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Phosphatases / Related Products
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ALX-165-020 Revised 21-Feb-03
Protein Tyrosine Phosphatase Substrate
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SYNONYMS Tyrosine Phosphatase Substrate
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Protein Tyrosine Phosphatases Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-165-020-M001   1 mg 140.00 USD Add To Cart
ALX-165-020-M005   5 mg 560.00 USD Add To Cart
Product Specification
SEQUENCE: H-Thr-Glu-Pro-Glu-Tyr(PO3H2)-Gln-Pro-Gly-Glu-NH2
FORMULA: C45H66N11O21P
MW: 1128.1
PURITY: ≥97%
APPEARANCE: White lyophilized powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Substrate for protein tyrosine phosphatases, suitable for continuous fluorimetric assays, based on the effect of phosphate substitution on the intrinsic fluorescence of the dephosphorylated peptide.
Product Specific Literature References
Synthesis and fluorescence properties of a substrate for a continuous fluorimetric assay of protein tyrosine phosphatases: C. Garcia-Echeverria & D.H. Rich; Bioorg. Med. Chem. Lett. 3, 1601 (1993)
Further Categories Containing This Product:
Peptides
 
 
ALX-202-034 Revised 13-Dec-04
Calcineurin (bovine brain)
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SYNONYMS Calmodulin Binding Protein (bovine brain)
Protein Phosphatase 2B (bovine brain)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PP2B [Calcineurin] / Related Products
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ALX-202-034-U100   100 U 590.00 USD Add To Cart
Product Specification
CAS NUMBER: 9025-75-6
EC: 3.1.3.16
MERCK INDEX: 14: 1640
SOURCE/HOST: Isolated from bovine brain.
FORMULATION: Lyophilized powder containing ~1% protein (Lowry) and 0.5% EGTA, buffer salts and stabilizers.
SPECIFIC ACTIVITY: ~2'500-5'000U/mg protein. One unit causes a 50% inhibition of the activated phosphodiesterase activity when assayed with 2 units of activator and 0.1mM Ca2+ in an enzyme coupled system at 30°C, pH 7.5.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Enzymes • Natural Proteins • Nitric Oxide Pathway Other Products
 
 
ALX-805-041 Revised 04-Jan-06
Monoclonal Antibody to CD45 (human) (MEM-28)
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PRODUCT LINE Immunology
PRODUCT CATEGORY CD45 / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-805-041-C100   100 µg 195.00 USD Add To Cart
ALX-805-041F-T100 FITC 100 tests 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: MEM-28
ISOTYPE: Mouse IgG1
CONCENTRATION: MEM-28: 1mg/ml.
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: MEM-28: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
MEM-28-FITC: Liquid. In PBS containing 15mM sodium azide and 0.2% BSA.
IMMUNOGEN: Human thymocytes and T lymphocytes.
SPECIFICITY: Recognizes all isoforms of human CD45.
APPLICATION: MEM-28:
Flow Cytometry
Immunohistochemistry (paraffin sections - no pre-treatment of tissue sections)
Immunoprecipitation
Western Blot
MEM-28-FITC:
Flow Cytometry (use 20µl for 100µl of whole blood or 106 cells in a suspension)
SHIPPING: SHIPPED ON BLUE ICE
USE/STABILITY: MEM-28: Long Term Storage: -20°C. Avoid freeze/thaw cycles.
MEM-28-FITC: Long Term Storage: +4°C. Do not freeze. Protect from light.
Product Specific Literature References
Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen: V. Horejsi, et al.; Folia Biol (Praha) 32, 12 (1986) Abstract
Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74): V. Horejsi, et al.; Folia Biol (Praha) 34, 23 (1988) Abstract
Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules: L. Cermak, et al.; J. Biol. Chem. 277, 7955 (2002) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-805-067 Revised 19-Jun-07
Monoclonal Antibody to CD45RA (human) (MEM-56)
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PRODUCT LINE Immunology
PRODUCT CATEGORY CD45 / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-805-067-C100   100 µg 195.00 USD Add To Cart
ALX-805-067F-T100 FITC 100 tests 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: MEM-56
ISOTYPE: Mouse IgG2b
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE)
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: ALX-805-067-C100: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
ALX-805-067F-T100: Liquid. In PBS, pH 7.4, containing 0.2% BSA and 15mM sodium azide.
IMMUNOGEN: Human thymocytes and T lymphocytes.
SPECIFICITY: Recognizes human CD45RA.
APPLICATION: Flow Cytometry (20µl per 100µl of whole blood or 1 x 106 cells)
Immunohistochemistry (paraffin sections - pre-treatment not required)
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: ALX-805-067-C100: Avoid freeze/thaw cycles. After opening prepare aliquots and store at -20°C.
ALX-805-067F-T100: Store at +4°C. Do not freeze. Protect from light.
Product Specific Literature References
Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102: V. Bazil, et al.; Folia Biol (Praha) 35, 289 (1989) Abstract
Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression: V. Bazil, et al.; Immunogenetics 29, 202 (1989) Abstract
General Information
Human CD45RA is expressed on all cells of hematopoietic origin, except erythrocytes. CD45RA is a transmembrane tyrosine phosphate which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RA isoform predominates on naive/resting T cells and medullary thymocytes.
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-805-068 Revised 07-Nov-05
Monoclonal Antibody to CD45RB (human) (MEM-55)
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PRODUCT LINE Immunology
PRODUCT CATEGORY CD45 / Related Products
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ALX-805-068-C100   100 µg 195.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: MEM-55
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE)
PURITY DETAIL: Protein A affinity-purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Human thymocytes and T lymphocytes.
SPECIFICITY: Recognizes a sialidase-sensitive epitope of CD45RB.
APPLICATION: Flow Cytometry
Immunohistochemistry (paraffin sections - pre-treatment of tissue sections is not required)
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted exp: V. Horejsi, et al.; Folia Biol (Praha) 34, 23 (1988) Abstract
Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression: V. Bazil, et al.; Immunogenetics 29, 202 (1989) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-805-069 Revised 21-Jul-03
Monoclonal Antibody to CD45RB (human) (MEM-143)
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PRODUCT LINE Immunology
PRODUCT CATEGORY CD45 / Related Products
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ALX-805-069-C100   100 µg 195.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: MEM-143
ISOTYPE: Mouse IgG1
IMMUNOGEN: Human peripheral blood lymphocytes.
SPECIFICITY: Recognizes human CD45RB. Reacts with a protein determinant of CD45RB and is therefore not neuraminidase sensitive like most other MAbs to CD45RB.
APPLICATION: Flow Cytometry.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-569 Revised 24-Oct-06
Monoclonal Antibody to PP2A (Subunit B) (PR55) (α Isoform) (2G9)
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SYNONYMS anti-Protein Phosphatase 2A (Subunit B) (PR55) (α Isoform) MAb (2G9)
anti-PP2A B/PR55α MAb (2G9)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PP2A / Related Products
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ALX-804-569-C100   100 µg 330.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 2G9
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 398-411 (C398ASGKRKKDEISVD411) of rat PP2A (subunit B) (PR55) (α isoform). This sequence is completely conserved in human, mouse, rat, pig, rabbit, chicken and xenopus.
SPECIFICITY: Recognizes human, mouse and rat PP2A (subunit B) (PR55) (α isoform). Cross-reactivity with other species has not been tested. Detects a band of ~50kDa by Western blot. Cross-reacts with mouse and rat PP2A (subunit B) (PR55) (δ isoform).
CROSSREACTIVITY PROTEINS: PP2A (subunit B) (PR55) (δ isoform)
APPLICATION: Western Blot (1:1'000-1:10'000)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Protein phosphatase 2A associates with and regulates atypical PKC and the epithelial tight junction complex: V. Nunbhakdi-Craig, et al.; J. Cell Biol. 158, 967 (2002) Abstract; Full Text
Identification of a subunit of a novel Kleisin-beta/SMC complex as a potential substrate of protein phosphatase 2A: F.M. Yeong, et al.; Curr. Biol. 13, 2058 (2003) Abstract
General Literature References
Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling: V. Janssens and J. Goris; Biochem J. 353, 417 (2001) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-636 Revised 31-Oct-06
Monoclonal Antibody to PP2A (Subunit A) (PR65) (α Isoform) (4C10)
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SYNONYMS anti-Protein Phosphatase 2A (Subunit A) (PR65) (α Isoform) MAb (4C10)
anti-PP2A A/PR65α MAb (4C10)
anti-Medium Tumor Antigen-associated 61kDa Protein MAb (4C10)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PP2A / Related Products
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ALX-804-636-C050   50 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 4C10
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human PP2A (subunit A) (PR65) (α isoform) (aa 1-168).
SPECIFICITY: Recognizes human and mouse PP2A (subunit A) (PR65) (α isoform). Recognizes mouse PP2A (subunit A) (PR65) (β isoform) (weak).
CROSSREACTIVITY PROTEINS: PP2A (subunit A) (PR65) (β isoform) (weak)
APPLICATION: Western Blot (1:5’000)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
General Information
The regulatory A subunit (PR65) is tightly associated with catalytic subunit (PP2Ac), forming a scaffold to which the appropriate B subunit can bind. Different B subunits interact via the same or overlapping sites within the A subunit of the core dimer, which explains why binding of the B subunits is mutually exclusive. Two distinct PR65 isoforms (α and β) which share 86% sequence identity are ubiquitiously expressed. Recent studies reported that the genes encoding both the α and β isoforms of PR65/A were altered at low frequency in a variety of primary human tumors, identifying these subunits as tumorsuppressors. Partial or total loss (by cleavage through caspase-3) of the PR65/A subunit resulted in an increase in PP2A activity towards specific pro-apoptotic substrates involved in the cellular apoptotic response.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-811 Revised 06-Jun-06
Monoclonal Antibody to Acidic Nuclear Phosphoprotein 32A (human) (RJ1)
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SYNONYMS anti-HLA-DR-associated Protein 1 (human) MAb (RJ1)
anti-PHAPI (human) MAb (RJ1)
anti-Acidic Nuclear Phosphoprotein pp32 (human) MAb (RJ1)
anti-PP2A Inhibitor Protein 1α (human) MAb (RJ1)
anti-I1αPP2A (human) MAb (RJ1)
anti-LANP (human) MAb (RJ1)
anti-ANP32A (human) MAb (RJ1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Protein Serine / Threonine Phosphatases Other Products
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ALX-804-811-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: RJ1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human acidic nuclear phosphoprotein 32A.
SPECIFICITY: Recognizes human acidic nuclear phosphoprotein 32A.
APPLICATION: Immunocytochemistry (1:100-1:200; works on endogenous)
Immunoprecipitation (works on endogenous)
Western Blot (1:4'000; works on endogenous)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Granzyme A activates an endoplasmic reticulum-associated caspase-independent nuclease to induce single-stranded DNA nicks: P.J. Beresford, et al.; J. Biol. Chem. 276, 43285 (2001) Abstract; Full Text
HMG2 interacts with the nucleosome assembly protein SET and is a target of the cytotoxic T-lymphocyte protease granzyme A: Z. Fan, et al.; Mol. Cell. Biol. 22, 2810 (2002) Abstract; Full Text
Cleaving the oxidative repair protein Ape1 enhances cell death mediated by granzyme A: Z. Fan, et al.; Nat. Immunol. 4, 145 (2003) Abstract
Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor: Z. Fan, et al.; Cell 112, 659 (2003) Abstract
Granzyme a induces caspase-independent mitochondrial damage, a required first step for apoptosis: D. Martinvalet, et al.; Immunity 22, 355 (2005) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-254 Revised 14-Aug-07
Monoclonal Antibody to PTEN (6H2.1)
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SYNONYMS anti-Phosphatase and Tensin Homolog Deleted on Chromosome 10 MAb (6H2.1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PTEN / Related Products
Ordering Information
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ALX-804-254-C100   100 µg 480.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 6H2.1
ISOTYPE: Mouse IgG
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Protein A-affinity purified. Sterile filtered.
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Recombinant human PTEN.
SPECIFICITY: Recognizes human, mouse and rat PTEN (epitope aa 304-403). Detects a band of ~60kDa by Western blot.
APPLICATION: ELISA
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation (5µg)
Western Blot (1-5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
POSITIVE CONTROL: For Positive Control in Western blot see Prod. No. ALX-201-079.
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Product Specific Literature References
Immunohistochemical evidence of loss of PTEN expression in primary ductal adenocarcinomas of the breast: A. Perren, et al.; Am. J. Pathol. 155, 1253 (1999) Abstract; Full Text
PTEN suppresses breast cancer cell growth by phosphatase activity- dependent G1 arrest followed by cell death: L.P. Weng, et al.; Cancer Res. 59, 5808 (1999) Abstract; Full Text
Altered PTEN expression as a diagnostic marker for the earliest endometrial precancers: G.L. Mutter, et al.; J. Natl. Cancer Inst. 92, 924 (2000) Abstract
Changes in endometrial PTEN expression throughout the human menstrual cycle: G.L. Mutter, et al.; J. Clin. Endocrinol. Metab. 85, 2334 (2000) Abstract; Full Text
Molecular identification of latent precancers in histologically normal endometrium: G.L. Mutter, et al.; Cancer Res. 61, 4311 (2001) Abstract; Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products • Monoclonal Antibodies
 
 
ALX-804-451 Revised 14-Aug-07
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Monoclonal Antibody to PTEN (11G8.1)
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SYNONYMS anti-Phosphatase and Tensin Homolog Deleted on Chromosome 10 MAb (11G8.1)