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ALX-804-530
Revised 14-Aug-07
Monoclonal Antibody to Caspase-1 (mouse) (1H11)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-530-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
1H11
ISOTYPE:
Rat IgG1
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:
p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T
206
ALEMVKEVKEFAAC
220
) of mouse caspase-1.
SPECIFICITY:
Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION:
ELISA
Flow Cytometry
Western Blot
Other applications not tested.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death:
V.S. Marsden, et al.; Blood
107
, 1872 (2006)
Abstract
;
Full Text
General Information
Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release.
General Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins
:
L.A. O'Reilly, et al.; Biotechniques
25
, 824 (1998), Methodology
Abstract
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9
:
L.A. O'Reilly, et al.; Cell Death Differ.
9
, 832 (2002), Methodology
Abstract
Further Categories Containing This Product:
Inflammasome
•
Monoclonal Antibodies
ALX-804-531
Revised 10-Mar-06
Monoclonal Antibody to Caspase-1 (mouse) (4G8)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-531-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
4G8
ISOTYPE:
Rat IgG1
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:
p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T
206
ALEMVKEVKEFAAC
220
) of mouse caspase-1.
SPECIFICITY:
Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION:
ELISA
Flow Cytometry
Western Blot
Other applications not tested.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
Product Images
Please click on thumbnails to enlarge.
General Information
Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release.
General Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins
:
L.A. O'Reilly, et al.; Biotechniques
25
, 824 (1998), Methodology
Abstract
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9
:
L.A. O'Reilly, et al.; Cell Death Differ.
9
, 832 (2002), Methodology
Abstract
Further Categories Containing This Product:
Inflammasome
•
Monoclonal Antibodies
ALX-804-803
Revised 26-Oct-07
Monoclonal Antibody to NALP1 (human) (Nalpy1-4)
SYNONYMS
anti-NACHT-, LRR- and PYD-containing Protein 1 (human) MAb (Nalpy1-4)
anti-DEFCAP (human) MAb (Nalpy1-4)
anti-NAC (human) MAb (Nalpy1-4)
anti-CARD7 (human) MAb (Nalpy1-4)
anti-Caspase Recruitment Domain-containing Protein 7 (human) MAb (Nalpy1-4)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Inflammasome
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-803-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
Nalpy1-4
ISOTYPE:
Mouse IgG1
SOURCE/HOST:
Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human NALP1 (NACHT-, LRR- and PYD-containing protein 1) (pyrin domain).
SPECIFICITY:
Recognizes the pyrin domain (PYD) of human NALP1. Does not cross-react with mouse NALP1.
APPLICATION:
Immunocytochemistry (1:1'000)
Immunohistochemistry (frozen sections, paraffin sections (microwave treatment))
Immunoprecipitation
Western Blot (1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
POSITIVE CONTROL:
For Positive Control (Western blot) see Prod. No.
ALX-840-603
.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response:
J.A. Kummer, et al.; J. Histochem. Cytochem.
55
, 443 (2007)
Abstract
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products
•
Monoclonal Antibodies
ALX-804-818
Revised 01-Mar-07
Monoclonal Antibody to NALP3 (human) (Nalpy3-a)
SYNONYMS
anti-NACHT-, LRR- and PYD-containing Protein 3 (human) MAb (Nalpy3-a)
anti-Cryopyrin (human) MAb (Nalpy3-a)
anti-Cold Autoinflammatory Syndrome 1 Protein (human) MAb (Nalpy3-a)
anti-PYPAF1 (human) MAb (Nalpy3-a)
anti-PYRIN-containing APAF1-like Protein 1 (human) MAb (Nalpy3-a)
anti-CIAS1 (human) MAb (Nalpy3-a)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Inflammasome
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-818-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
Nalpy3-a
ISOTYPE:
Mouse IgG1
SOURCE/HOST:
Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human NALP3 (NACHT-, LRR- and PYD-containing protein 3) (pyrin domain).
SPECIFICITY:
Recognizes human NALP3. Detects endogenous protein by ICC and WB.
APPLICATION:
Immunocytochemistry (1:100)
Western Blot (1:1’000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder
:
L. Agostini, et al.; Immunity
20
, 319 (2004)
Abstract
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation
:
M. Chamaillard, et al.; Cell. Microbiol.
5
, 581 (2003)
Abstract
;
Full Text
Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties
:
W. O'Connor, Jr., et al.; J. Immunol.
171
, 6329 (2003)
Abstract
Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases
:
F. Martinon and J. Tschopp; Cell
117
, 561 (2004)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-819
Revised 26-Oct-07
Monoclonal Antibody to NALP3 (human) (Nalpy3-b)
SYNONYMS
anti-NACHT-, LRR- and PYD-containing Protein 3 (human) MAb (Nalpy3-b)
anti-Cryopyrin (human) MAb (Nalpy3-b)
anti-Cold Autoinflammatory Syndrome 1 Protein (human) MAb (Nalpy3-b)
anti-PYPAF1 (human) MAb (Nalpy3-b)
anti-PYRIN-containing APAF1-like Protein 1 (human) MAb (Nalpy3-b)
anti-CIAS1 (human) MAb (Nalpy3-b)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Inflammasome
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-819-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
Nalpy3-b
ISOTYPE:
Mouse IgG1
SOURCE/HOST:
Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human NALP3 (NACHT-, LRR- and PYD-containing protein 3) (pyrin domain).
SPECIFICITY:
Recognizes human NALP3. Detects endogenous protein by IP and WB.
APPLICATION:
Immunohistochemistry (frozen sections)
Immunoprecipitation (1:50)
Western Blot (1:1’000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder
:
L. Agostini, et al.; Immunity
20
, 319 (2004)
Abstract
Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response:
J.A. Kummer, et al.; J. Histochem. Cytochem.
55
, 443 (2007)
Abstract
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation
:
M. Chamaillard, et al.; Cell. Microbiol.
5
, 581 (2003)
Abstract
;
Full Text
Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties
:
W. O'Connor, Jr., et al.; J. Immunol.
171
, 6329 (2003)
Abstract
Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases
:
F. Martinon and J. Tschopp; Cell
117
, 561 (2004)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-139
Revised 09-May-07
Monoclonal Antibody to RIP2 (human) (Nick-1)
SYNONYMS
anti-Receptor-interacting Protein 2 (human) MAb (Nick-1)
anti-CARDIAK (human) MAb (Nick-1)
anti-CARD-containing Interleukin-1β Converting Enzyme Associated Kinase (human) MAb (Nick-1)
anti-RICK (human) MAb (Nick-1)
anti-RIPK2 (human) MAb (Nick-1)
anti-Receptor-interacting Serine/Threonine Protein Kinase 2 (human) MAb (Nick-1)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
RIP Kinases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-139-C100
100 µg
360.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
Nick-1
ISOTYPE:
Rat IgG2a
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human RIP2 (receptor-interacting protein 2) (aa 1-322).
SPECIFICITY:
Recognizes human RIP2.
APPLICATION:
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored as recommended.
POSITIVE CONTROL:
For Positive Control (Western blot) see Prod. No.
ALX-840-604
.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Participation of Rip2 in LPS signaling is independent of its kinase activity
:
C. Lu, et al.; J. Biol. Chem.
280
, 16278 (2005)
Abstract
;
Full Text
PIDD mediates NF-kB activation in Response to DNA damage:
Janssens, et al.; Cell
123
, 1079 (2005)
Abstract
Participation of Rip2 in lipopolysaccharide signaling is independent of its kinase activity:
C. Lu, et al.; J. Biol. Chem.
280
, 16278 (2005)
Abstract
;
Full Text
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Nod-complex / Related Products
•
Caspase Recruitment Domain [CARD] Proteins / Related Products
•
Monoclonal Antibodies
ALX-804-155
Revised 27-Feb-07
Monoclonal Antibody to RIP3 (human) (Rippy-3)
SYNONYMS
anti-Receptor-interacting Protein 3 (human) MAb (Rippy-3)
anti-Receptor-interacting Serine/Threonine Protein Kinase 3 (human) MAb (Rippy-3)
anti-RIP-like Protein Kinase 3 (human) MAb (Rippy-3)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
RIP Kinases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-155-C100
100 µg
360.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
Rippy-3
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human RIP3 (receptor-interacting protein 3).
SPECIFICITY:
Recognizes human RIP3.
APPLICATION:
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:
Avoid freeze/thaw cycles.
POSITIVE CONTROL:
For Positive Control (Western blot) see Prod. No.
ALX-840-608
.
Product Images
Please click on thumbnails to enlarge.
General Information
Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNF-R1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3 [1-3]. RIP3 contains an N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNF-R1, mediates TNF-R1 induced apoptosis, and attenuates RIP and TNF-R1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues [2,3]. Meanwhile RIP4 has been identified and initially characterized. These recent data suggest that RIP4 is involved in NF-κB and JNK signalling and that caspase-dependent processing of RIP4 may negatively regulate NF-κB-dependent pro-survival or pro-inflammatory signals [4].
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
[1]
Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB
:
P.W. Yu, et al.; Curr. Biol.
9
, 539 (1999)
Abstract
[2]
RIP3, a novel apoptosis-inducing kinase
:
X. Sun, et al.; J. Biol. Chem.
274
, 16871 (1999)
Abstract
;
Full Text
[3]
Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB
:
N.J. Pazdernik, et al.; Mol. Cell. Biol.
19
, 6500 (1999)
Abstract
;
Full Text
[4]
RIP4 (DIK/PKK), a novel member of the RIP kinase family, activates NF-kappa B and is processed during apoptosis
:
E. Meylan, et al.; EMBO Rep.
3
, 1201 (2002)
Abstract
;
Full Text
Related Products
ALX-804-139
Monoclonal Antibody to RIP2 (human) (Nick-1)
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-209
Revised 27-May-08
Monoclonal Antibody to IκBα (6A920)
SYNONYMS
anti-NF-κB Inhibitor α MAb (6A920)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Inhibitors of kappa B [IkB] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-209-C100
100 µg
293.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
6A920
ISOTYPE:
Mouse IgG1
CONCENTRATION:
0.5mg/ml
PURITY DETAIL:
Protein G affinity-purified.
FORMULATION:
Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN:
Recombinant human IκBα
(aa 32-391).
SPECIFICITY:
Recognizes both phosphorylated and non-phosphorylated forms of human and mouse IκBα. Detects a band of ~40kDa by Western blot.
APPLICATION:
Immunoprecipitation (1µg/ml)
Western Blot (1-2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 6 months when stored at +4°C.
Product Images
Please click on thumbnails to enlarge.
General Literature References
Two waves of nuclear factor kappaB recruitment to target promoters
:
S. Saccani, et al.; J. Exp. Med.
193
, 1351 (2001)
Abstract
;
Full Text
p38-Dependent marking of inflammatory genes for increased NF-kappa B recruitment
:
S. Saccani, et al.; Nat. Immunol.
3
, 69 (2002)
Abstract
Modulation of NF-kappaB activity by exchange of dimers
:
S. Saccani, et al.; Mol. Cell
11
, 1563 (2003)
Abstract
PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock
:
T. Ikezoe, et al.; Mol. Pharmacol.
64
, 1521 (2003)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-235
Revised 31-Oct-06
Monoclonal Antibody to IκBα (human) (phosphorylated) (pSer32/36) (39A1431)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Inhibitors of kappa B [IkB] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-235-C100
100 µg
319.00 USD
