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Delta-like [DLL] & Jagged / Related Products
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ALX-201-382 Revised 24-Jul-08
DLL1 (human) (recombinant)
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SYNONYMS Delta-like Protein 1 (human) (recombinant)
Delta1 (human) (recombinant)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Delta-like [DLL] & Jagged / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-382-C010   10 µg 295.00 USD Add To Cart
ALX-201-382-C050   50 µg 795.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. Signal peptide and extracellular domain of recombinant human DLL1 (Delta-like protein 1; Delta1) (aa 1-545) is fused at the C-terminus to a FLAG®-tag.
CONCENTRATION:

PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
Notch signalling pathways play key roles in cell-fate determination and differentiation in many tissues during embryonic and postnatal development. Notch ligands are divided into two subclasses, the delta and the serrate family [1]. Delta-like 1, 3, and 4 (DLL1, -3, -4), JAG1 and JAG2 with DSL (Delta, Serrate, Lag2) domain are typical Notch ligands, while DNER, F3/Contactin and NB-3 without DSL domain are atypical Notch ligands. Notch-ligand binding to NOTCH1, -2, -3, or -4 receptor induces the receptor proteolysis by metalloprotease and γ-secretase to release Notch intracellular domain (NICD).
DLL1 [1] is the human homolog of the Drosophila Notch ligand Delta [2]. Sequence analysis predicted that the 723-aa DLL1 transmembrane protein, which is 88% identical to the mouse DLL1 protein [3], has a DSL domain followed by 8 tandem EGF-like repeats and a short cytoplasmic C-terminal region. A soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal aa inhibited the differentiation of myeloid progenitors but promoted their expansion [4]. DLL1 blocks the differentiation of progenitor cells into the B cell lineage while promoting the emergence of a population of cells with the characteristics of a T cell/NK cell precursor [5]. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM10 and represents a substrate for several other members of the ADAM family [6, 7]. The soluble 38kDa Delta1 intracellular domain (DICD) that is generated by the presenilin-dependent γ-secretase cleavage is transported into the nucleus and likely plays a role in transcriptional events [8-10]. Recently it has been shown that DLL1 is essential for postnatal arteriogenesis [11].
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link O00548: DLL1 (human) [Precursor]

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by AdipoGen, Inc.
General Literature References
[1] Human ligands of the Notch receptor: G.E. Gray, et al.; Am. J. Pathol. 154, 785 (1999) Abstract; Full Text
[2] The molecular genetics of early neurogenesis in Drosophila melanogaster: E. Knust and J.A. Campos-Ortega; Bioessays 11, 95 (1989) Abstract
[3] Transient and restricted expression during mouse embryogenesis of Dll1, a murine gene closely related to Drosophila Delta: B. Bettenhausen, et al.; Development 121, 2407 (1995) Abstract; Full Text
[4] A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells: W. Han, et al.; Blood 95, 1616 (2000) Abstract; Full Text
[5] Differential effects of Notch ligands Delta-1 and Jagged-1 in human lymphoid differentiation: A.C. Jaleco, et al.; J. Exp. Med. 194, 991 (2001) Abstract; Full Text
[6] The Notch ligand Delta1 is sequentially cleaved by an ADAM protease and gamma-secretase: E. Six, et al.; PNAS 100, 7638 (2003) Abstract; Full Text
[7] Proteolytic processing of delta-like 1 by ADAM proteases: E. Dyczynska, et al.; J. Biol. Chem. 282, 436 (2007) Abstract; Full Text
[8] Notch-induced proteolysis and nuclear localization of the Delta ligand: C.E. Bland, et al.; J. Biol. Chem. 278, 13607 (2003) Abstract; Full Text
[9] The Notch ligands, Delta1 and Jagged2, are substrates for presenilin-dependent "gamma-secretase" cleavage: T. Ikeuchi and S.S. Sisodia; J. Biol. Chem. 278, 7751 (2003) Abstract; Full Text
[10] The Notch ligands, Jagged and Delta, are sequentially processed by alpha-secretase and presenilin/gamma-secretase and release signaling fragments: M.J. LaVoie and D.J. Selkoe; J. Biol. Chem. 278, 34427 (2003) Abstract; Full Text
[11] The Notch Ligand Delta-Like 1 is Essential for Postnatal Arteriogenesis: A. Limbourg, et al.; Circ. Res. 100, 363 (2007) Abstract
[12] NOTCH signaling as a novel cancer therapeutic target: L. Miele, et al.; Curr. Cancer Drug Targets 6, 313 (2006) Abstract
[13] Notch signaling in gastrointestinal tract: M. Katoh; Int. J. Oncol. 30, 247 (2007), Review Abstract
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Recombinant Proteins / Fusion Proteins
 
 
ALX-201-386 Revised 26-May-08
DLL4 (human):Fc (human) (recombinant)
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SYNONYMS Delta-like Protein 4 (human):Fc (human) (recombinant)
Delta4 (human):Fc (human) (recombinant)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Delta-like [DLL] & Jagged / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-386-C010   10 µg 310.00 USD Add To Cart
ALX-201-386-C050   50 µg 860.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. Signal peptide and extracellular domain of recombinant human DLL4 (Delta-like protein 4; Delta4) (aa 1-529) is fused at the C-terminus to the Fc portion of human IgG1.
CONCENTRATION:

PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
Notch signalling pathways play key roles in cell-fate determination and differentiation in many tissues during embryonic and postnatal development. Notch ligands are divided into two subclasses, the delta and the serrate family [1]. DLL4 (Dll4) is a novel delta family member and exhibits all the hallmarks of the delta family of Notch ligands [2]. Human DLL4 shares 86% aa sequence identity with mouse Dll4. The extracellular region of the 685 aa protein contains 8 EGF-like repeats as well as a DSL (Delta, Serrate, Lag2) domain necessary for receptor binding. The protein also contains a transmembrane domain and a cytoplasmic tail lacking any catalytic motifs. DLL4 is largely restricted to the vascular endothelium and has been shown to function as a ligand for Notch1 and Notch4 [3]. Signalling through Notch1 regulates formation of tip cells during angiogenesis [4].
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9NR61: DLL4 (human) [Precursor]
MANUFACTURER Manufactured by AdipoGen, Inc.
General Literature References
[1] Human ligands of the Notch receptor: G.E. Gray, et al.; Am. J. Pathol. 154, 785 (1999) Abstract
[2] Dll4, a novel Notch ligand expressed in arterial endothelium: J.R. Shutter, et al.; Genes Dev. 14, 1313 (2000) Abstract
[3] Delta-like ligand 4 (Dll4) is induced by VEGF as a negative regulator of angiogenic sprouting: I.B. Lobov, et al.; PNAS 104, 3219 (2007) Abstract
[4] Dll4 signalling through Notch1 regulates formation of tip cells during angiogenesis: M. Hellstrom, et al.; Nature 445, 776 (2007) Abstract
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Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-390 Revised 27-Aug-08
Jagged-1 (human):Fc (human) (recombinant)
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SYNONYMS HJ1 (human):Fc (human) (recombinant)
CD339 (human):Fc (human) (recombinant)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Delta-like [DLL] & Jagged / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-390-C010   10 µg 295.00 USD Add To Cart
ALX-201-390-C050   50 µg 660.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. Signal peptide and extracellular domain of human Jagged-1 (HJ1; CD339) (aa 1-1067) are fused at the C-terminus to the Fc portion of human IgG.
CONCENTRATION: 0.5mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
General Information

The Notch family of proteins are involved in the regulation of key biological processes such as cell fate determination, proliferation, differentiation, and apoptosis. Jagged-1 (HJ1; CD339), a cell surface glycoprotein, is one of several ligands that activate multiple Notch receptors  and is involved in mediating Notch signalling. Jagged-1 signalling through Notch has been shown to play a role in hematopoiesis. Jagged-1 also seems to be involved in early and late stages of mammalian cardiovascular development. It has been shown to enhance fibroblast growth factor-induced angiogenesis (in vitro). Mutations in the human Jagged-1 gene cause Alagille syndrome.
Jagged-1 binds to Notch 1 receptors and activates Notch 1-dependent signalling pathways. For example, Jagged-1 inhibits T cell activation and is a promising therapeutically beneficial protein for multiple sclerosis or other autoimmune diseases. Additionally Jagged-1 was shown to block osteoclastogenesis in the presence of RANKL and M-CSF.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P78504: Jagged-1 [Precursor]
MANUFACTURER Manufactured by AdipoGen, Inc.
General Literature References
An antisense oligonucleotide to the notch ligand jagged enhances fibroblast growth factor-induced angiogenesis in vitro: A.B. Zimrin. et al.; J. Biol. Chem. 271, 32499 (1996) Abstract; Full Text
Mutations in the human Jagged1 gene are responsible for Alagille syndrome: T. Oda, et al.; Nat. Genet. 16, 235 (1997) Abstract
Expression of Notch-1 and its ligands, Delta-like-1 and Jagged-1, is critical for glioma cell survival and proliferation: B.W. Purow, et al.; Cancer Res. 65, 2353 (2005) Abstract; Full Text
Jagged1 protein enhances the differentiation of mesenchymal stem cells into cardiomyocytes: H. Li, et al.; BBRC 341, 320 (2006) Abstract
Up-regulation of gene related to anergy in lymphocytes is associated with Notch-mediated human T cell suppression: A.M. Kostianovsky, et al.; J. Immunol. 178, 6158 (2007) Abstract; Full Text
Endothelial expression of the Notch ligand Jagged1 is required for vascular smooth muscle development: F.A. High, et al.; PNAS 105, 1955 (2008) Abstract
NOTCH1 regulates osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblast lineage cells: S. Bai, et al.; J. Biol. Chem. 283, 6509 (2008) Abstract; Full Text
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Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • Angiogenesis Other Products
 
 
ALX-201-416 Revised 09-Jul-08
Dlk1 (mouse):Fc (human) (recombinant)
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SYNONYMS Delta-like Protein (DLK) (mouse):Fc (human) (recombinant)
Pref-1 (mouse):Fc (human) (recombinant)
Preadipocyte Factor 1 (mouse):Fc (human) (recombinant)
Fetal Antigen 1 (FA1) (mouse):Fc (human) (recombinant)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Adipokines Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-416-C010   10 µg 250.00 USD Add To Cart
ALX-201-416-C050   50 µg 570.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. The signal peptide and the extracellular domain of mouse Dlk1 (aa 1-305) is fused at the C-terminus to the Fc portion of human IgG.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q09163: DLK (mouse)
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • Delta-like [DLL] & Jagged / Related Products • Cell Differentiation
 
 
ALX-201-422 Revised 09-Jun-08 New product
DLL3 (human):Fc (human) (recombinant)
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SYNONYMS Delta-like Protein 3 (human):Fc (human) (recombinant)
Delta3 (human):Fc (human) (recombinant)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Delta-like [DLL] & Jagged / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-422-C010   10 µg 300.00 USD Add To Cart
ALX-201-422-C050   50 µg 830.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. Signal peptide and extracellular domain of recombinant human DLL3 (aa 1-466) is fused at the C-terminus to the Fc portion of human IgG1.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9NYJ7: DLL3(human) [Precursor]
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-425 Revised 24-Sep-08 New product
DLL1 (human):Fc (human) (recombinant)
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SYNONYMS Delta-like Protein 1 (human):Fc (human) (recombinant)
Delta1 (human):Fc (human) (recombinant)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Delta-like [DLL] & Jagged / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-425-C010   10 µg 230.00 USD Add To Cart
ALX-201-425-C050   50 µg 690.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. Signal peptide and extracellular domain of recombinant human DLL1 (Delta-like protein 1; Delta1) (aa 1-545) is fused at the C-terminus to the Fc portion of human IgG.
CONCENTRATION: 0.3mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
Notch signalling pathways play key roles in cell-fate determination and differentiation in many tissues during embryonic and postnatal development. Notch ligands are divided into two subclasses, the delta and the serrate family [1]. Delta-like 1, 3, and 4 (DLL1, -3, -4), JAG1 and JAG2 with DSL (Delta, Serrate, Lag2) domain are typical Notch ligands, while DNER, F3/Contactin and NB-3 without DSL domain are atypical Notch ligands. Notch-ligand binding to NOTCH1, -2, -3, or -4 receptor induces the receptor proteolysis by metalloprotease and γ-secretase to release Notch intracellular domain (NICD).
DLL1 [1] is the human homolog of the Drosophila Notch ligand Delta [2]. Sequence analysis predicted that the 723-aa DLL1 transmembrane protein, which is 88% identical to the mouse DLL1 protein [3], has a DSL domain followed by 8 tandem EGF-like repeats and a short cytoplasmic C-terminal region. A soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal aa inhibited the differentiation of myeloid progenitors but promoted their expansion [4]. DLL1 blocks the differentiation of progenitor cells into the B cell lineage while promoting the emergence of a population of cells with the characteristics of a T cell/NK cell precursor [5]. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM10 and represents a substrate for several other members of the ADAM family [6, 7]. The soluble 38kDa Delta1 intracellular domain (DICD) that is generated by the presenilin-dependent γ-secretase cleavage is transported into the nucleus and likely plays a role in transcriptional events [8-10]. Recently it has been shown that DLL1 is essential for postnatal arteriogenesis [11].
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link O00548: DLL1 (human) [Precursor]
MANUFACTURER Manufactured by AdipoGen, Inc.
General Literature References
[1] Human ligands of the Notch receptor: G.E. Gray, et al.; Am. J. Pathol. 154, 785 (1999) Abstract; Full Text
[2] The molecular genetics of early neurogenesis in Drosophila melanogaster: E. Knust and J.A. Campos-Ortega; Bioessays 11, 95 (1989) Abstract
[3] Transient and restricted expression during mouse embryogenesis of Dll1, a murine gene closely related to Drosophila Delta: B. Bettenhausen, et al.; Development 121, 2407 (1995) Abstract; Full Text
[4] A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells: W. Han, et al.; Blood 95, 1616 (2000) Abstract; Full Text
[5] Differential effects of Notch ligands Delta-1 and Jagged-1 in human lymphoid differentiation: A.C. Jaleco, et al.; J. Exp. Med. 194, 991 (2001) Abstract; Full Text
[6] The Notch ligand Delta1 is sequentially cleaved by an ADAM protease and gamma-secretase: E. Six, et al.; PNAS 100, 7638 (2003) Abstract; Full Text
[7] Proteolytic processing of delta-like 1 by ADAM proteases: E. Dyczynska, et al.; J. Biol. Chem. 282, 436 (2007) Abstract; Full Text
[8] Notch-induced proteolysis and nuclear localization of the Delta ligand: C.E. Bland, et al.; J. Biol. Chem. 278, 13607 (2003) Abstract; Full Text
[9] The Notch ligands, Delta1 and Jagged2, are substrates for presenilin-dependent "gamma-secretase" cleavage: T. Ikeuchi and S.S. Sisodia; J. Biol. Chem. 278, 7751 (2003) Abstract; Full Text
[10] The Notch ligands, Jagged and Delta, are sequentially processed by alpha-secretase and presenilin/gamma-secretase and release signaling fragments: M.J. LaVoie and D.J. Selkoe; J. Biol. Chem. 278, 34427 (2003) Abstract; Full Text
[11] The Notch Ligand Delta-Like 1 is Essential for Postnatal Arteriogenesis: A. Limbourg, et al.; Circ. Res. 100, 363 (2007) Abstract
[12] NOTCH signaling as a novel cancer therapeutic target: L. Miele, et al.; Curr. Cancer Drug Targets 6, 313 (2006) Abstract
[13] Notch signaling in gastrointestinal tract: M. Katoh; Int. J. Oncol. 30, 247 (2007), Review Abstract
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-427 Revised 09-Jul-08 New product
Dlk1 (human):Fc (human) (recombinant)
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SYNONYMS Delta-like Protein (DLK) (human):Fc (human) (recombinant)
Pref-1 (human):Fc (human) (recombinant)
Preadipocyte Factor 1 (human):Fc (human) (recombinant)
Fetal Antigen 1 (FA1) (human):Fc (human) (recombinant)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Adipokines Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-427-C010   10 µg 250.00 USD Add To Cart
ALX-201-427-C050   50 µg 660.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. The signal peptide and the extracellular domain of human Dlk1 (aa 1-303) is fused at the C-terminus to the Fc portion of human IgG.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P80370: DLK (human)
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • Delta-like [DLL] & Jagged / Related Products • Cell Differentiation
 
 
ALX-201-428