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ALX-350-014
Revised 03-Apr-08
Calyculin A
PRODUCT LINE
Natural Products / Antibiotics
PRODUCT CATEGORY
Natural Products - Other Signal Transduction Pathway Modulators
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-350-014-C010
10 µg
65.00 USD
ALX-350-014-C025
25 µg
110.00 USD
ALX-350-014-C050
50 µg
190.00 USD
ALX-350-014-C100
100 µg
320.00 USD
Product Specification
FORMULA:
C
50
H
81
N
4
O
15
P
MW:
1009.2
CAS NUMBER:
101932-71-2
SOURCE/HOST:
Isolated from
Discodermia calyx
.
PURITY:
≥95%
APPEARANCE:
Lyophilized.
SOLUBILITY:
Soluble in DMSO, 100% ethanol, methanol, acetonitrile, chloroform, methylene chloride or benzene. Insoluble in water.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
USE/STABILITY:
May by subject to isomerization when in solution. Biological activity is not affected by isomerization.
HANDLING:
Protect from light and moisture.
HAZARD:
TOXIC. MAY BE CARCINOGENIC.
Product Description
Potent cell permeable inhibitor with high specificity for protein phosphatase 1 (PP1) and 2A (PP2A). Inhibits p130cas tyrosine phosphorylation. Enhances the phosphorylation level of NF-κB. Affects intracellular signalling processes that require 14-3-3. Potent non-phorbol type tumor promoter. Prevents γ-radiation induced apoptosis.
Product Specific Literature References
Okadaic acid: an additional non-phorbol-12-tetradecanoate-13-acetate- type tumor promoter:
M. Suganuma, et al.; PNAS
85
, 1768 (1988)
Abstract
Protein phosphatases come of age:
P.Cohen & P.T. Cohen; J. Biol. Chem.
264
, 21435 (1989)
Abstract
;
Full Text
Calyculin A and okadaic acid: inhibitors of protein phosphatase activity:
H. Ishihara, et al.; BBRC
159
, 871 (1989)
Abstract
The structure and regulation of protein phosphatases:
P. Cohen; Ann. Rev. Biochem.
58
, 453 (1989)
Abstract
Calyculin A, an inhibitor of protein phosphatases, a potent tumor promoter on CD-1 mouse skin:
M. Suganuma, et al.; Cancer Res.
50
, 3521 (1990)
Abstract
Nonphorbol tumor promoters okadaic acid and calyculin-A induce membrane translocation of protein kinase C:
R. Gopalakrishna; BBRC
189
, 950 (1992)
Abstract
Comparative effects of protein phosphatase inhibitors (okadaic acid and calyculin A) on human leukemia HL60, HL60/ADR and K562 cells:
K. Sakurada, et al.; BBRC
187
, 488 (1992)
Abstract
Calyculin A, a potent inhibitor of phosphatases-1 and -2A, prevents apoptosis:
Q. Song & M.F. Lavin; BBRC
190
, 47 (1993)
Abstract
Enhancement of AMPA-mediated synaptic transmission by the protein phosphatase inhibitor calyculin A in rat hippocampal slices:
A. Figurov, et al.; Eur. J. Neurosci.
5
, 1035 (1993)
Abstract
Calyculin A, a non-phorbol ester type tumor promotor, induced oxidative DNA damage in stimulated human neutrophil-like cells:
T. Takeuchi, et al.; BBRC
205
, 1803 (1994)
Abstract
Potassium and calcium channel involvement in induction of long-lasting synaptic enhancement by calyculin A, a protein phosphatase inhibitor, in rat hippocampal CA1 region:
N. Murakami, et al.; Neurosci. Lett.
176
, 181 (1994)
Abstract
Differential toxicity of the protein phosphatase inhibitors microcystin and calyculin A:
M.T. Runnegar, et al.; J. Pharmacol. Exp. Ther.
273
, 545 (1995)
Abstract
Inhibition of p130cas tyrosine phosphorylation by calyculin A:
W. Qiu, et al.; J. Leukoc. Biol.
63
, 631 (1998)
Abstract
Unique features of the okadaic acid activity class of tumor promoters:
H. Fujiki & M. Suganuma; J. Cancer Res. Clin. Oncol.
125
, 150 (1999), Review
Abstract
Calyculin A-induced vimentin phosphorylation sequesters 14-3-3 and displaces other 14-3-3 partners in vivo:
G. Tzivion, et al.; J. Biol. Chem.
275
, 29772 (2000)
Abstract
Calyculin A induces apoptosis and stimulates phosphorylation of p65NF-kappaB in human osteoblastic osteosarcoma MG63 cells:
H. Tanaka, et al.; Int. J. Oncol.
31
, 389 (2007)
Abstract
Further Categories Containing This Product:
PP2A / Related Products
•
PP1 / Related Products
•
Natural Products - Other Tumor Promoters
•
14-3-3 Proteins / Related Products
•
Carcinogens & Tumor Promoters Other Products
•
NF-kB Pathway Modulators
•
Natural Products - Apoptosis Inducers & Inhibitors
•
Marine Natural Products
ALX-210-462
Revised 10-Apr-08
Polyclonal Antibody to TRB-3 (human)
SYNONYMS
anti-Tribbles Homolog 3 (human) PAb
anti-Neuronal Cell Death-inducible Putative Kinase (human) PAb
anti-p65-interacting Inhibitor of NF-κB (human) PAb
anti-SINK (human) PAb
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
NF-kB Pathway Modulators
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-462-C100
100 µg
310.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
PURITY DETAIL:
Purified IgG.
FORMULATION:
Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:
Synthetic peptide corresponding to aa 112-125 (A
112
RLPPHKHVARPTE
125
) of human TRB-3 (tribbles homolog 3).
SPECIFICITY:
Recognizes human TRB-3.
APPLICATION:
ELISA
(direct and indirect: 1:2’000-1:5’000)
Immunohistochemistry
(paraffin sections(1:500))
Western Blot
(1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 1-2 weeks when stored at +4°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
Tribbles homolog 3 (TRB-3) is a pseudokinase, shown to act as a potent negative regulator of PPARγ, a master regulator of adipocyte differentiation, and to control adipogenesis.
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
PPAR Other Products
•
PI(3)K-Akt-mTOR Pathway Other Products
•
MAPK Pathway Other Products
•
Polyclonal Antibodies
•
Transcription Regulators Other Products
ALX-270-458
Revised 15-Jan-08
PPM-18
SYNONYMS
2-Benzoylamino-1,4-naphthoquinone
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
NOS Inhibitors (NOS Induction & Enzyme Activity)
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-270-458-M010
10 mg
140.00 USD
Product Specification
FORMULA:
C
17
H
11
NO
3
MW:
277.3
CAS NUMBER:
65240-86-0
PURITY:
≥97% (HPLC)
APPEARANCE:
Yellow to orange solid.
SOLUBILITY:
Soluble in DMSO or acetonitrile.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 3 years after receipt when stored at -20°C.
HANDLING:
Packaged under inert gas. Protect from light. After reconstitution, prepare aliquots and store at -20°C.
Product Description
Cell permeable, anti-inflammatory agent that inhibits expression of iNOS (NOS II) (IC
50
~5mM). Blocks activation of NF-κB
in vitro
and
in vivo
. Does not directly affect enzymatic activities of iNOS (NOS II) or eNOS (NOS III).
Product Specific Literature References
Synthesis and antiplatelet, antiinflammatory and antiallergic activities of 2,3-disubstituted 1,4-naphthoquinones:
J.C. Lien, et al.; Chem. Pharm. Bull. (Tokyo)
44
, 1181 (1996)
Abstract
Inhibition of nitric oxide synthase expression by PPM-18, a novel anti-inflammatory agent, in vitro and in vivo:
S.M. Yu, et al.; Biochem. J.
328
, 363 (1997)
Abstract
;
Full Text
Shear stress regulates endothelial nitric-oxide synthase promoter activity through nuclear factor kappaB binding:
M.E. Davis, et al.; J. Biol. Chem.
279
, 163 (2004)
Abstract
;
Full Text
Further Categories Containing This Product:
NF-kB Pathway Modulators
•
Anti-inflammatory Agents Other Products
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