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ALX-201-056
Revised 21-Jul-08
Caspase-1 (active) (human) (recombinant)
SYNONYMS
Interleukin 1
β Converting Enzyme (active) (human) (recombinant)
IL-1β Converting Enzyme (active) (human) (recombinant)
ICE (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-056-U025
25 U
231.00 USD
ALX-201-056-U100
100 U
385.00 USD
Product Specification
EC:
3.4.22.36
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
~20’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-
p
NA (
p
NA:
p
-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
QUALITY CONTROL:
Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD-
p
NA (Prod. No.
ALX-260-026
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P29466:
Caspase-1 (human) (precursor)
AfCS Signalling Gateway link A000492:
Caspase-1 (mouse)
Further Categories Containing This Product:
Inflammasome
•
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-094
Revised 03-May-06
Caspase-5 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-094-U025
25 U
203.00 USD
ALX-201-094-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.-
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
In combination with caspase-5 activity assays, the enzyme is useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P51878:
Caspase-5 (human) (precursor)
AfCS Signalling Gateway link A000500:
Caspase-5 (mouse)
Further Categories Containing This Product:
Inflammasome
•
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-210-017
Revised 12-Apr-07
Polyclonal Antibody to NALP1 (human) (CT) (Bur 242)
SYNONYMS
anti-NACHT-, LRR- and PYD-containing Protein 1 (human) (CT) PAb (Bur 242)
anti-DEFCAP (human) (CT) PAb (Bur 242)
anti-NAC (human) (CT) PAb (Bur 242)
anti-CARD7 (human) (CT) PAb (Bur 242)
anti-Caspase Recruitment Domain-containing Protein 7 (human) (CT) PAb (Bur 242)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Inflammasome
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-017-R050
50 µl
190.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Antiserum containing PBS and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 1058-1077 (C
1058
VPSPASQGDLHTKPLGTDD
1077
) of C-terminal human NALP1 (NACHT-, LRR- and PYD-containing protein 1).
SPECIFICITY:
Recognizes human NALP1. Does not cross-react with mouse NALP1.
APPLICATION:
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
POSITIVE CONTROL:
For Positive Control (Western blot) see Prod. No.
ALX-840-603
.
Product Specific Literature References
A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis
:
Z.L. Chu, et al.; J. Biol. Chem.
276
, 9239 (2001)
Abstract
;
Full Text
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products
•
Polyclonal Antibodies
ALX-210-018
Revised 12-Apr-07
Polyclonal Antibody to NALP1 (human) (NT) (Bur 241)
SYNONYMS
anti-NACHT-, LRR- and PYD-containing Protein 1 (human) (NT) PAb (Bur 241)
anti-DEFCAP (human) (NT) PAb (Bur 241)
anti-NAC (human) (NT) PAb (Bur 241)
anti-CARD7 (human) (NT) PAb (Bur 241)
anti-Caspase Recruitment Domain-containing Protein 7 (human) (NT) PAb (Bur 241)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Inflammasome
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-018-R050
50 µl
190.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Antiserum containing PBS and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 161-180 (P
161
SSPDHESPSQESPNAPTST
180
) of N-terminal human NALP1 (NACHT-, LRR- and PYD-containing protein 1).
SPECIFICITY:
Recognizes human NALP1. Does not cross-react with mouse NALP1.
APPLICATION:
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
POSITIVE CONTROL:
For Positive Control (Western blot) see Prod. No.
ALX-840-603
.
Product Specific Literature References
A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis
:
Z.L. Chu, et al.; J. Biol. Chem.
276
, 9239 (2001)
Abstract
;
Full Text
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products
•
Polyclonal Antibodies
ALX-210-804
Revised 09-Oct-08
Polyclonal Antibody to Caspase-1
SYNONYMS
anti-Interleukin-1β Converting Enzyme PAb
anti-IL-1β Converting Enzyme PAb
anti-ICE PAb
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-804-C100
100 µg
315.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST:
From rabbit.
CONCENTRATION:
0.2mg/ml.
PURITY DETAIL:
Epitope-affinity purified.
FORMULATION:
Liquid. In PBS, pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
IMMUNOGEN:
Synthetic peptide corresponding to aa 129-152 of human caspase-1.
SPECIFICITY:
Recognizes human, mouse and rat caspase-1. Detects bands of ~45kDa (procaspase-1) and ~20kDa (cleavage product) by Western blot.
APPLICATION:
Immunocytochemistry (10-20µg/ml)
Immunoprecipitation (4-8µg/ml)
Western Blot (0.5-4µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Inflammasome
•
Polyclonal Antibodies
ALX-210-809
Revised 19-May-08
Polyclonal Antibody to Caspase-5
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-809-C100
100 µg
273.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Monkey
Others
SOURCE/HOST:
From rabbit.
CONCENTRATION:
0.2mg/ml
PURITY DETAIL:
Epitope-affinity purified.
FORMULATION:
Liquid. In PBS, pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
IMMUNOGEN:
Synthetic peptide surrounding aa 162 of human caspase-5.
SPECIFICITY:
Recognizes human, mouse, rat, hamster, monkey, rabbit and bovine caspase-5. Preferentially detects the intermediate (32kDa) and the fully cleaved (20kDa) caspase-5 by Western blot.
APPLICATION:
Western Blot (0.5-4µg/ml).
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Inflammasome
•
Polyclonal Antibodies
ALX-210-904
Revised 25-Jan-08
Polyclonal Antibody to NALP1 (human) (AL176)
SYNONYMS
anti-NACHT-, LRR- and PYD-containing Protein 1 (human) PAb (AL176)
anti-DEFCAP (human) PAb (AL176)
anti-NAC (human) PAb (AL176)
anti-CARD7 (human) PAb (AL176)
anti-Caspase Recruitment Domain-containing Protein 7 (human) PAb (AL176)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Inflammasome
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-904-R100
100 µl
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
PURITY:
Affinity purified.
FORMULATION:
Liquid. Neat serum.
IMMUNOGEN:
Synthetic peptide corresponding to aa 2-25 (A
2
GGAWGRLACYLEFLKKEELKEFQ
25
) of N-terminal human NALP1 (NACHT-, LRR- and PYD-containing protein 1).
SPECIFICITY:
Recognizes human NALP1. Does not cross-react with mouse NALP1.
APPLICATION:
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored as recommended.
HANDLING:
Avoid freeze/thaw cycles.
POSITIVE CONTROL:
For Positive Control (Western blot) see Prod. No.
ALX-840-603
.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta
:
F. Martinon; Mol. Cell.
10
, 417 (2002)
Abstract
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
Signalling: Initiation of pro-IL-1beta processing
:
E. Bell; Nat. Rev. Immunol.
2
, 719 (2002), (Review)
Abstract
The PYRIN-CARD protein ASC is an activating adaptor for caspase-1
:
S.M. Srinivasula, et al.; J. Biol. Chem.
277
, 21119 (2002)
Abstract
NALPS: a novel protein family involved in inflammation
:
J. Tschopp, et al.; Nat. Rev. Mol. Cell Biol.
4
, 95 (2003), (Review)
Abstract
;
Full Text
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products
•
Polyclonal Antibodies
ALX-210-905
Revised 23-May-08
Polyclonal Antibody to Asc (AL177)
SYNONYMS
anti-Pycard PAb (AL177)
anti-Apoptosis-associated Speck-like Protein Containing CARD PAb (AL177)
anti-TMS1 PAb (AL177)
anti-Target of Methylation-induced Silencing 1 PAb (AL177)
anti-CARD5 PAb (AL177)
anti-Caspase Recruitment Domain-containing Protein 5 PAb (AL177)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Inflammasome
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-905-R100
100 µl
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml
PURITY DETAIL:
Epitope-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 2-27 (G
2
RARDAILDALENLTAEELKKFKLKL
27
) of N-terminal human Asc (apoptosis-associated speck-like protein containing CARD; Pycard).
SPECIFICITY:
Recognizes human and mouse Asc.
APPLICATION:
Immunocytochemistry
Immunoprecipitation
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored as recommended.
HANDLING:
Avoid freeze/thaw cycles.
POSITIVE CONTROL:
For Positive Control (Western blot) see Prod. No.
ALX-840-602
.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta
:
F. Martinon, et al.; Mol. Cell.
10
, 417 (2002)
Abstract
NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder
:
L. Agostini, et al.; Immunity
20
, 319 (2004)
Abstract
P2X7 Receptor Differentially Couples to Distinct Release Pathways for IL-1{beta} in Mouse Macrophage:
P. Pelegrin, et al.; J. Immunol.
180
, 7147 (2008)
Abstract
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
Signalling: Initiation of pro-IL-1beta processing
:
E. Bell; Nat. Rev. Immunol.
2
, 719 (2002)
Abstract
The PYRIN-CARD protein ASC is an activating adaptor for caspase-1
:
S.M. Srinivasula, et al.; J. Biol. Chem.
277
, 21119 (2002)
Abstract
NALPS: a novel protein family involved in inflammation
:
J. Tschopp, et al.; Nat. Rev. Mol. Cell Biol.
4
, 95 (2003), (Review)
Abstract
;
Full Text
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins / Related Products
•
Polyclonal Antibodies
ALX-210-946
Revised 10-Oct-07
Polyclonal Antibody to Pyrin (human) (AL196)
SYNONYMS
anti-MEFV (human) PAb (AL196)
anti-Mediterranean Fever Protein (AL196)
anti-Marenostrin (human) PAb (AL196)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Inflammasome
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-946-C100
100 µg
340.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml
PURITY DETAIL:
Epitope-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 2-29 (A
2
KTPSDHLLSTLEELVPYDFEKFKFKLQ
29
) of human pyrin (MEFV; mediterranean fever protein).
SPECIFICITY:
Recognizes human pyrin.
APPLICATION:
Immunoprecipitation
Western Blot (1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
The SPRY domain of Pyrin, mutated in familial Mediterranean fever patients, interacts with inflammasome components and inhibits proIL-1beta processing:
S. Papin, et al.; Cell Death Differ.
14
, 1457 (2007)
Abstract
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-949
Revised 29-Oct-07
Polyclonal Antibody to Pannexin-1 (human) (AT124)
SYNONYMS
anti-Pannexin-1 (human) PAb (AT124)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Inflammasome
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-949-C100
100 µg
350.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml
PURITY DETAIL:
Epitope-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to 15 aa near the C-terminus of human pannexin-1.
SPECIFICITY:
Recognizes human pannexin-1.
APPLICATION:
Western Blot (1:1’000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
Pannexin-1 belongs to a new family of membrane proteins with homology to invertebrate gap junction proteins innexins. Pannexin-1 has recently been involved in the NALP (inflammasome)-mediated caspase-1 activation and IL-1β secretion.
MANUFACTURER
Manufactured b
