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Kinases in T Cell Signal Transduction / Related Products
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ALX-385-021 Revised 23-Oct-07
4'-Amino-6-hydroxyflavone
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SYNONYMS Aminogenistein
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Flavones
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ALX-385-021-M001   1 mg 140.00 USD Add To Cart
Product Specification
FORMULA: C15H11NO3
MW: 253.3
PURITY: ≥95%
APPEARANCE: Yellow powder.
SOLUBILITY: Soluble in DMSO or methanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
Inhibitor of the p56lck protein-tyrosine kinase.
Product Specific Literature References
Synthesis and protein-tyrosine kinase inhibitory activities of flavonoid analogues: M. Cushman, et al.; J. Med. Chem. 34, 798 (1991) Abstract
Expression of p56lck in B-cell neoplasias: A. Von Knethen, et al.; Leuk. Lymphoma 26, 551 (1997) Abstract
Further Categories Containing This Product:
Tyrosine Kinase Inhibitors • Src Family Kinases / Related Products • Kinases in T Cell Signal Transduction / Related Products
 
 
ALX-270-181 Revised 28-Jul-08
Damnacanthal
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SYNONYMS 3-Hydroxy-1-methoxyanthraquinone-2-aldehyde
PRODUCT LINE Immunology
PRODUCT CATEGORY Kinases in T Cell Signal Transduction / Related Products
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ALX-270-181-M001   1 mg 165.00 USD Add To Cart
Product Specification
FORMULA: C16H10O5
MW: 282.3
CAS NUMBER: 477-84-9
PURITY: ≥95% (HPLC)
APPEARANCE: Yellow to orange solid.
SOLUBILITY: 10mg/ml soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
Potent and selective inhibitor of p56lck tyrosine kinase activity. Inhibition of p56lck by damnacanthal appears to be reversible, not time-dependent and not significantly affected by the presence of sulfhydryl reagents.
Product Specific Literature References
S. Nonomura; J. Pharm. Soc. Jpn. 75, 219 (1955)
N.R. Ayyangar, et al.; Tetrahedron 6, 331 (1959)
Effects of three compounds extracted from Morinda lucida on Plasmodium falciparum: K.Koumaglo, et al.; Planta Medica 58, 533 (1992) Abstract
Induction of normal phenotypes in ras-transformed cells by damnacanthal from Morinda citrifolia: T. Hiramatsu, et al.; Cancer Lett. 73, 161 (1993) Abstract
Damnacanthal is a highly potent, selective inhibitor of p56lck tyrosine kinase activity: C.R. Faltynek, et al.; Biochemistry 34, 12404 (1995) Abstract
Further Categories Containing This Product:
Src Family Kinases / Related Products • Tyrosine Kinase Inhibitors
 
 
ALX-350-057 Revised 20-Feb-08
Emodin
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SYNONYMS 6-Methyl-1,3,8-trihydroxyanthraquinone
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Protein Kinase Inhibitors
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ALX-350-057-M025   25 mg 45.00 USD Add To Cart
Product Specification
FORMULA: C15H10O5
MW: 270.2
CAS NUMBER: 518-82-1
MERCK INDEX: 14: 3561
RTECS: CB7920600
SOURCE/HOST: Isolated from Frangula bark.
PURITY: ≥96% (HPLC)
APPEARANCE: Orange to red solid.
SOLUBILITY: Soluble in DMSO, 100% ethanol or 1N dilute aqueous ammonia; insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HAZARD: IRRITANT.

Product Description
Inhibitor of p56lck tyrosine kinase. Has mutagenic and genotoxic effects, mainly in bacterial systems. Anticancer agent. Exhibits anti-proliferative effects in various cancer cell lines by efficient induction of apoptosis. Has inhibitory effects on angiogenic and metastasis regulatory processes. Anti-inflammatory compound. Suppresses NF-κB activation. 
Product Specific Literature References
Emodin, a protein tyrosine kinase inhibitor from Polygonum cuspidatum: H. Jayasuriya, et al.; J. Nat. Prod. 55, 696 (1992) Abstract
Selective inhibition of the growth of ras-transformed human bronchial epithelial cells by emodin, a protein-tyrosine kinase inhibitor: T.C.K. Chan, et al.; BBRC 193, 1152 (1993) Abstract
Molecular mechanism of emodin action: transition from laxative ingredient to an antitumor agent: G. Srinivas, et al.; Med. Res. Rev. 27, 591 (2007), (Review) Abstract
Further Categories Containing This Product:
Tyrosine Kinase Inhibitors • Other Toxins • Src Family Kinases / Related Products • Kinases in T Cell Signal Transduction / Related Products • Natural Products - Anti-inflammatory Agents • Natural Products - NF-kB Pathway Inhibitors • Natural Products - Antitumor Reagents • Natural Products - Apoptosis Inducers & Inhibitors • NF-kB Pathway Inhibitors
 
 
ALX-804-479 Revised 10-Jun-08
Monoclonal Antibody to Lck (human) (LCK-01)
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PRODUCT LINE Immunology
PRODUCT CATEGORY Kinases in T Cell Signal Transduction / Related Products
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ALX-804-479-C100   100 µg 240.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: LCK-01
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE)
FORMULATION: Liquid. Affinity purified antibody in PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 22-36 of human Lck.
SPECIFICITY: Recognizes human Lck. Does not cross-react with mouse Lck.
APPLICATION: Flow Cytometry
Immunocytochemistry
Immunoprecipitation
Western Blot
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells: M. Cebecauer, et al.; BBRC 243, 706 (1998) Abstract
A potential role for protein tyrosine kinase p56(lck) in rheumatoid arthritis synovial fluid T lymphocyte hyporesponsiveness: P. Romagnoli, et al.; Int. Immunol. 13, 305 (2001) Abstract; Full Text
Further Categories Containing This Product:
Autoimmunity & Self Tolerance Other Products • Src Family Kinases / Related Products • Monoclonal Antibodies
 
 
ALX-804-565 Revised 10-Jun-08
Monoclonal Antibody to Lyn (LYN-01)
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PRODUCT LINE Immunology
PRODUCT CATEGORY Kinases in T Cell Signal Transduction / Related Products
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ALX-804-565-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: LYN-01
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml.
PURITY: >95% (SDS-PAGE).
FORMULATION: Liquid. Protein A affinity purified antibody in PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Recombinant human Lyn (aa 8-238).
SPECIFICITY: Recognizes human, mouse and rat Lyn.
APPLICATION: Immunocytochemistry
Immunoprecipitation
Western Blot
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Specific Literature References
Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains: L. Draberova, et al.; Immunology 87, 141 (1996) Abstract
Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors: M. Amoui, et al.; Eur. J. Immunol. 27, 321 (1997) Abstract
Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation: M. Kovarova, et al.; Mol. Cell. Biol. 21, 8318 (2001) Abstract
Further Categories Containing This Product:
Src Family Kinases / Related Products • Monoclonal Antibodies
 
 
ALX-804-480 Revised 09-May-07
Monoclonal Antibody to Syk (SYK-01)
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PRODUCT LINE Immunology
PRODUCT CATEGORY Kinases in T Cell Signal Transduction / Related Products
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ALX-804-480-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: SYK-01
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Recombinant fragment (aa 5-360) of human Syk.
SPECIFICITY: Recognizes human, mouse and rat Syk.
APPLICATION: Immunocytochemistry
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors: M. Amoui, et al.; Eur. J. Immunol. 27, 321 (1997) Abstract
Protein tyrosine kinase Syk is involved in Thy-1 signaling in rat basophilic leukemia cells: P. Tolar, et al.; Eur. J. Immunol. 27, 3389 (1997) Abstract
Requirement for a negative charge at threonine 60 of the FcRgamma for complete activation of Syk: P.G. Swann, et al.; J. Biol. Chem. 274, 23068 (1999) Abstract; Full Text
The Src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH(2)-terminal kinase 1: R. Arudchandran, et al.; J. Exp. Med. 191, 47 (2000) Abstract; Full Text
Vav1 regulates phospholipase cgamma activation and calcium responses in mast cells: T.S. Manetz, et al.; Mol. Cell. Biol. 21, 3763 (2001) Abstract; Full Text
Structure-function analysis of Lyn kinase association with lipid rafts and initiation of early signaling events after Fcepsilon receptor I aggregation: M. Kovarova, et al.; Mol. Cell. Biol. 21, 8318 (2001) Abstract; Full Text
A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fc epsilon receptor: I. Halova, et al.; Int. Immunol. 14, 213 (2002) Abstract
Fyn kinase initiates complementary signals required for IgE-dependent mast cell degranulation: V. Parravicini, et al.; Nat. Immunol. 3, 741 (2002) Abstract
Further Categories Containing This Product:
Innate Immunity Other Products • Monoclonal Antibodies
 
 
ALX-805-716 Revised 03-Nov-06
Monoclonal Antibody to ZAP-70 (human) (ZAP-03)
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PRODUCT LINE Immunology
PRODUCT CATEGORY Kinases in T Cell Signal Transduction / Related Products
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ALX-805-716-C100   100 µg 225.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: ZAP-03
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Recombinant C-terminal part (160 aa) of human ZAP-70.
SPECIFICITY: Recognizes human ZAP-70. Detects a band of ~70kDa by Western blot.
APPLICATION: Flow Cytometry
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Autoimmunity & Self Tolerance Other Products • Monoclonal Antibodies
 
 
ALX-270-202 Revised 07-Oct-08
Piceatannol
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SYNONYMS 3,4,3',5'-Tetrahydroxy-trans-stilbene
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Protein Kinase Inhibitors
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ALX-270-202-M001   1 mg 22.00 USD Add To Cart
ALX-270-202-M005   5 mg 62.00 USD Add To Cart
ALX-270-202-M010   10 mg 105.00 USD Add To Cart
ALX-270-202-M050   50 mg 375.00 USD Add To Cart
Product Specification
FORMULA: C14H12O4
MW: 244.3
CAS NUMBER: 10083-24-6
SOURCE/HOST: Synthetic.
PURITY: ≥98%
APPEARANCE: Off-white to light pink crystalline solid.
SOLUBILITY: Soluble in DMSO, dimethyl formamide, 100% ethanol, methanol, acetone or ethyl acetate; slightly soluble in water (1mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Unstable in sodium hydroxide.
HANDLING: Protect from light and oxygen.

Product Description
Originally isolated from Euphorbia lagascae. Selective protein tyrosine kinase Syk inhibitor. Activator of human deacetylase SIRT1.
Product Specific Literature References
Synthesis and evaluation of stilbene and dihydrostilbene derivatives as potential anticancer agents that inhibit tubulin polymerization: M. Cushman, et al.; J. Med. Chem. 34, 2579 (1991) Abstract
Inhibition of mast cell Fc epsilon R1-mediated signaling and effector function by the Syk-selective inhibitor, piceatannol: J.M. Oliver, et al.; J. Biol. Chem. 269, 29697 (1994) Abstract; Full Text
The tyrosine phosphorylation of a p72syk-like protein in activated murine resident peritoneal macrophages: K.I. Abu-Lawi & B.M. Sultzer; Cell Mol. Biol. Res. 41, 49 (1995) Abstract
Calcium signaling in endothelial cells involves activation of tyrosine kinases and leads to activation of mitogen-activated protein kinases: I. Fleming, et al.; Circul. Res. 76, 522 (1995) Abstract
Coronary arteriolar flow-induced vasodilation signals through tyrosine kinase: J.M. Muller, et al.; Am. J. Physiol. 270, H1878 (1996) Abstract
Syk, activated by cross-linking the B-cell antigen receptor, localizes to the cytosol where it interacts with and phosphorylates alpha-tubulin on tyrosine: J.D. Peters, et al.; J. Biol. Chem. 271, 4755 (1996) Abstract; Full Text
Src-regulated extracellular signal-related kinase and Syk-regulated c-Jun N-terminal kinase pathways act in conjunction to induce IL-1 synthesis in response to microtubule disruption in HL60 cells: B. Cambien, et al.; J. Immunol. 163, 5079 (1999) Abstract; Full Text
FcepsilonRI-mediated antigen endocytosis turns interferon-gamma-treated mouse mast cells from inefficient into potent antigen-presenting cells: C .Tkaczyk, et al.; Immunology 97, 333 (1999) Abstract
Piceatannol, a stilbene phytochemical, inhibits mitochondrial F0F1-ATPase activity by targeting the F1 complex: J. Zheng & V.D. Ramirez; BBRC 261, 499 (1999) Abstract
Microarray analysis of piceatannol-induced changes in gene expression in human gastric cancer cells : D. Jeoung, et al.; Biotechnology Lett. 24, 463 (2002) Abstract; Full Text
Piceatannol, a Syk-selective tyrosine kinase inhibitor, attenuated antigen challenge of guinea pig airways in vitro: C.J. Seow, et al.; Eur. J. Pharmacol. 443, 189 (2002) Abstract
Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan: K.T. Howitz, et al.; Nature 425, 191 (2003)
Further Categories Containing This Product:
Kinases in T Cell Signal Transduction / Related Products • B Cell Receptor [BCR] & Co-Receptors / Related Products • Tyrosine Kinase Inhibitors • Sirtuins / Related Products • Stilbenoids
 
 
ALX-210-827 Revised 18-Oct-07
Polyclonal Antibody to Lck (human)
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PRODUCT LINE Immunology
PRODUCT CATEGORY Kinases in T Cell Signal Transduction / Related Products
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ALX-210-827-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Contains 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 38-57 (R38NGSEVRDPLVTYEGSNPPA57) of human Lck.
SPECIFICITY: Recognizes human Lck.
APPLICATION: Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
The structure of the mu/pseudo light chain complex on human pre-B cells is consistent with a function in signal transduction: G.S. Brouns, et al.; Eur. J Immunol. 23, 1088 (1993) Abstract
Further Categories Containing This Product:
Autoimmunity & Self Tolerance Other Products • Src Family Kinases / Related Products • Polyclonal Antibodies
 
 
ALX-210-572 Revised 03-Nov-06
Polyclonal Antibody to ZAP-70 (human)
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PRODUCT LINE Immunology
PRODUCT CATEGORY Kinases in T Cell Signal Transduction / Related Products
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ALX-210-572-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Recombinant fusion protein corresponding to C-terminal part of human ZAP-70.
SPECIFICITY: Recognizes C-terminus of human ZAP-70.
APPLICATION: Western Blot (1:1’000; use standard reducing SDS-sample buffer)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Autoimmunity & Self Tolerance Other Products • Polyclonal Antibodies
 
 
ALX-270-177 Revised 17-Apr-07
PP1
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SYNONYMS 4-Amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo[3,4-d]-pyrimidine
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Tyrosine Kinase Inhibitors
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ALX-270-177-M001   1 mg 70.00 USD Add To Cart
ALX-270-177-M005   5 mg 280.00 USD Add To Cart
Product Specification
FORMULA: C16H19N5
MW: 281.4
CAS NUMBER: 172889-26-8
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, slightly soluble in 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Potent inhibitor of Src-family tyrosine kinases. Inhibits p56lck and p59fynT (IC50=5nM and 6nM respectively). Displays >8'000-fold selectivity over ZAP-70 and Jak2. Also moderately inhibits p38, CSK, PDGF receptors, RET-derived oncoproteins, c-Kit and Bcr-Abl.
Product Specific Literature References
Discovery of a novel, potent, and Src family-selective tyrosine kinase inhibitor. Study of Lck- and FynT-dependent T cell activation: J.H. Hanke, et al.; J. Biol. Chem. 271, 695 (1996) Abstract; Full Text
Signal characteristics of G protein-transactivated EGF receptor: H. Daub, et al.; EMBO J. 16, 7032 (1997) Abstract; Full Text
Src family-selective tyrosine kinase inhibitor, PP1, inhibits both Fc epsilonRI- and Thy-1-mediated activation of rat basophilic leukemia cells: M. Amoui, et al.; Eur. J. Immunol. 27, 1881 (1997) Abstract
The urokinase-type plasminogen activator receptor mediates tyrosine phosphorylation of focal adhesion proteins and activation of mitogen-activated protein kinase in cultured endothelial cells: H. Tang, et al.; J. Biol. Chem. 273, 18268 (1998) Abstract; Full Text
Tyrosine kinases of the Src family participate in signaling to MAP kinase from both Gq and Gi-coupled receptors: T. Igishi and J.S. Gutkind; BBRC 244, 5 (1998) Abstract
Morphological differentiation of oligodendrocytes requires activation of Fyn tyrosine kinase: D.J. Osterhout, et al.; J. Cell Biol. 145, 1209 (1999) Abstract; Full Text
Structural basis for selective inhibition of Src family kinases by PP1: Y. Liu, et al.; Chem. Biol. 6, 671 (1999) Abstract
Reactive oxygen species activate p90 ribosomal S6 kinase via Fyn and Ras: J. Abe, et al.; J. Biol. Chem. 275, 1739 (2000) Abstract; Full Text
Cytokine-driven proliferation and differentiation of human naive, central memory, and effector memory CD4(+) T cells: J. Geginat, et al.; J. Exp. Med. 194, 1711 (2001) Abstract; Full Text
The kinase inhibitor PP1 blocks tumorigenesis induced by RET oncogenes: F. Carlomagno, et al.; Cancer Res. 62, 1077 (2002) Abstract; Full Text
The specificities of protein kinase inhibitors: an update: J. Bain, et al.; Biochem. J. 371, 199 (2003) Abstract; Full Text
The Src-selective kinase inhibitor PP1 also inhibits Kit and Bcr-Abl tyrosine kinases: L. Tatton, et al.; J. Biol. Chem. 278, 4847 (2003) Abstract; Full Text
Further Categories Containing This Product:
Src Family Kinases / Related Products • Kinases in T Cell Signal Transduction / Related Products
 
 
ALX-270-233 Revised 10-Jan-07
PP2