14: 1719
SOURCE/HOST: Isolated from wheat germ (Triticum aestivum). Coupled primarily through SH group (Cys26) to epoxy activated resin (non-agarose).
CONCENTRATION: Calmodulin content: 1ml of resin bound with 5-5.5mg of wheat germ calmodulin.
FORMULATION: 50% slurry in 3M NaCl, 20mM sodium phosphate buffer, pH 7.4 and 1mM EGTA.
APPLICATION: Useful for pull down experiments and proteomic applications requiring a high capacity precipitation reagent. Dense resin can be easily separated from extracts by centrifugation using batch or spin column procedures. Resin can be reused but proteolysis from crude samples will reduce binding capacity on repeat cycles.

Loading conditions: 100µM Ca2+, with protease inhibitors and appropriate buffers.
Elution conditions: Buffers with 1mM EDTA or EGTA.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description

Wheat germ calmodulin sequence is unique in that it contains cysteine (Cys26) and only a single tyrosine (Tyr138), in contrast to mammalian CaMs, which have two tyrosine residues and lack cysteine. This allows wheat germ CaM to be independently derivatized at both N- and C-terminal regions. Coupled to epoxy activated resin primarily through SH group at Cys26.
Product Specific Literature References
Site-specific derivatives of wheat germ calmodulin. Interactions with troponin and sarcoplasmic reticulum: G.M. Strasburg, et al.; J. Biol. Chem. 263, 542 (1988) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P04464: Calmodulin (wheat)
Further Categories Containing This Product:
Natural Proteins
 
 
ALX-260-037 Revised 30-May-07
Calpain Inhibitor I
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SYNONYMS Ac-LL-Norleucinal
Ac-Leu-Leu-Nle-CHO
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calpains / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-260-037-M010   10 mg 80.00 USD Add To Cart
ALX-260-037-M050   50 mg 310.00 USD Add To Cart
Product Specification
FORMULA: C20H37N3O4
MW: 383.5
CAS NUMBER: 110044-82-1
SOURCE/HOST: Synthetic.
PURITY: ≥96% (HPLC)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO, 100% ethanol or methanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Protect from light and moisture.
Product Description
Inhibits calpain I (Ki=190nM), calpain II (Ki=220nM), cathepsin B (Ki=150nM) and cathepsin L (Ki=0.5nM). Inhibits neutral cysteine proteases and the proteasome. Protects against neuronal damage caused by hypoxia and ischemia. Inhibits apoptosis in thymocytes and metamyelocytes. Inhibits cell cycle progression at the G1/S border and metaphase/anaphase in CHO cells by inhibiting cyclin B degradation. Also prevents nitric oxide production by activated macrophages by interfering with the transcription of inducible nitric oxide synthase (iNOS; NOS II). Inhibits the proteolysis of IκB-α and IκB-β by the ubiquitin-proteasome complex.
Product Specific Literature References
Serine and cysteine proteinase inhibitors prevent nitric oxide production by activated macrophages by interfering with transcription of the inducible NO synthase gene: J.M. Griscavage, et al.; BBRC 215, 721 (1995) Abstract
Further Categories Containing This Product:
Proteasome / Related Products • Cathepsin Inhibitors • Proteases Other Products
 
 
ALX-260-038 Revised 15-Nov-05
Calpain Inhibitor II
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SYNONYMS Ac-LLM-CHO
Ac-Leu-Leu-Met-CHO
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calpains / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-260-038-M010   10 mg 75.00 USD Add To Cart
ALX-260-038-M050   50 mg 290.00 USD Add To Cart
Product Specification
FORMULA: C19H35N3O4S
MW: 401.6
CAS NUMBER: 110115-07-6
PURITY: ≥96%
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in 100% ethanol, methanol or DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Stock solutions are stable for up to 3 months when stored at -20°C.
HANDLING: Protect from light and moisture.
Product Description
Inhibitor of calpain I, calpain II, cathepsin B and cathepsin L.
Further Categories Containing This Product:
Cathepsin Inhibitors
 
 
ALX-260-014 Revised 19-Aug-08
Calpeptin
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SYNONYMS Z-Leu-norleucinal
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calpains / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-260-014-M005   5 mg 85.00 USD Add To Cart
ALX-260-014-M010   10 mg 160.00 USD Add To Cart
Product Specification
FORMULA: C20H30N2O4
MW: 362.5
CAS NUMBER: 117591-20-5
PURITY: ≥94% (HPLC)
APPEARANCE: White to off-white powder.
SOLUBILITY: 5mg/ml soluble in DMSO or dimethyl formamide.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Stock solutions are stable for up to 2 months when stored at -20°C.
HANDLING: Packaged under inert gas. After reconstitution, prepare aliquots and store at -20°C. Protect from light and moisture.

Product Description
Membrane permeable inhibitor of calpain I and II and cathepsin L. Promotes neurite elongation in differentiating PC12 cells. Prevents Ca2+-ionophore induced degradation of actin binding protein and P235 in platelets. Inhibits myosin light chain phosphorylation in platelets stimulated by collagen, ionomycin (Prod. No. ALX-450-006) or thrombin.
Product Specific Literature References
Inhibitory effect of di- and tripeptidyl aldehydes on calpains and cathepsins: T. Sasaki, et al.; J. Enz. Inhib. 3, 195 (1990) Abstract
Cell-penetrating inhibitors of calpain: S. Mehdi; TIBS 16, 150 (1991) Abstract
Specificity of calcium-activated neutral proteinase (CANP) inhibitors for human mu CANP and mCANP: K. Saito and R.A. Nixon; Neurochem. Res. 18, 231 (1993) Abstract
Calpeptin, a calpain inhibitor, promotes neurite elongation in differentiating PC12 cells: M. Pinter, et al.; Neurosci. Lett. 170, 91 (1994) Abstract
Further Categories Containing This Product:
Cathepsin Inhibitors
 
 
ALX-350-027 Revised 19-Aug-08
Calphostin C
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SYNONYMS UCN-1028C
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Protein Kinase Inhibitors
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-027-C100   100 µg 85.00 USD Add To Cart
ALX-350-027-M001   1 mg 595.00 USD Add To Cart
Product Specification
FORMULA: C44H38O14
MW: 790.8
CAS NUMBER: 121263-19-2
SOURCE/HOST: Isolated from Cladosporium cladosporioides MST-FP1798.
PURITY: ≥95% (HPLC)
APPEARANCE: Dark red solid.
SOLUBILITY: Soluble in 100% ethanol, methanol, DMSO or dimethyl formamide; insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Stock solutions are stable for 3 months when stored at -20°C.
HANDLING: Protect from light.

Product Description
Potent and selective inhibitor of the protein kinase C (PKC) regulatory site of diacylglycerol and phorbol esters. At higher concentrations inhibits myosin light chain kinase, cAMP-dependent protein kinase, protein kinase G and pp60v-src protein tyrosine kinase. Induces apoptotic DNA fragmentation and cell death. Kills breast cancer cells. Has antiviral potential. Inhibits cardiac L-type Ca2+ channels.
Product Specific Literature References
Calphostin C (UCN-1028C), a novel microbial compound, is a highly potent and specific inhibitor of protein kinase C: E. Kobayashi, et al.; BBRC 159, 548 (1989) Abstract
Potent and specific inhibitors of protein kinase C of microbial origin: T. Tamaoki and H. Nakano; Biotechnology 8, 732 (1990) Abstract
Inhibition of protein kinase C by calphostin C is light-dependent: R.F. Bruns, et al.; BBRC 176, 288 (1991) Abstract
Irreversible oxidative inactivation of protein kinase C by photosensitive inhibitor calphostin C: R. Gopalakrishna, et al.; FEBS Lett. 314, 149 (1992) Abstract
Calphostin C, a specific protein kinase C inhibitor, activates human neutrophils: effect on phospholipase A2 and aggregation: S. Svetlov and S. Nigam; Biochim. Biophys. Acta 1177, 75 (1993) Abstract
Induction of apoptotic DNA fragmentation and cell death in HL-60 human promyelocytic leukemia cells by pharmacological inhibitors of protein kinase C: W.D. Jarvis, et al.; Cancer Res. 54, 1707 (1994) Abstract
Growth inhibition of herpes simplex virus-type 1 in calphostin C-treated astrocytes: C.G. Castagnino, et al.; Intervirology 38, 332 (1995) Abstract
Calphostin C, a widely used protein kinase C inhibitor, directly and potently blocks L-type Ca channels: H.C. Hartzell & A. Rinderknecht; Am. J. Physiol. 270, C1293 (1996) Abstract
Pharmacokinetic features and metabolism of calphostin C, a naturally occurring perylenequinone with antileukemic activity: C.L. Chen, et al.; Pharm. Res. 16, 1003 (1999) Abstract
Potent killing of paclitaxel- and doxorubicin-resistant breast cancer cells by calphostin C accompanied by cytoplasmic vacuolization: B. Guo, et al.; Breast Cancer Res. Treat. 82, 125 (2003) Abstract
Further Categories Containing This Product:
Myosin Light Chain Kinase Inhibitors • Tyrosine Kinase Inhibitors • PKA Inhibitors • PKC Inhibitors • PKG Inhibitors • Ca2+ Channels (L-type) • Natural Products - Antitumor Reagents • Natural Products - Antiviral / anti-HIV Agents • Natural Products - Apoptosis Inducers & Inhibitors
 
 
ALX-201-269 Revised 18-Mar-08
Calreticulin (rabbit) (recombinant)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calreticulin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-269-C025   25 µg 170.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 1.2mg/ml
PURITY: ≥99%
FORMULATION: Liquid. In 100mM HEPES, pH 7.5, containing 150mM NaCl and 5mM CaCl2.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Delineation of the lectin site of the molecular chaperone calreticulin: S.P. Thomson and D.B. Williams; Cell Stress Chaperones 10, 242 (2005) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P15253: Calreticulin (rabbit)
Further Categories Containing This Product:
ER-Golgi Intermediate Compartment [ERGIC] / Related Products • Recombinant Proteins / Fusion Proteins • Endoplasmatic Reticulum Stress
 
 
ALX-350-014 Revised 03-Apr-08
Calyculin A
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Other Signal Transduction Pathway Modulators
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-014-C010   10 µg 65.00 USD Add To Cart
ALX-350-014-C025   25 µg 110.00 USD Add To Cart
ALX-350-014-C050   50 µg 190.00 USD Add To Cart
ALX-350-014-C100   100 µg 320.00 USD Add To Cart
Product Specification
FORMULA: C50H81N4O15P
MW: 1009.2
CAS NUMBER: 101932-71-2
SOURCE/HOST: Isolated from Discodermia calyx.
PURITY: ≥95%
APPEARANCE: Lyophilized.
SOLUBILITY: Soluble in DMSO, 100% ethanol, methanol, acetonitrile, chloroform, methylene chloride or benzene. Insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: May by subject to isomerization when in solution. Biological activity is not affected by isomerization.
HANDLING: Protect from light and moisture.
HAZARD: TOXIC. MAY BE CARCINOGENIC.

Product Description
Potent cell permeable inhibitor with high specificity for protein phosphatase 1 (PP1) and 2A (PP2A). Inhibits p130cas tyrosine phosphorylation. Enhances the phosphorylation level of NF-κB. Affects intracellular signalling processes that require 14-3-3. Potent non-phorbol type tumor promoter. Prevents γ-radiation induced apoptosis.
Product Specific Literature References
Okadaic acid: an additional non-phorbol-12-tetradecanoate-13-acetate- type tumor promoter: M. Suganuma, et al.; PNAS 85, 1768 (1988) Abstract
Protein phosphatases come of age: P.Cohen & P.T. Cohen; J. Biol. Chem. 264, 21435 (1989) Abstract; Full Text
Calyculin A and okadaic acid: inhibitors of protein phosphatase activity: H. Ishihara, et al.; BBRC 159, 871 (1989) Abstract
The structure and regulation of protein phosphatases: P. Cohen; Ann. Rev. Biochem. 58, 453 (1989) Abstract
Calyculin A, an inhibitor of protein phosphatases, a potent tumor promoter on CD-1 mouse skin: M. Suganuma, et al.; Cancer Res. 50, 3521 (1990) Abstract
Nonphorbol tumor promoters okadaic acid and calyculin-A induce membrane translocation of protein kinase C: R. Gopalakrishna; BBRC 189, 950 (1992) Abstract
Comparative effects of protein phosphatase inhibitors (okadaic